RLG00000019349

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001441
Physical Location & Seq
Reverse (-)
50635909 .. 50640426
4518 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019349

Sequence Viewer

Length: 1311 bp
ATGATGGCTCTCCGACTGAGAACCAACCACCGAACCCTCACAAACCCATCTCTCATTCACCTCTTCTCGACGTCCTCCTCAGACCCATCCGACTCAGCCGACCCAACTGAACCCAACCCCCATTCCGACTCCTCCTCTCTCTCCAGTTACTTCAGCGATGTCAAAGCCAGCCTCAGGCAACAGTCACAGTCACAGCAACAACACAGCAGACCCACTTCATACTCACAGCAACAACACTTCAGACCCACTTCTCAGAACAACAACCCTTCTCTCTCCCGGCCTTCCAAGGCTGCCTCCTTGGAGGAAATCCGCAAAAACCTTGCGGAGTTTCGGCTTCGATCCATTGGGCCCAACCCCACTGAATCAAACTCTGCACCTCCACCTCCACCTCCGAACCCCATTTCGTTTGAGAAGCTCTATAACAAAGGAGGCGTGAGCAGCCGCAAGATGGAGTTTCAGACGGTTCGGGAGAGTCTGAAGCTCATAAGGCCCAATGCGAATGAGCAGAATGAGAGGAAGGGTGGGGGATTGTCGTCGTTGAGGTTCAAAAGTTTGGATATGAATCCGAGCCAAGATGTGGGTGTTAGGAAGGGACCACTTACAAGTTTGTTTGATAAGCAGAAGGAGAGAAGTGGAGAGAAGAGCTCGGATGCATTGAAGACTGAGTTTGTGAAGATTTATAGCTATAAAGAGTTGGGGGAGAAGCTGAGGAAGTTGAGGCCCGAGGAACGGAAGGAAGGGGGCGGGTTTACATTGGGGGAGATGAGTGAGAGGTTGGTCAAGTTAAGGAAGATGGAAGAGAATGAGTCCGAGTCTCGGGTTGGGGGATTGAATAATGACGACTTGAAGCAGAGCTTGTTGAAGATATCCAGAGAGGAGAAGCATAGGAAAACTCCAATGCAGAGGCTTCATATGATAGATATAGGTCAAACTCCTGATTATATGATGAAACCCCCAAAGGAAGGCTTGGTTGAGAAGTATTTCCATCCAGATAACATGTCTTCTGCAGAAAAGCTGAAAATTAAGCTTGGCAAAGTTAGAGATGAATTTAAAATGTCAGAGTCAGATTGTGGTTCTGCACGAGTTCAAGTGGCACAACTCACAACTAAGATCAAGCATCTATCTTCAGTTCTACACAAAAAGGATAAGCATTCACTTAAGGGTCTTCAAGCAATGGTGCAGAGAAGGAAGAAGCTATTCAAGTATCTCCGAAGAACTGACTGGGACTCTTACTGCCTTGCTCTCTCCAAACTTGGTCTCCGGGACACGCCAGATGCCAAACAAGAGTACAAGAAACATTACAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

437

Amino Acids

49.76

Weight (kDa)

9.85

Isoelectric Point (pI)

