Rh4BG440000

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000649
Physical Location & Seq
Reverse (-)
58040123 .. 58043222
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG440000.1

Sequence Viewer

Length: 1362 bp
ATGGCAAACCTAGCAATAGTAGACAACAACAATGTCAATGTCAAGACCTCATCAAAATCAAACTCAAGTACTACTATCCTCAGCCGGTTACATCTACTTGGTTATGGACTTAAAACAGCTTTGCCGAAGCTTAAGCCCCTGAAAATTATTGTGCCGAGAAGAATAAAAGCCTCAGCAATATCATCAAATTCAGCTGCAACCAAGTTCACTGTCACCGGCTGCTTACCTCTCCAATGTTTCAGGTTCGGACTCAAAGCACCAGAGCCGGATCAAACTGTTGACAATGGATCGCTTGCAACCGCTCTCGAGTCTGATTCGGGCCATGTGGTCCAACCCTTCACCTCAACCAAGTCCACAATCCCCACCTGTTTATCTCTCCCTCTCCAAAGTTTGGGACATTTTCAGGAAAGCTTAAAAACAGAGCTATTATCTAAGCCAAGCAAGAGTAATGTAACTAGAAAAGAAGAAGAAGAAGTGTTATCAAAGCGGCCTAATGTGGCAGAAGATTTGATATTAAAAGCAGGCAACAAAGTACTAAAGGAAGGCTCAAAGGGCCAAAGATACTTGTGGAATCTTGCACGACAACACTTCATTAATCGCAAACTCAAGAATACTATCCTAATGAATATACATAACAAGTACCGGATGGTGAGAAGTGTGGGAGAGGATTGCAGTGAAGAAAAAGAGCTCAAGACTTTGATAGCTAATAAGCCTGAACCTATCTGCTTCAAGCGCTTTCAACCCTCCGGCCTAATGCACATTGCTGAGGGAATTATGGAGGTGATGAATGTGAACAAGCTGACTAATGCCGGCTGCAGAGTTAAAATCTTGATTGGGGATCAGTTAGCACGCATGAACAAGAAGTTGGGGGGAGACATGGAGAAGATAGAGACTGTCGGACTATATATGATTGAGGTATGGAAGGCAATGGGGATGAATCTCAAAGATGGCAAAGTAGAGATTTTATGGTCTTCAAAGGAGATTGGCTCGAGAGTAAATGAGTATTTGGATCTTGTGTCAGATATAACTGACAAAAATAGTCTTGCAAGGTTAAAGAGGTGCTGGAAACTAATGGATCCAACTGATGAAGTAGTCTATTTAAATGCGTCCCAAATTTCTACCTTAGGCATGCAATGTGCAGATATATTTTTCCTACAGGCTGACATCTGCCAGTTTGGAATGGATAAGCGGGAAGTGAACATTCTTGCAAGAGAGTACTGTGATGAAATCCAGAGGAAGAACAAGCCTATTGTCTTGTCACACCATACATTACCCGGTTTGAAGGAAGGGCAGGGGAAAATGTCAAAGAAGATTCCCGATTCTGCCATATTCATGAACGATAATGAGGTACATTGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_1b

Protein Analysis

453

Amino Acids

50.71

Weight (kDa)

