Rroxscaffold_5G00352280

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001441
Physical Location & Seq
Reverse (-)
29280802 .. 29285180
4379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352280.1

Sequence Viewer

Length: 1254 bp
ATGATGGCTCTCCGACTGAGAACCAACCACCACCGAACCCTCACAAACCCATCATCTCTCATTCACCTCTTCTCCACTTCCTCCGCAGACCCATCCGACTCAACAGACCCAACTGAACCCAATCCCCAGTCCGACTCTTCCTCTCTCTCCAACTACTTCAGCGATGTCAAAGCCAGCCTCAGGCAACAGCAACAACACAAGAGCAGACCCACTTCTCAGAACAACAGCCCTTCTCTCTCCCGCCCTTCCAAGACCGCCTCCTTCGAGGAAATCCGCAACAAGCTTGCGGAGTTTCGGCTTCGATCCATTGGGCCAAACCCCACTGCACCTCCACCTCCGAACCCCATTTCGTTTGAGAAGCTCTATAACAAAGACGTGAGCAGCCGCAAGATGGAGTTTCAGTCGATTCGGGAGAGTCTGAAGCTTATAAAGCCCAATGCCGATAAGCAGAGTGAGAGGAAGGGTGAGGGTTTGCCGTTGTTGAGGTTTCCGAAAAGTTTGGATATGAATCCCAGCCAGGATGCGGGTGTTAGGGAGGGAGCACTTCCGAGTTTGTTTTATAAGGAGAAGGAGAGAAGCGGAGACAAGAGCTCGGATGCATTGAAGACCGAGTTTGTGAAGATTTATAGCTATAAAGAGTTGGGGGAGAAGCTGAGCGAGTTGAGGCCCGAGGAGGGGAAGGAGTTTACATTGGGGGAGTTGAGTGAGAGGTTGGTCAAGTTGAGGATGATGGAAGAGAATGAGTCTGAGTCTCGGGTTGGGGAAGTGTTTGTGGACTTGAGGCACAGCTTGGAGGCCTTGAAGATATCCCAACAAGAGGAGAAGCATAGGAAAACTTCGAGGCAGAGGCTTCGTATCATAGATATAGGTCAAACTCCTGAGTATATGATGAAACCCCCAAAGGAAGGCTTGGTTGAGAAGTATTTCCATCCAGATAACATGTCTTCTGCAGAAAAGCTGAAAATTGAGCTTGGCAAAGTTAGAGATGAATTTAAAATGTCAGAGTCAGATTGTGGTTCTGCACGAGTTCAAGTGGCACAACTCACAACTAAGATCAAACATCTGTCTTCAGTTTTACACAAAAAGGATAAGCATTCACTTAAGGGTCTTCAAGCAATGGTGCAGAGGAGAAAGAAGCTATTAAAGTATCTTCGAAGAACTGACTGGGACTCTTACTGCCTTGTTCTCTCCAAACTTGGTCTCCGGGACACGCCAGATGTCAAACAAGAGTACAAGAAACATTACAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

417

Amino Acids

47.9

Weight (kDa)

