MD06G1219200.v1.1

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000786
Physical Location & Seq
Reverse (-)
35169545 .. 35170327
783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1219200.v1.1.491

Sequence Viewer

Length: 783 bp
ATGCCACCACTTGATGTTTACCTCCTTGTTTCTTTCTCACTCTTCTTCTTGATCGTGAAGTCATATGGAATCAGCAACACTAGTAGTTCTTCATCCTCAACCAATATTTGCGGCACATATGATTGCGGCAACGGCTTAACCTTTCGCTATCCGTTTTGGCATGGCGAAGCCACCACCGCCGATCAGTACTGTGGCTACCCTGGTTTCGGCCTCACATGCTCCGCCGACGGCGAGCCTATCCTCACCCTACCAACCGACTCATATTATGTCAAACAAATAAACTACACTGATTCCACCGTCCATCTTGTTGATATCGATGTCGTTGGCCATACATGCCCTAGGGCACGACATAATGTAACTCTAGGCACCCTCCCCTTAAATTACTCCCATCCAGATTTGAACCTCAGTTTTTACTTCAACTGCACTTCCTACCCTCCTCTCGTTCCTCCCATTGGGTGTTTGGGAGACGGTAAGATGCAGTCTTACGTGTTTACGGTGGGGAACGAGACGGAGGGCTTTGATTGGTTCGAGAACTGCGAGGAGAATGTGGTGGTTCCGGTGATAAAGACAACGGAGATTACTAGTGGCTTTGATGGTTTGATCGGTGGATTTGGTGGCGCTATGAACGAAGGGTTTGTGCTGGACTGGGGCATGGCTAAGGACTGCGGATCTTGTGAGGCAAATGGTGGTTTTTGTGGCTACAACAACACTGCGCACAATTTTCTGTGCTTTTGCAAAAATGGAACCAGAACCAACGGTGTCTGCAAACAAGGTATGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

261

Amino Acids

28.25

Weight (kDa)

