MD03G1077400.v1.1

Acyl-coenzyme A thioesterase 13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000478
Physical Location & Seq
Forward (+)
6281098 .. 6282507
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1077400.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGAGAATGCGAAGAATTCTGTGGAGTTGACGGAGGACGAGTTAGAGGCCTTCTCGCGACTCGTCCTTCCGGAACCCCAAACAGGGGAATGCATATACAATCATTTCGCCATCACAGGCATCCGAGTCGACCGAGTTGAACCCGGATTCGTCGCCTGTACTCTCAAGGTCCCTCCCCGCCTCACTGATAGAGCTGGAAATTTGGCTAATGGTGCAATTGCAAACCTTGTTGATCTAGTTGGTGCTTCTGTTCATTTTAGGGGTCTCCCCATGAACGTTTCGGTGGACATTTCCATCTCGTATCTGTCAAAGGCGAAGCTTGGTGATGAGTTAGAGATCACCTCAAAAAGTTTAGGACAACGAGGTCGTTATTCCGGAGTATCGATACTTGTGAGAAACAAAGCAACTGGGGAGATGATTGCTGAAGGTCGACATTCATTGTTTCGTTCTGCTGCTAGCAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

156

Amino Acids

16.76

Weight (kDa)

6.84

Isoelectric Point (pI)

