Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000786

Physical Location & Seq

Genomic Coordinates Reverse (-)

31027847 .. 31033077

Gene Structure 5231 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1228700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGCCTACCCATTTTCTCCTAAAACTACCCATCTCCTTCCTCATCATCTTCCACATTTTGATCACAGTAATATTACTCCCCACCGCCGCTTTCGGAGAGGATGATCACCGATACACCGAGTGCCGCAGCTACTACGACTGCGGCCTCCTCAAAAACATCTCCTACCCCTTCTGGGCCACCGCCAGCCGCCCCCAACACTGCGGCCGCGAAGACTACGAGCTCACCTGCCGAGGAGATCAATACCCTGTCATGAAAATTAAAGACCAGGATTTTCTTGTGTTGAACTTTATCGGACAGTTTTACACCATGACAATCGCTCGCTCAGATCTCTGGGACACCGCCTGTATTACCAACATTGTCAACACCACTTTGGACTACGACCGTTTTTCCTACGTCCCAGCTGTTCGGAACCTGTCGCTGTTCTACGGCTGCCCACCGCAGAGCGAGTCAGTACTCAATAACTTCACTTGCAAGGTAGAGGGTACGGACCACAATATATCATACTATGTCGACGACTCTTTGTCGAGGATTCGTCTGAAAAATTGGGCCTCGTGCCATACGAATATTCGAGTTCCGATTATGTGGGAGGGTGTGGATGCTATGCCGCTGGAGAATGTGACGACGGTTGTGCTCAAAGAGGTTTTGAAACAGGGGTTTCAGGTGGCCTACGATGCGGAGTGGGAGCTCTGTAATCGATGTTTAATATCCAATGGAACCTGTGGGTCGAACTCGAGTAATGATTCTTTTCTATGCTTGTGTGGGGACGGACCTCATGATCAGACGTGCCCAGTTGTTGATGACAATCCTTGGAACTGGAAGAGGAAGGTTATTGTAGGTGTTTGCACAGCTACTGCTACCGTTGCTATAATGTGTCTTATTTTCTTTGTATACCAGCGCCGAAACAGAAAACCATATGATCCATCGTCCTTTGTAACTCGAGGCATCCTTTCGAGTACCTATTCTATGGATGACGATATGGAGAAGGGAAGTACCTACCTTGGAGTACATATTTTCAGCTATAAGGAACTTGAAGAAGCGACTAATTATTTTGATTCTGCGAAAGAACTTGGCGATGGAGGCTTTGGCACAGTTTATCATGGAAATGTGCACGATGGGAGAGCGGTTGCAGTCAAGCGTCTATATGAAAACAATTGGAAGAGAGTTGAGCAGTTCATGAATGAAATCGAGATTTTAGCTCGCCTGCGCCACCAAAATCTTGTGTTGCTCTATGGTTGCACCTCTCGACAAAGCCGTGAACTCCTCCTTGTGTACGAGTACATTCCTAACGGAACTCTTGCTGAACATCTTCACGGAGGAAGAGCAAAACCTGGCGCACTTCCATGGCTTACTCGAATAAACATTGCCATTGAAACCGCAAGCGCACTGTCATATCTTCATGCATCTGACATTATCCACCGTGACGTGAAAACTACGAATATTCTTCTTGACAACAACTTCGGTGTTAAGGTAGCAGATTTTGGACTATCTCGCCTCTTCCCAATGAATGTCACGCACATCTCAACTGCTCCACAAGGGACTCCGGGTTATGTTGATCCCGAGTATAACCAATGCTACCAGCTTACGAGTAAGAGTGATGTCTATAGCTTTGGTGTGGTACTGATTGAGCTCATATCATCCATGCCGGCTGTTGACATCACGAGGCATCGACACGAGATCAATTTGTCTACCATGGCGATCAACAAGATTAAAAACCATACATTACATGAGCTTGTAGACCCATACTTAGGGTTTGAATCGGACTCCAGAACACGAAAAATGATCATTGCTGTGGCGGAACTAGCATTTCGCTGCCTGCAGACTGACAAAGACGTGAGACCTTCTATGGTTGAGGTGCTTAATGAACTGAAGTGGATACAGAGCGACGATTTTAACATACAAAAAGCAGAGGAGATTGATATTTTGGCCGATGATATTGTGCCGTTAAAGAGTGATCCGCTGCCACCTTCACCAGATTCCGTGGCATTGAACTTGACTAGCGCGTCTACGACACCAAATGGCAGCGGTAAACTTATTCGTTCTCCGCCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010228 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0031668 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033993 GO:0036211 GO:0042221 GO:0042631 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071462 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0104004 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