9.25

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1b PF00579 240 - 450 1.9e-31 tRNA synthetases class I (W and Y)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000649)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33840
fragaria_vesca FvH4_2g00190 FvH4_4g18133 FvH4_4g36450 FvH4_5g02490 FvH4_5g35150 FvH4_6g35840
malus_domestica MD04G1034100.v1.1 MD06G1084100.v1.1
prunus_persica Prupe.2G139100_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1
pyrus_communis pycom04g02850
rosa_chinensis RchiOBHm_Chr1g0324941 RchiOBHm_Chr1g0340751 RchiOBHm_Chr2g0133721 RchiOBHm_Chr2g0153921 RchiOBHm_Chr4g0396761 RchiOBHm_Chr4g0414291 RchiOBHm_Chr4g0429871 RchiOBHm_Chr4g0446211 RchiOBHm_Chr5g0057181 RchiOBHm_Chr5g0057391 RchiOBHm_Chr5g0077561 RchiOBHm_Chr6g0307121 RchiOBHm_Chr7g0197611
rosa_laevigata RLG00000003922 RLG00000005681 RLG00000017313 RLG00000029149 RLG00000035152
rosa_multiflora Rmu_co8043936.1_g000001 Rmu_sc0001164.1_g000009 Rmu_sc0001334.1_g000004 Rmu_sc0004952.1_g000011 Rmu_sc0013154.1_g000007 Rmu_sc0017650.1_g000006 Rmu_sc0018062.1_g000003
rosa_roxburghii Rroxscaffold_1G00023030 Rroxscaffold_2G00094650 Rroxscaffold_3G00223000 Rroxscaffold_3G00232150 Rroxscaffold_3G00248030 Rroxscaffold_3G00248040 Rroxscaffold_3G00258320
rosa_rugosa Rorug04G0184600 Rorug04G0368500 Rorug05G0306000 Rorug05G0307100 Rorug07G0041900
rosa_samantha Rh2CG164400 Rh2DG389300 Rh3AG306800 Rh4AG430600 Rh4BG440000 Rh4CG457300 Rh4DG438500 Rh5AG375500 Rh5BG386300 Rh5CG409700 Rh5DG400300 Rh6CG481100 Rh7AG166300 Rh7AG360600 Rh7BG168400 Rh7CG174700 Rh7DG168000 Rh7DG387600
rosa_wichuraiana Rw2G022790 Rw4G036780 Rw5G035320 Rw5G035460 Rw7G014480 Rw7G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 21
AciI CCGC 3 cut(s) 300, 487, 1189
AclWI GGATC 6 cut(s) 276, 295, 846, 1017, 1070, 1083
AcsI RAATTY 2 cut(s) 187, 1113
AfaI GTAC 5 cut(s) 70, 534, 641, 1217, 1350
AfeI AGCGCT 1 cut(s) 734
AfiI CCNNNNNNNGG 1 cut(s) 391
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 4 cut(s) 730, 740, 975, 1282
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AluBI AGCT 8 cut(s) 119, 130, 194, 411, 424, 688, 704, 799
AluI AGCT 8 cut(s) 119, 130, 194, 411, 424, 688, 704, 799
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 2 cut(s) 867, 884
AlwI GGATC 6 cut(s) 276, 295, 846, 1017, 1070, 1083
Ama87I CYCGRG 2 cut(s) 305, 988
Aor51HI AGCGCT 1 cut(s) 734
AoxI GGCC 4 cut(s) 319, 488, 553, 748
ApeKI GCWGC 3 cut(s) 194, 219, 813
ApoI RAATTY 2 cut(s) 187, 1113
AseI ATTAAT 1 cut(s) 594
AspLEI GCGC 1 cut(s) 735
AspS9I GGNCC 3 cut(s) 319, 328, 553
AsuC2I CCSGG 1 cut(s) 1275
AsuHPI GGTGA 4 cut(s) 205, 331, 661, 793
AvaI CYCGRG 2 cut(s) 305, 988
AvaII GGWCC 1 cut(s) 328
AxyI CCTNAGG 1 cut(s) 1123
BamHI GGATCC 1 cut(s) 1075
BanII GRGCYC 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 963
Bbv12I GWGCWC 1 cut(s) 690
