RLG00000005681

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000649
Physical Location & Seq
Forward (+)
792109 .. 795117
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005681

Sequence Viewer

Length: 1527 bp
ATGGCAAACCTAGCAATAGTAGACAACAACAATGTCAATGTCAAGACCTCATCAACATCAAATTCAAGTACTACTATCCTCAGCCGGTTACATCTACCTGGTTATGGACTTAAAACAGCTTTGCCGAAGCTTAAGCCCCTGAAAAATATTGTGCCGAGAAGAATAAAGGCCTCAGCAATATCATCAAATTCAGCTGCAACCAAGTTCACTGTCACCAGCTGCTTACCTCTCCAATGTTTCAGGTTCGGACTCAAAGCACCAGAGCCGGATCAAACTGTTGACAATGGATCGCTTGCAACCGCTCTCGAGTCTGATTCGGGCCATGTGGTCCAACCCTTCACCTCAACCAAGTCCACAATCCCCACCTGTTTATCTCTCCCTCTCCAAAGTTTGGGACATTTTCAGGAAAGCTTAAAAACAGAGCTATTATCTAAGCCAAGCAAGACTAATGTAACTAGAAAAGAAGAAGAAGAAGTGTTATCAAAGCAGCCTAATGTGGCAGAAGATTTGATATTAAAAGTAGGCAACAAAGTACTCAAGGAAGGCTCAAAGGGCCAAAGATACTTGTGGAATCTTGCACGACAACAATTCATTAATCGCAAACTCAAGAATACTATCCTAATGAATATACATAACAAGTACCGGATGGTGAGAAGTGTGGGAGAGGATTGCAGTGAAGAAAAAGAGCTCAAGACTTTGATAGCTAATAAGCCTGAACCTATCTGCTTCAACCGCTTTCAACCCTCCGGCCTAATGCACATTGCTGAGGGAATTATGGAGGTGATGAATGTGAACAAGCTGACCAACGCCGGCTGCAGAGTTAAAATCTTGATTGCGGATCAGTTAGCACGCATGAACAAGAAGTTGGGGGGAGACATGGAGAAGATAGAGACTGTCGGACTATATATGATTGAGGTATGGAAGGCAATGGGGATGAATCTCAAAGATGGCAAAGTAGAGATTTTATGGTCTTCAAAGGAGGTTGGCTCGAGAGTAAATGAGTATTTGGATCTTGCTGACATCTGCCAGTTTGGAATGGATAAGCGGGAAGTGAACATTCTTGCAAGAGAGTACTGTGATGAAATCCAGAGAAACAACAAGCCTATTGTCTTGTCACACCATATATTACCCGGTTTGAAGGAAGGGCAGGGGAAAATGTCAAAGAAGATTCCCGATTCTGCCATATTCATGAACGATAATGAGGCTGAAGTAAATCGGAAGATAGAGGAAGCTTTCTGCCCTGAAAAGGTTGTTGAAGGGAATCCATGTTTGGAGTACATTAAGTACCTCATTTTTCCTTGGTTTAATGGATTGGTTGTGGAGCAAATTGAACAGGATGGTGGAGTTAGGACCTTCAAAAGCTTTGAAGATTTGATTGTTCATTATCAATGTGGTATTCTACAACCAGACGACCTTAAAGTGGCTTTATCCAAAGCCATTAATAAAATATTGCAGCCCGTAAGAAACCACTTTGAGGATAATGATACAGCTAAACAGCTCTTACAAAGTGTTTTGCGAAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_1b

Protein Analysis

509

Amino Acids

57.07

Weight (kDa)

