RchiOBHm_Chr4g0407691

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001441
Physical Location & Seq
Reverse (-)
30032564 .. 30037394
4831 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37891

Sequence Viewer

Length: 1260 bp
ATGATGGCTCTCCGACTGAGAACCAACCAGCACCGAACCCTCACAAACCCATCATCTCTCATCCACCTCTTCTCCACTTCCTCCTCAGACCCATCCGACTCAACTGACCCAACTGAACCCAATCCCCAGTCCGACTCTTCCTCTCTCTCCAACTACTTCAGCGATGTCAAAGCCAGCCTCAGGCAACAGCAACAACACAGGAGCAGACCCACTTCTCAGAACAACAGCAACAACCCTTCTCTCTCCCGCCCTTCCAAGGCCACCTCCTTGGAGGAAATCAGCAACAAGCTTGCGGAGTTTCGGCTTGGATCCATTGGGCCAAACCCCACTGCACCTCCACCTCCGAACCCCATTTCGTTTGAGAAGCTCTATAACAAAGACGTGAGCAGCCGCAAGATGGAGTTTCAGACGATTCGGGAGAGTCTGAAGCTTATAAAGCCCAATGCCGATGAGCGGAATGAGAGGAAGGGTGAGGGTTTGCCGAAGTTGAGGTTTCGGAAAAGTTTGGATATGAATCCCAGCCAGGATGCGGGTGTTAGGGAGGGAGCACTTCCGAGTTTGTTTTATAAGGAGAAGGAGAGAAGCGGAGACAAGAGCTCGGATGCATTGAAGACCGAGTTTGTGAAGATTTATAGCTATAAAGAGTTGGGGGAGAAGCTGAGCGAGTTGAGGCCTGAGGAGGGGAAGGAGTTTACATTGGGGGAGTTGAGTGAGAGGTTGGTCAAGTTGAGGATGATGGAAGAGAAAGAGTCTGAGTCTCGGGTTGGGGGAGTGTTTGTGGACTTGAGGCACACCTTGGAGGCCTTGAAGATATCCCAACAAGAGGAGAAGCATAGGAAAACTTCGAGGCAGAGGCTTCGTATCATAGATATAGGTCAAACTCCTGAGTATATGATGAAACCCCCAAAGGAAGGCTTGGTTGAGAAGTATTTCCATCCAGATAACATGTCTTCTGCAGAAAAGCTGAAAATTGAGCTTGGCAAAGTTAGAGATGAATTTAAAATGTCAGAGTCAGATTGTGGTTCTGCACGAGTTCAAGTGGCACAACTCACAACTAAGATCAAACATCTATCTTCAGTTTTACACAAAAAGGATAAGCATTCACTTAAGGGTCTTCAAGCAATGGTGCAGAGAAGAAAGAAGCTATTAAAGTATCTTCGAAGAACTGACTGGGACTCTTACTGCCTTGTTCTCTCCAAACTTGGTCTCCGGGACACGCCAGATGTCAAACAAGAGTACAAGAAACATTACAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

419

Amino Acids

48.07

Weight (kDa)

