Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000649

Physical Location & Seq

Genomic Coordinates Reverse (-)

3824076 .. 3827553

Gene Structure 3478 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1034100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1170 bp

ATGGCGACCGAAACAACAGAGCAATCCCCGCCGGCCGACCAAGTCCAGTCGCTTTCAATATCCGAGCCTCAGGCCGATGACCAACCCTCCAGTTCATCAAATCCAACCACTTCATTGAGCTTGGAGGAGAAGTACCAGATCGTGAGGAGCGTGGCGGAGGAGTGTATTCAGGAGGATGAGCTTCGAAATCTGCTGGCTCACAAGACTGAACCCATCTGCTACGACGGGTTCGAACCCTCCGGTCGAATGCACATTGCTCAGGGAGTTATGAAGACTATTAATGTTAACAAGCTGACATCTGCTGGCTGCCGAGTGAAGATATGGATTGCAGATTGGTTTGCGCAGTTGAACAATAAGATGGGGGGTGATTTGAAAAAGATAGAGACAATTGGCCGCTACATGATTGAGATATGGAGGGCTGCGGGGATGAATTTGGACACTGGAAAAGTTGAGTTTTTGTGGTCGTCAAAGGAAATTAATGCGAGAGCACATGAGTACTGGCCTCGTGTGATGGATATAGCTCGGAAAAATAAACTTCCGAGAATAATTAGGTGTAGTCAGATTATGGGTCGAACTGAGCAAGATGAGTTAACGGCAGCCCAAATTCTCTACCCTTGTATGCAGTGTGCAGATATATTCTTTCTTCAGGCAGACATCTGCCAGTTGGGAATGGATCAACGTAAAGTCAATATGCTTGCAAGAGAGTATTGCGATGACATCAAAAAGAAGAACAAGCCTATTATCTTGTCACACCACATGTTACCTGGTTTGCAGCAAGGGCAGGAGAAAATGTCTAAAAGTGATGTGACGTCCTCCATATTAATGGAAGATGAAGAGGCTGAAGTGAATTTGAAGATAAAGAAAGCTTACTGTCCTCCAAATGTTGTCGATGGGAATCCATGTATGGAATATGTGAAGTATCTCATTCTACCTTGGTTCAATGGGTTTTCTGTTGAGCGGACTGAAAAGAATGGTGGAAATAAAACCTTCAAAACCTTTGAAGAATTGGCTGCTGATTATGAAAGCGGCGAGCTACATCCAGCAGACCTTAAATCAGCTTTATCAAAAGCATTGAACAAGATACTGGAGCCTGTACGAGCACACTTCAAGAATGACAAAGCTGCCAAGCAACTATTGCAAAGTGTTAAGAACTATAGAGTCACCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004812 GO:0004831 GO:0005488 GO:0005515 GO:0005524 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006437 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0017076 GO:0019538 GO:0019752 GO:0030554 GO:0031406 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0046483 GO:0046983 GO:0071704 GO:0072545 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_1b

Protein Analysis

390

Amino Acids

44.2

Weight (kDa)

6.43

Isoelectric Point (pI)

