RchiOBHm_Chr4g0446211

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000649
Physical Location & Seq
Reverse (-)
66476661 .. 66478494
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41373

Sequence Viewer

Length: 1071 bp
ATGAATATACATAACACGTACCGGATGGTGAGAAGTGTGGGAGAGGATTGCAGTGAAGAAAAAGAGCTCAAGACTTTGATAGCTAATAAGCCTGAACCTATCTGCTTCAAGCGCTTTCAACCCTCCGGCCTAATGCACATTGCTGAGGGAATTATGGAGATGATGAATGTGAACAAGCTGACTAACGCCGGCTGCAGAGTTAAAATCTTGATTGCAGATCAGTTAGCACGCATGAACAAGAAGTTGGGGGGTGACATGGAGAAGATAGAGACTGTCGGACTATATATGATTGAGGTATGGAAGGCAATGGGGATGAATCTCAAAGATGGCGAAGTAGAGATTTTATGGTCTTCAAAGGAGGTTGGCTCGAGAGTAAATGAGTATTTGGATCTTGTGTCAGATATAACTGACAAAAATAGTCTTGCAAGGTTAAAGAGGTGCTGGAAACTAATGGATCGAACTGATGAAGTAGTCTATTTAAATGCGTCCCAAATTTCTACCTTAGCCATGCAATGTGCAGATATATTTTTCCTACAGGCTGACATCTGCCAGTTTGGAATGGATAAGCGGGAAGTGAACATTCTTGCAAGAGAGTACTGTGATGAAATCCAGAGGAAGAACAAGCCTATTGTCTTGTCACACCATATATTACCCGGTTTGAAGGAAGGGCAGGGGAAAATGTCAAAGAAGATTCCCGATTCTGCCATATTCATGAACGATAATGAGGCTGAAGTAAATCGGAAGATAAAGGAAGCTTTCTGCCCTGAAAAGGTTGTTGAAGGGAATCCATGTTTGGAGTACATTAAGTACCTCATTTTCCCTTGGTTTAATGGATTGGCTGTGGAGCAAATTGAACAGGATGGTGGAGTTATGACCTTCAAAAGCTTTGAAGATTTGATTGTTCATTATCAATGTGGTATTCTACAACCAGATGACCTTAAAGTGGCCTTATCCAAAGCCATTAACAAAATATTGCAGCCCGTAAGAAACCACTTTGAGGATAATGATACAGCTAAACAGCTTTTACAAAGTGTTTTGGTATGTTTCTATTTTGCCTTTCGATTACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_1b

Protein Analysis

356

Amino Acids

40.83

Weight (kDa)

