Rorug05G0389600

Acyl-coenzyme A thioesterase 13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000478
Physical Location & Seq
Forward (+)
52999990 .. 53006095
6106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0389600.1

Sequence Viewer

Length: 3543 bp
ATGAAGAAGCGGAGGTATTGTGACTTTGCGGGTCGGTCTTCGACTAACGACGGTGAGACGACGGAAGGAAACCGACTTTCGTACTCGGGTCGGGTTTCGGGTTCGGACCAAACCGAAAGAGAGTACTATGAAGACGACGTCGATTCATATAGGCAACCGTTGAAACCGTCGCAGACACATTTGAGCGGCGGCTTGCGTCAATGCGAATCACTGTCGATGAAGTTTCAGTGGAACAATCTGCTGGCCCAGACCCCGAATCCGGGTTCTAACCCGAGAAAGGTTCCGGGTCTGAACCGACCCGACTTGGATTATAGCAACCGGAGTGGTGGTTATAGACTGGATTATGAAATGGGTTATGGCGGAAGGGGTTTGGGTGTGGACCAGAGCAAGCAGTATGGGCATTTTGATCATCGTGGGTTCACGTCGCCATTGCCGAAATCCCATCTTGGTCTCCAAGAAAACGAGGAGGACCATCGCCCAATCCCATATGCGACTCTGGTGGAAGAAGACGAGCCCAAGTTTACTTCTGGAACTGGGTATTCATCAATGGAGGTTTTTCGCGGCGTTCGAGATGAATATGACAGAAGCAGAGCAATGGCTGAGTTGCCAATAGGTCACACGCCTAGACAGAATGAGTTGGTGGACTATGGAGGTTCATCAATTGCTCTGAGTGAGGGTTTTCGCGGCGAGTTCAACAGAAGCAGAGAAATGGGCCATGGTGGATATTCTCAAGGTCAAACAGCGAGACACGATGAGTTGGTAGACAATGCAGGTGGTGCATCAGAAGCTTTGAATGAGGGTTTTCGCGGCAGAAGCAGAGCAATGGCTGAGGGGCCTGTTGGCAGCCATGCTGGATATTCTCAAGGACTCACACCAAGACACTCACATGGGAGTTCACCAATTGCTTTGAATGAGGGTTTTCGCGGTTCGGATGAGTTTAACAAAAGCAGAGCAATGGCTGAGGGGCCTATTGGCCATGGTGGATATTCTCATGGTCAAACACCAAGAAAGAATGAGTTGGTGCACCCCAGGTGGGCTCAGCAGAGTCCGAAAGAGGAAATGGGTAGGGACTACAAGGTTCCTGGAATGAAAAGAAATGACAGTGGTGACATGGATGCCTTGTTCCTTCTTGACTTGCTCTATAAAAAGATGGCTACTGTTGGAGAAGACTCAAGGCCTCCAAGTCATTTGACGCCAAGCGTTGTTGATTCTGTTGTGTACAGGGTTGGTGGTAGCAATGGCTCTGTTGGAGCAGGCACTCATGAGAATTGCCAGTTGGACCAATATCATGATTATATGGAACCAACAAATGTTTTCCCCAACCATCAATCTACAAGTGAACCATCAATCCCCTGCTATGATGAAGTTTTGGGTTCTTCTAATTCAAGAACAGAGGTGTGCCGGAACAATGAAACCCAGGGCTTCAGATTAGGAGTAGATGTTGGTCATGAAAGAGAGGTCGAGCCTTTATCATATGAAGGTAACCTAGAGAGACTGGCTCTGCCTGATGAAGATCAAGGCTTGCTCGAGGCTTATGGTGGTTGCCAACAGAGGTTGGCAGCAGCCGAAAGCCTGGCATCCATGACCCCTCTGGGAAGGTGGGCATTGAGAAAAGTTGCCATGAAGGAGCAACAATCCGGCAATCACTCAAAGTCTAAGTTCTTCCCTGATTTGAACCCCTCTTGTAATGTACAGAACTCGAGCTATGGAGTTGACCTGCAGTTGACTACTACTGAGGATATGCAGCAGCAATATGCTAACAAAGATATTCAACAATATGCTGCTACCTGTGGAGAGGATCATTGGAACATGGAGCAAAATGTGATCTGTGGAGATGGTCAGTGGAACATTGACCAAGGTGTGACCCGTGCAGTGGATCAAAGGAACACTGAACAAGGTTCCATTGACCAAGGTGTGACCTGTGGAGTGGATCAAAGGAACATTGCACAAGGTGTGACCTCTGGAGAGGATCAATGTAACATCCATCGAGGTGTAATCTCCGAAGGGGATCAATGGAACAATGATCAAGGTGTGACAACCTATGGAGAGCAACAATGGCGTATGCACAATGGAGGGGACCAATGGAACAATGATCAAGGCGTCACAACCTCTGGAGAGCAACAGTGGCGTATGAACTATGGAGAGGACCAATGGAATGCTGAGCATATCAACAACCTGAATTCTGACAGTTTGTTACCGAATTCTGAACTGCCAGCATCTACGCAAGAGCATTATGGCGAACAATCCAAATCTGCTAGGATACATGTAAAGGAGCGTTTGGGACCTCCCAAATTATCAACTGGAAGCATAAAAACGCGTTTGAGACCACCTATGAAATCACCTGGCAGTGTGAAGATGCGTTTGGGGCCTTCTGCAAATGATCCTAATATCAACATTTTAGGGTTCAAGCCTCGAGAACAATACAAATCTCGAATAAGAAAAACAGATGACTTCAATGGAAGTATTCATCCTGAAGGGGATGGAATTCCAAAAGCTAAGACTGATGCTGCCGCTGGGAGCATTCATCCTCAGGGGAGTGCTCTTCCACAAGTCAAGACGGATGGTTTTATTGGAAGCATTCATCCTCAAGGGGGTGGTCATTCAATAGTTAAGACAGATGCTACTGGAAGCATTCATCCTCAAGGGAATGGTATTCCAAATGTTAAGAAAGGTGATTTTAATGGAAGCATTCGTCCTCAAGGGGATAGTCTTTCAAAAGTTGATGTGAGAGCTGCAAAGAAAGAACCGCCTGAAGACTCTAAGGAGTTTAAGCAGCTGGTAGATGATGCCTTCATTAAGTTCATTAACATTTTGATTGAGAACCCACCTCAAAGAAGGAAAATCATGGAAGGAAAAGGAGGAAAGTGTTGCGTGTGTGCCAGCAATTCCAAAGAGTTTGTAGAAACGGCAACCTTAGCAAGGCATGCCTTCTTGTCGCCAAAGGTTGGTTTCAAAGCAGAACACCTGGGCTTTCATAAAGCACTTTGTGTGCTCTTGGGATGGGACAGTACAGCAGTCTCAAACACTAGGTGGGTTCAGCGGAATTTGCCAGATGCAGAATCCTTGGCTCTGAAGGAGGACCTAGTTATCTGGCCTCCTGTTGTTGTCATACACAATAGTTCTGTAGCAAATAAAAATCCTGAAGATGAAAGGCTGACGGTAAATGTTGAAGTTCTAAAGGCTATTCTTACAAGTATAGGATTTGTTGGGAGGAAGACCAATATGTACTGTGGGAAACCTGCCGATGGGAGTGTCATGGTGGTGAAGTTTCAGCCTACATTCTCTGGTTTGCAAGAAGCAGAAAGGCTTCACAAGCTGTTTGCTGATAACAAACATGGGAAGGCTGAGTGGCGGTATATCAATTCTGTTGGCCTCAGCAACAGCAGCAATGGAAAGCAAGACTTAGAGGCTGATGAGGCGAATTGTCTATATGGTTATTTGGGAAATGCAGACCATTGGGATGAACTTGACTTGGGAACCAAGAAGCACTTCGGGGTGAAGAGCAAGAAACAAATCAAGGCTATTGCAAATGCTTCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

1180

Amino Acids

130.1

Weight (kDa)

6.03

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 1026 - 1152 1.8e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000478)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29590
fragaria_vesca FvH4_3g37930 FvH4_3g37930 FvH4_3g37970 FvH4_3g37990 FvH4_3g37990 FvH4_3g38000 FvH4_3g38010 FvH4_3g38050 FvH4_3g38060
malus_domestica MD03G1077300.v1.1 MD03G1077400.v1.1 MD03G1077500.v1.1 MD08G1031500.v1.1 MD11G1081500.v1.1 MD11G1081600.v1.1 MD11G1081700.v1.1 MD11G1081800.v1.1 MD11G1081900.v1.1
prunus_persica Prupe.6G061800_v2.0.a1 Prupe.6G061900_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062000_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062100_v2.0.a1 Prupe.6G062200_v2.0.a1 Prupe.6G062300_v2.0.a1
pyrus_communis pycom03g06110 pycom03g06120 pycom03g06130 pycom11g06960 pycom11g06970 pycom11g06980 pycom11g07020
