Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000649

Physical Location & Seq

Genomic Coordinates Reverse (-)

61079303 .. 61082560

Gene Structure 3258 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ33398

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1188 bp

ATGGCAACCGAAGTACCGGAGCAAGTCCCTGACGACGAAATCAAATCGATGTCGATCGCCGACTCTGAGTCGCAAGACCAGCCCGCAAGCTCGTCCAGTCCATCTGTCCAATTGAGTCTGGATGAGAGGTACAACCTACTTAGGAGCATCGCGGAGGAGTGTATCCTAGAAGAAGAGCTCAAGAATTTGCTGGATAAGAAGGCCGAGCCGATCTGCTACGACGGCTTTGAGCCCTCCGGTAGAATGCATATTGCTCAGGGAGTTTTGAAGGCAATTAATGTGAACAAGATGATTGGCGCAGGCTGCAGAGTGAAGATATGGATTGCCGATTGGTTTGCGCAGCTAAACAATAAGATGGGGGGTGATTTGAACAAGATTGAGACTGTGGGGAAGTATTTTATTGAGATATGGAGGTCTGTGGGGATGAATCTGGACAGTGGGAAAGTTGAGTTTTTGTGGTCTTCTAAGGAGATCAATGCCAGGTCACATGAGTACTGGCCGCGTGTAATGGATATTGCCAGGAAAAACAAGCTTTCGAGGATCATTAGGTGTAGTCAAATTATGGGTCGCACAGATCAAGAAGAGTTAACTGCTGCCCAGATCCTCTACCCGTGTATGCAATGTGCAGATATTTTCTTCCTGCAGGCTGACATCTGTCAACTCGGTATGGATCAACGTAAAGTGAATGTGCTCGCACGAGAGTACTGTGATGACATCAAGAAGAAAAACAAGCCTATTATCTTGTCACACCATATGCTACCTGGTTTACAGCAAGGGCAGGAGAAAATGTCAAAAAGTGATCTATCCTCCTCCATATTTATGGAAGATGAAGAGGCGGAAGTGAATACAAAGATTAAGAAGGCTTACTGCCCTCCAGAAAAGGTCGAAGGCAATCCTTGTTTGGAATACGTACAGTACCTCATTTTCCCTTGGTTTCATGAGTTCACCGTAGAGTGCAGTGAAAAGAACGGTGGAGATATCACTTTCAAAGAGTTTGAAGAATTGAGTGCTGCTTATGCAAAAGGTGAGGTCCATCCCGGAGACCTCAAACCATCTTTGGCAAAAGCATTGAATAGAATACTGGAGCCCGTACGATCCCATTTCAAGAATGACAAAGCTGCCAAACAGCTATTACAAAGTGTAAAGGTACATTTGGTTTTACCTTTGGATAAAATATTATGTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004812 GO:0004831 GO:0005488 GO:0005515 GO:0005524 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006437 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0017076 GO:0019538 GO:0019752 GO:0030554 GO:0031406 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0034660 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043177 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0046483 GO:0046983 GO:0071704 GO:0072545 GO:0090304 GO:0097159 GO:0097367 GO:0140098 GO:0140101 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_1b

Protein Analysis

395

Amino Acids

44.84

Weight (kDa)

5.56

Isoelectric Point (pI)

