Rw5G041850

Acyl-coenzyme A thioesterase 13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000478
Physical Location & Seq
Reverse (-)
73508517 .. 73509697
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G041850.1

Sequence Viewer

Length: 516 bp
ATGGATCCTAATGGAGGAGAAGACCAGAAAGCCAGTCTGGAAAAGAAGGCGAATGAGTTTATGCAGCTGAGCCTGGACGAGTCAGAGGCCTTGCTGCGAATCGTCATTCCAGCTCAGAGACCTGGCGAGTCGACCTTCTATGAGAATTTTGCTCTTAGAGGCATCCGAGTTGACCGAGTCGAACCCGGACTCATAGTCTGTACTTTCAAGGTCCCTCCCCGTCTCACTGATAGAGCTGGAAATTTGGCCAATGGGGCAATTGCAAATCTTGTTGATGTAGTTGGATCATATGTGACTTATACTGGTGGTCTCATTAGGAATGTCTCGGTAGATATATCCATCTCGTACATGTCCACGGCAAAGCTTGATGATGAGGTAGAAATCACCTCGAAAAGATTAGGACAAAAAGGAGGTTATACTGGAATAATGGTGCTCCTGAGAAACAAAGCAACTGGGAACATCATTGCTGAAGGTCGACATTCCATGTTTCGTTTACACACTGCTCCGAAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

171

Amino Acids

18.68

Weight (kDa)