BbvCI CCTCAGC 3 cut(s) 80, 172, 765
BbvI GCAGC 3 cut(s) 181, 206, 800
BccI CCATC 2 cut(s) 640, 941
BcnI CCSGG 1 cut(s) 1275
BcoDI GTCTC 2 cut(s) 867, 884
BfaI CTAG 2 cut(s) 11, 456
BfmI CTRYAG 2 cut(s) 814, 1154
BfoI RGCGCY 1 cut(s) 736
BfrI CTTAAG 1 cut(s) 131
BisI GCNGC 4 cut(s) 195, 220, 488, 814
BlsI GCNGC 4 cut(s) 196, 221, 489, 815
BmcAI AGTACT 3 cut(s) 70, 534, 1217
Bme1390I CCNGG 1 cut(s) 1275
Bme18I GGWCC 1 cut(s) 328
BmeT110I CYCGRG 2 cut(s) 305, 988
BmgT120I GGNCC 3 cut(s) 319, 328, 553
BmiI GGNNCC 1 cut(s) 1077
BmrFI CCNGG 1 cut(s) 1275
BpiI GAAGAC 1 cut(s) 963
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
Bpu10I CCTNAGC 3 cut(s) 80, 172, 765
BpuEI CTTGAG 3 cut(s) 49, 590, 674
BpuMI CCSGG 1 cut(s) 1275
BsaWI WCCGGW 1 cut(s) 642
Bsc4I CCNNNNNNNGG 1 cut(s) 391
Bse118I RCCGGY 3 cut(s) 84, 215, 809
Bse1I ACTGG 1 cut(s) 1171
Bse21I CCTNAGG 1 cut(s) 1123
Bse3DI GCAATG 4 cut(s) 759, 933, 1139, 1351
BseGI GGATG 2 cut(s) 651, 939
BseLI CCNNNNNNNGG 1 cut(s) 391
BseMI GCAATG 4 cut(s) 759, 933, 1139, 1351
BseMII CTCAG 3 cut(s) 94, 186, 756
BseNI ACTGG 1 cut(s) 1171
BseXI GCAGC 3 cut(s) 181, 206, 800
BsgI GTGCAG 1 cut(s) 1158
BshFI GGCC 4 cut(s) 321, 490, 555, 750
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 2 cut(s) 305, 988
BsiSI CCGG 7 cut(s) 85, 216, 266, 643, 747, 810, 1275
BslFI GGGAC 2 cut(s) 408, 1093
BslI CCNNNNNNNGG 1 cut(s) 391
BsmAI GTCTC 2 cut(s) 867, 884
BsmFI GGGAC 2 cut(s) 408, 1093
BsnI GGCC 4 cut(s) 321, 490, 555, 750
BsoBI CYCGRG 2 cut(s) 305, 988
Bsp1286I GDGCHC 1 cut(s) 690
Bsp143I GATC 5 cut(s) 268, 287, 838, 1009, 1075
BspACI CCGC 3 cut(s) 300, 487, 1189
BspANI GGCC 4 cut(s) 321, 490, 555, 750
BspCNI CTCAG 3 cut(s) 93, 185, 757
BspHI TCATGA 1 cut(s) 1332
BspLI GGNNCC 1 cut(s) 1077
BspMAI CTGCAG 1 cut(s) 818
BspPI GGATC 6 cut(s) 276, 295, 846, 1017, 1070, 1083
BspTI CTTAAG 1 cut(s) 131
BsrBI CCGCTC 1 cut(s) 302
BsrDI GCAATG 4 cut(s) 759, 933, 1139, 1351
BsrFI RCCGGY 3 cut(s) 84, 215, 809
BsrI ACTGG 1 cut(s) 1171
BssAI RCCGGY 3 cut(s) 84, 215, 809
BssMI GATC 5 cut(s) 268, 287, 838, 1009, 1075
Bst4CI ACNGT 4 cut(s) 211, 277, 895, 1220
BstAFI CTTAAG 1 cut(s) 131
BstC8I GCNNGC 5 cut(s) 294, 523, 811, 850, 1130
BstDEI CTNAG 5 cut(s) 80, 172, 432, 765, 1123
BstF5I GGATG 2 cut(s) 651, 939
BstH2I RGCGCY 1 cut(s) 736
BstHHI GCGC 1 cut(s) 735
BstKTI GATC 5 cut(s) 271, 290, 841, 1012, 1078
BstMAI GTCTC 2 cut(s) 867, 884
BstMBI GATC 5 cut(s) 268, 287, 838, 1009, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 11, 552, 732
BstNSI RCATGY 1 cut(s) 1132
BstSCI CCNGG 1 cut(s) 1273
BstSFI CTRYAG 2 cut(s) 814, 1154
BstV1I GCAGC 3 cut(s) 181, 206, 800
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 2 cut(s) 1009, 1075
BstYI RGATCY 2 cut(s) 1009, 1075
Bsu36I CCTNAGG 1 cut(s) 1123
BsuRI GGCC 4 cut(s) 321, 490, 555, 750
