pycom12419g00120

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012419
OG0001441
Physical Location & Seq
Reverse (-)
178501 .. 179194
694 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12419g00120.2

Sequence Viewer

Length: 642 bp
ATGTTAGATTTCAATTTTTTTAATATCCAATGGTCCAAATTAATTAAGTTAATAAAAATTCAAAAAAAAAAATCAAATTTTTTAAAATTATATAATTTTTATAAATACCTTACTATTATCTTTCACCTTACACCACATTTCAATATTTCTCTCAAATTTCCATCTGACCAAAAGATCGATCCTTTTGACCCGAAGGAACCCACGGACCCAAACCCCCATTCTCACTCTTCCCAGCAACAATCGCCGCCGCCGCCGGAACAGAGTAGACCCACTCCTCAAAATCCGTCATGTACAAACCCTTCCCCATCCGGGACCTCCAAAGTTGCTTCCTTGGAAGAAATCGGCAAAAACCTCTCCGAGTTTCGGCGCGGATCCTCCGTGCCGGACCCCACAGAATCAGGCCCTGCATTTTCGCAACATTCTTCTACGGCTTCAGGTTTCCCACAGCAAATCTCTTTTCAAGAGCTCTACAAGAGAAATGTGCTTGGCAAGGCCGAGGGCGGCAATGCCGATAGTGACCGGAAGCTCTCTTTTGATACGATTCGAGAGAGTTTGAGGAAGATAAATAATCCCAGTGTGCAAAATGATAGAAGGACCCTTCGTCATTGTCGCAGTATACGGAGAGTTTGCGCCAGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

214

Amino Acids

24.25

Weight (kDa)

9.82

Isoelectric Point (pI)