55.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S15 PF00312 346 - 421 2.1e-22 Ribosomal protein S15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 74
AccB7I CCANNNNNTGG 1 cut(s) 577
AciI CCGC 4 cut(s) 310, 323, 442, 744
AclWI GGATC 1 cut(s) 333
AcsI RAATTY 1 cut(s) 1046
AcuI CTGAAG 4 cut(s) 136, 223, 497, 1110
AcyI GRCGYC 1 cut(s) 71
AfaI GTAC 1 cut(s) 1289
AfiI CCNNNNNNNGG 6 cut(s) 174, 448, 577, 729, 816, 962
AflII CTTAAG 1 cut(s) 1157
AflIII ACRYGT 1 cut(s) 996
AgsI TTSAA 8 cut(s) 547, 658, 832, 847, 862, 1088, 1169, 1201
AluBI AGCT 9 cut(s) 415, 481, 645, 684, 706, 855, 1015, 1027, 1195
AluI AGCT 9 cut(s) 415, 481, 645, 684, 706, 855, 1015, 1027, 1195
Alw21I GWGCWC 1 cut(s) 647
Alw26I GTCTC 2 cut(s) 819, 1262
AlwI GGATC 1 cut(s) 333
Ama87I CYCGRG 2 cut(s) 722, 816
AoxI GGCC 4 cut(s) 278, 347, 488, 719
ApaI GGGCCC 1 cut(s) 351
ApeKI GCWGC 2 cut(s) 290, 438
ApoI RAATTY 1 cut(s) 1046
Asp700I GAANNNNTTC 2 cut(s) 980, 1196
AspS9I GGNCC 5 cut(s) 347, 348, 489, 593, 720
AsuC2I CCSGG 2 cut(s) 277, 1262
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 2 cut(s) 722, 816
AvaII GGWCC 1 cut(s) 593
AxyI CCTNAGG 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 351
BanII GRGCYC 2 cut(s) 351, 647
BauI CACGAG 1 cut(s) 1080
BbsI GAAGAC 3 cut(s) 665, 993, 1157
Bbv12I GWGCWC 1 cut(s) 647
BbvCI CCTCAGC 1 cut(s) 707
BbvI GCAGC 2 cut(s) 277, 450
BccI CCATC 5 cut(s) 55, 94, 442, 787, 993
BcnI CCSGG 2 cut(s) 277, 1262
BcoDI GTCTC 2 cut(s) 819, 1262
BfmI CTRYAG 1 cut(s) 1005
BfrI CTTAAG 1 cut(s) 1157
BisI GCNGC 3 cut(s) 291, 439, 442
BlsI GCNGC 3 cut(s) 292, 440, 443
Bme1390I CCNGG 2 cut(s) 277, 1262
Bme18I GGWCC 1 cut(s) 593
BmeT110I CYCGRG 2 cut(s) 722, 816
BmgT120I GGNCC 5 cut(s) 347, 348, 489, 593, 720
BmiI GGNNCC 2 cut(s) 349, 594
BmrFI CCNGG 2 cut(s) 277, 1262
BmrI ACTGGG 1 cut(s) 1231
BmsI GCATC 3 cut(s) 640, 1126, 1264
BmuI ACTGGG 1 cut(s) 1231
BpiI GAAGAC 3 cut(s) 665, 993, 1157
BplI GAGNNNNNCTC 2 cut(s) 629, 661
BpmI CTGGAG 1 cut(s) 127
Bpu10I CCTNAGC 1 cut(s) 707
BpuMI CCSGG 2 cut(s) 277, 1262
BsaBI GATNNNNATC 1 cut(s) 561
BsaHI GRCGYC 1 cut(s) 71
BsaI GGTCTC 1 cut(s) 1262
BsaJI CCNNGG 3 cut(s) 285, 297, 723
BsaXI ACNNNNNCTCC 2 cut(s) 1242, 1272
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 448, 577, 729, 816, 962
Bse1I ACTGG 2 cut(s) 144, 1226
Bse21I CCTNAGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 1179