BtsCI GGATG 2 cut(s) 651, 939
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 2 cut(s) 207, 679
Cac8I GCNNGC 5 cut(s) 294, 523, 811, 850, 1130
CciI TCATGA 1 cut(s) 1332
CfoI GCGC 1 cut(s) 735
Cfr10I RCCGGY 3 cut(s) 84, 215, 809
Cfr13I GGNCC 3 cut(s) 319, 328, 553
CseI GACGC 1 cut(s) 1095
Csp6I GTAC 5 cut(s) 69, 533, 640, 1216, 1349
CviAII CATG 5 cut(s) 323, 853, 877, 1129, 1333
CviQI GTAC 5 cut(s) 69, 533, 640, 1216, 1349
DdeI CTNAG 5 cut(s) 80, 172, 432, 765, 1123
DpnI GATC 5 cut(s) 270, 289, 840, 1011, 1077
DpnII GATC 5 cut(s) 268, 287, 838, 1009, 1075
DraI TTTAAA 1 cut(s) 1101
Ecl136II GAGCTC 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 690
Eco47I GGWCC 1 cut(s) 328
Eco47III AGCGCT 1 cut(s) 734
Eco53kI GAGCTC 1 cut(s) 688
Eco81I CCTNAGG 1 cut(s) 1123
Eco88I CYCGRG 2 cut(s) 305, 988
EcoICRI GAGCTC 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 690
FaeI CATG 5 cut(s) 326, 856, 880, 1132, 1336
FaqI GGGAC 2 cut(s) 408, 1093
FatI CATG 5 cut(s) 322, 852, 876, 1128, 1332
FauI CCCGC 1 cut(s) 1182
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 4 cut(s) 195, 220, 488, 814
FokI GGATG 2 cut(s) 658, 946
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 4 cut(s) 195, 220, 488, 814
FspBI CTAG 2 cut(s) 11, 456
GlaI GCGC 1 cut(s) 734
GluI GCNGC 4 cut(s) 195, 220, 488, 814
HaeII RGCGCY 1 cut(s) 736
HaeIII GGCC 4 cut(s) 321, 490, 555, 750
HapII CCGG 7 cut(s) 85, 216, 266, 643, 747, 810, 1275
HgaI GACGC 1 cut(s) 1095
HhaI GCGC 1 cut(s) 735
Hin1II CATG 5 cut(s) 326, 856, 880, 1132, 1336
Hin6I GCGC 1 cut(s) 733
HinP1I GCGC 1 cut(s) 733
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 2 cut(s) 128, 409
HinfI GANTC 7 cut(s) 249, 308, 314, 571, 937, 1312, 1319
HpaII CCGG 7 cut(s) 85, 216, 266, 643, 747, 810, 1275
HphI GGTGA 4 cut(s) 205, 331, 661, 793
Hpy166II GTNNAC 6 cut(s) 22, 207, 280, 354, 793, 1198
Hpy188I TCNGA 4 cut(s) 248, 313, 899, 1021
Hpy8I GTNNAC 6 cut(s) 22, 207, 280, 354, 793, 1198
HpyAV CCTTC 5 cut(s) 346, 536, 916, 1276, 1280
HpyCH4III ACNGT 4 cut(s) 211, 277, 895, 1220
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 552, 732
HpyF3I CTNAG 5 cut(s) 80, 172, 432, 765, 1123
Hsp92II CATG 5 cut(s) 326, 856, 880, 1132, 1336
HspAI GCGC 1 cut(s) 733
KroI GCCGGC 1 cut(s) 809
KroNI GCCGGC 1 cut(s) 811
Kzo9I GATC 5 cut(s) 268, 287, 838, 1009, 1075
Lsp1109I GCAGC 3 cut(s) 181, 206, 800
MaeI CTAG 2 cut(s) 11, 456
MaeIII GTNAC 4 cut(s) 87, 211, 451, 1257
MalI GATC 5 cut(s) 270, 289, 840, 1011, 1077
MbiI CCGCTC 1 cut(s) 302
MboI GATC 5 cut(s) 268, 287, 838, 1009, 1075
MflI RGATCY 2 cut(s) 1009, 1075
MhlI GDGCHC 1 cut(s) 690
MluCI AATT 4 cut(s) 144, 187, 771, 1113
MlyI GAGTC 2 cut(s) 243, 317
MmeI TCCRAC 3 cut(s) 355, 877, 1103
MroNI GCCGGC 1 cut(s) 809
MseI TTAA 8 cut(s) 111, 132, 413, 515, 594, 822, 1052, 1100
MslI CAYNNNNRTG 1 cut(s) 1331
MspA1I CMGCKG 1 cut(s) 194