4.61

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 104 2.1e-18 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 156 - 248 4.9e-21 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 714
AccB1I GGYRCC 1 cut(s) 365
AciI CCGC 5 cut(s) 111, 126, 177, 222, 666
AclWI GGATC 1 cut(s) 676
AcoI YGGCCR 1 cut(s) 325
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 2 cut(s) 206, 452
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 2 cut(s) 400, 418
AhlI ACTAGT 2 cut(s) 80, 581
AjnI CCWGG 1 cut(s) 199
Alw26I GTCTC 2 cut(s) 459, 500
AlwI GGATC 1 cut(s) 676
AoxI GGCC 2 cut(s) 208, 325
AspA2I CCTAGG 1 cut(s) 338
AspLEI GCGC 2 cut(s) 620, 715
AsuHPI GGTGA 2 cut(s) 235, 571
AvrII CCTAGG 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 346
BalI TGGCCA 1 cut(s) 327
BanI GGYRCC 1 cut(s) 365
BccI CCATC 3 cut(s) 309, 396, 587
BceAI ACGGC 2 cut(s) 148, 244
BciT130I CCWGG 1 cut(s) 201
BcoDI GTCTC 2 cut(s) 459, 500
BcuI ACTAGT 2 cut(s) 80, 581
BfaI CTAG 4 cut(s) 81, 339, 362, 582
BfoI RGCGCY 1 cut(s) 621
BisI GCNGC 2 cut(s) 112, 127
BlnI CCTAGG 1 cut(s) 338
BlsI GCNGC 2 cut(s) 113, 128
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 201
BmiI GGNNCC 3 cut(s) 367, 555, 745
BmrFI CCNGG 1 cut(s) 201
BmrI ACTGGG 1 cut(s) 655
BmsI GCATC 1 cut(s) 465
BmuI ACTGGG 1 cut(s) 655
Bpu10I CCTNAGC 1 cut(s) 657
Bsa29I ATCGAT 1 cut(s) 315
BsaAI YACGTR 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 199, 338
BsaWI WCCGGW 1 cut(s) 556
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 452
Bse1I ACTGG 1 cut(s) 650
BseBI CCWGG 1 cut(s) 201
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 199, 338
BseGI GGATG 2 cut(s) 92, 388
BseLI CCNNNNNNNGG 2 cut(s) 206, 452
BseMII CTCAG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 2 cut(s) 426, 554
BseSI GKGCMC 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 406
BshFI GGCC 2 cut(s) 210, 327
BshNI GGYRCC 1 cut(s) 365
BshVI ATCGAT 1 cut(s) 315
BsiSI CCGG 1 cut(s) 557
BslI CCNNNNNNNGG 2 cut(s) 206, 452
BsmAI GTCTC 2 cut(s) 459, 500
BsmBI CGTCTC 2 cut(s) 459, 500
BsnI GGCC 2 cut(s) 210, 327
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 4 cut(s) 51, 181, 600, 668
BspACI CCGC 5 cut(s) 111, 126, 177, 222, 666
BspANI GGCC 2 cut(s) 210, 327
BspCNI CTCAG 1 cut(s) 417
BspDI ATCGAT 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 367, 555, 745
BspPI GGATC 1 cut(s) 676
BspT107I GGYRCC 1 cut(s) 365
BsrI ACTGG 1 cut(s) 650
BssECI CCNNGG 2 cut(s) 199, 338
BssMI GATC 4 cut(s) 51, 181, 600, 668
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 201
Bst4CI ACNGT 5 cut(s) 191, 298, 470, 496, 758
Bst6I CTCTTC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 2 cut(s) 404, 657
BstF5I GGATG 2 cut(s) 92, 388
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 2 cut(s) 620, 715
BstKTI GATC 4 cut(s) 54, 184, 603, 671
BstMAI GTCTC 2 cut(s) 459, 500
BstMBI GATC 4 cut(s) 51, 181, 600, 668
BstMWI GCNNNNNNNGC 4 cut(s) 132, 176, 216, 333
BstNI CCWGG 1 cut(s) 201
BstNSI RCATGY 2 cut(s) 219, 336
BstSCI CCNGG 1 cut(s) 199
BstSLI GKGCMC 1 cut(s) 346
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 2 cut(s) 210, 327
BsuTUI ATCGAT 1 cut(s) 315
BtsCI GGATG 2 cut(s) 92, 388
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 2 cut(s) 285, 708
Cac8I GCNNGC 1 cut(s) 233
CfoI GCGC 2 cut(s) 620, 715
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 1 cut(s) 187
CviAII CATG 4 cut(s) 161, 216, 333, 652
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 2 cut(s) 404, 657
DpnI GATC 4 cut(s) 53, 183, 602, 670
DpnII GATC 4 cut(s) 51, 181, 600, 668
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 338