51.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1b	PF00579	69 - 363	1.8e-62	tRNA synthetases class I (W and Y)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000649)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33840
fragaria_vesca	FvH4_2g00190 FvH4_4g18133 FvH4_4g36450 FvH4_5g02490 FvH4_5g35150 FvH4_6g35840
malus_domestica	MD04G1034100.v1.1 MD06G1084100.v1.1
prunus_persica	Prupe.2G139100_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1
pyrus_communis	pycom04g02850
rosa_chinensis	RchiOBHm_Chr1g0324941 RchiOBHm_Chr1g0340751 RchiOBHm_Chr2g0133721 RchiOBHm_Chr2g0153921 RchiOBHm_Chr4g0396761 RchiOBHm_Chr4g0414291 RchiOBHm_Chr4g0429871 RchiOBHm_Chr4g0446211 RchiOBHm_Chr5g0057181 RchiOBHm_Chr5g0057391 RchiOBHm_Chr5g0077561 RchiOBHm_Chr6g0307121 RchiOBHm_Chr7g0197611
rosa_laevigata	RLG00000003922 RLG00000005681 RLG00000017313 RLG00000029149 RLG00000035152
rosa_multiflora	Rmu_co8043936.1_g000001 Rmu_sc0001164.1_g000009 Rmu_sc0001334.1_g000004 Rmu_sc0004952.1_g000011 Rmu_sc0013154.1_g000007 Rmu_sc0017650.1_g000006 Rmu_sc0018062.1_g000003
rosa_roxburghii	Rroxscaffold_1G00023030 Rroxscaffold_2G00094650 Rroxscaffold_3G00223000 Rroxscaffold_3G00232150 Rroxscaffold_3G00248030 Rroxscaffold_3G00248040 Rroxscaffold_3G00258320
rosa_rugosa	Rorug04G0184600 Rorug04G0368500 Rorug05G0306000 Rorug05G0307100 Rorug07G0041900
rosa_samantha	Rh2CG164400 Rh2DG389300 Rh3AG306800 Rh4AG430600 Rh4BG440000 Rh4CG457300 Rh4DG438500 Rh5AG375500 Rh5BG386300 Rh5CG409700 Rh5DG400300 Rh6CG481100 Rh7AG166300 Rh7AG360600 Rh7BG168400 Rh7CG174700 Rh7DG168000 Rh7DG387600
rosa_wichuraiana	Rw2G022790 Rw4G036780 Rw5G035320 Rw5G035460 Rw7G014480 Rw7G038030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	812
Acc16I	TGCGCA	1 cut(s)	342
AccBSI	CCGCTC	1 cut(s)	958
AciI	CCGC	6 cut(s)	29, 155, 394, 422, 958, 1026
AclWI	GGATC	1 cut(s)	681
AcoI	YGGCCR	2 cut(s)	33, 391
AcsI	RAATTY	3 cut(s)	430, 603, 847
AcuI	CTGAAG	2 cut(s)	629, 861
AcyI	GRCGYC	1 cut(s)	809
AdeI	CACNNNGTG	1 cut(s)	1167
AfaI	GTAC	3 cut(s)	134, 497, 1095
AflIII	ACRYGT	1 cut(s)	756
AgsI	TTSAA	9 cut(s)	57, 349, 373, 853, 940, 991, 1001, 1075, 1108
AjnI	CCWGG	2 cut(s)	763, 1163
AluBI	AGCT	8 cut(s)	120, 181, 292, 521, 866, 1033, 1058, 1121