9.61

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S15 PF00312 329 - 404 1.7e-23 Ribosomal protein S15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 434, 567
AccBSI CCGCTC 1 cut(s) 454
AciI CCGC 6 cut(s) 247, 293, 391, 454, 530, 585
AclWI GGATC 2 cut(s) 303, 316
AcsI RAATTY 1 cut(s) 995
AcuI CTGAAG 3 cut(s) 142, 446, 1059
AfaI GTAC 1 cut(s) 1238
AfiI CCNNNNNNNGG 8 cut(s) 180, 256, 397, 453, 529, 680, 823, 911
AflII CTTAAG 1 cut(s) 1106
AflIII ACRYGT 1 cut(s) 945
AgsI TTSAA 4 cut(s) 610, 808, 1037, 1118
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 1 cut(s) 522
AluBI AGCT 9 cut(s) 289, 367, 430, 597, 636, 658, 964, 976, 1144
AluI AGCT 9 cut(s) 289, 367, 430, 597, 636, 658, 964, 976, 1144
Alw21I GWGCWC 2 cut(s) 550, 599
Alw26I GTCTC 3 cut(s) 582, 762, 1211
AlwI GGATC 2 cut(s) 303, 316
Ama87I CYCGRG 1 cut(s) 759
AoxI GGCC 4 cut(s) 258, 317, 671, 801
ApeKI GCWGC 1 cut(s) 387
ApoI RAATTY 1 cut(s) 995
Asp700I GAANNNNTTC 1 cut(s) 929
AspS9I GGNCC 1 cut(s) 317
AsuC2I CCSGG 1 cut(s) 1211
AsuHPI GGTGA 1 cut(s) 482
AsuII TTCGAA 1 cut(s) 1159
AvaI CYCGRG 1 cut(s) 759
AxyI CCTNAGG 2 cut(s) 179, 675
BamHI GGATCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 599
BauI CACGAG 1 cut(s) 1029
BbsI GAAGAC 3 cut(s) 617, 942, 1106
Bbv12I GWGCWC 2 cut(s) 550, 599
BbvI GCAGC 1 cut(s) 399
BccI CCATC 5 cut(s) 58, 100, 391, 730, 942
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 1 cut(s) 524
BcnI CCSGG 1 cut(s) 1211
BcoDI GTCTC 3 cut(s) 582, 762, 1211
BfmI CTRYAG 1 cut(s) 954
BfrI CTTAAG 1 cut(s) 1106
BisI GCNGC 2 cut(s) 388, 391
BlpI GCTNAGC 1 cut(s) 659
BlsI GCNGC 2 cut(s) 389, 392
Bme1390I CCNGG 2 cut(s) 524, 1211
BmeT110I CYCGRG 1 cut(s) 759
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 317
BmiI GGNNCC 1 cut(s) 310
BmrFI CCNGG 2 cut(s) 524, 1211
BmrI ACTGGG 2 cut(s) 121, 1180
BmsI GCATC 2 cut(s) 517, 592
BmuI ACTGGG 2 cut(s) 121, 1180
BpiI GAAGAC 3 cut(s) 617, 942, 1106
Bpu1102I GCTNAGC 1 cut(s) 659
Bpu14I TTCGAA 1 cut(s) 1159
BpuEI CTTGAG 1 cut(s) 805
BpuMI CCSGG 1 cut(s) 1211
BsaBI GATNNNNATC 1 cut(s) 513
BsaI GGTCTC 1 cut(s) 1211
BsaJI CCNNGG 3 cut(s) 255, 267, 795
BsaXI ACNNNNNCTCC 8 cut(s) 56, 86, 319, 349, 762, 792, 1191, 1221
Bsc4I CCNNNNNNNGG 8 cut(s) 180, 256, 397, 453, 529, 680, 823, 911
Bse1I ACTGG 2 cut(s) 127, 1175
Bse21I CCTNAGG 2 cut(s) 179, 675
Bse3DI GCAATG 1 cut(s) 1128
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 3 cut(s) 255, 267, 795
BseGI GGATG 6 cut(s) 60, 92, 532, 607, 738, 934
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 8 cut(s) 180, 256, 397, 453, 529, 680, 823, 911
BseMI GCAATG 1 cut(s) 1128
BseMII CTCAG 8 cut(s) 8, 99, 193, 230, 650, 666, 744, 876
BseNI ACTGG 2 cut(s) 127, 1175
BseRI GAGGAG 3 cut(s) 73, 692, 839
BseXI GCAGC 1 cut(s) 399
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 3 cut(s) 315, 1011, 1148
BshFI GGCC 4 cut(s) 260, 319, 673, 803
BsiHKAI GWGCWC 2 cut(s) 550, 599
BsiHKCI CYCGRG 1 cut(s) 759
BsiSI CCGG 1 cut(s) 1210
BslFI GGGAC 2 cut(s) 1187, 1226
BslI CCNNNNNNNGG 8 cut(s) 180, 256, 397, 453, 529, 680, 823, 911
BsmAI GTCTC 3 cut(s) 582, 762, 1211
BsmFI GGGAC 2 cut(s) 1187, 1226
BsmI GAATGC 1 cut(s) 1099