Bse8I GATNNNNATC 1 cut(s) 561
BseDI CCNNGG 3 cut(s) 285, 297, 723
BseGI GGATG 3 cut(s) 86, 655, 985
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 6 cut(s) 174, 448, 577, 729, 816, 962
BseMI GCAATG 1 cut(s) 1179
BseMII CTCAG 7 cut(s) 8, 93, 108, 187, 266, 654, 698
BseNI ACTGG 2 cut(s) 144, 1226
BseRI GAGGAG 4 cut(s) 67, 121, 124, 890
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 2 cut(s) 277, 450
BsgI GTGCAG 3 cut(s) 357, 1062, 1199
BshFI GGCC 4 cut(s) 280, 349, 490, 721
BsiHKAI GWGCWC 1 cut(s) 647
BsiHKCI CYCGRG 2 cut(s) 722, 816
BsiSI CCGG 2 cut(s) 277, 1261
BslFI GGGAC 3 cut(s) 606, 1238, 1277
BslI CCNNNNNNNGG 6 cut(s) 174, 448, 577, 729, 816, 962
BsmAI GTCTC 2 cut(s) 819, 1262
BsmFI GGGAC 3 cut(s) 606, 1238, 1277
BsmI GAATGC 1 cut(s) 1150
BsnI GGCC 4 cut(s) 280, 349, 490, 721
Bso31I GGTCTC 1 cut(s) 1262
BsoBI CYCGRG 2 cut(s) 722, 816
Bsp120I GGGCCC 1 cut(s) 347
Bsp1286I GDGCHC 2 cut(s) 351, 647
Bsp143I GATC 2 cut(s) 338, 1110
BspACI CCGC 4 cut(s) 310, 323, 442, 744
BspANI GGCC 4 cut(s) 280, 349, 490, 721
BspCNI CTCAG 7 cut(s) 9, 92, 107, 186, 265, 655, 699
BspLI GGNNCC 2 cut(s) 349, 594
BspMAI CTGCAG 1 cut(s) 1009
BspPI GGATC 1 cut(s) 333
BspQI GCTCTTC 1 cut(s) 635
BspTI CTTAAG 1 cut(s) 1157
BspTNI GGTCTC 1 cut(s) 1262
BsrDI GCAATG 1 cut(s) 1179
BsrI ACTGG 2 cut(s) 144, 1226
BssECI CCNNGG 3 cut(s) 285, 297, 723
BssMI GATC 2 cut(s) 338, 1110
BssNI GRCGYC 1 cut(s) 71
BssSI CACGAG 1 cut(s) 1080
BssT1I CCWWGG 2 cut(s) 285, 297
Bst2BI CACGAG 1 cut(s) 1080
Bst4CI ACNGT 3 cut(s) 183, 189, 463
Bst6I CTCTTC 3 cut(s) 68, 635, 792
BstACI GRCGYC 1 cut(s) 71
BstAFI CTTAAG 1 cut(s) 1157
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 8 cut(s) 17, 79, 94, 173, 252, 663, 707, 1107
BstF5I GGATG 3 cut(s) 86, 655, 985
BstKTI GATC 2 cut(s) 341, 1113
BstMAI GTCTC 2 cut(s) 819, 1262
BstMBI GATC 2 cut(s) 338, 1110
BstMWI GCNNNNNNNGC 2 cut(s) 438, 487
BstNSI RCATGY 1 cut(s) 1000
BstSCI CCNGG 2 cut(s) 275, 1260
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 277, 450
BstV2I GAAGAC 3 cut(s) 665, 993, 1157
Bsu36I CCTNAGG 1 cut(s) 173
BsuRI GGCC 4 cut(s) 280, 349, 490, 721
BtgZI GCGATG 1 cut(s) 171
BtsCI GGATG 3 cut(s) 86, 655, 985
BtsIMutI CAGTG 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 5 cut(s) 347, 348, 489, 593, 720
Csp6I GTAC 1 cut(s) 1288
CviAII CATG 1 cut(s) 997
CviQI GTAC 1 cut(s) 1288
DdeI CTNAG 8 cut(s) 17, 79, 94, 173, 252, 663, 707, 1107