7.84

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 80 - 130 6.6e-06 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000478)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29590
fragaria_vesca FvH4_3g37930 FvH4_3g37930 FvH4_3g37970 FvH4_3g37990 FvH4_3g37990 FvH4_3g38000 FvH4_3g38010 FvH4_3g38050 FvH4_3g38060
malus_domestica MD03G1077300.v1.1 MD03G1077400.v1.1 MD03G1077500.v1.1 MD08G1031500.v1.1 MD11G1081500.v1.1 MD11G1081600.v1.1 MD11G1081700.v1.1 MD11G1081800.v1.1 MD11G1081900.v1.1
prunus_persica Prupe.6G061800_v2.0.a1 Prupe.6G061900_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062200_v2.0.a1 Prupe.6G062300_v2.0.a1
pyrus_communis pycom03g06110 pycom03g06120 pycom03g06130 pycom11g06960 pycom11g06970 pycom11g06980 pycom11g07020
rosa_chinensis RchiOBHm_Chr5g0068361 RchiOBHm_Chr5g0068381 RchiOBHm_Chr5g0068391 RchiOBHm_Chr5g0068401 RchiOBHm_Chr5g0068411 RchiOBHm_Chr5g0068441
rosa_laevigata RLG00000035969 RLG00000035971 RLG00000035972 RLG00000035973 RLG00000035974
rosa_multiflora Rmu_sc0001418.1_g000002 Rmu_sc0003450.1_g000002 Rmu_sc0003450.1_g000003 Rmu_sc0003450.1_g000004 Rmu_sc0003450.1_g000007 Rmu_sc0005087.1_g000001 Rmu_sc0006054.1_g000001
rosa_roxburghii Rroxscaffold_1G00012370 Rroxscaffold_1G00012380 Rroxscaffold_1G00012390 Rroxscaffold_1G00012400 Rroxscaffold_1G00012420 Rroxscaffold_1G00013110 Rroxscaffold_1G00013120 Rroxscaffold_1G00013130 Rroxscaffold_1G00013150 Rroxscaffold_1G00013160
rosa_rugosa Rorug05G0389200 Rorug05G0389400 Rorug05G0389500 Rorug05G0389600 Rorug05G0389600
rosa_samantha Rh5AG447900 Rh5AG448100 Rh5AG448200 Rh5AG448300 Rh5AG448400 Rh5BG465700 Rh5BG465900 Rh5BG466200 Rh5BG466300 Rh5BG466700 Rh5CG487500 Rh5CG487800 Rh5CG487900 Rh5CG488300 Rh5DG479500 Rh5DG479700 Rh5DG479800 Rh5DG480100
rosa_wichuraiana Rw5G041800 Rw5G041830 Rw5G041840 Rw5G041850 Rw5G041860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 131, 475
AclWI GGATC 2 cut(s) 12, 292
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 2 cut(s) 145, 241
AcuI CTGAAG 1 cut(s) 489
AfaI GTAC 2 cut(s) 202, 347
AfiI CCNNNNNNNGG 1 cut(s) 14
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 1 cut(s) 208
AhdI GACNNNNNGTC 1 cut(s) 194
AjnI CCWGG 2 cut(s) 72, 121
AluBI AGCT 4 cut(s) 67, 113, 236, 364
AluI AGCT 4 cut(s) 67, 113, 236, 364
Alw21I GWGCWC 1 cut(s) 435
Alw26I GTCTC 4 cut(s) 112, 227, 314, 328
AlwI GGATC 2 cut(s) 12, 292
AoxI GGCC 2 cut(s) 87, 246
ApeKI GCWGC 2 cut(s) 64, 94
ApoI RAATTY 2 cut(s) 145, 241
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 211
BalI TGGCCA 1 cut(s) 248
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 435
BbvI GCAGC 2 cut(s) 76, 81
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 372
BciT130I CCWGG 2 cut(s) 74, 123
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 4 cut(s) 112, 227, 314, 328
BglI GCCNNNNNGGC 1 cut(s) 254
BisI GCNGC 2 cut(s) 65, 95
BlpI GCTNAGC 1 cut(s) 68
BlsI GCNGC 2 cut(s) 66, 96
Bme1390I CCNGG 3 cut(s) 74, 123, 186
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 6, 213
BmrFI CCNGG 3 cut(s) 74, 123, 186
BmrI ACTGGG 1 cut(s) 462
BmsI GCATC 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 27
Bpu1102I GCTNAGC 1 cut(s) 68
BpuMI CCSGG 1 cut(s) 186
BsaI GGTCTC 2 cut(s) 112, 314
BsaJI CCNNGG 1 cut(s) 354
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse1I ACTGG 4 cut(s) 33, 307, 424, 457
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 74, 123
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 14
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 3 cut(s) 59, 128, 428
BseNI ACTGG 4 cut(s) 33, 307, 424, 457
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 2 cut(s) 76, 81
BshFI GGCC 2 cut(s) 89, 248
BsiHKAI GWGCWC 1 cut(s) 435
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 14
BsmAI GTCTC 4 cut(s) 112, 227, 314, 328
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 2 cut(s) 89, 248
Bso31I GGTCTC 2 cut(s) 112, 314
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 2 cut(s) 4, 284
Bsp1720I GCTNAGC 1 cut(s) 68
BspANI GGCC 2 cut(s) 89, 248
BspCNI CTCAG 3 cut(s) 60, 127, 429
BspLI GGNNCC 2 cut(s) 6, 213
BspPI GGATC 2 cut(s) 12, 292
BspTNI GGTCTC 2 cut(s) 112, 314
BsrDI GCAATG 1 cut(s) 462
BsrI ACTGG 4 cut(s) 33, 307, 424, 457
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 2 cut(s) 4, 284
Bst2UI CCWGG 2 cut(s) 74, 123
BstDEI CTNAG 4 cut(s) 68, 114, 155, 437
BstDSI CCRYGG 1 cut(s) 354