BsnI GGCC 4 cut(s) 260, 319, 673, 803
Bso31I GGTCTC 1 cut(s) 1211
BsoBI CYCGRG 1 cut(s) 759
Bsp119I TTCGAA 1 cut(s) 1159
Bsp1286I GDGCHC 2 cut(s) 550, 599
Bsp143I GATC 2 cut(s) 308, 1059
Bsp1720I GCTNAGC 1 cut(s) 659
BspACI CCGC 6 cut(s) 247, 293, 391, 454, 530, 585
BspANI GGCC 4 cut(s) 260, 319, 673, 803
BspCNI CTCAG 8 cut(s) 9, 98, 192, 229, 651, 667, 745, 877
BspLI GGNNCC 1 cut(s) 310
BspMAI CTGCAG 1 cut(s) 958
BspPI GGATC 2 cut(s) 303, 316
BspT104I TTCGAA 1 cut(s) 1159
BspTI CTTAAG 1 cut(s) 1106
BspTNI GGTCTC 1 cut(s) 1211
BsrBI CCGCTC 1 cut(s) 454
BsrDI GCAATG 1 cut(s) 1128
BsrI ACTGG 2 cut(s) 127, 1175
BssECI CCNNGG 3 cut(s) 255, 267, 795
BssMI GATC 2 cut(s) 308, 1059
BssSI CACGAG 1 cut(s) 1029
BssT1I CCWWGG 3 cut(s) 255, 267, 795
Bst2BI CACGAG 1 cut(s) 1029
Bst2UI CCWGG 1 cut(s) 524
Bst6I CTCTTC 3 cut(s) 74, 142, 735
BstAFI CTTAAG 1 cut(s) 1106
BstBI TTCGAA 1 cut(s) 1159
BstC8I GCNNGC 2 cut(s) 175, 291
BstDEI CTNAG 9 cut(s) 17, 85, 179, 216, 659, 675, 753, 885, 1056
BstF5I GGATG 6 cut(s) 60, 92, 532, 607, 738, 934
BstKTI GATC 2 cut(s) 311, 1062
BstMAI GTCTC 3 cut(s) 582, 762, 1211
BstMBI GATC 2 cut(s) 308, 1059
BstMWI GCNNNNNNNGC 1 cut(s) 436
BstNI CCWGG 1 cut(s) 524
BstNSI RCATGY 1 cut(s) 949
BstSCI CCNGG 2 cut(s) 522, 1209
BstSFI CTRYAG 1 cut(s) 954
BstV1I GCAGC 1 cut(s) 399
BstV2I GAAGAC 3 cut(s) 617, 942, 1106
BstX2I RGATCY 1 cut(s) 308
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 1 cut(s) 308
Bsu36I CCTNAGG 2 cut(s) 179, 675
BsuRI GGCC 4 cut(s) 260, 319, 673, 803
BtgZI GCGATG 1 cut(s) 177
BtrI CACGTC 1 cut(s) 382
BtsCI GGATG 6 cut(s) 60, 92, 532, 607, 738, 934
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 2 cut(s) 175, 291
Cfr13I GGNCC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 1237
CviAII CATG 1 cut(s) 946
CviQI GTAC 1 cut(s) 1237
DdeI CTNAG 9 cut(s) 17, 85, 179, 216, 659, 675, 753, 885, 1056
DpnI GATC 2 cut(s) 310, 1061
DpnII GATC 2 cut(s) 308, 1059
DraI TTTAAA 1 cut(s) 1000
Eam1104I CTCTTC 3 cut(s) 74, 142, 735
EarI CTCTTC 3 cut(s) 74, 142, 735
Ecl136II GAGCTC 1 cut(s) 597
Eco130I CCWWGG 3 cut(s) 255, 267, 795
Eco147I AGGCCT 2 cut(s) 673, 803
Eco24I GRGCYC 1 cut(s) 599
Eco31I GGTCTC 1 cut(s) 1211
Eco32I GATATC 1 cut(s) 813
Eco53kI GAGCTC 1 cut(s) 597
Eco57I CTGAAG 3 cut(s) 142, 446, 1059
Eco81I CCTNAGG 2 cut(s) 179, 675
Eco88I CYCGRG 1 cut(s) 759
EcoICRI GAGCTC 1 cut(s) 597
EcoRII CCWGG 1 cut(s) 522
EcoRV GATATC 1 cut(s) 813
EcoT14I CCWWGG 3 cut(s) 255, 267, 795
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 1 cut(s) 599
ErhI CCWWGG 3 cut(s) 255, 267, 795
FaeI CATG 1 cut(s) 949
FalI AAGNNNNNCTT 2 cut(s) 899, 931
FaqI GGGAC 2 cut(s) 1187, 1226
FatI CATG 1 cut(s) 945
FauI CCCGC 2 cut(s) 254, 523
Fnu4HI GCNGC 2 cut(s) 388, 391
FokI GGATG 6 cut(s) 47, 79, 539, 614, 745, 921
FriOI GRGCYC 1 cut(s) 599
Fsp4HI GCNGC 2 cut(s) 388, 391
GluI GCNGC 2 cut(s) 388, 391
GsaI CCCAGC 1 cut(s) 522
HaeIII GGCC 4 cut(s) 260, 319, 673, 803
HapII CCGG 1 cut(s) 1210
Hin1II CATG 1 cut(s) 949
HindIII AAGCTT 2 cut(s) 287, 428
HinfI GANTC 9 cut(s) 98, 134, 412, 421, 514, 749, 755, 1010, 1175
HpaII CCGG 1 cut(s) 1210
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 2 cut(s) 693, 781
