Rmu_sc0003573.1_g000004

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003573.1
OG0001441
Physical Location & Seq
Reverse (-)
14372 .. 20446
6075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003573.1_g000004.1.cds

Sequence Viewer

Length: 1248 bp
atgatggctctccgactgagaaccaaccagcaccgaaccctcacaaacccatcatctctcatccacctcttctccacttcctcctcagacccagccgactcaacatacccaactgaacccaatccccagtccgactcttcctctctctccaactacttcagcgatgtcaaagccagcctcaggcaacagcaacaacacaggggcagacccacttctcagaacaacaacccttttctctcccgcccttccaaggccgcctccttggaggaaatcagcaacaagcttgcggagtttcggcttcgaaaccccactgcacctccacctccacctccgaaccccatttcgtttgagaagctctataacaaagacgtgagcagccgcaagatggagtttcaggcgattcgggagagtctgaagcttataaagcccaatgccgatgagcggaatgagaggaagggtgagggtttgccgttgttgaggcttcggaaaagtttggatatgaatcccagccaggatgcgggtgttagggagggagcacttccgaatttgttttataaggagaaggagagaaacggagacaagagctcggatgcattgaagaccgagtttgtgaagatttatagctataaagagttgggggagaagctgagcgagttgaggcccgaggaggggaaggagtttacattgggggagttgagtgagaggttggtcaagttgaggatgatggaagagaaagagtctgagtctcgggttgggggagtgtttgtggacgtgaggcacagcttggaggccttgaagatatcccaacaagaggagaagcataggaaaacttcgaggcagaggcttcgtatcatagatataggtcaaactcctgagtatatgatgaaacccccaaaggaaggcttggttgagaagtatttccatccagataacatgtcttctgcagaaaagctgaaaattgagcttggcaaagttagagatgaatttaaaatgtcagagtcagattgtggttctgcacgagttcaagtggcacaactcacaactaagatcaaacatctgtcttcggttttacacaaaaaggataagcattcacttaagggtcttcaagcaatggtgcagaggagaaagaagctattaaagtatcttcgaagaactgactgggactcttactgccttgttctctccaaacttggtctccgggacactccagatgtcaaacaagagtacaagaaacattacaagaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

415

Amino Acids

47.78

Weight (kDa)