8.9

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1b PF00579 240 - 327 1.1e-08 tRNA synthetases class I (W and Y)
tRNA-synt_1b PF00579 339 - 485 2.3e-18 tRNA synthetases class I (W and Y)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000649)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33840
fragaria_vesca FvH4_2g00190 FvH4_4g18133 FvH4_4g36450 FvH4_5g02490 FvH4_5g35150 FvH4_6g35840
malus_domestica MD04G1034100.v1.1 MD06G1084100.v1.1
prunus_persica Prupe.2G139100_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G096900_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1 Prupe.5G100500_v2.0.a1
pyrus_communis pycom04g02850
rosa_chinensis RchiOBHm_Chr1g0324941 RchiOBHm_Chr1g0340751 RchiOBHm_Chr2g0133721 RchiOBHm_Chr2g0153921 RchiOBHm_Chr4g0396761 RchiOBHm_Chr4g0414291 RchiOBHm_Chr4g0429871 RchiOBHm_Chr4g0446211 RchiOBHm_Chr5g0057181 RchiOBHm_Chr5g0057391 RchiOBHm_Chr5g0077561 RchiOBHm_Chr6g0307121 RchiOBHm_Chr7g0197611
rosa_laevigata RLG00000003922 RLG00000005681 RLG00000017313 RLG00000029149 RLG00000035152
rosa_multiflora Rmu_co8043936.1_g000001 Rmu_sc0001164.1_g000009 Rmu_sc0001334.1_g000004 Rmu_sc0004952.1_g000011 Rmu_sc0013154.1_g000007 Rmu_sc0017650.1_g000006 Rmu_sc0018062.1_g000003
rosa_roxburghii Rroxscaffold_1G00023030 Rroxscaffold_2G00094650 Rroxscaffold_3G00223000 Rroxscaffold_3G00232150 Rroxscaffold_3G00248030 Rroxscaffold_3G00248040 Rroxscaffold_3G00258320
rosa_rugosa Rorug04G0184600 Rorug04G0368500 Rorug05G0306000 Rorug05G0307100 Rorug07G0041900
rosa_samantha Rh2CG164400 Rh2DG389300 Rh3AG306800 Rh4AG430600 Rh4BG440000 Rh4CG457300 Rh4DG438500 Rh5AG375500 Rh5BG386300 Rh5CG409700 Rh5DG400300 Rh6CG481100 Rh7AG166300 Rh7AG360600 Rh7BG168400 Rh7CG174700 Rh7DG168000 Rh7DG387600
rosa_wichuraiana Rw2G022790 Rw4G036780 Rw5G035320 Rw5G035460 Rw7G014480 Rw7G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 21
AciI CCGC 4 cut(s) 300, 733, 836, 1045
AclWI GGATC 4 cut(s) 276, 295, 846, 1017
AcsI RAATTY 2 cut(s) 61, 187
AcuI CTGAAG 1 cut(s) 1227
AfaI GTAC 6 cut(s) 70, 534, 641, 1073, 1277, 1286
AfiI CCNNNNNNNGG 5 cut(s) 104, 391, 1246, 1420, 1474
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 9 cut(s) 66, 730, 740, 975, 1138, 1256, 1331, 1357, 1367
AjnI CCWGG 1 cut(s) 97
AjuI GAANNNNNNNTTGG 4 cut(s) 848, 880, 1253, 1285
Alw21I GWGCWC 1 cut(s) 690
Alw26I GTCTC 2 cut(s) 867, 884
AlwI GGATC 4 cut(s) 276, 295, 846, 1017
Ama87I CYCGRG 2 cut(s) 305, 988
AoxI GGCC 4 cut(s) 168, 319, 553, 748
ApeKI GCWGC 5 cut(s) 194, 219, 487, 813, 1453
ApoI RAATTY 2 cut(s) 61, 187
AseI ATTAAT 2 cut(s) 594, 1440
AspS9I GGNCC 4 cut(s) 319, 328, 553, 1350
AsuC2I CCSGG 1 cut(s) 1131
AsuHPI GGTGA 4 cut(s) 205, 331, 661, 793
AvaI CYCGRG 2 cut(s) 305, 988
AvaII GGWCC 2 cut(s) 328, 1350
BanII GRGCYC 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 963
Bbv12I GWGCWC 1 cut(s) 690
BbvCI CCTCAGC 3 cut(s) 80, 172, 765