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 7 cut(s) 85, 216, 266, 643, 747, 810, 1275
MspR9I CCNGG 1 cut(s) 1275
MwoI GCNNNNNNNGC 3 cut(s) 11, 552, 732
NaeI GCCGGC 1 cut(s) 811
NciI CCSGG 1 cut(s) 1275
NdeII GATC 5 cut(s) 268, 287, 838, 1009, 1075
NgoMIV GCCGGC 1 cut(s) 809
NlaIII CATG 5 cut(s) 326, 856, 880, 1132, 1336
NlaIV GGNNCC 1 cut(s) 1077
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 211, 1257
NspI RCATGY 1 cut(s) 1132
PaeI GCATGC 1 cut(s) 1132
PaeR7I CTCGAG 2 cut(s) 305, 988
PagI TCATGA 1 cut(s) 1332
PdiI GCCGGC 1 cut(s) 811
PfeI GAWTC 5 cut(s) 314, 571, 937, 1312, 1319
PflMI CCANNNNNTGG 1 cut(s) 391
PkrI GCNGC 4 cut(s) 196, 221, 489, 815
PleI GAGTC 2 cut(s) 243, 316
PpsI GAGTC 2 cut(s) 243, 316
PshBI ATTAAT 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 690
PspN4I GGNNCC 1 cut(s) 1077
PspPI GGNCC 3 cut(s) 319, 328, 553
PstI CTGCAG 1 cut(s) 818
PsuI RGATCY 2 cut(s) 1009, 1075
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 5 cut(s) 70, 534, 641, 1217, 1350
RsaNI GTAC 5 cut(s) 69, 533, 640, 1216, 1349
RseI CAYNNNNRTG 1 cut(s) 1331
SacI GAGCTC 1 cut(s) 690
SaqAI TTAA 8 cut(s) 111, 132, 413, 515, 594, 822, 1052, 1100
SatI GCNGC 4 cut(s) 195, 220, 488, 814
Sau3AI GATC 5 cut(s) 268, 287, 838, 1009, 1075
Sau96I GGNCC 3 cut(s) 319, 328, 553
ScaI AGTACT 3 cut(s) 70, 534, 1217
SchI GAGTC 2 cut(s) 243, 317
ScrFI CCNGG 1 cut(s) 1275
SduI GDGCHC 1 cut(s) 690
SfcI CTRYAG 2 cut(s) 814, 1154
Sfr274I CTCGAG 2 cut(s) 305, 988
SinI GGWCC 1 cut(s) 328
SlaI CTCGAG 2 cut(s) 305, 988
SmiI ATTTAAAT 1 cut(s) 1101
SmiMI CAYNNNNRTG 1 cut(s) 1331
SmlI CTYRAG 6 cut(s) 64, 131, 305, 605, 689, 988
SmoI CTYRAG 6 cut(s) 64, 131, 305, 605, 689, 988
SphI GCATGC 1 cut(s) 1132
Sse9I AATT 4 cut(s) 144, 187, 771, 1113
SsiI CCGC 3 cut(s) 300, 487, 1189
SspMI CTAG 2 cut(s) 11, 456
SstI GAGCTC 1 cut(s) 690
StyD4I CCNGG 1 cut(s) 1273
SwaI ATTTAAAT 1 cut(s) 1101
TaaI ACNGT 4 cut(s) 211, 277, 895, 1220
TaqI TCGA 2 cut(s) 306, 989
TasI AATT 4 cut(s) 144, 187, 771, 1113
TatI WGTACW 3 cut(s) 68, 532, 1215
TauI GCSGC 1 cut(s) 490
TfiI GAWTC 5 cut(s) 314, 571, 937, 1312, 1319
Tru1I TTAA 8 cut(s) 111, 132, 413, 515, 594, 822, 1052, 1100
Tru9I TTAA 8 cut(s) 111, 132, 413, 515, 594, 822, 1052, 1100
TscAI CASTG 2 cut(s) 214, 679
TseFI GTSAC 2 cut(s) 211, 1257
TseI GCWGC 3 cut(s) 194, 219, 813
Tsp45I GTSAC 2 cut(s) 211, 1257
TspDTI ATGAA 9 cut(s) 580, 638, 800, 869, 950, 1101, 1239, 1321, 1349
TspRI CASTG 2 cut(s) 214, 679
Van91I CCANNNNNTGG 1 cut(s) 391
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 328
VspI ATTAAT 1 cut(s) 594
XapI RAATTY 2 cut(s) 187, 1113
XceI RCATGY 1 cut(s) 1132
XhoI CTCGAG 2 cut(s) 305, 988
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 2 cut(s) 11, 456
ZrmI AGTACT 3 cut(s) 70, 534, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.