65.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AccI GTMKAC 2 cut(s) 265, 616
AccII CGCG 1 cut(s) 369
AciI CCGC 5 cut(s) 245, 248, 251, 369, 501
AclWI GGATC 3 cut(s) 173, 366, 379
AcsI RAATTY 3 cut(s) 57, 76, 155
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 2 cut(s) 309, 363
AgsI TTSAA 4 cut(s) 13, 62, 142, 461
AhdI GACNNNNNGTC 1 cut(s) 600
AjuI GAANNNNNNNTTGG 2 cut(s) 202, 234
AluBI AGCT 3 cut(s) 466, 526, 639
AluI AGCT 3 cut(s) 466, 526, 639
Alw21I GWGCWC 1 cut(s) 468
AlwI GGATC 3 cut(s) 173, 366, 379
AlwNI CAGNNNCTG 2 cut(s) 404, 639
AoxI GGCC 2 cut(s) 400, 492
ApoI RAATTY 3 cut(s) 57, 76, 155
AseI ATTAAT 1 cut(s) 41
AspLEI GCGC 2 cut(s) 369, 632
AspS9I GGNCC 6 cut(s) 33, 205, 312, 385, 401, 594
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 116
AvaII GGWCC 5 cut(s) 33, 205, 312, 385, 594
BamHI GGATCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 468
BarI GAAGNNNNNNTAC 2 cut(s) 283, 315
Bbv12I GWGCWC 1 cut(s) 468
BccI CCATC 2 cut(s) 169, 313
BceAI ACGGC 1 cut(s) 444
BcnI CCSGG 1 cut(s) 310
BisI GCNGC 4 cut(s) 245, 248, 251, 502
BlsI GCNGC 4 cut(s) 246, 249, 252, 503
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 5 cut(s) 33, 205, 312, 385, 594
BmeRI GACNNNNNGTC 1 cut(s) 600
BmgT120I GGNCC 6 cut(s) 33, 205, 312, 385, 401, 594
BmiI GGNNCC 6 cut(s) 198, 207, 313, 373, 387, 596
BmrFI CCNGG 1 cut(s) 310
BmrI ACTGGG 1 cut(s) 567
BmuI ACTGGG 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 310
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 3 cut(s) 201, 330, 495
BsaWI WCCGGW 1 cut(s) 519
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 363
Bse1I ACTGG 1 cut(s) 573
Bse3DI GCAATG 1 cut(s) 511
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 201, 330, 495
BseGI GGATG 1 cut(s) 305
BseLI CCNNNNNNNGG 2 cut(s) 309, 363
BseMI GCAATG 1 cut(s) 511
BseNI ACTGG 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 264
BseYI CCCAGC 1 cut(s) 231
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 2 cut(s) 402, 494
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 468
BsiSI CCGG 4 cut(s) 254, 309, 383, 520
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 2 cut(s) 309, 363
BsmFI GGGAC 1 cut(s) 325
BsnI GGCC 2 cut(s) 402, 494
Bsp1286I GDGCHC 1 cut(s) 468
Bsp1407I TGTACA 1 cut(s) 290
Bsp143I GATC 3 cut(s) 174, 178, 371
BspACI CCGC 5 cut(s) 245, 248, 251, 369, 501
BspANI GGCC 2 cut(s) 402, 494
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 6 cut(s) 198, 207, 313, 373, 387, 596
BspPI GGATC 3 cut(s) 173, 366, 379
BsrDI GCAATG 1 cut(s) 511
BsrGI TGTACA 1 cut(s) 290
BsrI ACTGG 1 cut(s) 573
BssECI CCNNGG 3 cut(s) 201, 330, 495
BssMI GATC 3 cut(s) 174, 178, 371
BssNAI GTATAC 1 cut(s) 617
BssT1I CCWWGG 1 cut(s) 330
Bst1107I GTATAC 1 cut(s) 617
Bst6I CTCTTC 1 cut(s) 232
BstAUI TGTACA 1 cut(s) 290
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 305
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 2 cut(s) 369, 632
BstKTI GATC 3 cut(s) 177, 181, 374
BstMBI GATC 3 cut(s) 174, 178, 371
BstMWI GCNNNNNNNGC 3 cut(s) 241, 250, 636
BstSCI CCNGG 1 cut(s) 308
BstUI CGCG 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 371
BstYI RGATCY 1 cut(s) 371
BstZ17I GTATAC 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 2 cut(s) 402, 494
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 305
BtsIMutI CAGTG 1 cut(s) 580
CaiI CAGNNNCTG 2 cut(s) 404, 639
CfoI GCGC 2 cut(s) 369, 632
Cfr13I GGNCC 6 cut(s) 33, 205, 312, 385, 401, 594
ClaI ATCGAT 1 cut(s) 177
Csp6I GTAC 1 cut(s) 291
CviAII CATG 1 cut(s) 288
CviJI RGCY 6 cut(s) 402, 431, 466, 494, 526, 639
CviKI_1 RGCY 6 cut(s) 402, 431, 466, 494, 526, 639
CviQI GTAC 1 cut(s) 291
DpnI GATC 3 cut(s) 176, 180, 373
DpnII GATC 3 cut(s) 174, 178, 371
DraI TTTAAA 1 cut(s) 84