9.58

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S15 PF00312 327 - 402 1.7e-23 Ribosomal protein S15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 428, 561
AciI CCGC 8 cut(s) 84, 241, 255, 274, 287, 385, 524, 579
AclWI GGATC 1 cut(s) 297
AcsI RAATTY 1 cut(s) 989
AcuI CTGAAG 3 cut(s) 142, 440, 1053
AfaI GTAC 1 cut(s) 1232
AfiI CCNNNNNNNGG 7 cut(s) 180, 391, 523, 674, 675, 817, 905
AflII CTTAAG 1 cut(s) 1100
AflIII ACRYGT 1 cut(s) 939
AgsI TTSAA 4 cut(s) 604, 802, 1031, 1112
AjiI CACGTC 1 cut(s) 376
AjnI CCWGG 1 cut(s) 516
Alw21I GWGCWC 2 cut(s) 544, 593
Alw26I GTCTC 3 cut(s) 576, 756, 1205
AlwI GGATC 1 cut(s) 297
Ama87I CYCGRG 2 cut(s) 668, 753
AoxI GGCC 3 cut(s) 311, 665, 795
ApeKI GCWGC 1 cut(s) 381
ApoI RAATTY 1 cut(s) 989
Asp700I GAANNNNTTC 1 cut(s) 923
AspS9I GGNCC 2 cut(s) 311, 666
AsuC2I CCSGG 1 cut(s) 1205
AsuHPI GGTGA 2 cut(s) 56, 476
AsuII TTCGAA 1 cut(s) 1153
AvaI CYCGRG 2 cut(s) 668, 753
AxyI CCTNAGG 1 cut(s) 179
BanII GRGCYC 1 cut(s) 593
BauI CACGAG 1 cut(s) 1023
BbsI GAAGAC 4 cut(s) 611, 936, 1059, 1100
Bbv12I GWGCWC 2 cut(s) 544, 593
BbvI GCAGC 1 cut(s) 393
BccI CCATC 5 cut(s) 58, 100, 385, 724, 936
BceAI ACGGC 1 cut(s) 460
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 1 cut(s) 518
BcnI CCSGG 1 cut(s) 1205
BcoDI GTCTC 3 cut(s) 576, 756, 1205
BfmI CTRYAG 1 cut(s) 948
BfrI CTTAAG 1 cut(s) 1100
BisI GCNGC 2 cut(s) 382, 385
BlpI GCTNAGC 1 cut(s) 653
BlsI GCNGC 2 cut(s) 383, 386
Bme1390I CCNGG 2 cut(s) 518, 1205
BmeT110I CYCGRG 2 cut(s) 668, 753
BmgBI CACGTC 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 311, 666
BmrFI CCNGG 2 cut(s) 518, 1205
BmrI ACTGGG 2 cut(s) 121, 1174
BmsI GCATC 2 cut(s) 511, 586
BmuI ACTGGG 2 cut(s) 121, 1174
BpiI GAAGAC 4 cut(s) 611, 936, 1059, 1100
Bpu1102I GCTNAGC 1 cut(s) 653
Bpu14I TTCGAA 1 cut(s) 1153
BpuEI CTTGAG 1 cut(s) 799
BpuMI CCSGG 1 cut(s) 1205
BsaBI GATNNNNATC 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 1205
BsaJI CCNNGG 1 cut(s) 669
BsaXI ACNNNNNCTCC 8 cut(s) 56, 86, 313, 343, 386, 416, 1185, 1215
Bsc4I CCNNNNNNNGG 7 cut(s) 180, 391, 523, 674, 675, 817, 905
Bse1I ACTGG 2 cut(s) 127, 1169
Bse21I CCTNAGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 1122
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 1 cut(s) 669
BseGI GGATG 5 cut(s) 92, 526, 601, 732, 928
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 7 cut(s) 180, 391, 523, 674, 675, 817, 905
BseMI GCAATG 1 cut(s) 1122
BseMII CTCAG 6 cut(s) 8, 193, 230, 644, 738, 870
BseNI ACTGG 2 cut(s) 127, 1169
BseRI GAGGAG 3 cut(s) 686, 833, 1141
BseXI GCAGC 1 cut(s) 393
BseYI CCCAGC 1 cut(s) 512
BsgI GTGCAG 3 cut(s) 309, 1005, 1142
BshFI GGCC 3 cut(s) 313, 667, 797
BsiHKAI GWGCWC 2 cut(s) 544, 593
BsiHKCI CYCGRG 2 cut(s) 668, 753
BsiSI CCGG 1 cut(s) 1204
BslFI GGGAC 2 cut(s) 1181, 1220
BslI CCNNNNNNNGG 7 cut(s) 180, 391, 523, 674, 675, 817, 905
BsmAI GTCTC 3 cut(s) 576, 756, 1205
BsmFI GGGAC 2 cut(s) 1181, 1220