DpnI GATC 2 cut(s) 340, 1112
DpnII GATC 2 cut(s) 338, 1110
DraI TTTAAA 1 cut(s) 1051
Eam1104I CTCTTC 3 cut(s) 68, 635, 792
EarI CTCTTC 3 cut(s) 68, 635, 792
Ecl136II GAGCTC 1 cut(s) 645
Eco130I CCWWGG 2 cut(s) 285, 297
Eco24I GRGCYC 2 cut(s) 351, 647
Eco31I GGTCTC 1 cut(s) 1262
Eco32I GATATC 1 cut(s) 867
Eco47I GGWCC 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 645
Eco57I CTGAAG 4 cut(s) 136, 223, 497, 1110
Eco81I CCTNAGG 1 cut(s) 173
Eco88I CYCGRG 2 cut(s) 722, 816
EcoICRI GAGCTC 1 cut(s) 645
EcoRV GATATC 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 285, 297
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 2 cut(s) 351, 647
ErhI CCWWGG 2 cut(s) 285, 297
FaeI CATG 1 cut(s) 1000
FalI AAGNNNNNCTT 4 cut(s) 839, 871, 950, 982
FaqI GGGAC 3 cut(s) 606, 1238, 1277
FatI CATG 1 cut(s) 996
FauI CCCGC 1 cut(s) 737
FauNDI CATATG 1 cut(s) 912
Fnu4HI GCNGC 3 cut(s) 291, 439, 442
FokI GGATG 3 cut(s) 73, 662, 972
FriOI GRGCYC 2 cut(s) 351, 647
Fsp4HI GCNGC 3 cut(s) 291, 439, 442
GluI GCNGC 3 cut(s) 291, 439, 442
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 4 cut(s) 280, 349, 490, 721
HapII CCGG 2 cut(s) 277, 1261
Hin1I GRCGYC 1 cut(s) 71
Hin1II CATG 1 cut(s) 1000
HindIII AAGCTT 1 cut(s) 1025
HinfI GANTC 9 cut(s) 92, 128, 362, 472, 562, 806, 812, 1061, 1226
HpaII CCGG 2 cut(s) 277, 1261
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 750
Hpy188III TCNNGA 5 cut(s) 67, 467, 870, 935, 989
Hpy8I GTNNAC 1 cut(s) 750
Hpy99I CGWCG 2 cut(s) 73, 538
HpyAV CCTTC 9 cut(s) 276, 291, 511, 583, 616, 727, 731, 956, 1179
HpyCH4III ACNGT 3 cut(s) 183, 189, 463
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 6 cut(s) 374, 653, 901, 1007, 1079, 1180
HpyF10VI GCNNNNNNNGC 2 cut(s) 438, 487
HpyF3I CTNAG 8 cut(s) 17, 79, 94, 173, 252, 663, 707, 1107
HpySE526I ACGT 1 cut(s) 71
Hsp92I GRCGYC 1 cut(s) 71
Hsp92II CATG 1 cut(s) 1000
Kzo9I GATC 2 cut(s) 338, 1110
LguI GCTCTTC 1 cut(s) 635
Lsp1109I GCAGC 2 cut(s) 277, 450
LweI GCATC 3 cut(s) 640, 1126, 1264
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 3 cut(s) 146, 183, 189
MalI GATC 2 cut(s) 340, 1112
MboI GATC 2 cut(s) 338, 1110
MhlI GDGCHC 2 cut(s) 351, 647
MluCI AATT 3 cut(s) 1020, 1046, 1306
MlyI GAGTC 7 cut(s) 86, 122, 481, 815, 821, 1070, 1220
MmeI TCCRAC 3 cut(s) 37, 114, 150
Mph1103I ATGCAT 1 cut(s) 655
MroXI GAANNNNTTC 2 cut(s) 980, 1196
MseI TTAA 4 cut(s) 785, 1023, 1050, 1158