AluI	AGCT	8 cut(s)	120, 181, 292, 521, 866, 1033, 1058, 1121
Alw21I	GWGCWC	2 cut(s)	490, 1102
Alw26I	GTCTC	1 cut(s)	377
AlwI	GGATC	1 cut(s)	681
AoxI	GGCC	4 cut(s)	33, 72, 391, 500
ApeKI	GCWGC	6 cut(s)	306, 419, 596, 772, 1010, 1121
ApoI	RAATTY	3 cut(s)	430, 603, 847
AseI	ATTAAT	3 cut(s)	279, 477, 821
AspLEI	GCGC	1 cut(s)	343
AsuHPI	GGTGA	2 cut(s)	377, 1153
AsuII	TTCGAA	2 cut(s)	184, 231
AxyI	CCTNAGG	1 cut(s)	69
BauI	CACGAG	1 cut(s)	504
BbsI	GAAGAC	1 cut(s)	278
Bbv12I	GWGCWC	2 cut(s)	490, 1102
BbvI	GCAGC	6 cut(s)	293, 406, 608, 784, 997, 1108
BccI	CCATC	4 cut(s)	221, 352, 505, 884
BceAI	ACGGC	1 cut(s)	609
BciT130I	CCWGG	2 cut(s)	765, 1165
BcoDI	GTCTC	1 cut(s)	377
BfmI	CTRYAG	1 cut(s)	1153
BisI	GCNGC	8 cut(s)	307, 394, 420, 597, 773, 1011, 1027, 1122
BlsI	GCNGC	8 cut(s)	308, 395, 421, 598, 774, 1012, 1028, 1123
BmcAI	AGTACT	1 cut(s)	497
Bme1390I	CCNGG	2 cut(s)	765, 1165
BmiI	GGNNCC	1 cut(s)	1089
BmrFI	CCNGG	2 cut(s)	765, 1165
BpiI	GAAGAC	1 cut(s)	278
BpmI	CTGGAG	2 cut(s)	73, 1106
Bpu10I	CCTNAGC	1 cut(s)	258
Bpu14I	TTCGAA	2 cut(s)	184, 231
BsaBI	GATNNNNATC	1 cut(s)	894
BsaHI	GRCGYC	1 cut(s)	809
BsaJI	CCNNGG	1 cut(s)	932
BsaWI	WCCGGW	1 cut(s)	239
BsaXI	ACNNNNNCTCC	6 cut(s)	71, 101, 116, 146, 776, 806
Bse118I	RCCGGY	1 cut(s)	31
Bse1I	ACTGG	6 cut(s)	46, 90, 445, 503, 661, 1089
Bse21I	CCTNAGG	1 cut(s)	69
Bse3DI	GCAATG	1 cut(s)	252
Bse8I	GATNNNNATC	1 cut(s)	894
BseBI	CCWGG	2 cut(s)	765, 1165
BseDI	CCNNGG	1 cut(s)	932
BseGI	GGATG	3 cut(s)	181, 432, 1036
BseJI	GATNNNNATC	1 cut(s)	894
BseMI	GCAATG	1 cut(s)	252
BseMII	CTCAG	3 cut(s)	83, 272, 567
BseNI	ACTGG	6 cut(s)	46, 90, 445, 503, 661, 1089
BseRI	GAGGAG	3 cut(s)	140, 160, 173
BseX3I	CGGCCG	1 cut(s)	33
BseXI	GCAGC	6 cut(s)	293, 406, 608, 784, 997, 1108
BsgI	GTGCAG	1 cut(s)	648
Bsh1285I	CGRYCG	3 cut(s)	9, 36, 244
BshFI	GGCC	4 cut(s)	35, 74, 393, 502
BsiEI	CGRYCG	3 cut(s)	9, 36, 244
BsiHKAI	GWGCWC	2 cut(s)	490, 1102
BsiSI	CCGG	2 cut(s)	32, 240
BsmAI	GTCTC	1 cut(s)	377
BsmI	GAATGC	1 cut(s)	252
BsnI	GGCC	4 cut(s)	35, 74, 393, 502
Bsp119I	TTCGAA	2 cut(s)	184, 231
Bsp1286I	GDGCHC	2 cut(s)	490, 1102
Bsp143I	GATC	2 cut(s)	138, 673
BspACI	CCGC	6 cut(s)	29, 155, 394, 422, 958, 1026