23.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 66 - 137 5.3e-06 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000478)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29590
fragaria_vesca FvH4_3g37930 FvH4_3g37930 FvH4_3g37970 FvH4_3g37990 FvH4_3g37990 FvH4_3g38000 FvH4_3g38010 FvH4_3g38050 FvH4_3g38060
malus_domestica MD03G1077300.v1.1 MD03G1077400.v1.1 MD03G1077500.v1.1 MD08G1031500.v1.1 MD11G1081500.v1.1 MD11G1081600.v1.1 MD11G1081700.v1.1 MD11G1081800.v1.1 MD11G1081900.v1.1
prunus_persica Prupe.6G061800_v2.0.a1 Prupe.6G061900_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062200_v2.0.a1 Prupe.6G062300_v2.0.a1
pyrus_communis pycom03g06110 pycom03g06120 pycom03g06130 pycom11g06960 pycom11g06970 pycom11g06980 pycom11g07020
rosa_chinensis RchiOBHm_Chr5g0068361 RchiOBHm_Chr5g0068381 RchiOBHm_Chr5g0068391 RchiOBHm_Chr5g0068401 RchiOBHm_Chr5g0068411 RchiOBHm_Chr5g0068441
rosa_laevigata RLG00000035969 RLG00000035971 RLG00000035972 RLG00000035973 RLG00000035974
rosa_multiflora Rmu_sc0001418.1_g000002 Rmu_sc0003450.1_g000002 Rmu_sc0003450.1_g000003 Rmu_sc0003450.1_g000004 Rmu_sc0003450.1_g000007 Rmu_sc0005087.1_g000001 Rmu_sc0006054.1_g000001
rosa_roxburghii Rroxscaffold_1G00012370 Rroxscaffold_1G00012380 Rroxscaffold_1G00012390 Rroxscaffold_1G00012400 Rroxscaffold_1G00012420 Rroxscaffold_1G00013110 Rroxscaffold_1G00013120 Rroxscaffold_1G00013130 Rroxscaffold_1G00013150 Rroxscaffold_1G00013160
rosa_rugosa Rorug05G0389200 Rorug05G0389400 Rorug05G0389500 Rorug05G0389600 Rorug05G0389600
rosa_samantha Rh5AG447900 Rh5AG448100 Rh5AG448200 Rh5AG448300 Rh5AG448400 Rh5BG465700 Rh5BG465900 Rh5BG466200 Rh5BG466300 Rh5BG466700 Rh5CG487500 Rh5CG487800 Rh5CG487900 Rh5CG488300 Rh5DG479500 Rh5DG479700 Rh5DG479800 Rh5DG480100
rosa_wichuraiana Rw5G041800 Rw5G041830 Rw5G041840 Rw5G041850 Rw5G041860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 363
AccI GTMKAC 2 cut(s) 129, 430
AccII CGCG 1 cut(s) 58
AccIII TCCGGA 2 cut(s) 70, 374
AciI CCGC 1 cut(s) 178
AclI AACGTT 1 cut(s) 276
AcsI RAATTY 2 cut(s) 16, 199
AcuI CTGAAG 1 cut(s) 444
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 3 cut(s) 83, 84, 85
AgsI TTSAA 1 cut(s) 140
AluBI AGCT 2 cut(s) 194, 319
AluI AGCT 2 cut(s) 194, 319
Alw26I GTCTC 1 cut(s) 269
Aor13HI TCCGGA 2 cut(s) 70, 374
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 452
ApoI RAATTY 2 cut(s) 16, 199
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 2 cut(s) 331, 335
AsuNHI GCTAGC 1 cut(s) 455
AvaII GGWCC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 439
BccI CCATC 2 cut(s) 119, 302
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 2 cut(s) 236, 456
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 2 cut(s) 75, 171
BmrFI CCNGG 1 cut(s) 144
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 1 cut(s) 129
BmtI GCTAGC 1 cut(s) 459
BmuI ACTGGG 1 cut(s) 417
BplI GAGNNNNNCTC 4 cut(s) 38, 70, 326, 358
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 144
Bsa29I ATCGAT 1 cut(s) 383
BsaI GGTCTC 1 cut(s) 269
BsaWI WCCGGW 2 cut(s) 70, 374
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 84, 85
Bse1I ACTGG 1 cut(s) 412
BseAI TCCGGA 2 cut(s) 70, 374
BseCI ATCGAT 1 cut(s) 383
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 3 cut(s) 83, 84, 85
BseNI ACTGG 1 cut(s) 412
BseXI GCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 58
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 1 cut(s) 50
BshVI ATCGAT 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 133
BsiSI CCGG 3 cut(s) 71, 144, 375
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 3 cut(s) 83, 84, 85
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 2 cut(s) 13, 95
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 269
Bsp13I TCCGGA 2 cut(s) 70, 374
Bsp143I GATC 2 cut(s) 232, 336
Bsp68I TCGCGA 1 cut(s) 58
BspACI CCGC 1 cut(s) 178
BspANI GGCC 1 cut(s) 50
BspDI ATCGAT 1 cut(s) 383
BspEI TCCGGA 2 cut(s) 70, 374
BspFNI CGCG 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 75, 171
BspOI GCTAGC 1 cut(s) 459
BspTNI GGTCTC 1 cut(s) 269
BsrI ACTGG 1 cut(s) 412
BssMI GATC 2 cut(s) 232, 336
BstC8I GCNNGC 1 cut(s) 457