56.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1b	PF00579	67 - 361	5e-62	tRNA synthetases class I (W and Y)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000649)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33840
fragaria_vesca	FvH4_2g00190 FvH4_4g18133 FvH4_4g36450 FvH4_5g02490 FvH4_5g35150 FvH4_6g35840
malus_domestica	MD04G1034100.v1.1 MD06G1084100.v1.1
prunus_persica	Prupe.2G139100_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1
pyrus_communis	pycom04g02850
rosa_chinensis	RchiOBHm_Chr1g0324941 RchiOBHm_Chr1g0340751 RchiOBHm_Chr2g0133721 RchiOBHm_Chr2g0153921 RchiOBHm_Chr4g0396761 RchiOBHm_Chr4g0414291 RchiOBHm_Chr4g0429871 RchiOBHm_Chr4g0446211 RchiOBHm_Chr5g0057181 RchiOBHm_Chr5g0057391 RchiOBHm_Chr5g0077561 RchiOBHm_Chr6g0307121 RchiOBHm_Chr7g0197611
rosa_laevigata	RLG00000003922 RLG00000005681 RLG00000017313 RLG00000029149 RLG00000035152
rosa_multiflora	Rmu_co8043936.1_g000001 Rmu_sc0001164.1_g000009 Rmu_sc0001334.1_g000004 Rmu_sc0004952.1_g000011 Rmu_sc0013154.1_g000007 Rmu_sc0017650.1_g000006 Rmu_sc0018062.1_g000003
rosa_roxburghii	Rroxscaffold_1G00023030 Rroxscaffold_2G00094650 Rroxscaffold_3G00223000 Rroxscaffold_3G00232150 Rroxscaffold_3G00248030 Rroxscaffold_3G00248040 Rroxscaffold_3G00258320
rosa_rugosa	Rorug04G0184600 Rorug04G0368500 Rorug05G0306000 Rorug05G0307100 Rorug07G0041900
rosa_samantha	Rh2CG164400 Rh2DG389300 Rh3AG306800 Rh4AG430600 Rh4BG440000 Rh4CG457300 Rh4DG438500 Rh5AG375500 Rh5BG386300 Rh5CG409700 Rh5DG400300 Rh6CG481100 Rh7AG166300 Rh7AG360600 Rh7BG168400 Rh7CG174700 Rh7DG168000 Rh7DG387600
rosa_wichuraiana	Rw2G022790 Rw4G036780 Rw5G035320 Rw5G035460 Rw7G014480 Rw7G038030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	339
AccII	CGCG	2 cut(s)	152, 502
AciI	CCGC	4 cut(s)	84, 152, 500, 836
AclWI	GGATC	4 cut(s)	548, 595, 678, 1089
AcoI	YGGCCR	1 cut(s)	497
AcsI	RAATTY	1 cut(s)	184
AfaI	GTAC	8 cut(s)	15, 131, 494, 704, 912, 917, 1092, 1149
AgsI	TTSAA	6 cut(s)	268, 370, 988, 998, 1072, 1105
AhdI	GACNNNNNGTC	1 cut(s)	67
AjnI	CCWGG	3 cut(s)	479, 518, 760
AluBI	AGCT	6 cut(s)	90, 178, 343, 532, 1118, 1129
