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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 69 |
| AfaI | GTAC | 1 cut(s) | 34 |
| AflIII | ACRYGT | 1 cut(s) | 93 |
| AluBI | AGCT | 2 cut(s) | 51, 61 |
| AluI | AGCT | 2 cut(s) | 51, 61 |
| BbsI | GAAGAC | 1 cut(s) | 30 |
| BpiI | GAAGAC | 1 cut(s) | 30 |
| BstDEI | CTNAG | 1 cut(s) | 80 |
| BstNSI | RCATGY | 1 cut(s) | 97 |
| BstV2I | GAAGAC | 1 cut(s) | 30 |
| Csp6I | GTAC | 1 cut(s) | 33 |
| CviAII | CATG | 1 cut(s) | 94 |
| CviJI | RGCY | 2 cut(s) | 51, 61 |
| CviKI_1 | RGCY | 2 cut(s) | 51, 61 |
| CviQI | GTAC | 1 cut(s) | 33 |
| DdeI | CTNAG | 1 cut(s) | 80 |
| FaeI | CATG | 1 cut(s) | 97 |
| FaiI | YATR | 2 cut(s) | 69, 95 |
| FatI | CATG | 1 cut(s) | 93 |
| FspEI | CC | 4 cut(s) | 15, 92, 96, 97 |
| Hin1II | CATG | 1 cut(s) | 97 |
| HindIII | AAGCTT | 1 cut(s) | 59 |
| Hpy166II | GTNNAC | 1 cut(s) | 99 |
| Hpy188I | TCNGA | 1 cut(s) | 109 |
| Hpy8I | GTNNAC | 1 cut(s) | 99 |
| HpyAV | CCTTC | 1 cut(s) | 104 |
| HpyCH4V | TGCA | 1 cut(s) | 10 |
| HpyF3I | CTNAG | 1 cut(s) | 80 |
| Hsp92II | CATG | 1 cut(s) | 97 |
| MboII | GAAGA | 2 cut(s) | 35, 96 |
| NlaIII | CATG | 1 cut(s) | 97 |
| NspI | RCATGY | 1 cut(s) | 97 |
| PciI | ACATGT | 1 cut(s) | 93 |
| PscI | ACATGT | 1 cut(s) | 93 |
| PsiI | TTATAA | 1 cut(s) | 69 |
| RsaI | GTAC | 1 cut(s) | 34 |
| RsaNI | GTAC | 1 cut(s) | 33 |
| SetI | ASST | 2 cut(s) | 53, 63 |
| SgeI | CNNG | 1 cut(s) | 106 |
| XceI | RCATGY | 1 cut(s) | 97 |