rosa_chinensis RchiOBHm_Chr5g0068361 RchiOBHm_Chr5g0068381 RchiOBHm_Chr5g0068391 RchiOBHm_Chr5g0068401 RchiOBHm_Chr5g0068411 RchiOBHm_Chr5g0068441
rosa_laevigata RLG00000035969 RLG00000035971 RLG00000035972 RLG00000035973 RLG00000035974
rosa_multiflora Rmu_sc0001418.1_g000002 Rmu_sc0003450.1_g000002 Rmu_sc0003450.1_g000003 Rmu_sc0003450.1_g000004 Rmu_sc0003450.1_g000007 Rmu_sc0005087.1_g000001 Rmu_sc0006054.1_g000001
rosa_roxburghii Rroxscaffold_1G00012370 Rroxscaffold_1G00012380 Rroxscaffold_1G00012390 Rroxscaffold_1G00012400 Rroxscaffold_1G00012420 Rroxscaffold_1G00013110 Rroxscaffold_1G00013120 Rroxscaffold_1G00013130 Rroxscaffold_1G00013150 Rroxscaffold_1G00013160
rosa_rugosa Rorug05G0389200 Rorug05G0389400 Rorug05G0389500 Rorug05G0389600 Rorug05G0389600
rosa_samantha Rh5AG447900 Rh5AG448100 Rh5AG448200 Rh5AG448300 Rh5AG448400 Rh5BG465700 Rh5BG465900 Rh5BG466200 Rh5BG466300 Rh5BG466700 Rh5CG487500 Rh5CG487800 Rh5CG487900 Rh5CG488300 Rh5DG479500 Rh5DG479700 Rh5DG479800 Rh5DG480100
rosa_wichuraiana Rw5G041800 Rw5G041830 Rw5G041840 Rw5G041850 Rw5G041860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 761
AatII GACGTC 1 cut(s) 141
Acc36I ACCTGC 3 cut(s) 761, 1725, 3247
AccB7I CCANNNNNTGG 1 cut(s) 2945
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 762
AccII CGCG 5 cut(s) 561, 684, 807, 924, 2317
AclWI GGATC 6 cut(s) 1806, 1884, 1938, 1977, 2016, 2375
AcoI YGGCCR 1 cut(s) 973
AcsI RAATTY 4 cut(s) 2179, 2200, 2484, 3043
AcuI CTGAAG 5 cut(s) 1408, 2493, 2772, 3092, 3162
AcyI GRCGYC 3 cut(s) 138, 1193, 2100
AdeI CACNNNGTG 3 cut(s) 1952, 2987, 3030
AfaI GTAC 6 cut(s) 83, 125, 1220, 1692, 3010, 3227
AfiI CCNNNNNNNGG 9 cut(s) 259, 260, 277, 1033, 1873, 2591, 2919, 2945, 3245
AflIII ACRYGT 2 cut(s) 2263, 2315
AjiI CACGTC 1 cut(s) 423
AjnI CCWGG 6 cut(s) 1028, 1081, 1416, 1572, 2341, 2964
AjuI GAANNNNNNNTTGG 6 cut(s) 1259, 1291, 2345, 2377, 3473, 3505
AloI GAACNNNNNNTCC 4 cut(s) 1106, 1138, 1332, 1364
AluBI AGCT 6 cut(s) 788, 1704, 2495, 2732, 2776, 3316
AluI AGCT 6 cut(s) 788, 1704, 2495, 2732, 2776, 3316
Alw21I GWGCWC 3 cut(s) 1026, 2542, 2994
Alw26I GTCTC 6 cut(s) 50, 455, 739, 1486, 2317, 3022
Alw44I GTGCAC 1 cut(s) 1022
AlwI GGATC 6 cut(s) 1806, 1884, 1938, 1977, 2016, 2375
Ama87I CYCGRG 5 cut(s) 85, 271, 1526, 1699, 2412
AoxI GGCC 9 cut(s) 243, 712, 833, 965, 973, 1175, 2366, 3092, 3370
ApaLI GTGCAC 1 cut(s) 1022
ApoI RAATTY 4 cut(s) 2179, 2200, 2484, 3043
Asp700I GAANNNNTTC 2 cut(s) 3306, 3488