AluI	AGCT	6 cut(s)	90, 178, 343, 532, 1118, 1129
Alw21I	GWGCWC	2 cut(s)	180, 693
Alw26I	GTCTC	2 cut(s)	374, 1035
AlwI	GGATC	4 cut(s)	548, 595, 678, 1089
AoxI	GGCC	2 cut(s)	201, 497
ApeKI	GCWGC	5 cut(s)	303, 340, 593, 1010, 1118
ApoI	RAATTY	1 cut(s)	184
ArsI	GACNNNNNNTTYG	2 cut(s)	550, 582
AseI	ATTAAT	1 cut(s)	276
AspLEI	GCGC	2 cut(s)	299, 340
AspS9I	GGNCC	1 cut(s)	1030
AsuC2I	CCSGG	1 cut(s)	1038
AsuHPI	GGTGA	3 cut(s)	374, 937, 1037
AvaII	GGWCC	1 cut(s)	1030
BaeI	ACNNNNGTAYC	1 cut(s)	30
BanII	GRGCYC	3 cut(s)	180, 234, 1089
BauI	CACGAG	1 cut(s)	696
BbsI	GAAGAC	1 cut(s)	453
Bbv12I	GWGCWC	2 cut(s)	180, 693
BbvI	GCAGC	5 cut(s)	290, 352, 580, 997, 1105
BccI	CCATC	4 cut(s)	109, 349, 1041, 1060
BceAI	ACGGC	1 cut(s)	238
BcgI	CGANNNNNNTGC	2 cut(s)	317, 351
BciT130I	CCWGG	3 cut(s)	481, 520, 762
BciVI	GTATCC	1 cut(s)	173
BcnI	CCSGG	1 cut(s)	1038
BcoDI	GTCTC	2 cut(s)	374, 1035
BfaI	CTAG	1 cut(s)	167
BfmI	CTRYAG	2 cut(s)	304, 641
BfuI	GTATCC	1 cut(s)	173
BisI	GCNGC	6 cut(s)	304, 341, 500, 594, 1011, 1119
BlsI	GCNGC	6 cut(s)	305, 342, 501, 595, 1012, 1120
BmcAI	AGTACT	2 cut(s)	494, 704
Bme1390I	CCNGG	4 cut(s)	481, 520, 762, 1038
Bme18I	GGWCC	1 cut(s)	1030
BmeRI	GACNNNNNGTC	1 cut(s)	67
BmgT120I	GGNCC	1 cut(s)	1030
BmiI	GGNNCC	1 cut(s)	1086
BmrFI	CCNGG	4 cut(s)	481, 520, 762, 1038
BmsI	GCATC	1 cut(s)	156
BoxI	GACNNNNGTC	1 cut(s)	654
BpiI	GAAGAC	1 cut(s)	453
BpmI	CTGGAG	2 cut(s)	858, 1103
Bpu10I	CCTNAGC	1 cut(s)	255
BpuEI	CTTGAG	1 cut(s)	164
BpuMI	CCSGG	1 cut(s)	1038
Bsa29I	ATCGAT	1 cut(s)	47
BsaAI	YACGTR	1 cut(s)	910
BsaBI	GATNNNNATC	1 cut(s)	53
BsaI	GGTCTC	1 cut(s)	1035
BsaJI	CCNNGG	1 cut(s)	929
BsaWI	WCCGGW	2 cut(s)	16, 236
BsaXI	ACNNNNNCTCC	2 cut(s)	773, 803
Bse1I	ACTGG	3 cut(s)	96, 500, 1086
Bse3DI	GCAATG	1 cut(s)	626
Bse8I	GATNNNNATC	1 cut(s)	53
BseBI	CCWGG	3 cut(s)	481, 520, 762
BseCI	ATCGAT	1 cut(s)	47
BseDI	CCNNGG	1 cut(s)	929
BseGI	GGATG	3 cut(s)	127, 429, 1033
BseJI	GATNNNNATC	1 cut(s)	53
BseMI	GCAATG	1 cut(s)	626
BseMII	CTCAG	2 cut(s)	57, 269
BseNI	ACTGG	3 cut(s)	96, 500, 1086
BseRI	GAGGAG	2 cut(s)	170, 799
BseXI	GCAGC	5 cut(s)	290, 352, 580, 997, 1105
BsgI	GTGCAG	2 cut(s)	645, 976
