Rmu_sc0005665.1_g000023

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005665.1
OG0000786
Physical Location & Seq
Reverse (-)
96022 .. 96906
885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005665.1_g000023.1.cds

Sequence Viewer

Length: 885 bp
atgtcatctgctcgtttgtacctccttgcagctcttctctcactcttgttattcaactttcatggaagcaaagcccactttgtaccctcaacaaattgccccatatacaattgcggtaaggatctgaagtttcactaccctttttggaaaattgaagactccaccgccggtcaatattgtggttaccctggttttggtctcaattgcccagagtccggagagccaatactcagtctcccaaataatgactcctaccatgtgaaagacataaactttattgattccaccatcacccttgttgacatcgacgtcttcgatcaacaatgccctagggcaagacacagtattagtgtaggcacgctcccattggattactccccatcggatgtgaacctgagcttctacttcaactgcacttcctttcctgattcagttgtgcctcccataacctgcttgggaatatggggtaccaaaaagtcttacgttttcaaggagggagagcagccaggtgggtttgattggtctgaaagttgtgaggagaatgtggtggtgacggtgatgaagactaaggagctttctcttatcatggatgagttgactggtgattttggagggcttatgaagcaggggtttgtgcttaactggaccacggcgaaggagtgcgcttcgtgtgagagtggtggtggtttgtgtgggtacaacctcactaaagctgatgatgagtttctctgcttctgcaaatgcgagaagaaaggtatgccgtatgctcctttgttccttaatcctttcttaactttaaaatctgcatgggcccagttttggtatgccttctgtttgggtcatattgatgggcttgggtatgttcattgggctggatggctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

294

Amino Acids

32.75

Weight (kDa)