BbvI GCAGC 5 cut(s) 181, 206, 499, 800, 1465
BccI CCATC 3 cut(s) 640, 941, 1331
BciT130I CCWGG 1 cut(s) 99
BcnI CCSGG 1 cut(s) 1131
BcoDI GTCTC 2 cut(s) 867, 884
BfaI CTAG 2 cut(s) 11, 456
BfmI CTRYAG 1 cut(s) 814
BfrI CTTAAG 1 cut(s) 131
BisI GCNGC 5 cut(s) 195, 220, 488, 814, 1454
BlsI GCNGC 5 cut(s) 196, 221, 489, 815, 1455
BmcAI AGTACT 3 cut(s) 70, 534, 1073
Bme1390I CCNGG 2 cut(s) 99, 1131
Bme18I GGWCC 2 cut(s) 328, 1350
BmeT110I CYCGRG 2 cut(s) 305, 988
BmgT120I GGNCC 4 cut(s) 319, 328, 553, 1350
BmrFI CCNGG 2 cut(s) 99, 1131
BpiI GAAGAC 1 cut(s) 963
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
Bpu10I CCTNAGC 3 cut(s) 80, 172, 765
BpuEI CTTGAG 3 cut(s) 521, 590, 674
BpuMI CCSGG 1 cut(s) 1131
BsaJI CCNNGG 1 cut(s) 1298
BsaWI WCCGGW 1 cut(s) 642
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 391, 1246, 1420, 1474
Bse118I RCCGGY 2 cut(s) 84, 809
Bse1I ACTGG 1 cut(s) 1027
Bse3DI GCAATG 2 cut(s) 759, 933
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 1298
BseGI GGATG 3 cut(s) 651, 939, 1342
BseLI CCNNNNNNNGG 5 cut(s) 104, 391, 1246, 1420, 1474
BseMI GCAATG 2 cut(s) 759, 933
BseMII CTCAG 3 cut(s) 94, 186, 756
BseNI ACTGG 1 cut(s) 1027
BseXI GCAGC 5 cut(s) 181, 206, 499, 800, 1465
BshFI GGCC 4 cut(s) 170, 321, 555, 750
BsiHKAI GWGCWC 1 cut(s) 690
BsiHKCI CYCGRG 2 cut(s) 305, 988
BsiSI CCGG 6 cut(s) 85, 266, 643, 747, 810, 1131
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 5 cut(s) 104, 391, 1246, 1420, 1474
BsmAI GTCTC 2 cut(s) 867, 884
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 4 cut(s) 170, 321, 555, 750
BsoBI CYCGRG 2 cut(s) 305, 988
Bsp1286I GDGCHC 1 cut(s) 690
Bsp143I GATC 4 cut(s) 268, 287, 838, 1009
BspACI CCGC 4 cut(s) 300, 733, 836, 1045
BspANI GGCC 4 cut(s) 170, 321, 555, 750
BspCNI CTCAG 3 cut(s) 93, 185, 757
BspHI TCATGA 1 cut(s) 1188
BspMAI CTGCAG 1 cut(s) 818
BspPI GGATC 4 cut(s) 276, 295, 846, 1017
BspTI CTTAAG 1 cut(s) 131
BsrBI CCGCTC 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 759, 933
BsrFI RCCGGY 2 cut(s) 84, 809
BsrI ACTGG 1 cut(s) 1027
BssAI RCCGGY 2 cut(s) 84, 809
BssECI CCNNGG 1 cut(s) 1298
BssMI GATC 4 cut(s) 268, 287, 838, 1009
BssT1I CCWWGG 1 cut(s) 1298
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 4 cut(s) 211, 277, 895, 1076
Bst6I CTCTTC 1 cut(s) 1513
BstAFI CTTAAG 1 cut(s) 131
BstC8I GCNNGC 3 cut(s) 294, 811, 850
BstDEI CTNAG 4 cut(s) 80, 172, 432, 765
BstF5I GGATG 3 cut(s) 651, 939, 1342
BstKTI GATC 4 cut(s) 271, 290, 841, 1012
BstMAI GTCTC 2 cut(s) 867, 884
BstMBI GATC 4 cut(s) 268, 287, 838, 1009
BstMWI GCNNNNNNNGC 3 cut(s) 11, 552, 732
BstNI CCWGG 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 97, 1129
BstSFI CTRYAG 1 cut(s) 814
BstV1I GCAGC 5 cut(s) 181, 206, 499, 800, 1465
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BsuRI GGCC 4 cut(s) 170, 321, 555, 750
BtsCI GGATG 3 cut(s) 651, 939, 1342