MspCI CTTAAG 1 cut(s) 1157
MspI CCGG 2 cut(s) 277, 1261
MspR9I CCNGG 2 cut(s) 277, 1262
Mva1269I GAATGC 1 cut(s) 1150
MwoI GCNNNNNNNGC 2 cut(s) 438, 487
NciI CCSGG 2 cut(s) 277, 1262
NdeI CATATG 1 cut(s) 912
NdeII GATC 2 cut(s) 338, 1110
NlaIII CATG 1 cut(s) 1000
NlaIV GGNNCC 2 cut(s) 349, 594
NmuCI GTSAC 2 cut(s) 183, 189
NsiI ATGCAT 1 cut(s) 655
NspI RCATGY 1 cut(s) 1000
PciI ACATGT 1 cut(s) 996
PciSI GCTCTTC 1 cut(s) 635
PctI GAATGC 1 cut(s) 1150
PdmI GAANNNNTTC 2 cut(s) 980, 1196
PfeI GAWTC 2 cut(s) 362, 562
PflMI CCANNNNNTGG 1 cut(s) 577
PfoI TCCNGGA 1 cut(s) 1260
PkrI GCNGC 3 cut(s) 292, 440, 443
PleI GAGTC 7 cut(s) 86, 122, 480, 814, 820, 1069, 1220
PpsI GAGTC 7 cut(s) 86, 122, 480, 814, 820, 1069, 1220
PscI ACATGT 1 cut(s) 996
Psp124BI GAGCTC 1 cut(s) 647
PspN4I GGNNCC 2 cut(s) 349, 594
PspOMI GGGCCC 1 cut(s) 347
PspPI GGNCC 5 cut(s) 347, 348, 489, 593, 720
PstI CTGCAG 1 cut(s) 1009
RsaI GTAC 1 cut(s) 1289
RsaNI GTAC 1 cut(s) 1288
SacI GAGCTC 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 635
SaqAI TTAA 4 cut(s) 785, 1023, 1050, 1158
SatI GCNGC 3 cut(s) 291, 439, 442
Sau3AI GATC 2 cut(s) 338, 1110
Sau96I GGNCC 5 cut(s) 347, 348, 489, 593, 720
SchI GAGTC 7 cut(s) 86, 122, 481, 815, 821, 1070, 1220
ScrFI CCNGG 2 cut(s) 277, 1262
SduI GDGCHC 2 cut(s) 351, 647
SfaNI GCATC 3 cut(s) 640, 1126, 1264
SfcI CTRYAG 1 cut(s) 1005
SinI GGWCC 1 cut(s) 593
SmlI CTYRAG 1 cut(s) 1157
SmoI CTYRAG 1 cut(s) 1157
Sse9I AATT 3 cut(s) 1020, 1046, 1306
SsiI CCGC 4 cut(s) 310, 323, 442, 744
SstI GAGCTC 1 cut(s) 647
StyD4I CCNGG 2 cut(s) 275, 1260
StyI CCWWGG 2 cut(s) 285, 297
TaaI ACNGT 3 cut(s) 183, 189, 463
TaiI ACGT 1 cut(s) 74
TaqI TCGA 2 cut(s) 68, 337
TasI AATT 3 cut(s) 1020, 1046, 1306
TatI WGTACW 1 cut(s) 1287
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 2 cut(s) 362, 562
Tru1I TTAA 4 cut(s) 785, 1023, 1050, 1158
Tru9I TTAA 4 cut(s) 785, 1023, 1050, 1158
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 2 cut(s) 183, 189
TseI GCWGC 2 cut(s) 290, 438
Tsp45I GTSAC 2 cut(s) 183, 189
TspDTI ATGAA 5 cut(s) 207, 575, 899, 962, 1059
TspGWI ACGGA 1 cut(s) 745
TspRI CASTG 1 cut(s) 364
Van91I CCANNNNNTGG 1 cut(s) 577
Vha464I CTTAAG 1 cut(s) 1157
VpaK11BI GGWCC 1 cut(s) 593
XapI RAATTY 1 cut(s) 1046
XceI RCATGY 1 cut(s) 1000
XmnI GAANNNNTTC 2 cut(s) 980, 1196
ZraI GACGTC 1 cut(s) 72
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.