Bsh1236I	CGCG	2 cut(s)	152, 502
Bsh1285I	CGRYCG	1 cut(s)	57
BshFI	GGCC	2 cut(s)	203, 499
BshVI	ATCGAT	1 cut(s)	47
BsiEI	CGRYCG	1 cut(s)	57
BsiHKAI	GWGCWC	2 cut(s)	180, 693
BsiSI	CCGG	3 cut(s)	17, 237, 1038
BsiWI	CGTACG	1 cut(s)	1090
BslFI	GGGAC	1 cut(s)	11
BsmAI	GTCTC	2 cut(s)	374, 1035
BsmFI	GGGAC	1 cut(s)	11
BsmI	GAATGC	1 cut(s)	249
BsnI	GGCC	2 cut(s)	203, 499
Bso31I	GGTCTC	1 cut(s)	1035
Bsp1286I	GDGCHC	4 cut(s)	180, 234, 693, 1089
Bsp143I	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
BspACI	CCGC	4 cut(s)	84, 152, 500, 836
BspANI	GGCC	2 cut(s)	203, 499
BspCNI	CTCAG	2 cut(s)	58, 268
BspDI	ATCGAT	1 cut(s)	47
BspFNI	CGCG	2 cut(s)	152, 502
BspHI	TCATGA	1 cut(s)	937
BspLI	GGNNCC	1 cut(s)	1086
BspMAI	CTGCAG	2 cut(s)	308, 645
BspPI	GGATC	4 cut(s)	548, 595, 678, 1089
BspQI	GCTCTTC	1 cut(s)	168
BspTNI	GGTCTC	1 cut(s)	1035
BsrDI	GCAATG	1 cut(s)	626
BsrI	ACTGG	3 cut(s)	96, 500, 1086
BssECI	CCNNGG	1 cut(s)	929
BssMI	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
BssSI	CACGAG	1 cut(s)	696
BssT1I	CCWWGG	1 cut(s)	929
Bst2BI	CACGAG	1 cut(s)	696
Bst2UI	CCWGG	3 cut(s)	481, 520, 762
Bst4CI	ACNGT	6 cut(s)	385, 437, 707, 915, 949, 971
Bst6I	CTCTTC	3 cut(s)	168, 576, 825
BstBAI	YACGTR	1 cut(s)	910
BstC8I	GCNNGC	5 cut(s)	84, 88, 301, 645, 693
BstDEI	CTNAG	5 cut(s)	66, 140, 255, 465, 1185
BstF5I	GGATG	3 cut(s)	127, 429, 1033
BstFNI	CGCG	2 cut(s)	152, 502
BstHHI	GCGC	2 cut(s)	299, 340
BstKTI	GATC	9 cut(s)	57, 213, 474, 543, 577, 603, 673, 802, 1097
BstMAI	GTCTC	2 cut(s)	374, 1035
BstMBI	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
BstMCI	CGRYCG	1 cut(s)	57
BstMWI	GCNNNNNNNGC	4 cut(s)	79, 222, 303, 1016
BstNI	CCWGG	3 cut(s)	481, 520, 762
BstPAI	GACNNNNGTC	1 cut(s)	654
BstSCI	CCNGG	4 cut(s)	479, 518, 760, 1036
BstSFI	CTRYAG	2 cut(s)	304, 641
BstSNI	TACGTA	1 cut(s)	910
BstUI	CGCG	2 cut(s)	152, 502
BstV1I	GCAGC	5 cut(s)	290, 352, 580, 997, 1105
BstV2I	GAAGAC	1 cut(s)	453
BstX2I	RGATCY	1 cut(s)	600
BstXI	CCANNNNNNTGG	1 cut(s)	820
BstYI	RGATCY	1 cut(s)	600
Bsu15I	ATCGAT	1 cut(s)	47
BsuI	GTATCC	1 cut(s)	173
BsuRI	GGCC	2 cut(s)	203, 499
BsuTUI	ATCGAT	1 cut(s)	47
BtgZI	GCGATG	1 cut(s)	133