BtsI GCAGTG 1 cut(s) 679
BtsIMutI CAGTG 2 cut(s) 207, 679
Cac8I GCNNGC 3 cut(s) 294, 811, 850
CciI TCATGA 1 cut(s) 1188
Cfr10I RCCGGY 2 cut(s) 84, 809
Cfr13I GGNCC 4 cut(s) 319, 328, 553, 1350
CsiI ACCWGGT 1 cut(s) 97
Csp6I GTAC 6 cut(s) 69, 533, 640, 1072, 1276, 1285
CviAII CATG 5 cut(s) 323, 853, 877, 1189, 1266
CviQI GTAC 6 cut(s) 69, 533, 640, 1072, 1276, 1285
DdeI CTNAG 4 cut(s) 80, 172, 432, 765
DpnI GATC 4 cut(s) 270, 289, 840, 1011
DpnII GATC 4 cut(s) 268, 287, 838, 1009
Eam1104I CTCTTC 1 cut(s) 1513
EarI CTCTTC 1 cut(s) 1513
Ecl136II GAGCTC 1 cut(s) 688
Eco130I CCWWGG 1 cut(s) 1298
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 690
Eco47I GGWCC 2 cut(s) 328, 1350
Eco53kI GAGCTC 1 cut(s) 688
Eco57I CTGAAG 1 cut(s) 1227
Eco88I CYCGRG 2 cut(s) 305, 988
EcoICRI GAGCTC 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 1350
EcoRII CCWGG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 1298
EcoT38I GRGCYC 1 cut(s) 690
ErhI CCWWGG 1 cut(s) 1298
FaeI CATG 5 cut(s) 326, 856, 880, 1192, 1269
FaqI GGGAC 1 cut(s) 408
FatI CATG 5 cut(s) 322, 852, 876, 1188, 1265
FauI CCCGC 1 cut(s) 1038
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 5 cut(s) 195, 220, 488, 814, 1454
FokI GGATG 3 cut(s) 658, 946, 1349
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 5 cut(s) 195, 220, 488, 814, 1454
FspBI CTAG 2 cut(s) 11, 456
GluI GCNGC 5 cut(s) 195, 220, 488, 814, 1454
HaeIII GGCC 4 cut(s) 170, 321, 555, 750
HapII CCGG 6 cut(s) 85, 266, 643, 747, 810, 1131
Hin1II CATG 5 cut(s) 326, 856, 880, 1192, 1269
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HindIII AAGCTT 4 cut(s) 128, 409, 1230, 1360
HinfI GANTC 8 cut(s) 249, 308, 314, 571, 937, 1168, 1175, 1261
HpaII CCGG 6 cut(s) 85, 266, 643, 747, 810, 1131
HphI GGTGA 4 cut(s) 205, 331, 661, 793
Hpy166II GTNNAC 6 cut(s) 22, 207, 280, 354, 793, 1054
Hpy188I TCNGA 4 cut(s) 248, 313, 899, 1218
Hpy8I GTNNAC 6 cut(s) 22, 207, 280, 354, 793, 1054
HpyAV CCTTC 7 cut(s) 346, 536, 916, 1132, 1136, 1250, 1363
HpyCH4III ACNGT 4 cut(s) 211, 277, 895, 1076
HpyCH4V TGCA 8 cut(s) 197, 296, 578, 672, 757, 816, 1064, 1453
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 552, 732
HpyF3I CTNAG 4 cut(s) 80, 172, 432, 765
Hsp92II CATG 5 cut(s) 326, 856, 880, 1192, 1269
KroI GCCGGC 1 cut(s) 809
KroNI GCCGGC 1 cut(s) 811
Kzo9I GATC 4 cut(s) 268, 287, 838, 1009
LmnI GCTCC 1 cut(s) 1321
Lsp1109I GCAGC 5 cut(s) 181, 206, 499, 800, 1465
MabI ACCWGGT 1 cut(s) 97
MaeI CTAG 2 cut(s) 11, 456
MaeIII GTNAC 4 cut(s) 87, 211, 451, 1113
MalI GATC 4 cut(s) 270, 289, 840, 1011
MbiI CCGCTC 1 cut(s) 302
MboI GATC 4 cut(s) 268, 287, 838, 1009
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 690
MluCI AATT 5 cut(s) 61, 187, 587, 771, 1326
MlyI GAGTC 2 cut(s) 243, 317
MmeI TCCRAC 2 cut(s) 355, 877
MroNI GCCGGC 1 cut(s) 809
MslI CAYNNNNRTG 1 cut(s) 1187
MspA1I CMGCKG 2 cut(s) 194, 219