AsuC2I CCSGG 2 cut(s) 261, 285
AsuHPI GGTGA 7 cut(s) 65, 888, 1118, 2331, 2684, 3274, 3508
AvaI CYCGRG 5 cut(s) 85, 271, 1526, 1699, 2412
AvaII GGWCC 8 cut(s) 106, 379, 469, 1279, 2077, 2146, 2282, 3079
AxyI CCTNAGG 1 cut(s) 2529
BaeGI GKGCMC 1 cut(s) 1026
BalI TGGCCA 1 cut(s) 975
BanII GRGCYC 2 cut(s) 516, 1039
BbsI GAAGAC 6 cut(s) 30, 138, 513, 1173, 2760, 3221
Bbv12I GWGCWC 3 cut(s) 1026, 2542, 2994
BbvCI CCTCAGC 3 cut(s) 828, 960, 3374
BceAI ACGGC 1 cut(s) 2922
BciT130I CCWGG 6 cut(s) 1030, 1083, 1418, 1574, 2343, 2966
BciVI GTATCC 1 cut(s) 2253
BclI TGATCA 3 cut(s) 406, 2023, 2092
BcnI CCSGG 2 cut(s) 261, 285
BcoDI GTCTC 6 cut(s) 50, 455, 739, 1486, 2317, 3022
BfaI CTAG 5 cut(s) 624, 1487, 2256, 3027, 3083
BfmI CTRYAG 2 cut(s) 1718, 3123
BfuAI ACCTGC 3 cut(s) 761, 1725, 3247
BfuI GTATCC 1 cut(s) 2253
BlpI GCTNAGC 2 cut(s) 1038, 2160
BmcAI AGTACT 1 cut(s) 125
Bme1390I CCNGG 8 cut(s) 261, 285, 1030, 1083, 1418, 1574, 2343, 2966
Bme18I GGWCC 8 cut(s) 106, 379, 469, 1279, 2077, 2146, 2282, 3079
BmeT110I CYCGRG 5 cut(s) 85, 271, 1526, 1699, 2412
BmgBI CACGTC 1 cut(s) 423
BmrFI CCNGG 8 cut(s) 261, 285, 1030, 1083, 1418, 1574, 2343, 2966
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 9 cut(s) 788, 1105, 1586, 2225, 2346, 2494, 2608, 2776, 3043
BmuI ACTGGG 1 cut(s) 543
BpiI GAAGAC 6 cut(s) 30, 138, 513, 1173, 2760, 3221
BplI GAGNNNNNCTC 2 cut(s) 1483, 1515
BpmI CTGGAG 2 cut(s) 1983, 2133
Bpu10I CCTNAGC 4 cut(s) 828, 960, 2914, 3374
Bpu1102I GCTNAGC 2 cut(s) 1038, 2160
BpuEI CTTGAG 6 cut(s) 714, 846, 1156, 2571, 2625, 2682
BpuMI CCSGG 2 cut(s) 261, 285
BsaBI GATNNNNATC 1 cut(s) 1512
BsaHI GRCGYC 3 cut(s) 138, 1193, 2100
BsaI GGTCTC 2 cut(s) 455, 2317
BsaJI CCNNGG 9 cut(s) 715, 976, 1028, 1416, 1417, 1855, 1909, 2965, 3063
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 5 cut(s) 34, 313, 343, 1425, 1455
Bsc4I CCNNNNNNNGG 9 cut(s) 259, 260, 277, 1033, 1873, 2591, 2919, 2945, 3245
Bse1I ACTGG 6 cut(s) 342, 538, 1273, 1500, 2305, 2629
Bse21I CCTNAGG 1 cut(s) 2529
Bse3DI GCAATG 7 cut(s) 428, 600, 828, 960, 1243, 1941, 3394
Bse8I GATNNNNATC 1 cut(s) 1512
BseBI CCWGG 6 cut(s) 1030, 1083, 1418, 1574, 2343, 2966
BseDI CCNNGG 9 cut(s) 715, 976, 1028, 1416, 1417, 1855, 1909, 2965, 3063
BseJI GATNNNNATC 1 cut(s) 1512
BseLI CCNNNNNNNGG 9 cut(s) 259, 260, 277, 1033, 1873, 2591, 2919, 2945, 3245
BseMI GCAATG 7 cut(s) 428, 600, 828, 960, 1243, 1941, 3394
