Rh7AG070300

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000786
Physical Location & Seq
Forward (+)
4936358 .. 4938145
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG070300.1

Sequence Viewer

Length: 819 bp
ATGTCATCACCTACCTCTTACCTCCTCATAGCTCTTCTCTTGCTCTTCTTCTTTAACTTCCATGGAAGCAAAGCCAACTTTTTGGCCTCAACAAATTGCCCCATATACAGATGTGATAATGAGCTTGATTTTCACTACCCTTTTTGGAAAATCGAAGACTCCACAGCCCATCAATATTGTGGATACCCTGGTTTTGGTCTAACATGCTCAGATTCTGGAGAGCCTATACTGACTCTACCAAATGATTCATTCATTGTTAAAGACATAAACTTTACTACATCCACCATCACCCTTGTTGACATTGACGTTGTTAATCAAACATGCCCCAGGGCAAGACACAGTATGAGTGTAGGCACGCTCCCATTGGATTACTCCCCATTGGATGTGAACCTAAGCTTCTACTTCAACTGCACTTCCTTTCCTGATCCTGGTGTGCCTCCCATAACCTGCTTGGGATCGCACAGTACCAAAAAGTCTTATGTGATCACGGAGGGAGAGGAGCCAGATGGGTTTGAGTGGTCGGAATATTGTGCGGAGAATGTGGTGGTGACTGTGAAAAAAACAGAGGAGATTACTAGAAGTATTAGTGAGTTGATTGGTGCATTTGGATTGGCCATGAACAATGGGTTTGTGCTGAACTGGACCATGGCTAAGGAGTGCGGTTCCTGTGAGACTAATGGTGGGTTCTGTGGCTACAACAACTCTGCGCCAGAGATTCTGTGCTTTTGCAAAGATGGTAGTATTGGTACCACAAGCAATGGCCTCTGCAAGAAAGAGAAAGGCAAGATTCGTAACGATCCTGATCTACTTATGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

272

Amino Acids

29.98

Weight (kDa)