BspANI	GGCC	4 cut(s)	35, 74, 393, 502
BspCNI	CTCAG	3 cut(s)	82, 271, 568
BspLI	GGNNCC	1 cut(s)	1089
BspPI	GGATC	1 cut(s)	681
BspT104I	TTCGAA	2 cut(s)	184, 231
BsrBI	CCGCTC	1 cut(s)	958
BsrDI	GCAATG	1 cut(s)	252
BsrFI	RCCGGY	1 cut(s)	31
BsrI	ACTGG	6 cut(s)	46, 90, 445, 503, 661, 1089
BssAI	RCCGGY	1 cut(s)	31
BssECI	CCNNGG	1 cut(s)	932
BssMI	GATC	2 cut(s)	138, 673
BssNI	GRCGYC	1 cut(s)	809
BssSI	CACGAG	1 cut(s)	504
BssT1I	CCWWGG	1 cut(s)	932
Bst2BI	CACGAG	1 cut(s)	504
Bst2UI	CCWGG	2 cut(s)	765, 1165
Bst4CI	ACNGT	1 cut(s)	872
Bst6I	CTCTTC	1 cut(s)	828
BstACI	GRCGYC	1 cut(s)	809
BstAPI	GCANNNNNTGC	1 cut(s)	1135
BstBI	TTCGAA	2 cut(s)	184, 231
BstC8I	GCNNGC	5 cut(s)	33, 195, 304, 696, 1031
BstDEI	CTNAG	3 cut(s)	69, 258, 576
BstF5I	GGATG	3 cut(s)	181, 432, 1036
BstHHI	GCGC	1 cut(s)	343
BstKTI	GATC	2 cut(s)	141, 676
BstMAI	GTCTC	1 cut(s)	377
BstMBI	GATC	2 cut(s)	138, 673
BstMCI	CGRYCG	3 cut(s)	9, 36, 244
BstMWI	GCNNNNNNNGC	3 cut(s)	28, 778, 1135
BstNI	CCWGG	2 cut(s)	765, 1165
BstNSI	RCATGY	1 cut(s)	760
BstSCI	CCNGG	2 cut(s)	763, 1163
BstSFI	CTRYAG	1 cut(s)	1153
BstV1I	GCAGC	6 cut(s)	293, 406, 608, 784, 997, 1108
BstV2I	GAAGAC	1 cut(s)	278
BstXI	CCANNNNNNTGG	1 cut(s)	823
BstZI	CGGCCG	1 cut(s)	33
Bsu36I	CCTNAGG	1 cut(s)	69
BsuRI	GGCC	4 cut(s)	35, 74, 393, 502
BtgZI	GCGATG	1 cut(s)	726
BtsCI	GGATG	3 cut(s)	181, 432, 1036
BtsI	GCAGTG	1 cut(s)	629
BtsIMutI	CAGTG	2 cut(s)	438, 629
Cac8I	GCNNGC	5 cut(s)	33, 195, 304, 696, 1031
CfoI	GCGC	1 cut(s)	343
Cfr10I	RCCGGY	1 cut(s)	31
CsiI	ACCWGGT	2 cut(s)	763, 1163
Csp6I	GTAC	3 cut(s)	133, 496, 1094
CviAII	CATG	4 cut(s)	400, 491, 757, 900
CviQI	GTAC	3 cut(s)	133, 496, 1094
DdeI	CTNAG	3 cut(s)	69, 258, 576
DpnI	GATC	2 cut(s)	140, 675
DpnII	GATC	2 cut(s)	138, 673
DraIII	CACNNNGTG	1 cut(s)	1167
EaeI	YGGCCR	2 cut(s)	33, 391
EagI	CGGCCG	1 cut(s)	33
Eam1104I	CTCTTC	1 cut(s)	828
EarI	CTCTTC	1 cut(s)	828
EciI	GGCGGA	1 cut(s)	170
EclXI	CGGCCG	1 cut(s)	33
Eco130I	CCWWGG	1 cut(s)	932
Eco52I	CGGCCG	1 cut(s)	33
Eco57I	CTGAAG	2 cut(s)	629, 861
Eco81I	CCTNAGG	1 cut(s)	69
EcoRII	CCWGG	2 cut(s)	763, 1163
EcoT14I	CCWWGG	1 cut(s)	932
ErhI	CCWWGG	1 cut(s)	932
FaeI	CATG	4 cut(s)	403, 494, 760, 903
FatI	CATG	4 cut(s)	399, 490, 756, 899
FauI	CCCGC	2 cut(s)	36, 415
