MD03G1077300.v1.1

Acyl-coenzyme A thioesterase 13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000478
Physical Location & Seq
Forward (+)
6277263 .. 6279089
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1077300.v1.1.491

Sequence Viewer

Length: 699 bp
ATGAATATAAGCCCATTCGAACCCGGCCCGCTCAACTTCAGCAAAGATGGATCTGAGTCAAGGTGGTGCAAAAGTCAGCATGGAGAAGGCGTGACCCACGACGAGTCAGATGCGCCGGCGCCGGGCCGAGCTTCTACGAGCCTTACGCTCTTCGCGGCATCCGAGTCGACCGAGTCGAATCCGGACTCGTCGTCTGATTTTTCAAGGTCCCTCTCGCCTCAGTCCAACAACATGGAGAATGCGAAGAAGTCTGTGGAGTTGACGCAGGACGAGTCAGAAGCCTTCTCGCGACTCGTCCTTCCGGAACCCCAACCAGGGGTATGTATATACAATCATTTCGCCACCAGAGGCATCCGAGTCGACCGAGTCGAACCCGGACTCGTTGTCTGTACTCTCAAGGTCCCTCCCCGCCTCACTGATAGAGCTGGAAATTTGGCTAATGGTGCAATTGCAAACCTTGTTGATCAAGTTGGTGCTGTTGTTCATGTTAGGGGTCTCCCGGTGAACGTTTCAGTGGACATTTCCATCTCGTTCATGTCAAAGGCGAAGCTTGGTGATGAGTTAGAGATCACGTCAAGGGTGTTAGGACAACTAGGTCGTTATTGTGGAACATCAATACTTGTGAGAAACAAAGCAACCGGTGAGGTAATTGCTGAAGGTCGGCATTCGTTGTTTGCTATACATACTAGCAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

233

Amino Acids

24.86

Weight (kDa)