Eco32I GATATC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 199
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 338
Esp3I CGTCTC 2 cut(s) 459, 500
FaeI CATG 4 cut(s) 164, 219, 336, 655
FatI CATG 4 cut(s) 160, 215, 332, 651
FauNDI CATATG 2 cut(s) 64, 118
Fnu4HI GCNGC 2 cut(s) 112, 127
FokI GGATG 2 cut(s) 79, 375
Fsp4HI GCNGC 2 cut(s) 112, 127
FspBI CTAG 4 cut(s) 81, 339, 362, 582
FspI TGCGCA 1 cut(s) 714
GlaI GCGC 2 cut(s) 619, 714
GluI GCNGC 2 cut(s) 112, 127
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 2 cut(s) 210, 327
HapII CCGG 1 cut(s) 557
HhaI GCGC 2 cut(s) 620, 715
Hin1II CATG 4 cut(s) 164, 219, 336, 655
Hin6I GCGC 2 cut(s) 618, 713
HinP1I GCGC 2 cut(s) 618, 713
HinfI GANTC 3 cut(s) 69, 257, 290
HpaII CCGG 1 cut(s) 557
HphI GGTGA 2 cut(s) 235, 571
Hpy166II GTNNAC 2 cut(s) 19, 492
Hpy188III TCNNGA 4 cut(s) 49, 55, 392, 529
Hpy8I GTNNAC 2 cut(s) 19, 492
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 623
HpyCH4III ACNGT 5 cut(s) 191, 298, 470, 496, 758
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 4 cut(s) 423, 478, 735, 765
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 176, 216, 333
HpyF3I CTNAG 2 cut(s) 404, 657
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 4 cut(s) 164, 219, 336, 655
HspAI GCGC 2 cut(s) 618, 713
Kzo9I GATC 4 cut(s) 51, 181, 600, 668
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 7 cut(s) 186, 213, 405, 570, 626, 631, 760
LweI GCATC 1 cut(s) 465
MaeI CTAG 4 cut(s) 81, 339, 362, 582
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 355
MalI GATC 4 cut(s) 53, 183, 602, 670
MboI GATC 4 cut(s) 51, 181, 600, 668
MboII GAAGA 3 cut(s) 34, 37, 81
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 1 cut(s) 346
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 2 cut(s) 379, 718
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 1 cut(s) 251
Mox20I TGGCCA 1 cut(s) 327
MscI TGGCCA 1 cut(s) 327
MseI TTAA 2 cut(s) 137, 377
Msp20I TGGCCA 1 cut(s) 327
MspI CCGG 1 cut(s) 557
MspR9I CCNGG 1 cut(s) 201
MvaI CCWGG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 132, 176, 216, 333
NdeI CATATG 2 cut(s) 64, 118
NdeII GATC 4 cut(s) 51, 181, 600, 668
NlaIII CATG 4 cut(s) 164, 219, 336, 655
NlaIV GGNNCC 3 cut(s) 367, 555, 745
NsbI TGCGCA 1 cut(s) 714
NspI RCATGY 2 cut(s) 219, 336
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PfeI GAWTC 2 cut(s) 69, 290
PkrI GCNGC 2 cut(s) 113, 128
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
Ppu21I YACGTR 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 199
PspGI CCWGG 1 cut(s) 199
PspN4I GGNNCC 3 cut(s) 367, 555, 745
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SaqAI TTAA 2 cut(s) 137, 377
SatI GCNGC 2 cut(s) 112, 127
Sau3AI GATC 4 cut(s) 51, 181, 600, 668
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 1 cut(s) 346
SetI ASST 5 cut(s) 24, 143, 405, 489, 775
SfaNI GCATC 1 cut(s) 465
SpeI ACTAGT 2 cut(s) 80, 581
Sse9I AATT 2 cut(s) 379, 718
SsiI CCGC 5 cut(s) 111, 126, 177, 222, 666
SspI AATATT 1 cut(s) 106
SspMI CTAG 4 cut(s) 81, 339, 362, 582
StyD4I CCNGG 1 cut(s) 199
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 5 cut(s) 191, 298, 470, 496, 758
TaiI ACGT 1 cut(s) 489
TaqI TCGA 2 cut(s) 315, 528
TasI AATT 2 cut(s) 379, 718
TatI WGTACW 1 cut(s) 186
TauI GCSGC 2 cut(s) 114, 129
TfiI GAWTC 2 cut(s) 69, 290
Tru1I TTAA 2 cut(s) 137, 377
Tru9I TTAA 2 cut(s) 137, 377
TscAI CASTG 2 cut(s) 292, 715
TspDTI ATGAA 2 cut(s) 81, 638
TspGWI ACGGA 3 cut(s) 141, 524, 587
TspRI CASTG 2 cut(s) 292, 715
XceI RCATGY 2 cut(s) 219, 336
XcmI CCANNNNNNNNNTGG 1 cut(s) 457
XmaJI CCTAGG 1 cut(s) 338
XspI CTAG 4 cut(s) 81, 339, 362, 582
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.