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 3 cut(s) 313, 667, 797
Bso31I GGTCTC 1 cut(s) 1205
BsoBI CYCGRG 2 cut(s) 668, 753
Bsp119I TTCGAA 1 cut(s) 1153
Bsp1286I GDGCHC 2 cut(s) 544, 593
Bsp143I GATC 2 cut(s) 302, 1053
Bsp1720I GCTNAGC 1 cut(s) 653
BspACI CCGC 8 cut(s) 84, 241, 255, 274, 287, 385, 524, 579
BspANI GGCC 3 cut(s) 313, 667, 797
BspCNI CTCAG 6 cut(s) 9, 192, 229, 645, 739, 871
BspMAI CTGCAG 1 cut(s) 952
BspPI GGATC 1 cut(s) 297
BspT104I TTCGAA 1 cut(s) 1153
BspTI CTTAAG 1 cut(s) 1100
BspTNI GGTCTC 1 cut(s) 1205
BsrDI GCAATG 1 cut(s) 1122
BsrI ACTGG 2 cut(s) 127, 1169
BssECI CCNNGG 1 cut(s) 669
BssMI GATC 2 cut(s) 302, 1053
BssSI CACGAG 1 cut(s) 1023
Bst2BI CACGAG 1 cut(s) 1023
Bst2UI CCWGG 1 cut(s) 518
Bst6I CTCTTC 3 cut(s) 74, 142, 729
BstAFI CTTAAG 1 cut(s) 1100
BstBI TTCGAA 1 cut(s) 1153
BstC8I GCNNGC 2 cut(s) 175, 285
BstDEI CTNAG 7 cut(s) 17, 179, 216, 653, 747, 879, 1050
BstF5I GGATG 5 cut(s) 92, 526, 601, 732, 928
BstKTI GATC 2 cut(s) 305, 1056
BstMAI GTCTC 3 cut(s) 576, 756, 1205
BstMBI GATC 2 cut(s) 302, 1053
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNI CCWGG 1 cut(s) 518
BstNSI RCATGY 1 cut(s) 943
BstSCI CCNGG 2 cut(s) 516, 1203
BstSFI CTRYAG 1 cut(s) 948
BstV1I GCAGC 1 cut(s) 393
BstV2I GAAGAC 4 cut(s) 611, 936, 1059, 1100
Bsu36I CCTNAGG 1 cut(s) 179
BsuRI GGCC 3 cut(s) 313, 667, 797
BtgZI GCGATG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 376
BtsCI GGATG 5 cut(s) 92, 526, 601, 732, 928
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 2 cut(s) 175, 285
Cfr13I GGNCC 2 cut(s) 311, 666
Csp6I GTAC 1 cut(s) 1231
CviAII CATG 1 cut(s) 940
CviQI GTAC 1 cut(s) 1231
DdeI CTNAG 7 cut(s) 17, 179, 216, 653, 747, 879, 1050
DpnI GATC 2 cut(s) 304, 1055
DpnII GATC 2 cut(s) 302, 1053
DraI TTTAAA 1 cut(s) 994
Eam1104I CTCTTC 3 cut(s) 74, 142, 729
EarI CTCTTC 3 cut(s) 74, 142, 729
Ecl136II GAGCTC 1 cut(s) 591
Eco147I AGGCCT 1 cut(s) 797
Eco24I GRGCYC 1 cut(s) 593
Eco31I GGTCTC 1 cut(s) 1205
Eco32I GATATC 1 cut(s) 807
Eco53kI GAGCTC 1 cut(s) 591
Eco57I CTGAAG 3 cut(s) 142, 440, 1053
Eco81I CCTNAGG 1 cut(s) 179
Eco88I CYCGRG 2 cut(s) 668, 753
EcoICRI GAGCTC 1 cut(s) 591
EcoRII CCWGG 1 cut(s) 516
EcoRV GATATC 1 cut(s) 807
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 593
FaeI CATG 1 cut(s) 943
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FaqI GGGAC 2 cut(s) 1181, 1220
FatI CATG 1 cut(s) 939
FauI CCCGC 2 cut(s) 248, 517
Fnu4HI GCNGC 2 cut(s) 382, 385
FokI GGATG 5 cut(s) 79, 533, 608, 739, 915
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 2 cut(s) 382, 385
GluI GCNGC 2 cut(s) 382, 385
GsaI CCCAGC 1 cut(s) 516
HaeIII GGCC 3 cut(s) 313, 667, 797
HapII CCGG 1 cut(s) 1204
Hin1II CATG 1 cut(s) 943
HindIII AAGCTT 2 cut(s) 281, 422
HinfI GANTC 9 cut(s) 98, 134, 406, 415, 508, 743, 749, 1004, 1169
HpaII CCGG 1 cut(s) 1204
HphI GGTGA 2 cut(s) 56, 476
Hpy166II GTNNAC 2 cut(s) 687, 775
Hpy188III TCNNGA 3 cut(s) 410, 878, 932
Hpy8I GTNNAC 2 cut(s) 687, 775