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 6 cut(s) 85, 266, 643, 747, 810, 1131
MspR9I CCNGG 2 cut(s) 99, 1131
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 3 cut(s) 11, 552, 732
NaeI GCCGGC 1 cut(s) 811
NciI CCSGG 1 cut(s) 1131
NdeII GATC 4 cut(s) 268, 287, 838, 1009
NgoMIV GCCGGC 1 cut(s) 809
NlaIII CATG 5 cut(s) 326, 856, 880, 1192, 1269
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 2 cut(s) 211, 1113
PaeR7I CTCGAG 2 cut(s) 305, 988
PagI TCATGA 1 cut(s) 1188
PceI AGGCCT 1 cut(s) 170
PdiI GCCGGC 1 cut(s) 811
PfeI GAWTC 6 cut(s) 314, 571, 937, 1168, 1175, 1261
PflMI CCANNNNNTGG 1 cut(s) 391
PkrI GCNGC 5 cut(s) 196, 221, 489, 815, 1455
PleI GAGTC 2 cut(s) 243, 316
PpsI GAGTC 2 cut(s) 243, 316
PpuMI RGGWCCY 1 cut(s) 1350
PshBI ATTAAT 2 cut(s) 594, 1440
Psp124BI GAGCTC 1 cut(s) 690
Psp5II RGGWCCY 1 cut(s) 1350
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspPI GGNCC 4 cut(s) 319, 328, 553, 1350
PspPPI RGGWCCY 1 cut(s) 1350
PstI CTGCAG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 1009
PvuII CAGCTG 2 cut(s) 194, 219
RsaI GTAC 6 cut(s) 70, 534, 641, 1073, 1277, 1286
RsaNI GTAC 6 cut(s) 69, 533, 640, 1072, 1276, 1285
RseI CAYNNNNRTG 1 cut(s) 1187
SacI GAGCTC 1 cut(s) 690
SatI GCNGC 5 cut(s) 195, 220, 488, 814, 1454
Sau3AI GATC 4 cut(s) 268, 287, 838, 1009
Sau96I GGNCC 4 cut(s) 319, 328, 553, 1350
ScaI AGTACT 3 cut(s) 70, 534, 1073
SchI GAGTC 2 cut(s) 243, 317
ScrFI CCNGG 2 cut(s) 99, 1131
SduI GDGCHC 1 cut(s) 690
SexAI ACCWGGT 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 814
Sfr274I CTCGAG 2 cut(s) 305, 988
SinI GGWCC 2 cut(s) 328, 1350
SlaI CTCGAG 2 cut(s) 305, 988
SmiMI CAYNNNNRTG 1 cut(s) 1187
SmlI CTYRAG 6 cut(s) 131, 305, 536, 605, 689, 988
SmoI CTYRAG 6 cut(s) 131, 305, 536, 605, 689, 988
Sse9I AATT 5 cut(s) 61, 187, 587, 771, 1326
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 4 cut(s) 300, 733, 836, 1045
SspI AATATT 2 cut(s) 148, 1449
SspMI CTAG 2 cut(s) 11, 456
SstI GAGCTC 1 cut(s) 690
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 97, 1129
StyI CCWWGG 1 cut(s) 1298
TaaI ACNGT 4 cut(s) 211, 277, 895, 1076
TaqI TCGA 2 cut(s) 306, 989
TasI AATT 5 cut(s) 61, 187, 587, 771, 1326
TatI WGTACW 4 cut(s) 68, 532, 1071, 1275
TfiI GAWTC 6 cut(s) 314, 571, 937, 1168, 1175, 1261
TscAI CASTG 2 cut(s) 214, 679
TseFI GTSAC 2 cut(s) 211, 1113
TseI GCWGC 5 cut(s) 194, 219, 487, 813, 1453
Tsp45I GTSAC 2 cut(s) 211, 1113
TspDTI ATGAA 9 cut(s) 580, 638, 800, 869, 950, 1095, 1177, 1205, 1370
TspRI CASTG 2 cut(s) 214, 679
Van91I CCANNNNNTGG 1 cut(s) 391
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 328, 1350
VspI ATTAAT 2 cut(s) 594, 1440
XapI RAATTY 2 cut(s) 61, 187
XhoI CTCGAG 2 cut(s) 305, 988
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 2 cut(s) 11, 456
ZrmI AGTACT 3 cut(s) 70, 534, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.