4.72

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 100 9.7e-16 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 149 - 246 1.2e-19 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
Acc65I GGTACC 1 cut(s) 746
AccB1I GGYRCC 1 cut(s) 746
AciI CCGC 2 cut(s) 533, 660
AclWI GGATC 3 cut(s) 419, 463, 791
AcoI YGGCCR 1 cut(s) 612
AfaI GTAC 2 cut(s) 466, 748
AfiI CCNNNNNNNGG 2 cut(s) 194, 428
AgsI TTSAA 1 cut(s) 406
AjnI CCWGG 3 cut(s) 187, 326, 427
AluBI AGCT 3 cut(s) 32, 124, 396
AluI AGCT 3 cut(s) 32, 124, 396
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 3 cut(s) 419, 463, 791
AlwNI CAGNNNCTG 1 cut(s) 215
AoxI GGCC 3 cut(s) 84, 612, 760
Asp718I GGTACC 1 cut(s) 746
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 1 cut(s) 642
AsuHPI GGTGA 2 cut(s) 280, 559
AvaII GGWCC 1 cut(s) 642
BaeI ACNNNNGTAYC 2 cut(s) 175, 208
BalI TGGCCA 1 cut(s) 614
BanI GGYRCC 1 cut(s) 746
BbsI GAAGAC 1 cut(s) 162
BccI CCATC 4 cut(s) 177, 293, 500, 728
BciT130I CCWGG 3 cut(s) 189, 328, 429
BciVI GTATCC 1 cut(s) 176
BclI TGATCA 1 cut(s) 483
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 1 cut(s) 576
BfuAI ACCTGC 1 cut(s) 455
BfuI GTATCC 1 cut(s) 176
Bme1390I CCNGG 3 cut(s) 189, 328, 429
Bme18I GGWCC 1 cut(s) 642
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 3 cut(s) 501, 664, 748
BmrFI CCNGG 3 cut(s) 189, 328, 429
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 237
Bpu10I CCTNAGC 2 cut(s) 392, 651
BsaBI GATNNNNATC 1 cut(s) 801
BsaJI CCNNGG 5 cut(s) 61, 187, 326, 327, 645
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 428
Bse1I ACTGG 1 cut(s) 644
Bse3DI GCAATG 1 cut(s) 763
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 3 cut(s) 189, 328, 429
BseDI CCNNGG 5 cut(s) 61, 187, 326, 327, 645
BseGI GGATG 2 cut(s) 278, 388
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 2 cut(s) 194, 428
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 644
BseRI GAGGAG 3 cut(s) 14, 512, 581
BsgI GTGCAG 1 cut(s) 394
BshFI GGCC 3 cut(s) 86, 614, 762
BshNI GGYRCC 1 cut(s) 746
BslI CCNNNNNNNGG 2 cut(s) 194, 428
BsmAI GTCTC 1 cut(s) 665
BsnI GGCC 3 cut(s) 86, 614, 762
Bsp143I GATC 5 cut(s) 424, 455, 483, 796, 802
Bsp19I CCATGG 2 cut(s) 61, 645
BspACI CCGC 2 cut(s) 533, 660
BspANI GGCC 3 cut(s) 86, 614, 762
BspCNI CTCAG 1 cut(s) 221
BspLI GGNNCC 3 cut(s) 501, 664, 748
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 3 cut(s) 419, 463, 791
BspQI GCTCTTC 2 cut(s) 39, 50
BspT107I GGYRCC 1 cut(s) 746
BsrDI GCAATG 1 cut(s) 763
BsrI ACTGG 1 cut(s) 644
BssECI CCNNGG 5 cut(s) 61, 187, 326, 327, 645
BssMI GATC 5 cut(s) 424, 455, 483, 796, 802
BssT1I CCWWGG 2 cut(s) 61, 645
Bst2UI CCWGG 3 cut(s) 189, 328, 429
Bst4CI ACNGT 3 cut(s) 341, 464, 553
Bst6I CTCTTC 2 cut(s) 39, 50
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 3 cut(s) 208, 392, 651
BstDSI CCRYGG 2 cut(s) 61, 645
BstF5I GGATG 2 cut(s) 278, 388
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 5 cut(s) 427, 458, 486, 799, 805
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 5 cut(s) 424, 455, 483, 796, 802
BstNI CCWGG 3 cut(s) 189, 328, 429
BstNSI RCATGY 2 cut(s) 207, 324
BstSCI CCNGG 3 cut(s) 187, 326, 427
BstV2I GAAGAC 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 82
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 3 cut(s) 86, 614, 762
BtgI CCRYGG 2 cut(s) 61, 645
BtsCI GGATG 2 cut(s) 278, 388
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 356
CaiI CAGNNNCTG 1 cut(s) 215
CfoI GCGC 1 cut(s) 709
Cfr13I GGNCC 1 cut(s) 642
Csp6I GTAC 2 cut(s) 465, 747
CviAII CATG 5 cut(s) 62, 204, 321, 616, 646