683

Amino Acids

77.1

Weight (kDa)

5.63

Isoelectric Point (pI)

44.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	41 - 106	5.9e-11	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	164 - 256	2.8e-15	Wall-associated receptor kinase C-terminal
Pkinase	PF00069	352 - 621	2e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	353 - 622	8.8e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18390 AT1G18390
fragaria_vesca	FvH4_5g11760 FvH4_5g11760 FvH4_5g11790
malus_domestica	MD06G1218900.v1.1 MD06G1219200.v1.1 MD14G1228700.v1.1
prunus_persica	Prupe.5G223900_v2.0.a1 Prupe.5G223900_v2.0.a1 Prupe.5G223900_v2.0.a1
pyrus_communis	pycom06g19560 pycom06g19580 pycom14g19000
rosa_chinensis	RchiOBHm_Chr7g0185891 RchiOBHm_Chr7g0185941 RchiOBHm_Chr7g0185971 RchiOBHm_Chr7g0186001 RchiOBHm_Chr7g0186021 RchiOBHm_Chr7g0209991 RchiOBHm_Chr7g0210001
rosa_laevigata	RLG00000003071 RLG00000004872 RLG00000004873 RLG00000004875 RLG00000005413
rosa_multiflora	Rmu_co8007212.1_g000001 Rmu_co8076798.1_g000001 Rmu_sc0000194.1_g000013 Rmu_sc0000194.1_g000017 Rmu_sc0000194.1_g000019 Rmu_sc0004509.1_g000017 Rmu_sc0004509.1_g000018 Rmu_sc0005665.1_g000002 Rmu_sc0005665.1_g000004 Rmu_sc0005665.1_g000020 Rmu_sc0005665.1_g000023 Rmu_sc0005665.1_g000035
rosa_roxburghii	Rroxscaffold_1G00039510 Rroxscaffold_3G00248670 Rroxscaffold_3G00257880 Rroxscaffold_3G00268500 Rroxscaffold_3G00268510 Rroxscaffold_3G00268530 Rroxscaffold_3G00268580
rosa_rugosa	Rorug06G0471800 Rorug07G0117900
rosa_samantha	Rh1BG104700 Rh4CG290300 Rh7AG070300 Rh7AG070400 Rh7AG070600 Rh7AG251700 Rh7BG086300 Rh7BG086400 Rh7BG246300 Rh7CG077400 Rh7CG077800 Rh7CG078000 Rh7CG268500 Rh7DG077400 Rh7DG077600 Rh7DG077900 Rh7DG078000 Rh7DG258500
rosa_wichuraiana	Rw7G006440 Rw7G006470 Rw7G006480 Rw7G021370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	233
AasI	GACNNNNNNGTC	1 cut(s)	1999
Acc36I	ACCTGC	1 cut(s)	233
AccBSI	CCGCTC	1 cut(s)	1121
AccI	GTMKAC	5 cut(s)	510, 888, 1685, 1734, 2003
AccII	CGCG	2 cut(s)	207, 2000
AclWI	GGATC	3 cut(s)	911, 1547, 1946