BseNI ACTGG 6 cut(s) 342, 538, 1273, 1500, 2305, 2629
BseRI GAGGAG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 1026
BseYI CCCAGC 1 cut(s) 2513
BsgI GTGCAG 1 cut(s) 1890
Bsh1236I CGCG 5 cut(s) 561, 684, 807, 924, 2317
BshFI GGCC 9 cut(s) 245, 714, 835, 967, 975, 1177, 2368, 3094, 3372
BsiHKAI GWGCWC 3 cut(s) 1026, 2542, 2994
BsiHKCI CYCGRG 5 cut(s) 85, 271, 1526, 1699, 2412
BsiSI CCGG 5 cut(s) 260, 284, 319, 1402, 1638
BslFI GGGAC 4 cut(s) 1082, 2090, 2295, 3017
BslI CCNNNNNNNGG 9 cut(s) 259, 260, 277, 1033, 1873, 2591, 2919, 2945, 3245
BsmAI GTCTC 6 cut(s) 50, 455, 739, 1486, 2317, 3022
BsmBI CGTCTC 1 cut(s) 50
BsmFI GGGAC 4 cut(s) 1082, 2090, 2295, 3017
BsmI GAATGC 5 cut(s) 2161, 2520, 2577, 2631, 2688
BsnI GGCC 9 cut(s) 245, 714, 835, 967, 975, 1177, 2368, 3094, 3372
Bso31I GGTCTC 2 cut(s) 455, 2317
BsoBI CYCGRG 5 cut(s) 85, 271, 1526, 1699, 2412
Bsp1286I GDGCHC 5 cut(s) 516, 1026, 1039, 2542, 2994
Bsp1407I TGTACA 2 cut(s) 1218, 1690
Bsp1720I GCTNAGC 2 cut(s) 1038, 2160
Bsp19I CCATGG 2 cut(s) 715, 976
BspANI GGCC 9 cut(s) 245, 714, 835, 967, 975, 1177, 2368, 3094, 3372
BspFNI CGCG 5 cut(s) 561, 684, 807, 924, 2317
BspHI TCATGA 3 cut(s) 1261, 1288, 1447
BspMAI CTGCAG 1 cut(s) 1722
BspMI ACCTGC 3 cut(s) 761, 1725, 3247
BspPI GGATC 6 cut(s) 1806, 1884, 1938, 1977, 2016, 2375
BspQI GCTCTTC 2 cut(s) 2547, 3494
BspTNI GGTCTC 2 cut(s) 455, 2317
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 7 cut(s) 428, 600, 828, 960, 1243, 1941, 3394
BsrGI TGTACA 2 cut(s) 1218, 1690
BsrI ACTGG 6 cut(s) 342, 538, 1273, 1500, 2305, 2629
BssECI CCNNGG 9 cut(s) 715, 976, 1028, 1416, 1417, 1855, 1909, 2965, 3063
BssNI GRCGYC 3 cut(s) 138, 1193, 2100
BssT1I CCWWGG 5 cut(s) 715, 976, 1855, 1909, 3063
Bst2UI CCWGG 6 cut(s) 1030, 1083, 1418, 1574, 2343, 2966
Bst6I CTCTTC 2 cut(s) 2547, 3494
BstACI GRCGYC 3 cut(s) 138, 1193, 2100
BstAPI GCANNNNNTGC 2 cut(s) 776, 2924
BstAUI TGTACA 2 cut(s) 1218, 1690
BstC8I GCNNGC 8 cut(s) 194, 243, 389, 1255, 1523, 2214, 2881, 2925
BstDSI CCRYGG 2 cut(s) 715, 976
BstEII GGTNACC 1 cut(s) 1481
BstENI CCTNNNNNAGG 1 cut(s) 2917
BstFNI CGCG 5 cut(s) 561, 684, 807, 924, 2317
BstMAI GTCTC 6 cut(s) 50, 455, 739, 1486, 2317, 3022
BstNI CCWGG 6 cut(s) 1030, 1083, 1418, 1574, 2343, 2966
BstNSI RCATGY 2 cut(s) 2267, 2927
BstPI GGTNACC 1 cut(s) 1481
BstSCI CCNGG 8 cut(s) 259, 283, 1028, 1081, 1416, 1572, 2341, 2964