BstENI CCTNNNNNAGG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 162
BstKTI GATC 2 cut(s) 7, 287
BstMAI GTCTC 4 cut(s) 112, 227, 314, 328
BstMBI GATC 2 cut(s) 4, 284
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 2 cut(s) 74, 123
BstNSI RCATGY 1 cut(s) 352
BstSCI CCNGG 3 cut(s) 72, 121, 184
BstV1I GCAGC 2 cut(s) 76, 81
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 89, 248
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 225, 498
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 201, 346
CviAII CATG 2 cut(s) 349, 484
CviJI RGCY 8 cut(s) 32, 67, 72, 89, 113, 236, 248, 364
CviKI_1 RGCY 8 cut(s) 32, 67, 72, 89, 113, 236, 248, 364
CviQI GTAC 2 cut(s) 201, 346
DdeI CTNAG 4 cut(s) 68, 114, 155, 437
DpnI GATC 2 cut(s) 6, 286
DpnII GATC 2 cut(s) 4, 284
DriI GACNNNNNGTC 1 cut(s) 194
EaeI YGGCCR 1 cut(s) 246
Eam1105I GACNNNNNGTC 1 cut(s) 194
Eco147I AGGCCT 1 cut(s) 89
Eco31I GGTCTC 2 cut(s) 112, 314
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 489
EcoNI CCTNNNNNAGG 1 cut(s) 12
EcoO109I RGGNCCY 1 cut(s) 211
EcoRII CCWGG 2 cut(s) 72, 121
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 2 cut(s) 352, 487
FaqI GGGAC 1 cut(s) 197
FatI CATG 2 cut(s) 348, 483
FauNDI CATATG 1 cut(s) 289
FblI GTMKAC 2 cut(s) 131, 475
Fnu4HI GCNGC 2 cut(s) 65, 95
FokI GGATG 1 cut(s) 149
Fsp4HI GCNGC 2 cut(s) 65, 95
GluI GCNGC 2 cut(s) 65, 95
HaeIII GGCC 2 cut(s) 89, 248
HapII CCGG 1 cut(s) 186
Hin1II CATG 2 cut(s) 352, 487
HincII GTYRAC 3 cut(s) 132, 172, 476
HindII GTYRAC 3 cut(s) 132, 172, 476
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 5 cut(s) 80, 99, 128, 177, 189
HpaII CCGG 1 cut(s) 186
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 5 cut(s) 132, 172, 354, 476, 494
Hpy188I TCNGA 4 cut(s) 85, 117, 167, 507
Hpy188III TCNNGA 2 cut(s) 38, 436
Hpy8I GTNNAC 5 cut(s) 132, 172, 354, 476, 494
HpyAV CCTTC 3 cut(s) 40, 145, 464
HpyCH4V TGCA 2 cut(s) 64, 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 4 cut(s) 68, 114, 155, 437
Hsp92II CATG 2 cut(s) 352, 487
Kzo9I GATC 2 cut(s) 4, 284
LmnI GCTCC 2 cut(s) 438, 508
Lsp1109I GCAGC 2 cut(s) 76, 81
LweI GCATC 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 6, 286
MboI GATC 2 cut(s) 4, 284
MboII GAAGA 1 cut(s) 32
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 435
MlsI TGGCCA 1 cut(s) 248
MluCI AATT 3 cut(s) 145, 241, 258
MluNI TGGCCA 1 cut(s) 248
MlyI GAGTC 4 cut(s) 89, 137, 183, 186
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 7 cut(s) 8, 79, 152, 225, 367, 397, 404
Mox20I TGGCCA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 248
Msp20I TGGCCA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 3 cut(s) 74, 123, 186
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 2 cut(s) 74, 123
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 4, 284
NlaIII CATG 2 cut(s) 352, 487
NlaIV GGNNCC 2 cut(s) 6, 213
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 1 cut(s) 352
PceI AGGCCT 1 cut(s) 89
PciI ACATGT 1 cut(s) 348
PfeI GAWTC 1 cut(s) 99
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 2 cut(s) 66, 96
PleI GAGTC 4 cut(s) 88, 136, 183, 185
PpsI GAGTC 4 cut(s) 88, 136, 183, 185
PpuMI RGGWCCY 1 cut(s) 211
PscI ACATGT 1 cut(s) 348
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 2 cut(s) 72, 121
PspGI CCWGG 2 cut(s) 72, 121
PspN4I GGNNCC 2 cut(s) 6, 213
PspPI GGNCC 1 cut(s) 211
PspPPI RGGWCCY 1 cut(s) 211
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 176
PvuII CAGCTG 1 cut(s) 67
RsaI GTAC 2 cut(s) 202, 347
RsaNI GTAC 2 cut(s) 201, 346
SalI GTCGAC 2 cut(s) 130, 474
SatI GCNGC 2 cut(s) 65, 95
Sau3AI GATC 2 cut(s) 4, 284
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 4 cut(s) 89, 137, 183, 186
ScrFI CCNGG 3 cut(s) 74, 123, 186
SduI GDGCHC 1 cut(s) 435
SfaNI GCATC 1 cut(s) 171
SinI GGWCC 1 cut(s) 211
Sse9I AATT 3 cut(s) 145, 241, 258
SseBI AGGCCT 1 cut(s) 89
StuI AGGCCT 1 cut(s) 89
StyD4I CCNGG 3 cut(s) 72, 121, 184
TaqI TCGA 4 cut(s) 131, 180, 389, 475
TaqII GACCGA 1 cut(s) 189
TasI AATT 3 cut(s) 145, 241, 258
TatI WGTACW 1 cut(s) 200
TfiI GAWTC 1 cut(s) 99
TscAI CASTG 2 cut(s) 232, 505
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 2 cut(s) 64, 94
Tsp45I GTSAC 1 cut(s) 292
TspRI CASTG 2 cut(s) 232, 505
Tth111I GACNNNGTC 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 211
XagI CCTNNNNNAGG 1 cut(s) 12
XapI RAATTY 2 cut(s) 145, 241
XceI RCATGY 1 cut(s) 352
XmiI GTMKAC 2 cut(s) 131, 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.