AcoI	YGGCCR	2 cut(s)	202, 1923
AcuI	CTGAAG	1 cut(s)	1886
AdeI	CACNNNGTG	1 cut(s)	120
AfaI	GTAC	8 cut(s)	453, 484, 955, 991, 1005, 1271, 1277, 1617
AfiI	CCNNNNNNNGG	2 cut(s)	172, 1745
AgsI	TTSAA	6 cut(s)	283, 646, 1031, 1370, 1754, 1987
AhdI	GACNNNNNGTC	1 cut(s)	520
AjiI	CACGTC	3 cut(s)	783, 1423, 1831
AjnI	CCWGG	2 cut(s)	264, 1327
Alw21I	GWGCWC	5 cut(s)	222, 633, 687, 1110, 1629
Alw26I	GTCTC	1 cut(s)	1828
Alw44I	GTGCAC	1 cut(s)	1106
AlwI	GGATC	3 cut(s)	911, 1547, 1946
AlwNI	CAGNNNCTG	1 cut(s)	851
Ama87I	CYCGRG	3 cut(s)	730, 936, 1556
AoxI	GGCC	6 cut(s)	142, 174, 202, 546, 663, 1923
ApaLI	GTGCAC	1 cut(s)	1106
ApeKI	GCWGC	5 cut(s)	126, 429, 1809, 1957, 2019
ArsI	GACNNNNNNTTYG	2 cut(s)	1439, 1471
Asp700I	GAANNNNTTC	1 cut(s)	1305
AspLEI	GCGC	5 cut(s)	897, 1206, 1334, 1382, 2000
AspS9I	GGNCC	4 cut(s)	174, 487, 546, 767
AsuC2I	CCSGG	1 cut(s)	1542
AsuHPI	GGTGA	3 cut(s)	98, 214, 1959
AvaI	CYCGRG	3 cut(s)	730, 936, 1556
AvaII	GGWCC	2 cut(s)	487, 767
BaeGI	GKGCMC	2 cut(s)	788, 1110
BanII	GRGCYC	3 cut(s)	222, 687, 1629
BauI	CACGAG	3 cut(s)	550, 1657, 1670
BbsI	GAAGAC	1 cut(s)	216
Bbv12I	GWGCWC	5 cut(s)	222, 633, 687, 1110, 1629
BbvI	GCAGC	5 cut(s)	138, 416, 1796, 1944, 2031
BccI	CCATC	4 cut(s)	38, 928, 1067, 1106
BceAI	ACGGC	4 cut(s)	442, 1236, 1924, 2029
BcgI	CGANNNNNNTGC	2 cut(s)	610, 644
BciT130I	CCWGG	2 cut(s)	266, 1329
BciVI	GTATCC	1 cut(s)	1866
BclI	TGATCA	4 cut(s)	60, 103, 775, 1779
BcnI	CCSGG	1 cut(s)	1542
BcoDI	GTCTC	1 cut(s)	1828
BfaI	CTAG	2 cut(s)	1799, 1995
BfmI	CTRYAG	2 cut(s)	1600, 1814
BfoI	RGCGCY	1 cut(s)	898
BfuAI	ACCTGC	1 cut(s)	233
BfuI	GTATCC	1 cut(s)	1866
BglII	AGATCT	1 cut(s)	325
BmcAI	AGTACT	1 cut(s)	453
Bme1390I	CCNGG	3 cut(s)	266, 1329, 1542
Bme18I	GGWCC	2 cut(s)	487, 767
BmeRI	GACNNNNNGTC	1 cut(s)	520
BmeT110I	CYCGRG	3 cut(s)	730, 936, 1556
BmgBI	CACGTC	3 cut(s)	783, 1423, 1831
BmgT120I	GGNCC	4 cut(s)	174, 487, 546, 767
BmiI	GGNNCC	2 cut(s)	410, 715
BmrFI	CCNGG	3 cut(s)	266, 1329, 1542
BmrI	ACTGGG	1 cut(s)	782
BmsI	GCATC	5 cut(s)	586, 661, 951, 1409, 1672
BmuI	ACTGGG	1 cut(s)	782
BpiI	GAAGAC	1 cut(s)	216