BstSFI CTRYAG 2 cut(s) 1718, 3123
BstSLI GKGCMC 1 cut(s) 1026
BstUI CGCG 5 cut(s) 561, 684, 807, 924, 2317
BstV2I GAAGAC 6 cut(s) 30, 138, 513, 1173, 2760, 3221
Bsu36I CCTNAGG 1 cut(s) 2529
BsuI GTATCC 1 cut(s) 2253
BsuRI GGCC 9 cut(s) 245, 714, 835, 967, 975, 1177, 2368, 3094, 3372
BtgI CCRYGG 2 cut(s) 715, 976
BtgZI GCGATG 1 cut(s) 458
BtrI CACGTC 1 cut(s) 423
BtsI GCAGTG 2 cut(s) 1878, 2353
BtsIMutI CAGTG 8 cut(s) 209, 233, 1108, 1847, 1878, 1887, 2129, 2353
BveI ACCTGC 3 cut(s) 761, 1725, 3247
Cac8I GCNNGC 8 cut(s) 194, 243, 389, 1255, 1523, 2214, 2881, 2925
CciI TCATGA 3 cut(s) 1261, 1288, 1447
CseI GACGC 3 cut(s) 185, 1201, 2089
Csp6I GTAC 6 cut(s) 82, 124, 1219, 1691, 3009, 3226
CspCI CAANNNNNGTGG 4 cut(s) 304, 339, 754, 789
CviQI GTAC 6 cut(s) 82, 124, 1219, 1691, 3009, 3226
DraIII CACNNNGTG 3 cut(s) 1952, 2987, 3030
EaeI YGGCCR 1 cut(s) 973
Eam1104I CTCTTC 2 cut(s) 2547, 3494
EarI CTCTTC 2 cut(s) 2547, 3494
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 5 cut(s) 715, 976, 1855, 1909, 3063
Eco147I AGGCCT 1 cut(s) 1177
Eco24I GRGCYC 2 cut(s) 516, 1039
Eco31I GGTCTC 2 cut(s) 455, 2317
Eco47I GGWCC 8 cut(s) 106, 379, 469, 1279, 2077, 2146, 2282, 3079
Eco57I CTGAAG 5 cut(s) 1408, 2493, 2772, 3092, 3162
Eco81I CCTNAGG 1 cut(s) 2529
Eco88I CYCGRG 5 cut(s) 85, 271, 1526, 1699, 2412
Eco91I GGTNACC 1 cut(s) 1481
EcoNI CCTNNNNNAGG 1 cut(s) 2917
EcoO109I RGGNCCY 5 cut(s) 833, 965, 2282, 2366, 3079
EcoO65I GGTNACC 1 cut(s) 1481
EcoRI GAATTC 3 cut(s) 2179, 2200, 2484
EcoRII CCWGG 6 cut(s) 1028, 1081, 1416, 1572, 2341, 2964
EcoT14I CCWWGG 5 cut(s) 715, 976, 1855, 1909, 3063
EcoT38I GRGCYC 2 cut(s) 516, 1039
ErhI CCWWGG 5 cut(s) 715, 976, 1855, 1909, 3063
Esp3I CGTCTC 1 cut(s) 50
FalI AAGNNNNNCTT 4 cut(s) 3386, 3418, 3473, 3505
FaqI GGGAC 4 cut(s) 1082, 2090, 2295, 3017
FauI CCCGC 1 cut(s) 22
FauNDI CATATG 2 cut(s) 487, 1474
FbaI TGATCA 3 cut(s) 406, 2023, 2092
FblI GTMKAC 1 cut(s) 762
FriOI GRGCYC 2 cut(s) 516, 1039
FspBI CTAG 5 cut(s) 624, 1487, 2256, 3027, 3083
GsaI CCCAGC 1 cut(s) 2517
GsuI CTGGAG 2 cut(s) 1983, 2133
HaeIII GGCC 9 cut(s) 245, 714, 835, 967, 975, 1177, 2368, 3094, 3372
HapII CCGG 5 cut(s) 260, 284, 319, 1402, 1638
HgaI GACGC 3 cut(s) 185, 1201, 2089
Hin1I GRCGYC 3 cut(s) 138, 1193, 2100
HincII GTYRAC 2 cut(s) 1714, 1725
HindII GTYRAC 2 cut(s) 1714, 1725
HindIII AAGCTT 1 cut(s) 786
HpaII CCGG 5 cut(s) 260, 284, 319, 1402, 1638
