Rh7BG086400

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000786
Physical Location & Seq
Forward (+)
6281359 .. 6285860
4502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG086400.1

Sequence Viewer

Length: 852 bp
ATGTCATCACCTATCTCTTACCTCCTTGCAGCTCTTCTCTCACTCTTGTTATTCAACTTCCATGGAAGCAAAGCCAACTTTGTACCCACAAAAAATTGCCCCATATACAATTGTAGTAATGGCCCTGATTTTCACTACCCTTTTTGGAAAATTGAAGACTCCACCGCCGATCAATATTGTGGTTACCCTGGTTTTGGTCTCAATTGCTCAGAGTCCGGAGAGCCAATACTCAGTCTCCCAAATAATGACTCCTATTTTGTGAAGGACATAAACTTTACTACATCCACCATCACCCTTGTTGACATCGACGTCTTCGATCAACAATGCCCTAAGCCGAGACACAGTATGAGTGTAGGCACGCTCCCATTGGATTATTCCCCATTGGATGGGAACCTGAGCTTCTACTTCAACTGCACTTCCTTTCCTGATCCTGTTGTTGTGCCTCCCATAACCTGCTTGGGAATATGGGGTACCAAAAAGTCTTACGTTTTCAAGGAGGGAGGGCAGCCAGATGGGTTTGATTGGTCTGAAAGTTGTGAGGAGAATGTGGTGGTGACGGTGATGAAGACTGAGGAGATTTATATTATTATTGATGAGTTGACTGGTCATTTTGGAGAGCTTATGAAGCAGGGGTTTGTGCTTAACTGGACCACGGCGAAGGAGTGCGCTTCGTGTGAGACTGGTGGTGGTTTGTGTGGGTACAACCCCACTAAAGCTGATGAGTTTCTCTGCTTCTGCGAGAAGAAAGGCCCTAAGCTCAACCGGAGATTGAAGGTTGTCTTAGGTAATATCTGCAATCCCTTTATTCTTCAGCTGTTTAATGTTCTCCAAGCTCATATATATGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

283

Amino Acids

31.52

Weight (kDa)