5.88

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 143 - 213 6.7e-06 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000478)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29590
fragaria_vesca FvH4_3g37930 FvH4_3g37930 FvH4_3g37970 FvH4_3g37990 FvH4_3g37990 FvH4_3g38000 FvH4_3g38010 FvH4_3g38050 FvH4_3g38060
malus_domestica MD03G1077300.v1.1 MD03G1077400.v1.1 MD03G1077500.v1.1 MD08G1031500.v1.1 MD11G1081500.v1.1 MD11G1081600.v1.1 MD11G1081700.v1.1 MD11G1081800.v1.1 MD11G1081900.v1.1
prunus_persica Prupe.6G061800_v2.0.a1 Prupe.6G061900_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062200_v2.0.a1 Prupe.6G062300_v2.0.a1
pyrus_communis pycom03g06110 pycom03g06120 pycom03g06130 pycom11g06960 pycom11g06970 pycom11g06980 pycom11g07020
rosa_chinensis RchiOBHm_Chr5g0068361 RchiOBHm_Chr5g0068381 RchiOBHm_Chr5g0068391 RchiOBHm_Chr5g0068401 RchiOBHm_Chr5g0068411 RchiOBHm_Chr5g0068441
rosa_laevigata RLG00000035969 RLG00000035971 RLG00000035972 RLG00000035973 RLG00000035974
rosa_multiflora Rmu_sc0001418.1_g000002 Rmu_sc0003450.1_g000002 Rmu_sc0003450.1_g000003 Rmu_sc0003450.1_g000004 Rmu_sc0003450.1_g000007 Rmu_sc0005087.1_g000001 Rmu_sc0006054.1_g000001
rosa_roxburghii Rroxscaffold_1G00012370 Rroxscaffold_1G00012380 Rroxscaffold_1G00012390 Rroxscaffold_1G00012400 Rroxscaffold_1G00012420 Rroxscaffold_1G00013110 Rroxscaffold_1G00013120 Rroxscaffold_1G00013130 Rroxscaffold_1G00013150 Rroxscaffold_1G00013160
rosa_rugosa Rorug05G0389200 Rorug05G0389400 Rorug05G0389500 Rorug05G0389600 Rorug05G0389600
rosa_samantha Rh5AG447900 Rh5AG448100 Rh5AG448200 Rh5AG448300 Rh5AG448400 Rh5BG465700 Rh5BG465900 Rh5BG466200 Rh5BG466300 Rh5BG466700 Rh5CG487500 Rh5CG487800 Rh5CG487900 Rh5CG488300 Rh5DG479500 Rh5DG479700 Rh5DG479800 Rh5DG480100
rosa_wichuraiana Rw5G041800 Rw5G041830 Rw5G041840 Rw5G041850 Rw5G041860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 594
AccB1I GGYRCC 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 2 cut(s) 167, 360
AccII CGCG 2 cut(s) 155, 289
AccIII TCCGGA 2 cut(s) 181, 301
AciI CCGC 3 cut(s) 29, 155, 409
AclI AACGTT 1 cut(s) 507
AclWI GGATC 1 cut(s) 58
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 2 cut(s) 22, 675
AcyI GRCGYC 1 cut(s) 119
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 4 cut(s) 122, 314, 315, 316
AgeI ACCGGT 1 cut(s) 638
AgsI TTSAA 1 cut(s) 204
AhdI GACNNNNNGTC 2 cut(s) 190, 383
AjiI CACGTC 1 cut(s) 573
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 3 cut(s) 131, 425, 550
AluI AGCT 3 cut(s) 131, 425, 550
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 58
Aor13HI TCCGGA 2 cut(s) 181, 301
AoxI GGCC 2 cut(s) 25, 124
ApoI RAATTY 1 cut(s) 430
AsiGI ACCGGT 1 cut(s) 638
AspLEI GCGC 2 cut(s) 115, 121
AspS9I GGNCC 4 cut(s) 26, 124, 207, 400
AsuC2I CCSGG 4 cut(s) 24, 123, 375, 500
AsuHPI GGTGA 3 cut(s) 514, 566, 653
AsuII TTCGAA 1 cut(s) 18
AvaII GGWCC 2 cut(s) 207, 400
BanI GGYRCC 1 cut(s) 118
BccI CCATC 2 cut(s) 41, 533
BcgI CGANNNNNNTGC 6 cut(s) 92, 126, 147, 181, 340, 374
BciT130I CCWGG 1 cut(s) 315
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 4 cut(s) 24, 123, 375, 500
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 2 cut(s) 593, 687
BfoI RGCGCY 1 cut(s) 122
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
Bme1390I CCNGG 5 cut(s) 24, 123, 315, 375, 500
Bme18I GGWCC 2 cut(s) 207, 400
BmeRI GACNNNNNGTC 2 cut(s) 190, 383
BmgBI CACGTC 1 cut(s) 573
BmgT120I GGNCC 4 cut(s) 26, 124, 207, 400
BmiI GGNNCC 4 cut(s) 120, 209, 306, 402
BmrFI CCNGG 5 cut(s) 24, 123, 315, 375, 500
BmsI GCATC 3 cut(s) 100, 167, 360
Bpu14I TTCGAA 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 4 cut(s) 24, 123, 375, 500
BsaHI GRCGYC 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 3 cut(s) 181, 301, 638
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 314, 315, 316