6.05

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1b PF00579 33 - 326 2.2e-43 tRNA synthetases class I (W and Y)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000649)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33840
fragaria_vesca FvH4_2g00190 FvH4_4g18133 FvH4_4g36450 FvH4_5g02490 FvH4_5g35150 FvH4_6g35840
malus_domestica MD04G1034100.v1.1 MD06G1084100.v1.1
prunus_persica Prupe.2G139100_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1
pyrus_communis pycom04g02850
rosa_chinensis RchiOBHm_Chr1g0324941 RchiOBHm_Chr1g0340751 RchiOBHm_Chr2g0133721 RchiOBHm_Chr2g0153921 RchiOBHm_Chr4g0396761 RchiOBHm_Chr4g0414291 RchiOBHm_Chr4g0429871 RchiOBHm_Chr4g0446211 RchiOBHm_Chr5g0057181 RchiOBHm_Chr5g0057391 RchiOBHm_Chr5g0077561 RchiOBHm_Chr6g0307121 RchiOBHm_Chr7g0197611
rosa_laevigata RLG00000003922 RLG00000005681 RLG00000017313 RLG00000029149 RLG00000035152
rosa_multiflora Rmu_co8043936.1_g000001 Rmu_sc0001164.1_g000009 Rmu_sc0001334.1_g000004 Rmu_sc0004952.1_g000011 Rmu_sc0013154.1_g000007 Rmu_sc0017650.1_g000006 Rmu_sc0018062.1_g000003
rosa_roxburghii Rroxscaffold_1G00023030 Rroxscaffold_2G00094650 Rroxscaffold_3G00223000 Rroxscaffold_3G00232150 Rroxscaffold_3G00248030 Rroxscaffold_3G00248040 Rroxscaffold_3G00258320
rosa_rugosa Rorug04G0184600 Rorug04G0368500 Rorug05G0306000 Rorug05G0307100 Rorug07G0041900
rosa_samantha Rh2CG164400 Rh2DG389300 Rh3AG306800 Rh4AG430600 Rh4BG440000 Rh4CG457300 Rh4DG438500 Rh5AG375500 Rh5BG386300 Rh5CG409700 Rh5DG400300 Rh6CG481100 Rh7AG166300 Rh7AG360600 Rh7BG168400 Rh7CG174700 Rh7DG168000 Rh7DG387600
rosa_wichuraiana Rw2G022790 Rw4G036780 Rw5G035320 Rw5G035460 Rw7G014480 Rw7G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 568
AclWI GGATC 2 cut(s) 396, 462
AcsI RAATTY 1 cut(s) 492
AcuI CTGAAG 1 cut(s) 750
AfaI GTAC 4 cut(s) 20, 596, 800, 809
AfeI AGCGCT 1 cut(s) 113
AfiI CCNNNNNNNGG 3 cut(s) 769, 943, 997
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 8 cut(s) 109, 119, 354, 661, 779, 854, 880, 890
AjuI GAANNNNNNNTTGG 4 cut(s) 227, 259, 776, 808
AluBI AGCT 7 cut(s) 67, 83, 178, 755, 885, 1013, 1021
AluI AGCT 7 cut(s) 67, 83, 178, 755, 885, 1013, 1021
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 2 cut(s) 396, 462
Ama87I CYCGRG 1 cut(s) 367
Aor51HI AGCGCT 1 cut(s) 113
AoxI GGCC 2 cut(s) 127, 945
ApeKI GCWGC 2 cut(s) 192, 976
ApoI RAATTY 1 cut(s) 492
AspLEI GCGC 1 cut(s) 114
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 2 cut(s) 40, 263
AvaI CYCGRG 1 cut(s) 367
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 69
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 2 cut(s) 179, 988
BccI CCATC 3 cut(s) 19, 320, 854
BcnI CCSGG 1 cut(s) 654
BcoDI GTCTC 1 cut(s) 263
BfmI CTRYAG 2 cut(s) 193, 533
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 2 cut(s) 193, 977
BlsI GCNGC 2 cut(s) 194, 978
BmcAI AGTACT 1 cut(s) 596
Bme1390I CCNGG 1 cut(s) 654
BmeT110I CYCGRG 1 cut(s) 367
BmrFI CCNGG 1 cut(s) 654
BpiI GAAGAC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 350, 382
Bpu10I CCTNAGC 2 cut(s) 144, 502
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 654
BsaAI YACGTR 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 821
BsaWI WCCGGW 1 cut(s) 21
Bsc4I CCNNNNNNNGG 3 cut(s) 769, 943, 997
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 550
Bse3DI GCAATG 3 cut(s) 138, 312, 518
BseDI CCNNGG 1 cut(s) 821
BseGI GGATG 3 cut(s) 30, 318, 865
BseLI CCNNNNNNNGG 3 cut(s) 769, 943, 997
BseMI GCAATG 3 cut(s) 138, 312, 518
BseMII CTCAG 1 cut(s) 135
BseNI ACTGG 1 cut(s) 550
BseXI GCAGC 2 cut(s) 179, 988
BsgI GTGCAG 1 cut(s) 537
BshFI GGCC 2 cut(s) 129, 947
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 4 cut(s) 22, 126, 189, 654
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 3 cut(s) 769, 943, 997
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 472
BsnI GGCC 2 cut(s) 129, 947
BsoBI CYCGRG 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 3 cut(s) 217, 388, 454
BspACI CCGC 1 cut(s) 568
BspANI GGCC 2 cut(s) 129, 947
BspCNI CTCAG 1 cut(s) 136
BspHI TCATGA 1 cut(s) 711
BspMAI CTGCAG 1 cut(s) 197
BspPI GGATC 2 cut(s) 396, 462
BsrDI GCAATG 3 cut(s) 138, 312, 518
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 1 cut(s) 550
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 1 cut(s) 821
BssMI GATC 3 cut(s) 217, 388, 454
BssT1I CCWWGG 1 cut(s) 821
Bst4CI ACNGT 2 cut(s) 274, 599
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 190, 229
BstDEI CTNAG 2 cut(s) 144, 502
BstF5I GGATG 3 cut(s) 30, 318, 865
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 3 cut(s) 220, 391, 457
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 3 cut(s) 217, 388, 454
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 652