HpyAV CCTTC 7 cut(s) 240, 255, 271, 454, 562, 673, 899
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 5 cut(s) 326, 599, 950, 1022, 1123
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 7 cut(s) 17, 179, 216, 653, 747, 879, 1050
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 1 cut(s) 943
Kzo9I GATC 2 cut(s) 302, 1053
LmnI GCTCC 1 cut(s) 539
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 2 cut(s) 511, 586
MaeII ACGT 1 cut(s) 375
MalI GATC 2 cut(s) 304, 1055
MboI GATC 2 cut(s) 302, 1053
MhlI GDGCHC 2 cut(s) 544, 593
MluCI AATT 3 cut(s) 963, 989, 1249
MlyI GAGTC 7 cut(s) 92, 128, 424, 752, 758, 1013, 1163
MmeI TCCRAC 4 cut(s) 37, 120, 156, 174
Mph1103I ATGCAT 1 cut(s) 601
MroXI GAANNNNTTC 1 cut(s) 923
MseI TTAA 4 cut(s) 993, 1101, 1142, 1252
MspCI CTTAAG 1 cut(s) 1100
MspI CCGG 1 cut(s) 1204
MspR9I CCNGG 2 cut(s) 518, 1205
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 1 cut(s) 518
MwoI GCNNNNNNNGC 1 cut(s) 430
NciI CCSGG 1 cut(s) 1205
NdeII GATC 2 cut(s) 302, 1053
NlaIII CATG 1 cut(s) 943
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 1 cut(s) 943
NspV TTCGAA 1 cut(s) 1153
PceI AGGCCT 1 cut(s) 797
PciI ACATGT 1 cut(s) 939
PctI GAATGC 1 cut(s) 1093
PdmI GAANNNNTTC 1 cut(s) 923
PfeI GAWTC 2 cut(s) 406, 508
PfoI TCCNGGA 1 cut(s) 1203
PkrI GCNGC 2 cut(s) 383, 386
PleI GAGTC 7 cut(s) 92, 128, 423, 751, 757, 1012, 1163
PpsI GAGTC 7 cut(s) 92, 128, 423, 751, 757, 1012, 1163
PscI ACATGT 1 cut(s) 939
PsiI TTATAA 2 cut(s) 428, 561
Psp124BI GAGCTC 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 516
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 1 cut(s) 516
PspPI GGNCC 2 cut(s) 311, 666
PstI CTGCAG 1 cut(s) 952
RsaI GTAC 1 cut(s) 1232
RsaNI GTAC 1 cut(s) 1231
SacI GAGCTC 1 cut(s) 593
SaqAI TTAA 4 cut(s) 993, 1101, 1142, 1252
SatI GCNGC 2 cut(s) 382, 385
Sau3AI GATC 2 cut(s) 302, 1053
Sau96I GGNCC 2 cut(s) 311, 666
SchI GAGTC 7 cut(s) 92, 128, 424, 752, 758, 1013, 1163
ScrFI CCNGG 2 cut(s) 518, 1205
SduI GDGCHC 2 cut(s) 544, 593
SfaNI GCATC 2 cut(s) 511, 586
SfcI CTRYAG 1 cut(s) 948
SfuI TTCGAA 1 cut(s) 1153
SmlI CTYRAG 2 cut(s) 778, 1100
SmoI CTYRAG 2 cut(s) 778, 1100
Sse9I AATT 3 cut(s) 963, 989, 1249
SseBI AGGCCT 1 cut(s) 797
SsiI CCGC 8 cut(s) 84, 241, 255, 274, 287, 385, 524, 579
SstI GAGCTC 1 cut(s) 593
StuI AGGCCT 1 cut(s) 797
StyD4I CCNGG 2 cut(s) 516, 1203
TaiI ACGT 1 cut(s) 378
TaqI TCGA 5 cut(s) 264, 301, 404, 839, 1153
TaqII GACCGA 1 cut(s) 623
TasI AATT 3 cut(s) 963, 989, 1249
TatI WGTACW 1 cut(s) 1230
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 2 cut(s) 406, 508
Tru1I TTAA 4 cut(s) 993, 1101, 1142, 1252
Tru9I TTAA 4 cut(s) 993, 1101, 1142, 1252
TscAI CASTG 1 cut(s) 328
TseI GCWGC 1 cut(s) 381
TspDTI ATGAA 3 cut(s) 521, 905, 1002
TspRI CASTG 1 cut(s) 328
Vha464I CTTAAG 1 cut(s) 1100
XapI RAATTY 1 cut(s) 989
XceI RCATGY 1 cut(s) 943
XmnI GAANNNNTTC 1 cut(s) 923
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.