BpmI	CTGGAG	2 cut(s)	629, 1747
BpuMI	CCSGG	1 cut(s)	1542
Bsa29I	ATCGAT	1 cut(s)	694
BsaBI	GATNNNNATC	1 cut(s)	801
BsaI	GGTCTC	1 cut(s)	1828
BsaJI	CCNNGG	6 cut(s)	229, 806, 997, 1340, 1689, 1977
Bsc4I	CCNNNNNNNGG	2 cut(s)	172, 1745
Bse118I	RCCGGY	1 cut(s)	1642
Bse1I	ACTGG	2 cut(s)	788, 818
Bse3DI	GCAATG	2 cut(s)	1359, 1782
Bse8I	GATNNNNATC	1 cut(s)	801
BseBI	CCWGG	2 cut(s)	266, 1329
BseCI	ATCGAT	1 cut(s)	694
BseDI	CCNNGG	6 cut(s)	229, 806, 997, 1340, 1689, 1977
BseGI	GGATG	5 cut(s)	106, 601, 942, 973, 1634
BseJI	GATNNNNATC	1 cut(s)	801
BseLI	CCNNNNNNNGG	2 cut(s)	172, 1745
BseMI	GCAATG	2 cut(s)	1359, 1782
BseMII	CTCAG	1 cut(s)	336
BseNI	ACTGG	2 cut(s)	788, 818
BseRI	GAGGAG	4 cut(s)	137, 246, 1250, 1922
BseSI	GKGCMC	2 cut(s)	788, 1110
BseX3I	CGGCCG	1 cut(s)	202
BseXI	GCAGC	5 cut(s)	138, 416, 1796, 1944, 2031
BseYI	CCCAGC	1 cut(s)	397
Bsh1236I	CGCG	2 cut(s)	207, 2000
Bsh1285I	CGRYCG	2 cut(s)	205, 382
BshFI	GGCC	6 cut(s)	144, 176, 204, 548, 665, 1925
BshVI	ATCGAT	1 cut(s)	694
BsiEI	CGRYCG	2 cut(s)	205, 382
BsiHKAI	GWGCWC	5 cut(s)	222, 633, 687, 1110, 1629
BsiHKCI	CYCGRG	3 cut(s)	730, 936, 1556
BsiSI	CCGG	2 cut(s)	1541, 1643
BslFI	GGGAC	4 cut(s)	347, 380, 776, 1549
BslI	CCNNNNNNNGG	2 cut(s)	172, 1745
BsmAI	GTCTC	1 cut(s)	1828
BsmFI	GGGAC	4 cut(s)	347, 380, 776, 1549
BsnI	GGCC	6 cut(s)	144, 176, 204, 548, 665, 1925
Bso31I	GGTCTC	1 cut(s)	1828
BsoBI	CYCGRG	3 cut(s)	730, 936, 1556
Bsp1286I	GDGCHC	6 cut(s)	222, 633, 687, 788, 1110, 1629
Bsp19I	CCATGG	2 cut(s)	1340, 1689
BspANI	GGCC	6 cut(s)	144, 176, 204, 548, 665, 1925
BspCNI	CTCAG	1 cut(s)	335
BspDI	ATCGAT	1 cut(s)	694
BspFNI	CGCG	2 cut(s)	207, 2000
BspHI	TCATGA	3 cut(s)	249, 772, 1173
BspLI	GGNNCC	2 cut(s)	410, 715
BspMAI	CTGCAG	1 cut(s)	1818
BspMI	ACCTGC	1 cut(s)	233
BspPI	GGATC	3 cut(s)	911, 1547, 1946
BspQI	GCTCTTC	1 cut(s)	1312
BspTNI	GGTCTC	1 cut(s)	1828
BsrBI	CCGCTC	1 cut(s)	1121
BsrDI	GCAATG	2 cut(s)	1359, 1782
BsrFI	RCCGGY	1 cut(s)	1642
BsrI	ACTGG	2 cut(s)	788, 818
BssAI	RCCGGY	1 cut(s)	1642
BssECI	CCNNGG	6 cut(s)	229, 806, 997, 1340, 1689, 1977