BtsCI	GGATG	3 cut(s)	127, 429, 1033
BtsI	GCAGTG	1 cut(s)	964
BtsIMutI	CAGTG	2 cut(s)	442, 964
Cac8I	GCNNGC	5 cut(s)	84, 88, 301, 645, 693
CciI	TCATGA	1 cut(s)	937
CfoI	GCGC	2 cut(s)	299, 340
Cfr13I	GGNCC	1 cut(s)	1030
ClaI	ATCGAT	1 cut(s)	47
CsiI	ACCWGGT	1 cut(s)	760
Csp6I	GTAC	8 cut(s)	14, 130, 493, 703, 911, 916, 1091, 1148
CviAII	CATG	2 cut(s)	488, 938
CviQI	GTAC	8 cut(s)	14, 130, 493, 703, 911, 916, 1091, 1148
DdeI	CTNAG	5 cut(s)	66, 140, 255, 465, 1185
DpnI	GATC	9 cut(s)	56, 212, 473, 542, 576, 602, 672, 801, 1096
DpnII	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
DriI	GACNNNNNGTC	1 cut(s)	67
EaeI	YGGCCR	1 cut(s)	497
Eam1104I	CTCTTC	3 cut(s)	168, 576, 825
Eam1105I	GACNNNNNGTC	1 cut(s)	67
EarI	CTCTTC	3 cut(s)	168, 576, 825
EciI	GGCGGA	1 cut(s)	851
Ecl136II	GAGCTC	1 cut(s)	178
Eco105I	TACGTA	1 cut(s)	910
Eco130I	CCWWGG	1 cut(s)	929
Eco24I	GRGCYC	3 cut(s)	180, 234, 1089
Eco31I	GGTCTC	1 cut(s)	1035
Eco32I	GATATC	1 cut(s)	979
Eco47I	GGWCC	1 cut(s)	1030
Eco53kI	GAGCTC	1 cut(s)	178
EcoICRI	GAGCTC	1 cut(s)	178
EcoRII	CCWGG	3 cut(s)	479, 518, 760
EcoRV	GATATC	1 cut(s)	979
EcoT14I	CCWWGG	1 cut(s)	929
EcoT22I	ATGCAT	1 cut(s)	249
EcoT38I	GRGCYC	3 cut(s)	180, 234, 1089
ErhI	CCWWGG	1 cut(s)	929
FaeI	CATG	2 cut(s)	491, 941
FaqI	GGGAC	1 cut(s)	11
FatI	CATG	2 cut(s)	487, 937
FauI	CCCGC	1 cut(s)	91
FauNDI	CATATG	1 cut(s)	753
Fnu4HI	GCNGC	6 cut(s)	304, 341, 500, 594, 1011, 1119
FokI	GGATG	3 cut(s)	134, 436, 1020
FriOI	GRGCYC	3 cut(s)	180, 234, 1089
Fsp4HI	GCNGC	6 cut(s)	304, 341, 500, 594, 1011, 1119
FspBI	CTAG	1 cut(s)	167
FspI	TGCGCA	1 cut(s)	339
GlaI	GCGC	2 cut(s)	298, 339
GluI	GCNGC	6 cut(s)	304, 341, 500, 594, 1011, 1119
GsuI	CTGGAG	2 cut(s)	858, 1103
HaeIII	GGCC	2 cut(s)	203, 499
HapII	CCGG	3 cut(s)	17, 237, 1038
HhaI	GCGC	2 cut(s)	299, 340
Hin1II	CATG	2 cut(s)	491, 941
Hin6I	GCGC	2 cut(s)	297, 338
HinP1I	GCGC	2 cut(s)	297, 338
HincII	GTYRAC	2 cut(s)	588, 659
HindII	GTYRAC	2 cut(s)	588, 659
HindIII	AAGCTT	1 cut(s)	530
HinfI	GANTC	4 cut(s)	62, 68, 115, 427
HpaI	GTTAAC	1 cut(s)	588
HpaII	CCGG	3 cut(s)	17, 237, 1038
HphI	GGTGA	3 cut(s)	374, 937, 1037