5.01

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 458
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 2 cut(s) 114, 165
AclWI GGATC 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 4 cut(s) 20, 84, 469, 698
AfiI CCNNNNNNNGG 3 cut(s) 194, 215, 819
AgsI TTSAA 4 cut(s) 55, 155, 409, 490
AjnI CCWGG 2 cut(s) 187, 505
AluBI AGCT 4 cut(s) 32, 399, 574, 713
AluI AGCT 4 cut(s) 32, 399, 574, 713
Alw26I GTCTC 2 cut(s) 203, 239
AlwI GGATC 1 cut(s) 129
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 1 cut(s) 810
ApaI GGGCCC 1 cut(s) 814
ApeKI GCWGC 2 cut(s) 29, 502
Asp718I GGTACC 1 cut(s) 467
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 1 cut(s) 665
AspS9I GGNCC 3 cut(s) 645, 810, 811
AsuHPI GGTGA 4 cut(s) 283, 562, 568, 614
AvaII GGWCC 1 cut(s) 645
AvrII CCTAGG 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 814
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 814
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbsI GAAGAC 3 cut(s) 162, 304, 569
BbvI GCAGC 2 cut(s) 41, 514
BccI CCATC 4 cut(s) 296, 388, 842, 870
BceAI ACGGC 2 cut(s) 666, 745
BciT130I CCWGG 2 cut(s) 189, 507
BcoDI GTCTC 2 cut(s) 203, 239
BfaI CTAG 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 458
BisI GCNGC 2 cut(s) 30, 503
BlnI CCTAGG 1 cut(s) 329
BlsI GCNGC 2 cut(s) 31, 504
Bme1390I CCNGG 2 cut(s) 189, 507
Bme18I GGWCC 1 cut(s) 645
BmgT120I GGNCC 3 cut(s) 645, 810, 811
BmiI GGNNCC 2 cut(s) 469, 812
BmrFI CCNGG 2 cut(s) 189, 507
BmrI ACTGGG 1 cut(s) 808
BmuI ACTGGG 1 cut(s) 808
BpiI GAAGAC 3 cut(s) 162, 304, 569
Bpu10I CCTNAGC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 187, 329, 648
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 215, 819
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 3 cut(s) 604, 647, 814
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 2 cut(s) 189, 507
BseDI CCNNGG 3 cut(s) 187, 329, 648
BseGI GGATG 3 cut(s) 391, 595, 881
BseLI CCNNNNNNNGG 3 cut(s) 194, 215, 819
BseMII CTCAG 2 cut(s) 244, 386
BseNI ACTGG 3 cut(s) 604, 647, 814
BseRI GAGGAG 1 cut(s) 551
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 2 cut(s) 41, 514
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 1 cut(s) 812
BshNI GGYRCC 1 cut(s) 467
BsiSI CCGG 2 cut(s) 168, 216
BslI CCNNNNNNNGG 3 cut(s) 194, 215, 819
BsmAI GTCTC 2 cut(s) 203, 239
BsnI GGCC 1 cut(s) 812
Bso31I GGTCTC 1 cut(s) 203
Bsp120I GGGCCC 1 cut(s) 810
Bsp1286I GDGCHC 1 cut(s) 814
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 2 cut(s) 121, 316
BspACI CCGC 2 cut(s) 114, 165
BspANI GGCC 1 cut(s) 812
BspCNI CTCAG 2 cut(s) 243, 387
BspEI TCCGGA 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 469, 812
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 1 cut(s) 129
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 3 cut(s) 604, 647, 814
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 187, 329, 648
BssMI GATC 2 cut(s) 121, 316
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 329
Bst2UI CCWGG 2 cut(s) 189, 507
Bst4CI ACNGT 2 cut(s) 344, 556
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 3 cut(s) 230, 395, 567
BstDSI CCRYGG 1 cut(s) 648
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 3 cut(s) 391, 595, 881
BstHHI GCGC 1 cut(s) 665
BstKTI GATC 2 cut(s) 124, 319
BstMAI GTCTC 2 cut(s) 203, 239
BstMBI GATC 2 cut(s) 121, 316
BstMWI GCNNNNNNNGC 1 cut(s) 622
BstNI CCWGG 2 cut(s) 189, 507
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 187, 505
BstSLI GKGCMC 1 cut(s) 814
BstV1I GCAGC 2 cut(s) 41, 514
BstV2I GAAGAC 3 cut(s) 162, 304, 569
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 1 cut(s) 812
BtgI CCRYGG 1 cut(s) 648
BtsCI GGATG 3 cut(s) 391, 595, 881
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 665
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 3 cut(s) 645, 810, 811
Csp6I GTAC 4 cut(s) 19, 83, 468, 697
CviAII CATG 4 cut(s) 62, 257, 586, 807
CviQI GTAC 4 cut(s) 19, 83, 468, 697
DdeI CTNAG 3 cut(s) 230, 395, 567
DpnI GATC 2 cut(s) 123, 318
DpnII GATC 2 cut(s) 121, 316
DraI TTTAAA 1 cut(s) 798
