pycom06g14170

3'-UTR-mediated mRNA destabilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000820
Physical Location & Seq
Reverse (-)
19016605 .. 19018608
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g14170.1

Sequence Viewer

Length: 327 bp
ATGGACAAGGCAAAGCAGAGGAGAGAGTTGGCAGAGAAGCAACGCCAGACCAAAAACAAGTATTGCCCAGGACGACGTAAAGGGGAACCCTCCGTAGCTCCAGCTCTGGAACTTAACTTTGTGGGCCTGCCAATTCGTCCGGGGAAGAAAGATTGTCGGCACTATATGCGAACTGTCACTTGCAAGTATGGAAACAGCTGCTTGTTTAATCACCCTGATCCTACACCTGCAAAAGAATCTCGCCCACAATCCGCTTATGAATCTCATGGGATTCATTCCGAAACTACAGAATGGAATGATGTCATCACTCCGTATGGCTGGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
KH_1 zf-CCCH

Protein Analysis

109

Amino Acids

12.43

Weight (kDa)

9.28

Isoelectric Point (pI)

63.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 48 - 72 2.4e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 235
AciI CCGC 1 cut(s) 252
AclWI GGATC 1 cut(s) 212
AjnI CCWGG 1 cut(s) 67
AluBI AGCT 3 cut(s) 98, 104, 198
AluI AGCT 3 cut(s) 98, 104, 198
AlwI GGATC 1 cut(s) 212
AoxI GGCC 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 124
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 1 cut(s) 203
BbvI GCAGC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 69
BcnI CCSGG 1 cut(s) 141
BfmI CTRYAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
Bme1390I CCNGG 2 cut(s) 69, 141
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 69, 141
BpmI CTGGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 67, 140
BseBI CCWGG 1 cut(s) 69
BseDI CCNNGG 2 cut(s) 67, 140
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 1 cut(s) 185
BseYI CCCAGC 1 cut(s) 318
BshFI GGCC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 140
BsnI GGCC 1 cut(s) 126
Bsp143I GATC 1 cut(s) 217
BspACI CCGC 1 cut(s) 252
BspANI GGCC 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 87
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 1 cut(s) 212
BssECI CCNNGG 2 cut(s) 67, 140
BssMI GATC 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 175
BstAPI GCANNNNNTGC 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 128
BstKTI GATC 1 cut(s) 220
BstMBI GATC 1 cut(s) 217
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 69
BstSCI CCNGG 2 cut(s) 67, 139
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 185
BsuRI GGCC 1 cut(s) 126
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 124
CviAII CATG 1 cut(s) 266
CviJI RGCY 5 cut(s) 98, 104, 126, 198, 318
CviKI_1 RGCY 5 cut(s) 98, 104, 126, 198, 318
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 67
FaeI CATG 1 cut(s) 269
FaiI YATR 6 cut(s) 165, 167, 189, 258, 267, 315
FatI CATG 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 199
Fsp4HI GCNGC 1 cut(s) 199
GluI GCNGC 1 cut(s) 199
GsaI CCCAGC 1 cut(s) 322
GsuI CTGGAG 1 cut(s) 84
HaeIII GGCC 1 cut(s) 126
HapII CCGG 1 cut(s) 140
Hin1II CATG 1 cut(s) 269
HinfI GANTC 3 cut(s) 236, 260, 271
HpaII CCGG 1 cut(s) 140
HphI GGTGA 1 cut(s) 203
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 1 cut(s) 107
Hpy99I CGWCG 1 cut(s) 78
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 183, 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 1 cut(s) 269
Kzo9I GATC 1 cut(s) 217
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 1 cut(s) 185
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 1 cut(s) 175
MalI GATC 1 cut(s) 219
MboI GATC 1 cut(s) 217
MboII GAAGA 1 cut(s) 157
MluCI AATT 1 cut(s) 132
MnlI CCTC 2 cut(s) 12, 100
MseI TTAA 2 cut(s) 114, 207
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 2 cut(s) 69, 141
MvaI CCWGG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 1 cut(s) 141
NdeII GATC 1 cut(s) 217
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 235
PfeI GAWTC 3 cut(s) 236, 260, 271
PkrI GCNGC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 318
PspGI CCWGG 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 198
SaqAI TTAA 2 cut(s) 114, 207
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 1 cut(s) 217
Sau96I GGNCC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 69, 141
SetI ASST 5 cut(s) 79, 100, 106, 200, 229
SfcI CTRYAG 1 cut(s) 285
Sse9I AATT 1 cut(s) 132
SsiI CCGC 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 67, 139
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 1 cut(s) 79
TasI AATT 1 cut(s) 132
TfiI GAWTC 3 cut(s) 236, 260, 271
Tru1I TTAA 2 cut(s) 114, 207
Tru9I TTAA 2 cut(s) 114, 207
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 2 cut(s) 263, 273
TspGWI ACGGA 2 cut(s) 82, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.