HphI GGTGA 7 cut(s) 65, 888, 1118, 2331, 2684, 3274, 3508
Hpy99I CGWCG 6 cut(s) 53, 64, 140, 143, 172, 427
HpyCH4IV ACGT 2 cut(s) 138, 422
HpySE526I ACGT 2 cut(s) 138, 422
Hsp92I GRCGYC 3 cut(s) 138, 1193, 2100
Ksp22I TGATCA 3 cut(s) 406, 2023, 2092
LguI GCTCTTC 2 cut(s) 2547, 3494
LmnI GCTCC 5 cut(s) 1250, 1627, 1813, 2272, 2517
LweI GCATC 9 cut(s) 788, 1105, 1586, 2225, 2346, 2494, 2608, 2776, 3043
MaeI CTAG 5 cut(s) 624, 1487, 2256, 3027, 3083
MaeII ACGT 2 cut(s) 138, 422
MbiI CCGCTC 1 cut(s) 186
MfeI CAATTG 2 cut(s) 660, 900
MhlI GDGCHC 5 cut(s) 516, 1026, 1039, 2542, 2994
MlsI TGGCCA 1 cut(s) 975
MluI ACGCGT 1 cut(s) 2315
MluNI TGGCCA 1 cut(s) 975
MlyI GAGTC 5 cut(s) 487, 861, 1054, 1163, 2750
MmeI TCCRAC 3 cut(s) 1141, 1228, 1257
Mox20I TGGCCA 1 cut(s) 975
MroXI GAANNNNTTC 2 cut(s) 3306, 3488
MscI TGGCCA 1 cut(s) 975
MseI TTAA 7 cut(s) 939, 2610, 2664, 2679, 2769, 2796, 2805
MslI CAYNNNNRTG 4 cut(s) 885, 1989, 3260, 3459
Msp20I TGGCCA 1 cut(s) 975
MspA1I CMGCKG 3 cut(s) 2513, 2776, 3040
MspI CCGG 5 cut(s) 260, 284, 319, 1402, 1638
MspR9I CCNGG 8 cut(s) 261, 285, 1030, 1083, 1418, 1574, 2343, 2966
MunI CAATTG 2 cut(s) 660, 900
Mva1269I GAATGC 5 cut(s) 2161, 2520, 2577, 2631, 2688
MvaI CCWGG 6 cut(s) 1030, 1083, 1418, 1574, 2343, 2966
MvnI CGCG 5 cut(s) 561, 684, 807, 924, 2317
NciI CCSGG 2 cut(s) 261, 285
NcoI CCATGG 2 cut(s) 715, 976
NdeI CATATG 2 cut(s) 487, 1474
NmuCI GTSAC 8 cut(s) 20, 614, 1106, 1861, 1915, 1954, 2032, 2101
NspI RCATGY 2 cut(s) 2267, 2927
PaeI GCATGC 1 cut(s) 2927
PaeR7I CTCGAG 3 cut(s) 1526, 1699, 2412
PagI TCATGA 3 cut(s) 1261, 1288, 1447
PaqCI CACCTGC 1 cut(s) 761
PasI CCCWGGG 1 cut(s) 1417
PceI AGGCCT 1 cut(s) 1177
PciI ACATGT 1 cut(s) 2263
PciSI GCTCTTC 2 cut(s) 2547, 3494
PcsI WCGNNNNNNNCGW 2 cut(s) 431, 565
PctI GAATGC 5 cut(s) 2161, 2520, 2577, 2631, 2688
PdmI GAANNNNTTC 2 cut(s) 3306, 3488
PfeI GAWTC 5 cut(s) 143, 206, 256, 1208, 3059
PflFI GACNNNGTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 2945
PfoI TCCNGGA 1 cut(s) 1081
PleI GAGTC 5 cut(s) 487, 861, 1053, 1163, 2750
PpsI GAGTC 5 cut(s) 487, 861, 1053, 1163, 2750
PpuMI RGGWCCY 2 cut(s) 2282, 3079
PscI ACATGT 1 cut(s) 2263
Psp5II RGGWCCY 2 cut(s) 2282, 3079
Psp6I CCWGG 6 cut(s) 1028, 1081, 1416, 1572, 2341, 2964
PspEI GGTNACC 1 cut(s) 1481
PspFI CCCAGC 1 cut(s) 2513
PspGI CCWGG 6 cut(s) 1028, 1081, 1416, 1572, 2341, 2964
PspPPI RGGWCCY 2 cut(s) 2282, 3079