4.78

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 101 8.8e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 158 - 248 6.6e-18 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 461
Acc65I GGTACC 1 cut(s) 470
AccB1I GGYRCC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 386
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 1 cut(s) 165
AclWI GGATC 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 794
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 3 cut(s) 84, 472, 701
AfiI CCNNNNNNNGG 2 cut(s) 194, 386
AgsI TTSAA 5 cut(s) 55, 155, 409, 493, 772
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 7 cut(s) 32, 399, 619, 716, 757, 814, 833
AluI AGCT 7 cut(s) 32, 399, 619, 716, 757, 814, 833
Alw26I GTCTC 4 cut(s) 203, 239, 331, 671
AlwI GGATC 1 cut(s) 422
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 2 cut(s) 121, 748
ApeKI GCWGC 2 cut(s) 29, 505
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
Asp718I GGTACC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 3 cut(s) 122, 648, 749
AsuHPI GGTGA 3 cut(s) 283, 565, 571
AvaII GGWCC 1 cut(s) 648
BanI GGYRCC 1 cut(s) 470
BbsI GAAGAC 3 cut(s) 162, 304, 572
BbvI GCAGC 2 cut(s) 41, 517
BccI CCATC 3 cut(s) 296, 380, 506
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 1 cut(s) 189
BcoDI GTCTC 4 cut(s) 203, 239, 331, 671
BfuAI ACCTGC 1 cut(s) 461
BisI GCNGC 2 cut(s) 30, 506
BlsI GCNGC 2 cut(s) 31, 507
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 648
BmgT120I GGNCC 3 cut(s) 122, 648, 749
BmiI GGNNCC 2 cut(s) 392, 472
BmrFI CCNGG 1 cut(s) 189
BpiI GAAGAC 3 cut(s) 162, 304, 572
Bpu10I CCTNAGC 3 cut(s) 330, 395, 753
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 61, 187, 651
BsaWI WCCGGW 2 cut(s) 215, 762
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 386
Bse1I ACTGG 3 cut(s) 607, 650, 685
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 61, 187, 651
BseGI GGATG 2 cut(s) 281, 391
BseLI CCNNNNNNNGG 2 cut(s) 194, 386
BseMII CTCAG 4 cut(s) 222, 244, 386, 561
BseNI ACTGG 3 cut(s) 607, 650, 685
BseRI GAGGAG 2 cut(s) 554, 587
BseXI GCAGC 2 cut(s) 41, 517
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 2 cut(s) 123, 750
BshNI GGYRCC 1 cut(s) 470
BsiSI CCGG 2 cut(s) 216, 763
BslI CCNNNNNNNGG 2 cut(s) 194, 386
BsmAI GTCTC 4 cut(s) 203, 239, 331, 671
BsnI GGCC 2 cut(s) 123, 750
Bso31I GGTCTC 1 cut(s) 203
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 3 cut(s) 169, 316, 427
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 1 cut(s) 165
BspANI GGCC 2 cut(s) 123, 750
BspCNI CTCAG 4 cut(s) 221, 243, 387, 562
BspEI TCCGGA 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 392, 472
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 1 cut(s) 422
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 203
BsrI ACTGG 3 cut(s) 607, 650, 685
BssECI CCNNGG 3 cut(s) 61, 187, 651
BssMI GATC 3 cut(s) 169, 316, 427
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 344, 559
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 7 cut(s) 208, 230, 330, 395, 570, 753, 781
BstDSI CCRYGG 2 cut(s) 61, 651
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 2 cut(s) 281, 391
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 3 cut(s) 172, 319, 430
BstMAI GTCTC 4 cut(s) 203, 239, 331, 671
BstMBI GATC 3 cut(s) 169, 316, 427
BstMWI GCNNNNNNNGC 1 cut(s) 625
BstNI CCWGG 1 cut(s) 189
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 1 cut(s) 187
BstV1I GCAGC 2 cut(s) 41, 517
BstV2I GAAGAC 3 cut(s) 162, 304, 572
BsuRI GGCC 2 cut(s) 123, 750
BtgI CCRYGG 2 cut(s) 61, 651
BtsCI GGATG 2 cut(s) 281, 391
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 668
Cfr13I GGNCC 3 cut(s) 122, 648, 749
Csp6I GTAC 3 cut(s) 83, 471, 700
CviAII CATG 1 cut(s) 62
CviQI GTAC 3 cut(s) 83, 471, 700