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 58
BstKTI GATC 2 cut(s) 235, 339
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 2 cut(s) 232, 336
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 383
BsuRI GGCC 1 cut(s) 50
BsuTUI ATCGAT 1 cut(s) 383
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 183
BtuMI TCGCGA 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 457
Cfr13I GGNCC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 383
Csp6I GTAC 1 cut(s) 159
CviAII CATG 1 cut(s) 271
CviJI RGCY 4 cut(s) 50, 194, 206, 319
CviKI_1 RGCY 4 cut(s) 50, 194, 206, 319
CviQI GTAC 1 cut(s) 159
DpnI GATC 2 cut(s) 234, 338
DpnII GATC 2 cut(s) 232, 336
DrdI GACNNNNNNGTC 1 cut(s) 363
DseDI GACNNNNNNGTC 1 cut(s) 363
Eco147I AGGCCT 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 169
EcoRI GAATTC 1 cut(s) 16
EcoT22I ATGCAT 1 cut(s) 95
FaeI CATG 1 cut(s) 274
FaiI YATR 3 cut(s) 95, 97, 272
FaqI GGGAC 1 cut(s) 155
FatI CATG 1 cut(s) 270
FauI CCCGC 1 cut(s) 185
FblI GTMKAC 2 cut(s) 129, 430
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 1 cut(s) 107
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 2 cut(s) 236, 456
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 1 cut(s) 50
HapII CCGG 3 cut(s) 71, 144, 375
Hin1II CATG 1 cut(s) 274
HincII GTYRAC 3 cut(s) 30, 130, 431
HindII GTYRAC 3 cut(s) 30, 130, 431
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 3 cut(s) 60, 126, 147
HpaII CCGG 3 cut(s) 71, 144, 375
HphI GGTGA 2 cut(s) 331, 335
Hpy166II GTNNAC 4 cut(s) 30, 130, 286, 431
Hpy188I TCNGA 2 cut(s) 125, 467
Hpy188III TCNNGA 3 cut(s) 57, 71, 375
Hpy8I GTNNAC 4 cut(s) 30, 130, 286, 431
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 61, 77, 419
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 93, 215, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 1 cut(s) 274
Kpn2I TCCGGA 2 cut(s) 70, 374
Kzo9I GATC 2 cut(s) 232, 336
LpnPI CCDG 8 cut(s) 69, 84, 102, 157, 169, 180, 388, 393
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 1 cut(s) 129
MaeI CTAG 2 cut(s) 236, 456
MaeII ACGT 1 cut(s) 276
MalI GATC 2 cut(s) 234, 338
MboI GATC 2 cut(s) 232, 336
MboII GAAGA 1 cut(s) 25
MfeI CAATTG 1 cut(s) 216
MluCI AATT 3 cut(s) 16, 199, 216
MlyI GAGTC 2 cut(s) 54, 135
MnlI CCTC 6 cut(s) 28, 40, 183, 191, 352, 356
Mph1103I ATGCAT 1 cut(s) 95
MroI TCCGGA 2 cut(s) 70, 374
MspI CCGG 3 cut(s) 71, 144, 375
MspR9I CCNGG 1 cut(s) 144
MunI CAATTG 1 cut(s) 216
Mva1269I GAATGC 2 cut(s) 13, 95
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 1 cut(s) 212
NciI CCSGG 1 cut(s) 144
NdeII GATC 2 cut(s) 232, 336
NheI GCTAGC 1 cut(s) 455
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 2 cut(s) 75, 171
NruI TCGCGA 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 95
PceI AGGCCT 1 cut(s) 50
PctI GAATGC 2 cut(s) 13, 95
PfeI GAWTC 1 cut(s) 147
PkrI GCNGC 1 cut(s) 454
PleI GAGTC 2 cut(s) 54, 134
PpsI GAGTC 2 cut(s) 54, 134
PpuMI RGGWCCY 1 cut(s) 169
Psp1406I AACGTT 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 75, 171
PspPI GGNCC 1 cut(s) 169
PspPPI RGGWCCY 1 cut(s) 169
RruI TCGCGA 1 cut(s) 58
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
SalI GTCGAC 2 cut(s) 128, 429
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 2 cut(s) 232, 336
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 2 cut(s) 54, 135
ScrFI CCNGG 1 cut(s) 144
SetI ASST 8 cut(s) 171, 196, 228, 279, 321, 344, 367, 430
SfaNI GCATC 1 cut(s) 129
SinI GGWCC 1 cut(s) 169
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 3 cut(s) 16, 199, 216
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 1 cut(s) 178
SspMI CTAG 2 cut(s) 236, 456
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 1 cut(s) 142
TaiI ACGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 129, 383, 430
TaqII GACCGA 1 cut(s) 147
TasI AATT 3 cut(s) 16, 199, 216
TatI WGTACW 1 cut(s) 158
TfiI GAWTC 1 cut(s) 147
TscAI CASTG 1 cut(s) 190
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 3 cut(s) 242, 287, 426
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 2 cut(s) 16, 199
XmiI GTMKAC 2 cut(s) 129, 430
XspI CTAG 2 cut(s) 236, 456
Zsp2I ATGCAT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.