MD03G1280400.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000820
Physical Location & Seq
Forward (+)
36164063 .. 36164561
499 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1280400.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGAAACTTTTCGGCAACTTTGTTATGTTTATTATTTAAAGACTGGGACCTGTAAATTTGGTGCGTCCTGCAAGTTCCACCATCCAAAACATGGAGCTGGATCCTTAAACCGAGCTCCCCTAAATATTTATGGATACCCATTACGACCGGGCGAGAATGAATGCTCCTACTATTTGAAAACGGGGCAATGCAAATTTGGCATAACCTGTAAATTCCATCATCCACAGCCTGCTGGAACAACAATACCGGCTTCTGCACCCCAATTTTATCCGTCGGTGCAGTCTCCTGTTCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

100

Amino Acids

11.08

Weight (kDa)

9.24

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 11 - 30 3.5e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 51 - 76 2.1e-10 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 92
AclWI GGATC 2 cut(s) 96, 109
AcsI RAATTY 3 cut(s) 56, 194, 212
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 1 cut(s) 178
AluBI AGCT 2 cut(s) 98, 116
AluI AGCT 2 cut(s) 98, 116
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 2 cut(s) 96, 109
ApoI RAATTY 3 cut(s) 56, 194, 212
Asp700I GAANNNNTTC 1 cut(s) 9
AspS9I GGNCC 1 cut(s) 48
AsuC2I CCSGG 1 cut(s) 150
AvaII GGWCC 1 cut(s) 48
BamHI GGATCC 1 cut(s) 101
BanII GRGCYC 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 118
BccI CCATC 2 cut(s) 90, 225
BciVI GTATCC 1 cut(s) 128
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 288
BfuI GTATCC 1 cut(s) 128
Bme1390I CCNGG 1 cut(s) 150
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 49, 103
BmrFI CCNGG 1 cut(s) 150
BmrI ACTGGG 1 cut(s) 54
BmuI ACTGGG 1 cut(s) 54
BpuMI CCSGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse118I RCCGGY 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 49
Bse3DI GCAATG 1 cut(s) 194
BseGI GGATG 2 cut(s) 82, 220
BseLI CCNNNNNNNGG 1 cut(s) 92
BseMI GCAATG 1 cut(s) 194
BseNI ACTGG 1 cut(s) 49
BsgI GTGCAG 2 cut(s) 240, 299
Bsh1285I CGRYCG 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 2 cut(s) 149, 248
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 92
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 61
BsmI GAATGC 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 101
BspLI GGNNCC 2 cut(s) 49, 103
BspPI GGATC 2 cut(s) 96, 109
BsrDI GCAATG 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 247
BsrI ACTGG 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 247
BssMI GATC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 231
BstF5I GGATG 2 cut(s) 82, 220
BstKTI GATC 1 cut(s) 104
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 1 cut(s) 101
BstMCI CGRYCG 1 cut(s) 149
BstMWI GCNNNNNNNGC 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuI GTATCC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 82, 220
Cac8I GCNNGC 1 cut(s) 231
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 1 cut(s) 54
CviAII CATG 1 cut(s) 92
CviJI RGCY 4 cut(s) 98, 116, 229, 251
CviKI_1 RGCY 4 cut(s) 98, 116, 229, 251
DpnI GATC 1 cut(s) 103
DpnII GATC 1 cut(s) 101
DraI TTTAAA 1 cut(s) 39
Ecl136II GAGCTC 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 118
FaeI CATG 1 cut(s) 95
FaiI YATR 4 cut(s) 27, 93, 132, 203
FaqI GGGAC 1 cut(s) 61
FatI CATG 1 cut(s) 91
FokI GGATG 2 cut(s) 69, 207
FriOI GRGCYC 1 cut(s) 118
HapII CCGG 2 cut(s) 149, 248
HgaI GACGC 1 cut(s) 54
Hin1II CATG 1 cut(s) 95
HpaII CCGG 2 cut(s) 149, 248
Hpy99I CGWCG 1 cut(s) 277
HpyCH4V TGCA 4 cut(s) 72, 192, 257, 280
HpyF10VI GCNNNNNNNGC 1 cut(s) 198
Hsp92II CATG 1 cut(s) 95
Kzo9I GATC 1 cut(s) 101
LmnI GCTCC 3 cut(s) 95, 121, 170
LpnPI CCDG 9 cut(s) 30, 64, 82, 84, 162, 219, 220, 243, 261
MalI GATC 1 cut(s) 103
MboI GATC 1 cut(s) 101
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 118
MluCI AATT 4 cut(s) 56, 194, 212, 263
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 3 cut(s) 38, 107, 298
MspI CCGG 2 cut(s) 149, 248
MspR9I CCNGG 1 cut(s) 150
Mva1269I GAATGC 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 198
NciI CCSGG 1 cut(s) 150
NdeII GATC 1 cut(s) 101
NlaIII CATG 1 cut(s) 95
NlaIV GGNNCC 2 cut(s) 49, 103
PctI GAATGC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 9
PflMI CCANNNNNTGG 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 118
Psp5II RGGWCCY 1 cut(s) 48
PspN4I GGNNCC 2 cut(s) 49, 103
PspPI GGNCC 1 cut(s) 48
PspPPI RGGWCCY 1 cut(s) 48
PsuI RGATCY 1 cut(s) 101
SacI GAGCTC 1 cut(s) 118
SaqAI TTAA 3 cut(s) 38, 107, 298
Sau3AI GATC 1 cut(s) 101
Sau96I GGNCC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 150
SduI GDGCHC 1 cut(s) 118
SetI ASST 4 cut(s) 53, 100, 118, 209
SinI GGWCC 1 cut(s) 48
Sse9I AATT 4 cut(s) 56, 194, 212, 263
SspI AATATT 1 cut(s) 127
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 1 cut(s) 148
TasI AATT 4 cut(s) 56, 194, 212, 263
Tru1I TTAA 3 cut(s) 38, 107, 298
Tru9I TTAA 3 cut(s) 38, 107, 298
TspDTI ATGAA 1 cut(s) 174
TspGWI ACGGA 1 cut(s) 261
Van91I CCANNNNNTGG 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 48
XapI RAATTY 3 cut(s) 56, 194, 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.