9.65

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 422, 555
AccBSI CCGCTC 1 cut(s) 442
AciI CCGC 6 cut(s) 241, 255, 287, 379, 442, 518
AcsI RAATTY 2 cut(s) 544, 983
AcuI CTGAAG 2 cut(s) 142, 434
AfaI GTAC 1 cut(s) 1226
AfiI CCNNNNNNNGG 9 cut(s) 180, 250, 385, 441, 517, 668, 669, 811, 899
AflII CTTAAG 1 cut(s) 1094
AflIII ACRYGT 1 cut(s) 933
AgsI TTSAA 4 cut(s) 598, 796, 1025, 1106
AjiI CACGTC 2 cut(s) 370, 772
AjnI CCWGG 1 cut(s) 510
Alw21I GWGCWC 2 cut(s) 538, 587
Alw26I GTCTC 3 cut(s) 570, 750, 1199
Ama87I CYCGRG 2 cut(s) 662, 747
AoxI GGCC 3 cut(s) 252, 659, 789
ApeKI GCWGC 1 cut(s) 375
ApoI RAATTY 2 cut(s) 544, 983
Asp700I GAANNNNTTC 1 cut(s) 917
AspS9I GGNCC 1 cut(s) 660
AsuC2I CCSGG 1 cut(s) 1199
AsuHPI GGTGA 1 cut(s) 470
AsuII TTCGAA 2 cut(s) 301, 1147
AvaI CYCGRG 2 cut(s) 662, 747
AxyI CCTNAGG 1 cut(s) 179
BanII GRGCYC 1 cut(s) 587
BauI CACGAG 1 cut(s) 1017
BbsI GAAGAC 4 cut(s) 605, 930, 1053, 1094
Bbv12I GWGCWC 2 cut(s) 538, 587
BbvI GCAGC 1 cut(s) 387
BccI CCATC 4 cut(s) 58, 379, 718, 930
BceAI ACGGC 1 cut(s) 454
BcgI CGANNNNNNTGC 2 cut(s) 827, 861
BciT130I CCWGG 1 cut(s) 512
BcnI CCSGG 1 cut(s) 1199
BcoDI GTCTC 3 cut(s) 570, 750, 1199
BfmI CTRYAG 1 cut(s) 942
BfrI CTTAAG 1 cut(s) 1094
BisI GCNGC 3 cut(s) 255, 376, 379
BlpI GCTNAGC 1 cut(s) 647
BlsI GCNGC 3 cut(s) 256, 377, 380
Bme1390I CCNGG 2 cut(s) 512, 1199
BmeT110I CYCGRG 2 cut(s) 662, 747
BmgBI CACGTC 2 cut(s) 370, 772
BmgT120I GGNCC 1 cut(s) 660
BmrFI CCNGG 2 cut(s) 512, 1199
BmrI ACTGGG 2 cut(s) 121, 1168
BmsI GCATC 2 cut(s) 505, 580
BmuI ACTGGG 2 cut(s) 121, 1168
BpiI GAAGAC 4 cut(s) 605, 930, 1053, 1094
BpmI CTGGAG 1 cut(s) 1191
Bpu1102I GCTNAGC 1 cut(s) 647
Bpu14I TTCGAA 2 cut(s) 301, 1147
BpuMI CCSGG 1 cut(s) 1199
BsaBI GATNNNNATC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 1199
BsaJI CCNNGG 3 cut(s) 249, 261, 663
BsaXI ACNNNNNCTCC 8 cut(s) 56, 86, 301, 331, 750, 780, 1179, 1209
Bsc4I CCNNNNNNNGG 9 cut(s) 180, 250, 385, 441, 517, 668, 669, 811, 899
Bse1I ACTGG 2 cut(s) 127, 1163
Bse21I CCTNAGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 1116
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 1 cut(s) 512
BseDI CCNNGG 3 cut(s) 249, 261, 663
BseGI GGATG 5 cut(s) 60, 520, 595, 726, 922
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 9 cut(s) 180, 250, 385, 441, 517, 668, 669, 811, 899
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 7 cut(s) 8, 99, 193, 230, 638, 732, 864
BseNI ACTGG 2 cut(s) 127, 1163
BseRI GAGGAG 4 cut(s) 73, 680, 827, 1135
BseXI GCAGC 1 cut(s) 387
BseYI CCCAGC 2 cut(s) 91, 506
BsgI GTGCAG 3 cut(s) 297, 999, 1136
BshFI GGCC 3 cut(s) 254, 661, 791
BsiHKAI GWGCWC 2 cut(s) 538, 587
BsiHKCI CYCGRG 2 cut(s) 662, 747
BsiSI CCGG 1 cut(s) 1198
BslFI GGGAC 2 cut(s) 1175, 1214
BslI CCNNNNNNNGG 9 cut(s) 180, 250, 385, 441, 517, 668, 669, 811, 899
BsmAI GTCTC 3 cut(s) 570, 750, 1199
BsmFI GGGAC 2 cut(s) 1175, 1214
BsmI GAATGC 1 cut(s) 1087
BsnI GGCC 3 cut(s) 254, 661, 791
Bso31I GGTCTC 1 cut(s) 1199