Bse118I RCCGGY 2 cut(s) 115, 638
BseAI TCCGGA 2 cut(s) 181, 301
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 2 cut(s) 158, 351
BseLI CCNNNNNNNGG 4 cut(s) 122, 314, 315, 316
BseMII CTCAG 2 cut(s) 45, 233
Bsh1236I CGCG 2 cut(s) 155, 289
Bsh1285I CGRYCG 2 cut(s) 171, 364
BshFI GGCC 2 cut(s) 27, 126
BshNI GGYRCC 1 cut(s) 118
BshTI ACCGGT 1 cut(s) 638
BsiEI CGRYCG 2 cut(s) 171, 364
BsiSI CCGG 8 cut(s) 24, 116, 122, 182, 302, 375, 500, 639
BslFI GGGAC 2 cut(s) 193, 386
BslI CCNNNNNNNGG 4 cut(s) 122, 314, 315, 316
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 193, 386
BsmI GAATGC 2 cut(s) 244, 664
BsnI GGCC 2 cut(s) 27, 126
Bso31I GGTCTC 1 cut(s) 500
Bsp119I TTCGAA 1 cut(s) 18
Bsp13I TCCGGA 2 cut(s) 181, 301
Bsp143I GATC 3 cut(s) 50, 463, 567
Bsp68I TCGCGA 1 cut(s) 289
BspACI CCGC 3 cut(s) 29, 155, 409
BspANI GGCC 2 cut(s) 27, 126
BspCNI CTCAG 2 cut(s) 46, 232
BspEI TCCGGA 2 cut(s) 181, 301
BspFNI CGCG 2 cut(s) 155, 289
BspLI GGNNCC 4 cut(s) 120, 209, 306, 402
BspPI GGATC 1 cut(s) 58
BspQI GCTCTTC 1 cut(s) 155
BspT104I TTCGAA 1 cut(s) 18
BspT107I GGYRCC 1 cut(s) 118
BspTNI GGTCTC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 31
BsrFI RCCGGY 2 cut(s) 115, 638
BssAI RCCGGY 2 cut(s) 115, 638
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 3 cut(s) 50, 463, 567
BssNI GRCGYC 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 315
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 119
BstBI TTCGAA 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 29, 117
BstDEI CTNAG 2 cut(s) 54, 219
BstF5I GGATG 2 cut(s) 158, 351
BstFNI CGCG 2 cut(s) 155, 289
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 2 cut(s) 115, 121
BstKTI GATC 3 cut(s) 53, 466, 570
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 3 cut(s) 50, 463, 567
BstMCI CGRYCG 2 cut(s) 171, 364
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 5 cut(s) 22, 121, 313, 373, 498
BstUI CGCG 2 cut(s) 155, 289
BstX2I RGATCY 1 cut(s) 50
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 2 cut(s) 27, 126
BtrI CACGTC 1 cut(s) 573
BtsCI GGATG 2 cut(s) 158, 351
BtsIMutI CAGTG 2 cut(s) 414, 519
BtuMI TCGCGA 1 cut(s) 289
Cac8I GCNNGC 2 cut(s) 29, 117
CfoI GCGC 2 cut(s) 115, 121
Cfr10I RCCGGY 2 cut(s) 115, 638
Cfr13I GGNCC 4 cut(s) 26, 124, 207, 400
CseI GACGC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 390
CspAI ACCGGT 1 cut(s) 638
CviAII CATG 4 cut(s) 80, 232, 485, 535
CviJI RGCY 9 cut(s) 12, 27, 126, 131, 141, 281, 425, 437, 550
CviKI_1 RGCY 9 cut(s) 12, 27, 126, 131, 141, 281, 425, 437, 550
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 2 cut(s) 54, 219
DinI GGCGCC 1 cut(s) 120
DpnI GATC 3 cut(s) 52, 465, 569
DpnII GATC 3 cut(s) 50, 463, 567
DrdI GACNNNNNNGTC 1 cut(s) 594
DriI GACNNNNNGTC 2 cut(s) 190, 383
DseDI GACNNNNNNGTC 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 155
Eam1105I GACNNNNNGTC 2 cut(s) 190, 383
EarI CTCTTC 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 2 cut(s) 207, 400
Eco57I CTGAAG 2 cut(s) 22, 675
EcoO109I RGGNCCY 2 cut(s) 207, 400
EcoRII CCWGG 1 cut(s) 313
EgeI GGCGCC 1 cut(s) 120
EheI GGCGCC 1 cut(s) 120
FaeI CATG 4 cut(s) 83, 235, 488, 538
FaqI GGGAC 2 cut(s) 193, 386
FatI CATG 4 cut(s) 79, 231, 484, 534
FauI CCCGC 2 cut(s) 36, 416
FbaI TGATCA 1 cut(s) 463
FblI GTMKAC 2 cut(s) 167, 360
Fnu4HI GCNGC 1 cut(s) 156
FokI GGATG 2 cut(s) 145, 338
Fsp4HI GCNGC 1 cut(s) 156
FspBI CTAG 2 cut(s) 593, 687
GlaI GCGC 2 cut(s) 114, 120
GluI GCNGC 1 cut(s) 156
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 2 cut(s) 27, 126
HapII CCGG 8 cut(s) 24, 116, 122, 182, 302, 375, 500, 639
HgaI GACGC 1 cut(s) 271
HhaI GCGC 2 cut(s) 115, 121
Hin1I GRCGYC 1 cut(s) 119
Hin1II CATG 4 cut(s) 83, 235, 488, 538
Hin6I GCGC 2 cut(s) 113, 119
HinP1I GCGC 2 cut(s) 113, 119
HincII GTYRAC 3 cut(s) 168, 261, 361