CviQI GTAC 2 cut(s) 465, 747
DdeI CTNAG 3 cut(s) 208, 392, 651
DpnI GATC 5 cut(s) 426, 457, 485, 798, 804
DpnII GATC 5 cut(s) 424, 455, 483, 796, 802
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 2 cut(s) 39, 50
EarI CTCTTC 2 cut(s) 39, 50
Eco130I CCWWGG 2 cut(s) 61, 645
Eco47I GGWCC 1 cut(s) 642
EcoRII CCWGG 3 cut(s) 187, 326, 427
EcoT14I CCWWGG 2 cut(s) 61, 645
ErhI CCWWGG 2 cut(s) 61, 645
FaeI CATG 5 cut(s) 65, 207, 324, 619, 649
FatI CATG 5 cut(s) 61, 203, 320, 615, 645
FbaI TGATCA 1 cut(s) 483
FokI GGATG 2 cut(s) 265, 395
FspBI CTAG 1 cut(s) 576
GlaI GCGC 1 cut(s) 708
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 3 cut(s) 86, 614, 762
HhaI GCGC 1 cut(s) 709
Hin1II CATG 5 cut(s) 65, 207, 324, 619, 649
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 394
HinfI GANTC 6 cut(s) 158, 212, 232, 245, 715, 787
HphI GGTGA 2 cut(s) 280, 559
Hpy166II GTNNAC 2 cut(s) 298, 388
Hpy188I TCNGA 2 cut(s) 211, 523
Hpy188III TCNNGA 3 cut(s) 216, 422, 800
Hpy8I GTNNAC 2 cut(s) 298, 388
HpyCH4III ACNGT 3 cut(s) 341, 464, 553
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 4 cut(s) 411, 602, 729, 768
HpyF3I CTNAG 3 cut(s) 208, 392, 651
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 5 cut(s) 65, 207, 324, 619, 649
HspAI GCGC 1 cut(s) 707
KpnI GGTACC 1 cut(s) 750
Ksp22I TGATCA 1 cut(s) 483
Kzo9I GATC 5 cut(s) 424, 455, 483, 796, 802
LguI GCTCTTC 2 cut(s) 39, 50
LmnI GCTCC 2 cut(s) 363, 499
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 547, 791
MalI GATC 5 cut(s) 426, 457, 485, 798, 804
MboI GATC 5 cut(s) 424, 455, 483, 796, 802
MboII GAAGA 4 cut(s) 26, 37, 40, 167
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 1 cut(s) 94
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 2 cut(s) 152, 226
MmeI TCCRAC 1 cut(s) 501
MnlI CCTC 9 cut(s) 25, 32, 35, 97, 447, 484, 490, 559, 773
Mox20I TGGCCA 1 cut(s) 614
MscI TGGCCA 1 cut(s) 614
MseI TTAA 3 cut(s) 54, 258, 312
Msp20I TGGCCA 1 cut(s) 614
MspR9I CCNGG 3 cut(s) 189, 328, 429
MvaI CCWGG 3 cut(s) 189, 328, 429
NcoI CCATGG 2 cut(s) 61, 645
NdeII GATC 5 cut(s) 424, 455, 483, 796, 802
NlaIII CATG 5 cut(s) 65, 207, 324, 619, 649
NlaIV GGNNCC 3 cut(s) 501, 664, 748
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 2 cut(s) 207, 324
PasI CCCWGGG 1 cut(s) 327
PciSI GCTCTTC 2 cut(s) 39, 50
PfeI GAWTC 4 cut(s) 212, 245, 715, 787
PleI GAGTC 2 cut(s) 152, 226
PpsI GAGTC 2 cut(s) 152, 226
Psp6I CCWGG 3 cut(s) 187, 326, 427
PspGI CCWGG 3 cut(s) 187, 326, 427
PspN4I GGNNCC 3 cut(s) 501, 664, 748
PspPI GGNCC 1 cut(s) 642
PstNI CAGNNNCTG 1 cut(s) 215
RsaI GTAC 2 cut(s) 466, 748
RsaNI GTAC 2 cut(s) 465, 747
SapI GCTCTTC 2 cut(s) 39, 50
SaqAI TTAA 3 cut(s) 54, 258, 312
Sau3AI GATC 5 cut(s) 424, 455, 483, 796, 802
Sau96I GGNCC 1 cut(s) 642
SchI GAGTC 2 cut(s) 152, 226
ScrFI CCNGG 3 cut(s) 189, 328, 429
SetI ASST 9 cut(s) 13, 17, 24, 34, 126, 309, 393, 398, 449
SinI GGWCC 1 cut(s) 642
Sse9I AATT 1 cut(s) 94
SsiI CCGC 2 cut(s) 533, 660
SspI AATATT 2 cut(s) 176, 527
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 187, 326, 427
StyI CCWWGG 2 cut(s) 61, 645
TaaI ACNGT 3 cut(s) 341, 464, 553
TaiI ACGT 1 cut(s) 309
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 94
TfiI GAWTC 4 cut(s) 212, 245, 715, 787
Tru1I TTAA 3 cut(s) 54, 258, 312
Tru9I TTAA 3 cut(s) 54, 258, 312
TseFI GTSAC 1 cut(s) 547
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 237, 241, 632
TspGWI ACGGA 1 cut(s) 503
VpaK11BI GGWCC 1 cut(s) 642
XceI RCATGY 2 cut(s) 207, 324
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 448
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.