Hpy188III TCNNGA 3 cut(s) 416, 884, 938
Hpy8I GTNNAC 2 cut(s) 693, 781
HpyAV CCTTC 6 cut(s) 246, 261, 460, 568, 679, 905
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 5 cut(s) 332, 605, 956, 1028, 1129
HpyF10VI GCNNNNNNNGC 1 cut(s) 436
HpyF3I CTNAG 9 cut(s) 17, 85, 179, 216, 659, 675, 753, 885, 1056
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 1 cut(s) 949
Kzo9I GATC 2 cut(s) 308, 1059
LmnI GCTCC 2 cut(s) 201, 545
Lsp1109I GCAGC 1 cut(s) 399
LweI GCATC 2 cut(s) 517, 592
MaeII ACGT 1 cut(s) 381
MalI GATC 2 cut(s) 310, 1061
MbiI CCGCTC 1 cut(s) 454
MboI GATC 2 cut(s) 308, 1059
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 2 cut(s) 550, 599
MluCI AATT 3 cut(s) 969, 995, 1255
MlyI GAGTC 7 cut(s) 92, 128, 430, 758, 764, 1019, 1169
MmeI TCCRAC 4 cut(s) 37, 120, 156, 174
Mph1103I ATGCAT 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 929
MseI TTAA 4 cut(s) 999, 1107, 1148, 1258
MspCI CTTAAG 1 cut(s) 1106
MspI CCGG 1 cut(s) 1210
MspR9I CCNGG 2 cut(s) 524, 1211
Mva1269I GAATGC 1 cut(s) 1099
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 1 cut(s) 436
NciI CCSGG 1 cut(s) 1211
NdeII GATC 2 cut(s) 308, 1059
NlaIII CATG 1 cut(s) 949
NlaIV GGNNCC 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 607
NspI RCATGY 1 cut(s) 949
NspV TTCGAA 1 cut(s) 1159
PceI AGGCCT 2 cut(s) 673, 803
PciI ACATGT 1 cut(s) 945
PctI GAATGC 1 cut(s) 1099
PdmI GAANNNNTTC 1 cut(s) 929
PfeI GAWTC 2 cut(s) 412, 514
PfoI TCCNGGA 1 cut(s) 1209
PkrI GCNGC 2 cut(s) 389, 392
PleI GAGTC 7 cut(s) 92, 128, 429, 757, 763, 1018, 1169
PpsI GAGTC 7 cut(s) 92, 128, 429, 757, 763, 1018, 1169
PscI ACATGT 1 cut(s) 945
PsiI TTATAA 2 cut(s) 434, 567
Psp124BI GAGCTC 1 cut(s) 599
Psp6I CCWGG 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 1 cut(s) 310
PspPI GGNCC 1 cut(s) 317
PstI CTGCAG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 308
RsaI GTAC 1 cut(s) 1238
RsaNI GTAC 1 cut(s) 1237
SacI GAGCTC 1 cut(s) 599
SaqAI TTAA 4 cut(s) 999, 1107, 1148, 1258
SatI GCNGC 2 cut(s) 388, 391
Sau3AI GATC 2 cut(s) 308, 1059
Sau96I GGNCC 1 cut(s) 317
SchI GAGTC 7 cut(s) 92, 128, 430, 758, 764, 1019, 1169
ScrFI CCNGG 2 cut(s) 524, 1211
SduI GDGCHC 2 cut(s) 550, 599
SfaNI GCATC 2 cut(s) 517, 592
SfcI CTRYAG 1 cut(s) 954
SfuI TTCGAA 1 cut(s) 1159
SmlI CTYRAG 2 cut(s) 784, 1106
SmoI CTYRAG 2 cut(s) 784, 1106
Sse9I AATT 3 cut(s) 969, 995, 1255
SseBI AGGCCT 2 cut(s) 673, 803
SsiI CCGC 6 cut(s) 247, 293, 391, 454, 530, 585
SstI GAGCTC 1 cut(s) 599
StuI AGGCCT 2 cut(s) 673, 803
StyD4I CCNGG 2 cut(s) 522, 1209
StyI CCWWGG 3 cut(s) 255, 267, 795
TaiI ACGT 1 cut(s) 384
TaqI TCGA 2 cut(s) 845, 1159
TaqII GACCGA 1 cut(s) 629
TasI AATT 3 cut(s) 969, 995, 1255
TatI WGTACW 1 cut(s) 1236
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 2 cut(s) 412, 514
Tru1I TTAA 4 cut(s) 999, 1107, 1148, 1258
Tru9I TTAA 4 cut(s) 999, 1107, 1148, 1258
TscAI CASTG 1 cut(s) 334
TseI GCWGC 1 cut(s) 387
TspDTI ATGAA 3 cut(s) 527, 911, 1008
TspRI CASTG 1 cut(s) 334
Vha464I CTTAAG 1 cut(s) 1106
XapI RAATTY 1 cut(s) 995
XceI RCATGY 1 cut(s) 949
XmnI GAANNNNTTC 1 cut(s) 929
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.