HindII GTYRAC 3 cut(s) 168, 261, 361
HindIII AAGCTT 1 cut(s) 548
HpaII CCGG 8 cut(s) 24, 116, 122, 182, 302, 375, 500, 639
HphI GGTGA 3 cut(s) 514, 566, 653
Hpy166II GTNNAC 5 cut(s) 168, 261, 361, 505, 517
Hpy188I TCNGA 7 cut(s) 55, 109, 163, 196, 277, 356, 698
Hpy188III TCNNGA 3 cut(s) 182, 288, 302
Hpy8I GTNNAC 5 cut(s) 168, 261, 361, 505, 517
Hpy99I CGWCG 2 cut(s) 104, 193
HpyAV CCTTC 4 cut(s) 80, 292, 308, 650
HpyCH4IV ACGT 2 cut(s) 507, 572
HpyCH4V TGCA 3 cut(s) 69, 446, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 2 cut(s) 54, 219
HpySE526I ACGT 2 cut(s) 507, 572
Hsp92I GRCGYC 1 cut(s) 119
Hsp92II CATG 4 cut(s) 83, 235, 488, 538
HspAI GCGC 2 cut(s) 113, 119
KasI GGCGCC 1 cut(s) 118
Kpn2I TCCGGA 2 cut(s) 181, 301
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 3 cut(s) 50, 463, 567
LguI GCTCTTC 1 cut(s) 155
LweI GCATC 3 cut(s) 100, 167, 360
MaeI CTAG 2 cut(s) 593, 687
MaeII ACGT 2 cut(s) 507, 572
MaeIII GTNAC 1 cut(s) 91
MalI GATC 3 cut(s) 52, 465, 569
MbiI CCGCTC 1 cut(s) 31
MboI GATC 3 cut(s) 50, 463, 567
MboII GAAGA 2 cut(s) 142, 256
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 1 cut(s) 50
MluCI AATT 3 cut(s) 430, 447, 648
Mly113I GGCGCC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 6 cut(s) 221, 228, 341, 414, 422, 637
MreI CGCCGGCG 1 cut(s) 115
MroI TCCGGA 2 cut(s) 181, 301
MroNI GCCGGC 1 cut(s) 115
MspI CCGG 8 cut(s) 24, 116, 122, 182, 302, 375, 500, 639
MspR9I CCNGG 5 cut(s) 24, 123, 315, 375, 500
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 2 cut(s) 244, 664
MvaI CCWGG 1 cut(s) 315
MvnI CGCG 2 cut(s) 155, 289
MwoI GCNNNNNNNGC 1 cut(s) 443
NaeI GCCGGC 1 cut(s) 117
NarI GGCGCC 1 cut(s) 119
NciI CCSGG 4 cut(s) 24, 123, 375, 500
NdeII GATC 3 cut(s) 50, 463, 567
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 4 cut(s) 83, 235, 488, 538
NlaIV GGNNCC 4 cut(s) 120, 209, 306, 402
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 1 cut(s) 91
NruI TCGCGA 1 cut(s) 289
NspV TTCGAA 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 3 cut(s) 159, 173, 366
PctI GAATGC 2 cut(s) 244, 664
PdiI GCCGGC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 178
PflFI GACNNNGTC 2 cut(s) 172, 365
PinAI ACCGGT 1 cut(s) 638
PkrI GCNGC 1 cut(s) 157
PluTI GGCGCC 1 cut(s) 122
PpuMI RGGWCCY 2 cut(s) 207, 400
Psp1406I AACGTT 1 cut(s) 507
Psp5II RGGWCCY 2 cut(s) 207, 400
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 4 cut(s) 120, 209, 306, 402
PspPI GGNCC 4 cut(s) 26, 124, 207, 400
PspPPI RGGWCCY 2 cut(s) 207, 400
PsuI RGATCY 1 cut(s) 50
PsyI GACNNNGTC 2 cut(s) 172, 365
RruI TCGCGA 1 cut(s) 289
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
SalI GTCGAC 2 cut(s) 166, 359
SapI GCTCTTC 1 cut(s) 155
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 3 cut(s) 50, 463, 567
Sau96I GGNCC 4 cut(s) 26, 124, 207, 400
ScrFI CCNGG 5 cut(s) 24, 123, 315, 375, 500
SfaNI GCATC 3 cut(s) 100, 167, 360
SfoI GGCGCC 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 18
SgrAI CRCCGGYG 1 cut(s) 115
SinI GGWCC 2 cut(s) 207, 400
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 3 cut(s) 430, 447, 648
SsiI CCGC 3 cut(s) 29, 155, 409
SspDI GGCGCC 1 cut(s) 118
SspMI CTAG 2 cut(s) 593, 687
StyD4I CCNGG 5 cut(s) 22, 121, 313, 373, 498
TaiI ACGT 2 cut(s) 510, 575
TaqI TCGA 5 cut(s) 18, 167, 176, 360, 369
TaqII GACCGA 2 cut(s) 185, 378
TasI AATT 3 cut(s) 430, 447, 648
TatI WGTACW 1 cut(s) 389
TauI GCSGC 1 cut(s) 158
TfiI GAWTC 1 cut(s) 178
TscAI CASTG 2 cut(s) 421, 519
TseFI GTSAC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 3 cut(s) 17, 473, 523
TspRI CASTG 2 cut(s) 421, 519
Tth111I GACNNNGTC 2 cut(s) 172, 365
VpaK11BI GGWCC 2 cut(s) 207, 400
XapI RAATTY 1 cut(s) 430
XmiI GTMKAC 2 cut(s) 167, 360
XspI CTAG 2 cut(s) 593, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.