BssNAI	GTATAC	1 cut(s)	889
BssSI	CACGAG	3 cut(s)	550, 1657, 1670
BssT1I	CCWWGG	4 cut(s)	806, 997, 1340, 1689
Bst1107I	GTATAC	1 cut(s)	889
Bst2BI	CACGAG	3 cut(s)	550, 1657, 1670
Bst2UI	CCWGG	2 cut(s)	266, 1329
Bst4CI	ACNGT	8 cut(s)	67, 297, 383, 625, 859, 1090, 1386, 1418
Bst6I	CTCTTC	4 cut(s)	812, 1151, 1312, 1499
BstC8I	GCNNGC	7 cut(s)	184, 319, 1198, 1202, 1378, 1644, 1814
BstDEI	CTNAG	2 cut(s)	322, 1744
BstDSI	CCRYGG	3 cut(s)	1340, 1689, 1977
BstF5I	GGATG	5 cut(s)	106, 601, 942, 973, 1634
BstFNI	CGCG	2 cut(s)	207, 2000
BstH2I	RGCGCY	1 cut(s)	898
BstHHI	GCGC	5 cut(s)	897, 1206, 1334, 1382, 2000
BstMAI	GTCTC	1 cut(s)	1828
BstMCI	CGRYCG	2 cut(s)	205, 382
BstMWI	GCNNNNNNNGC	4 cut(s)	671, 860, 1077, 1799
BstNI	CCWGG	2 cut(s)	266, 1329
BstSCI	CCNGG	3 cut(s)	264, 1327, 1540
BstSFI	CTRYAG	2 cut(s)	1600, 1814
BstSLI	GKGCMC	2 cut(s)	788, 1110
BstUI	CGCG	2 cut(s)	207, 2000
BstV1I	GCAGC	5 cut(s)	138, 416, 1796, 1944, 2031
BstV2I	GAAGAC	1 cut(s)	216
BstX2I	RGATCY	1 cut(s)	325
BstYI	RGATCY	1 cut(s)	325
BstZ17I	GTATAC	1 cut(s)	889
BstZI	CGGCCG	1 cut(s)	202
Bsu15I	ATCGAT	1 cut(s)	694
BsuI	GTATCC	1 cut(s)	1866
BsuRI	GGCC	6 cut(s)	144, 176, 204, 548, 665, 1925
BsuTUI	ATCGAT	1 cut(s)	694
BtgI	CCRYGG	3 cut(s)	1340, 1689, 1977
BtgZI	GCGATG	1 cut(s)	1086
BtrI	CACGTC	3 cut(s)	783, 1423, 1831
BtsCI	GGATG	5 cut(s)	106, 601, 942, 973, 1634
BtsI	GCAGTG	1 cut(s)	196
BtsIMutI	CAGTG	2 cut(s)	196, 1382
BveI	ACCTGC	1 cut(s)	233
Cac8I	GCNNGC	7 cut(s)	184, 319, 1198, 1202, 1378, 1644, 1814
CaiI	CAGNNNCTG	1 cut(s)	851
CciI	TCATGA	3 cut(s)	249, 772, 1173
CciNI	GCGGCCGC	1 cut(s)	202
CfoI	GCGC	5 cut(s)	897, 1206, 1334, 1382, 2000
Cfr10I	RCCGGY	1 cut(s)	1642
Cfr13I	GGNCC	4 cut(s)	174, 487, 546, 767
ClaI	ATCGAT	1 cut(s)	694
CseI	GACGC	2 cut(s)	1124, 1989
Csp6I	GTAC	8 cut(s)	452, 483, 954, 990, 1004, 1270, 1276, 1616
CviQI	GTAC	8 cut(s)	452, 483, 954, 990, 1004, 1270, 1276, 1616
DdeI	CTNAG	2 cut(s)	322, 1744
DraIII	CACNNNGTG	1 cut(s)	120
DrdI	GACNNNNNNGTC	1 cut(s)	1999
DriI	GACNNNNNGTC	1 cut(s)	520
DseDI	GACNNNNNNGTC	1 cut(s)	1999
EaeI	YGGCCR	2 cut(s)	202, 1923