BsoBI CYCGRG 2 cut(s) 662, 747
Bsp119I TTCGAA 2 cut(s) 301, 1147
Bsp1286I GDGCHC 2 cut(s) 538, 587
Bsp143I GATC 1 cut(s) 1047
Bsp1720I GCTNAGC 1 cut(s) 647
BspACI CCGC 6 cut(s) 241, 255, 287, 379, 442, 518
BspANI GGCC 3 cut(s) 254, 661, 791
BspCNI CTCAG 7 cut(s) 9, 98, 192, 229, 639, 733, 865
BspMAI CTGCAG 1 cut(s) 946
BspT104I TTCGAA 2 cut(s) 301, 1147
BspTI CTTAAG 1 cut(s) 1094
BspTNI GGTCTC 1 cut(s) 1199
BsrBI CCGCTC 1 cut(s) 442
BsrDI GCAATG 1 cut(s) 1116
BsrI ACTGG 2 cut(s) 127, 1163
BssECI CCNNGG 3 cut(s) 249, 261, 663
BssMI GATC 1 cut(s) 1047
BssSI CACGAG 1 cut(s) 1017
BssT1I CCWWGG 2 cut(s) 249, 261
Bst2BI CACGAG 1 cut(s) 1017
Bst2UI CCWGG 1 cut(s) 512
Bst6I CTCTTC 3 cut(s) 74, 142, 723
BstAFI CTTAAG 1 cut(s) 1094
BstBI TTCGAA 2 cut(s) 301, 1147
BstC8I GCNNGC 2 cut(s) 175, 285
BstDEI CTNAG 8 cut(s) 17, 85, 179, 216, 647, 741, 873, 1044
BstF5I GGATG 5 cut(s) 60, 520, 595, 726, 922
BstKTI GATC 1 cut(s) 1050
BstMAI GTCTC 3 cut(s) 570, 750, 1199
BstMBI GATC 1 cut(s) 1047
BstMWI GCNNNNNNNGC 1 cut(s) 424
BstNI CCWGG 1 cut(s) 512
BstNSI RCATGY 1 cut(s) 937
BstSCI CCNGG 2 cut(s) 510, 1197
BstSFI CTRYAG 1 cut(s) 942
BstV1I GCAGC 1 cut(s) 387
BstV2I GAAGAC 4 cut(s) 605, 930, 1053, 1094
Bsu36I CCTNAGG 1 cut(s) 179
BsuRI GGCC 3 cut(s) 254, 661, 791
BtgZI GCGATG 1 cut(s) 177
BtrI CACGTC 2 cut(s) 370, 772
BtsCI GGATG 5 cut(s) 60, 520, 595, 726, 922
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 2 cut(s) 175, 285
Cfr13I GGNCC 1 cut(s) 660
Csp6I GTAC 1 cut(s) 1225
CviAII CATG 1 cut(s) 934
CviQI GTAC 1 cut(s) 1225
DdeI CTNAG 8 cut(s) 17, 85, 179, 216, 647, 741, 873, 1044
DpnI GATC 1 cut(s) 1049
DpnII GATC 1 cut(s) 1047
DraI TTTAAA 1 cut(s) 988
Eam1104I CTCTTC 3 cut(s) 74, 142, 723
EarI CTCTTC 3 cut(s) 74, 142, 723
Ecl136II GAGCTC 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 249, 261
Eco147I AGGCCT 1 cut(s) 791
Eco24I GRGCYC 1 cut(s) 587
Eco31I GGTCTC 1 cut(s) 1199
Eco32I GATATC 1 cut(s) 801
Eco53kI GAGCTC 1 cut(s) 585
Eco57I CTGAAG 2 cut(s) 142, 434
Eco81I CCTNAGG 1 cut(s) 179
Eco88I CYCGRG 2 cut(s) 662, 747
EcoICRI GAGCTC 1 cut(s) 585
EcoRII CCWGG 1 cut(s) 510
EcoRV GATATC 1 cut(s) 801
EcoT14I CCWWGG 2 cut(s) 249, 261
EcoT22I ATGCAT 1 cut(s) 595
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 2 cut(s) 249, 261
FaeI CATG 1 cut(s) 937
FalI AAGNNNNNCTT 2 cut(s) 887, 919
FaqI GGGAC 2 cut(s) 1175, 1214
FatI CATG 1 cut(s) 933
FauI CCCGC 2 cut(s) 248, 511
Fnu4HI GCNGC 3 cut(s) 255, 376, 379
FokI GGATG 5 cut(s) 47, 527, 602, 733, 909
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 3 cut(s) 255, 376, 379
GluI GCNGC 3 cut(s) 255, 376, 379
GsaI CCCAGC 2 cut(s) 95, 510
GsuI CTGGAG 1 cut(s) 1191
HaeIII GGCC 3 cut(s) 254, 661, 791
HapII CCGG 1 cut(s) 1198
Hin1II CATG 1 cut(s) 937
HindIII AAGCTT 2 cut(s) 281, 416
HinfI GANTC 9 cut(s) 98, 134, 400, 409, 502, 737, 743, 998, 1163
HpaII CCGG 1 cut(s) 1198
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 2 cut(s) 681, 769
Hpy188III TCNNGA 4 cut(s) 404, 872, 926, 1208