DriI GACNNNNNGTC 1 cut(s) 600
Eam1104I CTCTTC 1 cut(s) 232
Eam1105I GACNNNNNGTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 330
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 5 cut(s) 33, 205, 312, 385, 594
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 466
EcoO109I RGGNCCY 3 cut(s) 312, 401, 594
EcoT14I CCWWGG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 1 cut(s) 291
FaiI YATR 5 cut(s) 91, 93, 102, 289, 617
FaqI GGGAC 1 cut(s) 325
FatI CATG 1 cut(s) 287
FblI GTMKAC 2 cut(s) 265, 616
Fnu4HI GCNGC 4 cut(s) 245, 248, 251, 502
FokI GGATG 1 cut(s) 292
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 4 cut(s) 245, 248, 251, 502
GlaI GCGC 2 cut(s) 368, 631
GluI GCNGC 4 cut(s) 245, 248, 251, 502
GsaI CCCAGC 1 cut(s) 235
HaeIII GGCC 2 cut(s) 402, 494
HapII CCGG 4 cut(s) 254, 309, 383, 520
HhaI GCGC 2 cut(s) 369, 632
Hin1II CATG 1 cut(s) 291
Hin6I GCGC 2 cut(s) 367, 630
HinP1I GCGC 2 cut(s) 367, 630
HinfI GANTC 2 cut(s) 395, 541
HpaII CCGG 4 cut(s) 254, 309, 383, 520
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 2 cut(s) 266, 617
Hpy188I TCNGA 2 cut(s) 166, 358
Hpy188III TCNNGA 2 cut(s) 461, 545
Hpy8I GTNNAC 2 cut(s) 266, 617
HpyAV CCTTC 4 cut(s) 187, 309, 585, 608
HpyCH4V TGCA 2 cut(s) 407, 580
HpyF10VI GCNNNNNNNGC 3 cut(s) 241, 250, 636
Hsp92II CATG 1 cut(s) 291
HspAI GCGC 2 cut(s) 367, 630
Kzo9I GATC 3 cut(s) 174, 178, 371
LpnPI CCDG 9 cut(s) 245, 267, 322, 384, 396, 417, 420, 533, 586
MaeIII GTNAC 1 cut(s) 515
MalI GATC 3 cut(s) 176, 180, 373
MboI GATC 3 cut(s) 174, 178, 371
MboII GAAGA 4 cut(s) 219, 347, 414, 571
MflI RGATCY 1 cut(s) 371
MhlI GDGCHC 1 cut(s) 468
MluCI AATT 8 cut(s) 13, 38, 42, 57, 76, 86, 94, 155
MnlI CCTC 6 cut(s) 285, 325, 362, 385, 490, 549
MseI TTAA 5 cut(s) 21, 41, 45, 50, 83
MspI CCGG 4 cut(s) 254, 309, 383, 520
MspR9I CCNGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 3 cut(s) 241, 250, 636
NciI CCSGG 1 cut(s) 310
NdeII GATC 3 cut(s) 174, 178, 371
NlaIII CATG 1 cut(s) 291
NlaIV GGNNCC 6 cut(s) 198, 207, 313, 373, 387, 596
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 1 cut(s) 515
PacI TTAATTAA 1 cut(s) 45
PfeI GAWTC 2 cut(s) 395, 541
PfoI TCCNGGA 1 cut(s) 308
PkrI GCNGC 4 cut(s) 246, 249, 252, 503
PpuMI RGGWCCY 2 cut(s) 312, 594
PshBI ATTAAT 1 cut(s) 41
PsiI TTATAA 1 cut(s) 102
Psp124BI GAGCTC 1 cut(s) 468
Psp5II RGGWCCY 2 cut(s) 312, 594
PspFI CCCAGC 1 cut(s) 231
PspN4I GGNNCC 6 cut(s) 198, 207, 313, 373, 387, 596
PspPI GGNCC 6 cut(s) 33, 205, 312, 385, 401, 594
PspPPI RGGWCCY 2 cut(s) 312, 594
PstNI CAGNNNCTG 2 cut(s) 404, 639
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SacI GAGCTC 1 cut(s) 468
SaqAI TTAA 5 cut(s) 21, 41, 45, 50, 83
SatI GCNGC 4 cut(s) 245, 248, 251, 502
Sau3AI GATC 3 cut(s) 174, 178, 371
Sau96I GGNCC 6 cut(s) 33, 205, 312, 385, 401, 594
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 1 cut(s) 468
SetI ASST 8 cut(s) 111, 129, 317, 354, 439, 468, 528, 641
SinI GGWCC 5 cut(s) 33, 205, 312, 385, 594
Sse9I AATT 8 cut(s) 13, 38, 42, 57, 76, 86, 94, 155
SsiI CCGC 5 cut(s) 245, 248, 251, 369, 501
SspI AATATT 1 cut(s) 145
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 308
StyI CCWWGG 1 cut(s) 330
TaqI TCGA 2 cut(s) 177, 544
TasI AATT 8 cut(s) 13, 38, 42, 57, 76, 86, 94, 155
TatI WGTACW 1 cut(s) 290
TauI GCSGC 4 cut(s) 247, 250, 253, 504
TfiI GAWTC 2 cut(s) 395, 541
Tru1I TTAA 5 cut(s) 21, 41, 45, 50, 83
Tru9I TTAA 5 cut(s) 21, 41, 45, 50, 83
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 515
TspGWI ACGGA 4 cut(s) 218, 273, 367, 634
TspRI CASTG 1 cut(s) 580
VpaK11BI GGWCC 5 cut(s) 33, 205, 312, 385, 594
VspI ATTAAT 1 cut(s) 41
XapI RAATTY 3 cut(s) 57, 76, 155
XmiI GTMKAC 2 cut(s) 265, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.