BstSFI CTRYAG 2 cut(s) 193, 533
BstV1I GCAGC 2 cut(s) 179, 988
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 2 cut(s) 129, 947
BtsCI GGATG 3 cut(s) 30, 318, 865
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 58
Cac8I GCNNGC 2 cut(s) 190, 229
CciI TCATGA 1 cut(s) 711
CfoI GCGC 1 cut(s) 114
Cfr10I RCCGGY 1 cut(s) 188
CseI GACGC 1 cut(s) 474
Csp6I GTAC 4 cut(s) 19, 595, 799, 808
CviAII CATG 5 cut(s) 232, 256, 508, 712, 789
CviQI GTAC 4 cut(s) 19, 595, 799, 808
DdeI CTNAG 2 cut(s) 144, 502
DpnI GATC 3 cut(s) 219, 390, 456
DpnII GATC 3 cut(s) 217, 388, 454
DraI TTTAAA 1 cut(s) 480
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 821
Eco24I GRGCYC 1 cut(s) 69
Eco47III AGCGCT 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 750
Eco88I CYCGRG 1 cut(s) 367
EcoICRI GAGCTC 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 821
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 821
FaeI CATG 5 cut(s) 235, 259, 511, 715, 792
FaqI GGGAC 1 cut(s) 472
FatI CATG 5 cut(s) 231, 255, 507, 711, 788
FauI CCCGC 1 cut(s) 561
Fnu4HI GCNGC 2 cut(s) 193, 977
FokI GGATG 3 cut(s) 37, 325, 872
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 2 cut(s) 193, 977
GlaI GCGC 1 cut(s) 113
GluI GCNGC 2 cut(s) 193, 977
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 2 cut(s) 129, 947
HapII CCGG 4 cut(s) 22, 126, 189, 654
HgaI GACGC 1 cut(s) 474
HhaI GCGC 1 cut(s) 114
Hin1II CATG 5 cut(s) 235, 259, 511, 715, 792
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HindIII AAGCTT 2 cut(s) 753, 883
HinfI GANTC 4 cut(s) 316, 691, 698, 784
HpaII CCGG 4 cut(s) 22, 126, 189, 654
HphI GGTGA 2 cut(s) 40, 263
Hpy166II GTNNAC 2 cut(s) 172, 577
Hpy188I TCNGA 3 cut(s) 278, 400, 741
Hpy188III TCNNGA 6 cut(s) 70, 208, 369, 610, 695, 712
Hpy8I GTNNAC 2 cut(s) 172, 577
HpyAV CCTTC 5 cut(s) 295, 655, 659, 773, 886
HpyCH4III ACNGT 2 cut(s) 274, 599
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 9 cut(s) 51, 136, 195, 215, 425, 511, 518, 587, 976
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 2 cut(s) 144, 502
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 5 cut(s) 235, 259, 511, 715, 792
HspAI GCGC 1 cut(s) 112
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Kzo9I GATC 3 cut(s) 217, 388, 454
LmnI GCTCC 1 cut(s) 844
Lsp1109I GCAGC 2 cut(s) 179, 988
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 2 cut(s) 251, 636
MalI GATC 3 cut(s) 219, 390, 456
MboI GATC 3 cut(s) 217, 388, 454
MboII GAAGA 7 cut(s) 68, 274, 342, 628, 700, 754, 902
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 3 cut(s) 150, 492, 849
MmeI TCCRAC 1 cut(s) 256
MroNI GCCGGC 1 cut(s) 188
MseI TTAA 8 cut(s) 201, 431, 479, 804, 828, 939, 963, 1069
MslI CAYNNNNRTG 1 cut(s) 710
MspI CCGG 4 cut(s) 22, 126, 189, 654
MspR9I CCNGG 1 cut(s) 654
MwoI GCNNNNNNNGC 1 cut(s) 111
NaeI GCCGGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 654
NdeII GATC 3 cut(s) 217, 388, 454
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 5 cut(s) 235, 259, 511, 715, 792
NmuCI GTSAC 2 cut(s) 251, 636
PaeR7I CTCGAG 1 cut(s) 367
PagI TCATGA 1 cut(s) 711
PdiI GCCGGC 1 cut(s) 190
PfeI GAWTC 4 cut(s) 316, 691, 698, 784
PkrI GCNGC 2 cut(s) 194, 978
Ppu21I YACGTR 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 69
PstI CTGCAG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 4 cut(s) 20, 596, 800, 809
RsaNI GTAC 4 cut(s) 19, 595, 799, 808
RseI CAYNNNNRTG 1 cut(s) 710
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 8 cut(s) 201, 431, 479, 804, 828, 939, 963, 1069
SatI GCNGC 2 cut(s) 193, 977
Sau3AI GATC 3 cut(s) 217, 388, 454
ScaI AGTACT 1 cut(s) 596
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 69
SfcI CTRYAG 2 cut(s) 193, 533
Sfr274I CTCGAG 1 cut(s) 367
SlaI CTCGAG 1 cut(s) 367
SmiI ATTTAAAT 1 cut(s) 480
SmiMI CAYNNNNRTG 1 cut(s) 710
SmlI CTYRAG 2 cut(s) 68, 367
SmoI CTYRAG 2 cut(s) 68, 367
Sse9I AATT 3 cut(s) 150, 492, 849
SsiI CCGC 1 cut(s) 568
SspI AATATT 1 cut(s) 972
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 652
StyI CCWWGG 1 cut(s) 821
SwaI ATTTAAAT 1 cut(s) 480
TaaI ACNGT 2 cut(s) 274, 599
TaiI ACGT 1 cut(s) 20
TaqI TCGA 3 cut(s) 368, 457, 1060
TasI AATT 3 cut(s) 150, 492, 849
TatI WGTACW 2 cut(s) 594, 798
TfiI GAWTC 4 cut(s) 316, 691, 698, 784
Tru1I TTAA 8 cut(s) 201, 431, 479, 804, 828, 939, 963, 1069
Tru9I TTAA 8 cut(s) 201, 431, 479, 804, 828, 939, 963, 1069
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 2 cut(s) 251, 636
TseI GCWGC 2 cut(s) 192, 976
Tsp45I GTSAC 2 cut(s) 251, 636
TspDTI ATGAA 9 cut(s) 17, 179, 248, 329, 480, 618, 700, 728, 893
TspRI CASTG 1 cut(s) 58
XapI RAATTY 1 cut(s) 492
XhoI CTCGAG 1 cut(s) 367
ZrmI AGTACT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.