PspXI VCTCGAGB 2 cut(s) 1526, 1699
PstI CTGCAG 1 cut(s) 1722
PsyI GACNNNGTC 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 2776
RsaI GTAC 6 cut(s) 83, 125, 1220, 1692, 3010, 3227
RsaNI GTAC 6 cut(s) 82, 124, 1219, 1691, 3009, 3226
RseI CAYNNNNRTG 4 cut(s) 885, 1989, 3260, 3459
SapI GCTCTTC 2 cut(s) 2547, 3494
SaqAI TTAA 7 cut(s) 939, 2610, 2664, 2679, 2769, 2796, 2805
ScaI AGTACT 1 cut(s) 125
SchI GAGTC 5 cut(s) 487, 861, 1054, 1163, 2750
ScrFI CCNGG 8 cut(s) 261, 285, 1030, 1083, 1418, 1574, 2343, 2966
SduI GDGCHC 5 cut(s) 516, 1026, 1039, 2542, 2994
SfaNI GCATC 9 cut(s) 788, 1105, 1586, 2225, 2346, 2494, 2608, 2776, 3043
SfcI CTRYAG 2 cut(s) 1718, 3123
Sfr274I CTCGAG 3 cut(s) 1526, 1699, 2412
SinI GGWCC 8 cut(s) 106, 379, 469, 1279, 2077, 2146, 2282, 3079
SlaI CTCGAG 3 cut(s) 1526, 1699, 2412
SmiMI CAYNNNNRTG 4 cut(s) 885, 1989, 3260, 3459
SmlI CTYRAG 9 cut(s) 729, 861, 1171, 1526, 1699, 2412, 2586, 2640, 2697
SmoI CTYRAG 9 cut(s) 729, 861, 1171, 1526, 1699, 2412, 2586, 2640, 2697
SphI GCATGC 1 cut(s) 2927
SseBI AGGCCT 1 cut(s) 1177
SspMI CTAG 5 cut(s) 624, 1487, 2256, 3027, 3083
StuI AGGCCT 1 cut(s) 1177
StyD4I CCNGG 8 cut(s) 259, 283, 1028, 1081, 1416, 1572, 2341, 2964
StyI CCWWGG 5 cut(s) 715, 976, 1855, 1909, 3063
TaiI ACGT 2 cut(s) 141, 425
TaqII GACCGA 1 cut(s) 24
TatI WGTACW 5 cut(s) 123, 1218, 1690, 3008, 3225
TauI GCSGC 6 cut(s) 189, 192, 564, 687, 810, 2513
TfiI GAWTC 5 cut(s) 143, 206, 256, 1208, 3059
Tru1I TTAA 7 cut(s) 939, 2610, 2664, 2679, 2769, 2796, 2805
Tru9I TTAA 7 cut(s) 939, 2610, 2664, 2679, 2769, 2796, 2805
TscAI CASTG 8 cut(s) 216, 233, 1108, 1847, 1878, 1894, 2129, 2353
TseFI GTSAC 8 cut(s) 20, 614, 1106, 1861, 1915, 1954, 2032, 2101
Tsp45I GTSAC 8 cut(s) 20, 614, 1106, 1861, 1915, 1954, 2032, 2101
TspGWI ACGGA 2 cut(s) 77, 2573
TspRI CASTG 8 cut(s) 216, 233, 1108, 1847, 1878, 1894, 2129, 2353
Tth111I GACNNNGTC 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 2945
VneI GTGCAC 1 cut(s) 1022
VpaK11BI GGWCC 8 cut(s) 106, 379, 469, 1279, 2077, 2146, 2282, 3079
XagI CCTNNNNNAGG 1 cut(s) 2917
XapI RAATTY 4 cut(s) 2179, 2200, 2484, 3043
XceI RCATGY 2 cut(s) 2267, 2927
XcmI CCANNNNNNNNNTGG 1 cut(s) 1588
XhoI CTCGAG 3 cut(s) 1526, 1699, 2412
XmiI GTMKAC 1 cut(s) 762
XmnI GAANNNNTTC 2 cut(s) 3306, 3488
XspI CTAG 5 cut(s) 624, 1487, 2256, 3027, 3083
ZraI GACGTC 1 cut(s) 139
ZrmI AGTACT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.