Hpy8I GTNNAC 2 cut(s) 681, 769
HpyAV CCTTC 5 cut(s) 255, 448, 556, 667, 893
HpyCH4IV ACGT 2 cut(s) 369, 771
HpyCH4V TGCA 5 cut(s) 314, 593, 944, 1016, 1117
HpyF10VI GCNNNNNNNGC 1 cut(s) 424
HpyF3I CTNAG 8 cut(s) 17, 85, 179, 216, 647, 741, 873, 1044
HpySE526I ACGT 2 cut(s) 369, 771
Hsp92II CATG 1 cut(s) 937
Kzo9I GATC 1 cut(s) 1047
LmnI GCTCC 1 cut(s) 533
Lsp1109I GCAGC 1 cut(s) 387
LweI GCATC 2 cut(s) 505, 580
MaeII ACGT 2 cut(s) 369, 771
MalI GATC 1 cut(s) 1049
MbiI CCGCTC 1 cut(s) 442
MboI GATC 1 cut(s) 1047
MhlI GDGCHC 2 cut(s) 538, 587
MluCI AATT 4 cut(s) 544, 957, 983, 1243
MlyI GAGTC 7 cut(s) 92, 128, 418, 746, 752, 1007, 1157
MmeI TCCRAC 3 cut(s) 37, 156, 174
Mph1103I ATGCAT 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 917
MseI TTAA 4 cut(s) 987, 1095, 1136, 1246
MspCI CTTAAG 1 cut(s) 1094
MspI CCGG 1 cut(s) 1198
MspR9I CCNGG 2 cut(s) 512, 1199
Mva1269I GAATGC 1 cut(s) 1087
MvaI CCWGG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 424
NciI CCSGG 1 cut(s) 1199
NdeII GATC 1 cut(s) 1047
NlaIII CATG 1 cut(s) 937
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 937
NspV TTCGAA 2 cut(s) 301, 1147
PceI AGGCCT 1 cut(s) 791
PciI ACATGT 1 cut(s) 933
PctI GAATGC 1 cut(s) 1087
PdmI GAANNNNTTC 1 cut(s) 917
PfeI GAWTC 2 cut(s) 400, 502
PfoI TCCNGGA 1 cut(s) 1197
PkrI GCNGC 3 cut(s) 256, 377, 380
PleI GAGTC 7 cut(s) 92, 128, 417, 745, 751, 1006, 1157
PpsI GAGTC 7 cut(s) 92, 128, 417, 745, 751, 1006, 1157
PscI ACATGT 1 cut(s) 933
PsiI TTATAA 2 cut(s) 422, 555
Psp124BI GAGCTC 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 510
PspFI CCCAGC 2 cut(s) 91, 506
PspGI CCWGG 1 cut(s) 510
PspPI GGNCC 1 cut(s) 660
PstI CTGCAG 1 cut(s) 946
RsaI GTAC 1 cut(s) 1226
RsaNI GTAC 1 cut(s) 1225
SacI GAGCTC 1 cut(s) 587
SaqAI TTAA 4 cut(s) 987, 1095, 1136, 1246
SatI GCNGC 3 cut(s) 255, 376, 379
Sau3AI GATC 1 cut(s) 1047
Sau96I GGNCC 1 cut(s) 660
SchI GAGTC 7 cut(s) 92, 128, 418, 746, 752, 1007, 1157
ScrFI CCNGG 2 cut(s) 512, 1199
SduI GDGCHC 2 cut(s) 538, 587
SfaNI GCATC 2 cut(s) 505, 580
SfcI CTRYAG 1 cut(s) 942
SfuI TTCGAA 2 cut(s) 301, 1147
SmlI CTYRAG 1 cut(s) 1094
SmoI CTYRAG 1 cut(s) 1094
Sse9I AATT 4 cut(s) 544, 957, 983, 1243
SseBI AGGCCT 1 cut(s) 791
SsiI CCGC 6 cut(s) 241, 255, 287, 379, 442, 518
SstI GAGCTC 1 cut(s) 587
StuI AGGCCT 1 cut(s) 791
StyD4I CCNGG 2 cut(s) 510, 1197
StyI CCWWGG 2 cut(s) 249, 261
TaiI ACGT 2 cut(s) 372, 774
TaqI TCGA 3 cut(s) 301, 833, 1147
TaqII GACCGA 1 cut(s) 617
TasI AATT 4 cut(s) 544, 957, 983, 1243
TatI WGTACW 1 cut(s) 1224
TauI GCSGC 2 cut(s) 257, 381
TfiI GAWTC 2 cut(s) 400, 502
Tru1I TTAA 4 cut(s) 987, 1095, 1136, 1246
Tru9I TTAA 4 cut(s) 987, 1095, 1136, 1246
TscAI CASTG 1 cut(s) 316
TseI GCWGC 1 cut(s) 375
TspDTI ATGAA 3 cut(s) 515, 899, 996
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 1 cut(s) 316
Vha464I CTTAAG 1 cut(s) 1094
XapI RAATTY 2 cut(s) 544, 983
XceI RCATGY 1 cut(s) 937
XmnI GAANNNNTTC 1 cut(s) 917
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.