Fnu4HI	GCNGC	8 cut(s)	307, 394, 420, 597, 773, 1011, 1027, 1122
FokI	GGATG	3 cut(s)	188, 439, 1023
Fsp4HI	GCNGC	8 cut(s)	307, 394, 420, 597, 773, 1011, 1027, 1122
FspI	TGCGCA	1 cut(s)	342
GlaI	GCGC	1 cut(s)	342
GluI	GCNGC	8 cut(s)	307, 394, 420, 597, 773, 1011, 1027, 1122
GsuI	CTGGAG	2 cut(s)	73, 1106
HaeIII	GGCC	4 cut(s)	35, 74, 393, 502
HapII	CCGG	2 cut(s)	32, 240
HhaI	GCGC	1 cut(s)	343
Hin1I	GRCGYC	1 cut(s)	809
Hin1II	CATG	4 cut(s)	403, 494, 760, 903
Hin6I	GCGC	1 cut(s)	341
HinP1I	GCGC	1 cut(s)	341
HincII	GTYRAC	2 cut(s)	286, 591
HindII	GTYRAC	2 cut(s)	286, 591
HindIII	AAGCTT	1 cut(s)	864
HinfI	GANTC	2 cut(s)	895, 1158
HpaI	GTTAAC	2 cut(s)	286, 591
HpaII	CCGG	2 cut(s)	32, 240
HphI	GGTGA	2 cut(s)	377, 1153
Hpy166II	GTNNAC	2 cut(s)	286, 591
Hpy188I	TCNGA	4 cut(s)	64, 525, 540, 561
Hpy188III	TCNNGA	3 cut(s)	142, 170, 1108
Hpy8I	GTNNAC	2 cut(s)	286, 591
Hpy99I	CGWCG	1 cut(s)	227
HpyAV	CCTTC	1 cut(s)	997
HpyCH4III	ACNGT	1 cut(s)	872
HpyCH4IV	ACGT	2 cut(s)	679, 809
HpyCH4V	TGCA	7 cut(s)	250, 329, 622, 629, 698, 772, 1138
HpyF10VI	GCNNNNNNNGC	3 cut(s)	28, 778, 1135
HpyF3I	CTNAG	3 cut(s)	69, 258, 576
HpySE526I	ACGT	2 cut(s)	679, 809
Hsp92I	GRCGYC	1 cut(s)	809
Hsp92II	CATG	4 cut(s)	403, 494, 760, 903
HspAI	GCGC	1 cut(s)	341
KroI	GCCGGC	1 cut(s)	31
KroNI	GCCGGC	1 cut(s)	33
KspAI	GTTAAC	2 cut(s)	286, 591
Kzo9I	GATC	2 cut(s)	138, 673
LmnI	GCTCC	2 cut(s)	147, 1087
Lsp1109I	GCAGC	6 cut(s)	293, 406, 608, 784, 997, 1108
MabI	ACCWGGT	2 cut(s)	763, 1163
MaeII	ACGT	2 cut(s)	679, 809
MaeIII	GTNAC	4 cut(s)	747, 759, 805, 1159
MalI	GATC	2 cut(s)	140, 675
MbiI	CCGCTC	1 cut(s)	958
MboI	GATC	2 cut(s)	138, 673
MboII	GAAGA	8 cut(s)	283, 328, 635, 739, 839, 845, 865, 1013
MfeI	CAATTG	1 cut(s)	387
MhlI	GDGCHC	2 cut(s)	490, 1102
MluCI	AATT	7 cut(s)	387, 430, 474, 546, 603, 847, 1004
MlyI	GAGTC	1 cut(s)	1167
MmeI	TCCRAC	1 cut(s)	128
MroNI	GCCGGC	1 cut(s)	31
MseI	TTAA	7 cut(s)	279, 285, 477, 590, 821, 1050, 1146
MslI	CAYNNNNRTG	1 cut(s)	821
MspI	CCGG	2 cut(s)	32, 240
MspR9I	CCNGG	2 cut(s)	765, 1165
MunI	CAATTG	1 cut(s)	387
Mva1269I	GAATGC	1 cut(s)	252
MvaI	CCWGG	2 cut(s)	765, 1165
MwoI	GCNNNNNNNGC	3 cut(s)	28, 778, 1135
NaeI	GCCGGC	1 cut(s)	33
NdeII	GATC	2 cut(s)	138, 673