Hpy166II	GTNNAC	5 cut(s)	283, 588, 659, 767, 945
Hpy188I	TCNGA	1 cut(s)	67
Hpy188III	TCNNGA	8 cut(s)	119, 181, 431, 578, 718, 875, 938, 1105
Hpy8I	GTNNAC	5 cut(s)	283, 588, 659, 767, 945
Hpy99I	CGWCG	2 cut(s)	38, 224
HpyAV	CCTTC	4 cut(s)	193, 262, 853, 881
HpyCH4III	ACNGT	6 cut(s)	385, 437, 707, 915, 949, 971
HpyCH4IV	ACGT	2 cut(s)	676, 909
HpyCH4V	TGCA	7 cut(s)	247, 306, 619, 626, 643, 957, 1019
HpyF10VI	GCNNNNNNNGC	4 cut(s)	79, 222, 303, 1016
HpyF3I	CTNAG	5 cut(s)	66, 140, 255, 465, 1185
HpySE526I	ACGT	2 cut(s)	676, 909
Hsp92II	CATG	2 cut(s)	491, 941
HspAI	GCGC	2 cut(s)	297, 338
KspAI	GTTAAC	1 cut(s)	588
Kzo9I	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
LguI	GCTCTTC	1 cut(s)	168
LmnI	GCTCC	3 cut(s)	19, 144, 1084
Lsp1109I	GCAGC	5 cut(s)	290, 352, 580, 997, 1105
LweI	GCATC	1 cut(s)	156
MabI	ACCWGGT	1 cut(s)	760
MaeI	CTAG	1 cut(s)	167
MaeII	ACGT	2 cut(s)	676, 909
MaeIII	GTNAC	2 cut(s)	483, 744
MalI	GATC	9 cut(s)	56, 212, 473, 542, 576, 602, 672, 801, 1096
MboI	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
MfeI	CAATTG	1 cut(s)	110
MflI	RGATCY	1 cut(s)	600
MhlI	GDGCHC	4 cut(s)	180, 234, 693, 1089
MluCI	AATT	5 cut(s)	110, 184, 273, 558, 1001
MlyI	GAGTC	3 cut(s)	56, 77, 124
Mph1103I	ATGCAT	1 cut(s)	249
MseI	TTAA	3 cut(s)	276, 587, 855
MslI	CAYNNNNRTG	1 cut(s)	818
MspI	CCGG	3 cut(s)	17, 237, 1038
MspR9I	CCNGG	4 cut(s)	481, 520, 762, 1038
MunI	CAATTG	1 cut(s)	110
Mva1269I	GAATGC	1 cut(s)	249
MvaI	CCWGG	3 cut(s)	481, 520, 762
MvnI	CGCG	2 cut(s)	152, 502
MwoI	GCNNNNNNNGC	4 cut(s)	79, 222, 303, 1016
NciI	CCSGG	1 cut(s)	1038
NdeI	CATATG	1 cut(s)	753
NdeII	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
NlaIII	CATG	2 cut(s)	491, 941
NlaIV	GGNNCC	1 cut(s)	1086
NmeAIII	GCCGAG	1 cut(s)	229
NmuCI	GTSAC	2 cut(s)	483, 744
NsbI	TGCGCA	1 cut(s)	339
NsiI	ATGCAT	1 cut(s)	249
PagI	TCATGA	1 cut(s)	937
PciSI	GCTCTTC	1 cut(s)	168
PctI	GAATGC	1 cut(s)	249
PfeI	GAWTC	1 cut(s)	427
Pfl23II	CGTACG	1 cut(s)	1090
PfoI	TCCNGGA	1 cut(s)	1036
PkrI	GCNGC	6 cut(s)	305, 342, 501, 595, 1012, 1120
Ple19I	CGATCG	1 cut(s)	57
PleI	GAGTC	3 cut(s)	56, 76, 123
PpsI	GAGTC	3 cut(s)	56, 76, 123
Ppu21I	YACGTR	1 cut(s)	910