DdeI CTNAG 7 cut(s) 208, 230, 330, 395, 570, 753, 781
DpnI GATC 3 cut(s) 171, 318, 429
DpnII GATC 3 cut(s) 169, 316, 427
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 61
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 794
Eco91I GGTNACC 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 749
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 61
FaeI CATG 1 cut(s) 65
FalI AAGNNNNNCTT 2 cut(s) 764, 796
FatI CATG 1 cut(s) 61
Fnu4HI GCNGC 2 cut(s) 30, 506
FokI GGATG 2 cut(s) 268, 398
Fsp4HI GCNGC 2 cut(s) 30, 506
GlaI GCGC 1 cut(s) 667
GluI GCNGC 2 cut(s) 30, 506
HaeIII GGCC 2 cut(s) 123, 750
HapII CCGG 2 cut(s) 216, 763
HhaI GCGC 1 cut(s) 668
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 1 cut(s) 65
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HincII GTYRAC 2 cut(s) 301, 600
HindII GTYRAC 2 cut(s) 301, 600
HinfI GANTC 4 cut(s) 158, 212, 248, 845
HpaII CCGG 2 cut(s) 216, 763
HphI GGTGA 3 cut(s) 283, 565, 571
Hpy166II GTNNAC 2 cut(s) 301, 600
Hpy188I TCNGA 2 cut(s) 211, 529
Hpy188III TCNNGA 2 cut(s) 216, 425
Hpy8I GTNNAC 2 cut(s) 301, 600
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 3 cut(s) 256, 652, 766
HpyCH4III ACNGT 2 cut(s) 344, 559
HpyCH4IV ACGT 2 cut(s) 309, 486
HpyCH4V TGCA 3 cut(s) 29, 414, 795
HpyF10VI GCNNNNNNNGC 1 cut(s) 625
HpyF3I CTNAG 7 cut(s) 208, 230, 330, 395, 570, 753, 781
HpySE526I ACGT 2 cut(s) 309, 486
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 1 cut(s) 65
HspAI GCGC 1 cut(s) 666
Kpn2I TCCGGA 1 cut(s) 215
KpnI GGTACC 1 cut(s) 474
Kzo9I GATC 3 cut(s) 169, 316, 427
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 1 cut(s) 366
Lsp1109I GCAGC 2 cut(s) 41, 517
MaeII ACGT 2 cut(s) 309, 486
MaeIII GTNAC 2 cut(s) 182, 553
MalI GATC 3 cut(s) 171, 318, 429
MboI GATC 3 cut(s) 169, 316, 427
MboII GAAGA 6 cut(s) 26, 167, 304, 577, 754, 800
MfeI CAATTG 2 cut(s) 109, 202
MluCI AATT 4 cut(s) 94, 109, 150, 202
MlyI GAGTC 3 cut(s) 152, 221, 242
MnlI CCTC 6 cut(s) 32, 453, 490, 494, 532, 565
MroI TCCGGA 1 cut(s) 215
MseI TTAA 3 cut(s) 642, 819, 850
MslI CAYNNNNRTG 1 cut(s) 840
MspA1I CMGCKG 1 cut(s) 814
MspI CCGG 2 cut(s) 216, 763
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 2 cut(s) 109, 202
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 1 cut(s) 625
NcoI CCATGG 1 cut(s) 61
NdeII GATC 3 cut(s) 169, 316, 427
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 2 cut(s) 392, 472
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 553
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 1 cut(s) 845
PflMI CCANNNNNTGG 1 cut(s) 386
PkrI GCNGC 2 cut(s) 31, 507
PleI GAGTC 3 cut(s) 152, 220, 242
PpsI GAGTC 3 cut(s) 152, 220, 242
Psp6I CCWGG 1 cut(s) 187
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 392, 472
PspPI GGNCC 3 cut(s) 122, 648, 749
PvuII CAGCTG 1 cut(s) 814
RsaI GTAC 3 cut(s) 84, 472, 701
RsaNI GTAC 3 cut(s) 83, 471, 700
RseI CAYNNNNRTG 1 cut(s) 840
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 3 cut(s) 642, 819, 850
SatI GCNGC 2 cut(s) 30, 506
Sau3AI GATC 3 cut(s) 169, 316, 427
Sau96I GGNCC 3 cut(s) 122, 648, 749
SchI GAGTC 3 cut(s) 152, 221, 242
ScrFI CCNGG 1 cut(s) 189
SinI GGWCC 1 cut(s) 648
SmiMI CAYNNNNRTG 1 cut(s) 840
Sse9I AATT 4 cut(s) 94, 109, 150, 202
SsiI CCGC 1 cut(s) 165
SspI AATATT 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 2 cut(s) 344, 559
TaiI ACGT 2 cut(s) 312, 489
TaqI TCGA 2 cut(s) 306, 315
TasI AATT 4 cut(s) 94, 109, 150, 202
TfiI GAWTC 1 cut(s) 845
Tru1I TTAA 3 cut(s) 642, 819, 850
Tru9I TTAA 3 cut(s) 642, 819, 850
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 2 cut(s) 29, 505
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 2 cut(s) 578, 638
Van91I CCANNNNNTGG 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 648
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.