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 814
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 146
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 187, 505
EcoT14I CCWWGG 1 cut(s) 329
EcoT38I GRGCYC 1 cut(s) 814
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 4 cut(s) 65, 260, 589, 810
FatI CATG 4 cut(s) 61, 256, 585, 806
Fnu4HI GCNGC 2 cut(s) 30, 503
FokI GGATG 2 cut(s) 398, 602
FriOI GRGCYC 1 cut(s) 814
Fsp4HI GCNGC 2 cut(s) 30, 503
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 664
GluI GCNGC 2 cut(s) 30, 503
HaeIII GGCC 1 cut(s) 812
HapII CCGG 2 cut(s) 168, 216
HhaI GCGC 1 cut(s) 665
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 4 cut(s) 65, 260, 589, 810
Hin6I GCGC 1 cut(s) 663
HinP1I GCGC 1 cut(s) 663
HincII GTYRAC 2 cut(s) 301, 597
HindII GTYRAC 2 cut(s) 301, 597
HinfI GANTC 5 cut(s) 158, 212, 248, 281, 428
HpaII CCGG 2 cut(s) 168, 216
HphI GGTGA 4 cut(s) 283, 562, 568, 614
Hpy166II GTNNAC 3 cut(s) 301, 391, 597
Hpy188I TCNGA 3 cut(s) 126, 385, 526
Hpy188III TCNNGA 2 cut(s) 216, 425
Hpy8I GTNNAC 3 cut(s) 301, 391, 597
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 2 cut(s) 649, 838
HpyCH4III ACNGT 2 cut(s) 344, 556
HpyCH4IV ACGT 2 cut(s) 309, 483
HpyCH4V TGCA 4 cut(s) 29, 414, 738, 806
HpyF10VI GCNNNNNNNGC 1 cut(s) 622
HpyF3I CTNAG 3 cut(s) 230, 395, 567
HpySE526I ACGT 2 cut(s) 309, 483
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 4 cut(s) 65, 260, 589, 810
HspAI GCGC 1 cut(s) 663
Kpn2I TCCGGA 1 cut(s) 215
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 2 cut(s) 121, 316
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 366, 571, 772
Lsp1109I GCAGC 2 cut(s) 41, 514
MaeI CTAG 1 cut(s) 330
MaeII ACGT 2 cut(s) 309, 483
MaeIII GTNAC 2 cut(s) 182, 550
MalI GATC 2 cut(s) 123, 318
MboI GATC 2 cut(s) 121, 316
MboII GAAGA 5 cut(s) 26, 167, 304, 574, 760
MfeI CAATTG 2 cut(s) 109, 202
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 814
MluCI AATT 4 cut(s) 94, 109, 150, 202
MlyI GAGTC 3 cut(s) 152, 221, 242
MnlI CCTC 7 cut(s) 32, 97, 450, 487, 529, 605, 713
MroI TCCGGA 1 cut(s) 215
MseI TTAA 4 cut(s) 639, 780, 791, 797
MslI CAYNNNNRTG 1 cut(s) 846
MspI CCGG 2 cut(s) 168, 216
MspR9I CCNGG 2 cut(s) 189, 507
MunI CAATTG 2 cut(s) 109, 202
MvaI CCWGG 2 cut(s) 189, 507
MwoI GCNNNNNNNGC 1 cut(s) 622
NdeII GATC 2 cut(s) 121, 316
NlaIII CATG 4 cut(s) 65, 260, 589, 810
NlaIV GGNNCC 2 cut(s) 469, 812
NmuCI GTSAC 1 cut(s) 550
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfeI GAWTC 2 cut(s) 281, 428
PkrI GCNGC 2 cut(s) 31, 504
PleI GAGTC 3 cut(s) 152, 220, 242
PpsI GAGTC 3 cut(s) 152, 220, 242
Psp6I CCWGG 2 cut(s) 187, 505
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 187, 505
PspN4I GGNNCC 2 cut(s) 469, 812
PspOMI GGGCCC 1 cut(s) 810
PspPI GGNCC 3 cut(s) 645, 810, 811
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 4 cut(s) 20, 84, 469, 698
RsaNI GTAC 4 cut(s) 19, 83, 468, 697
RseI CAYNNNNRTG 1 cut(s) 846
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 4 cut(s) 639, 780, 791, 797
SatI GCNGC 2 cut(s) 30, 503
Sau3AI GATC 2 cut(s) 121, 316
Sau96I GGNCC 3 cut(s) 645, 810, 811
SchI GAGTC 3 cut(s) 152, 221, 242
ScrFI CCNGG 2 cut(s) 189, 507
SduI GDGCHC 1 cut(s) 814
SinI GGWCC 1 cut(s) 645
SmiMI CAYNNNNRTG 1 cut(s) 846
Sse9I AATT 4 cut(s) 94, 109, 150, 202
SsiI CCGC 2 cut(s) 114, 165
SspI AATATT 1 cut(s) 176
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 187, 505
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 2 cut(s) 344, 556
TaiI ACGT 2 cut(s) 312, 486
TaqI TCGA 2 cut(s) 306, 315
TasI AATT 4 cut(s) 94, 109, 150, 202
TfiI GAWTC 2 cut(s) 281, 428
Tru1I TTAA 4 cut(s) 639, 780, 791, 797
Tru9I TTAA 4 cut(s) 639, 780, 791, 797
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 2 cut(s) 29, 502
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 4 cut(s) 50, 575, 635, 854
VpaK11BI GGWCC 1 cut(s) 645
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmaJI CCTAGG 1 cut(s) 329
XspI CTAG 1 cut(s) 330
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.