PshAI	GACNNNNGTC	1 cut(s)	654
PshBI	ATTAAT	1 cut(s)	276
Psp124BI	GAGCTC	1 cut(s)	180
Psp6I	CCWGG	3 cut(s)	479, 518, 760
PspGI	CCWGG	3 cut(s)	479, 518, 760
PspLI	CGTACG	1 cut(s)	1090
PspN4I	GGNNCC	1 cut(s)	1086
PspPI	GGNCC	1 cut(s)	1030
PstI	CTGCAG	2 cut(s)	308, 645
PsuI	RGATCY	1 cut(s)	600
PvuI	CGATCG	1 cut(s)	57
RsaI	GTAC	8 cut(s)	15, 131, 494, 704, 912, 917, 1092, 1149
RsaNI	GTAC	8 cut(s)	14, 130, 493, 703, 911, 916, 1091, 1148
RseI	CAYNNNNRTG	1 cut(s)	818
SacI	GAGCTC	1 cut(s)	180
SapI	GCTCTTC	1 cut(s)	168
SaqAI	TTAA	3 cut(s)	276, 587, 855
SatI	GCNGC	6 cut(s)	304, 341, 500, 594, 1011, 1119
Sau3AI	GATC	9 cut(s)	54, 210, 471, 540, 574, 600, 670, 799, 1094
Sau96I	GGNCC	1 cut(s)	1030
SbfI	CCTGCAGG	1 cut(s)	645
ScaI	AGTACT	2 cut(s)	494, 704
SchI	GAGTC	3 cut(s)	56, 77, 124
ScrFI	CCNGG	4 cut(s)	481, 520, 762, 1038
SdaI	CCTGCAGG	1 cut(s)	645
SduI	GDGCHC	4 cut(s)	180, 234, 693, 1089
SexAI	ACCWGGT	1 cut(s)	760
SfaNI	GCATC	1 cut(s)	156
SfcI	CTRYAG	2 cut(s)	304, 641
SinI	GGWCC	1 cut(s)	1030
SmiMI	CAYNNNNRTG	1 cut(s)	818
SmlI	CTYRAG	1 cut(s)	179
SmoI	CTYRAG	1 cut(s)	179
SnaBI	TACGTA	1 cut(s)	910
Sse8387I	CCTGCAGG	1 cut(s)	645
Sse9I	AATT	5 cut(s)	110, 184, 273, 558, 1001
SsiI	CCGC	4 cut(s)	84, 152, 500, 836
SspI	AATATT	1 cut(s)	1176
SspMI	CTAG	1 cut(s)	167
SstI	GAGCTC	1 cut(s)	180
StyD4I	CCNGG	4 cut(s)	479, 518, 760, 1036
StyI	CCWWGG	1 cut(s)	929
TaaI	ACNGT	6 cut(s)	385, 437, 707, 915, 949, 971
TaiI	ACGT	2 cut(s)	679, 912
TaqI	TCGA	4 cut(s)	47, 53, 536, 885
TasI	AATT	5 cut(s)	110, 184, 273, 558, 1001
TatI	WGTACW	2 cut(s)	492, 702
TauI	GCSGC	1 cut(s)	502
TfiI	GAWTC	1 cut(s)	427
Tru1I	TTAA	3 cut(s)	276, 587, 855
Tru9I	TTAA	3 cut(s)	276, 587, 855
TscAI	CASTG	2 cut(s)	442, 964
TseFI	GTSAC	2 cut(s)	483, 744
TseI	GCWGC	5 cut(s)	303, 340, 593, 1010, 1118
Tsp45I	GTSAC	2 cut(s)	483, 744
TspDTI	ATGAA	3 cut(s)	440, 843, 926
TspRI	CASTG	2 cut(s)	442, 964
VpaK11BI	GGWCC	1 cut(s)	1030
VspI	ATTAAT	1 cut(s)	276
XapI	RAATTY	1 cut(s)	184
XcmI	CCANNNNNNNNNTGG	1 cut(s)	758
XspI	CTAG	1 cut(s)	167
ZrmI	AGTACT	2 cut(s)	494, 704
Zsp2I	ATGCAT	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.