NgoMIV	GCCGGC	1 cut(s)	31
NlaIII	CATG	4 cut(s)	403, 494, 760, 903
NlaIV	GGNNCC	1 cut(s)	1089
NmeAIII	GCCGAG	1 cut(s)	335
NmuCI	GTSAC	3 cut(s)	747, 805, 1159
NsbI	TGCGCA	1 cut(s)	342
NspI	RCATGY	1 cut(s)	760
NspV	TTCGAA	2 cut(s)	184, 231
PciI	ACATGT	1 cut(s)	756
PcsI	WCGNNNNNNNCGW	2 cut(s)	147, 228
PctI	GAATGC	1 cut(s)	252
PdiI	GCCGGC	1 cut(s)	33
PfeI	GAWTC	1 cut(s)	895
PflFI	GACNNNGTC	1 cut(s)	41
PkrI	GCNGC	8 cut(s)	308, 395, 421, 598, 774, 1012, 1028, 1123
PleI	GAGTC	1 cut(s)	1166
PpsI	GAGTC	1 cut(s)	1166
PscI	ACATGT	1 cut(s)	756
PshBI	ATTAAT	3 cut(s)	279, 477, 821
Psp6I	CCWGG	2 cut(s)	763, 1163
PspGI	CCWGG	2 cut(s)	763, 1163
PspN4I	GGNNCC	1 cut(s)	1089
PsyI	GACNNNGTC	1 cut(s)	41
RsaI	GTAC	3 cut(s)	134, 497, 1095
RsaNI	GTAC	3 cut(s)	133, 496, 1094
RseI	CAYNNNNRTG	1 cut(s)	821
SaqAI	TTAA	7 cut(s)	279, 285, 477, 590, 821, 1050, 1146
SatI	GCNGC	8 cut(s)	307, 394, 420, 597, 773, 1011, 1027, 1122
Sau3AI	GATC	2 cut(s)	138, 673
ScaI	AGTACT	1 cut(s)	497
SchI	GAGTC	1 cut(s)	1167
ScrFI	CCNGG	2 cut(s)	765, 1165
SduI	GDGCHC	2 cut(s)	490, 1102
SexAI	ACCWGGT	2 cut(s)	763, 1163
SfcI	CTRYAG	1 cut(s)	1153
SfuI	TTCGAA	2 cut(s)	184, 231
SmiMI	CAYNNNNRTG	1 cut(s)	821
Sse9I	AATT	7 cut(s)	387, 430, 474, 546, 603, 847, 1004
SsiI	CCGC	6 cut(s)	29, 155, 394, 422, 958, 1026
StyD4I	CCNGG	2 cut(s)	763, 1163
StyI	CCWWGG	1 cut(s)	932
TaaI	ACNGT	1 cut(s)	872
TaiI	ACGT	2 cut(s)	682, 812
TaqI	TCGA	5 cut(s)	184, 231, 244, 571, 888
TaqII	GACCGA	1 cut(s)	23
TasI	AATT	7 cut(s)	387, 430, 474, 546, 603, 847, 1004
TatI	WGTACW	1 cut(s)	495
TauI	GCSGC	2 cut(s)	396, 1029
TfiI	GAWTC	1 cut(s)	895
Tru1I	TTAA	7 cut(s)	279, 285, 477, 590, 821, 1050, 1146
Tru9I	TTAA	7 cut(s)	279, 285, 477, 590, 821, 1050, 1146
TscAI	CASTG	2 cut(s)	445, 629
TseFI	GTSAC	3 cut(s)	747, 805, 1159
TseI	GCWGC	6 cut(s)	306, 419, 596, 772, 1010, 1121
Tsp45I	GTSAC	3 cut(s)	747, 805, 1159
TspDTI	ATGAA	6 cut(s)	84, 102, 284, 443, 846, 1035
TspRI	CASTG	2 cut(s)	445, 629
Tth111I	GACNNNGTC	1 cut(s)	41
VspI	ATTAAT	3 cut(s)	279, 477, 821
XapI	RAATTY	3 cut(s)	430, 603, 847
XceI	RCATGY	1 cut(s)	760
XcmI	CCANNNNNNNNNTGG	1 cut(s)	761
ZraI	GACGTC	1 cut(s)	810
ZrmI	AGTACT	1 cut(s)	497

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.