EagI	CGGCCG	1 cut(s)	202
Eam1104I	CTCTTC	4 cut(s)	812, 1151, 1312, 1499
Eam1105I	GACNNNNNGTC	1 cut(s)	520
EarI	CTCTTC	4 cut(s)	812, 1151, 1312, 1499
EciI	GGCGGA	2 cut(s)	1808, 2031
Ecl136II	GAGCTC	3 cut(s)	220, 685, 1627
EclXI	CGGCCG	1 cut(s)	202
Eco130I	CCWWGG	4 cut(s)	806, 997, 1340, 1689
Eco24I	GRGCYC	3 cut(s)	222, 687, 1629
Eco31I	GGTCTC	1 cut(s)	1828
Eco47I	GGWCC	2 cut(s)	487, 767
Eco52I	CGGCCG	1 cut(s)	202
Eco53kI	GAGCTC	3 cut(s)	220, 685, 1627
Eco57I	CTGAAG	1 cut(s)	1886
Eco88I	CYCGRG	3 cut(s)	730, 936, 1556
EcoICRI	GAGCTC	3 cut(s)	220, 685, 1627
EcoRII	CCWGG	2 cut(s)	264, 1327
EcoT14I	CCWWGG	4 cut(s)	806, 997, 1340, 1689
EcoT22I	ATGCAT	1 cut(s)	1402
EcoT38I	GRGCYC	3 cut(s)	222, 687, 1629
ErhI	CCWWGG	4 cut(s)	806, 997, 1340, 1689
FaqI	GGGAC	4 cut(s)	347, 380, 776, 1549
FauNDI	CATATG	1 cut(s)	913
FbaI	TGATCA	4 cut(s)	60, 103, 775, 1779
FblI	GTMKAC	5 cut(s)	510, 888, 1685, 1734, 2003
FokI	GGATG	5 cut(s)	113, 608, 929, 980, 1621
FriOI	GRGCYC	3 cut(s)	222, 687, 1629
FspBI	CTAG	2 cut(s)	1799, 1995
GlaI	GCGC	5 cut(s)	896, 1205, 1333, 1381, 1999
GsaI	CCCAGC	1 cut(s)	401
GsuI	CTGGAG	2 cut(s)	629, 1747
HaeII	RGCGCY	1 cut(s)	898
HaeIII	GGCC	6 cut(s)	144, 176, 204, 548, 665, 1925
HapII	CCGG	2 cut(s)	1541, 1643
HgaI	GACGC	2 cut(s)	1124, 1989
HhaI	GCGC	5 cut(s)	897, 1206, 1334, 1382, 2000
Hin6I	GCGC	5 cut(s)	895, 1204, 1332, 1380, 1998
HinP1I	GCGC	5 cut(s)	895, 1204, 1332, 1380, 1998
HincII	GTYRAC	3 cut(s)	361, 511, 1651
HindII	GTYRAC	3 cut(s)	361, 511, 1651
HinfI	GANTC	9 cut(s)	446, 515, 529, 740, 1052, 1537, 1754, 1760, 1973
HpaII	CCGG	2 cut(s)	1541, 1643
HphI	GGTGA	3 cut(s)	98, 214, 1959
Hpy188I	TCNGA	9 cut(s)	95, 293, 325, 408, 537, 576, 780, 1405, 1759
Hpy188III	TCNNGA	9 cut(s)	250, 773, 1174, 1186, 1242, 1445, 1556, 1657, 1764
Hpy99I	CGWCG	3 cut(s)	515, 625, 1886
HpyAV	CCTTC	6 cut(s)	46, 178, 817, 976, 1848, 1974
HpyCH4III	ACNGT	8 cut(s)	67, 297, 383, 625, 859, 1090, 1386, 1418
HpyCH4IV	ACGT	4 cut(s)	393, 782, 1422, 1830
HpyCH4V	TGCA	7 cut(s)	471, 843, 1108, 1127, 1236, 1400, 1816
HpyF10VI	GCNNNNNNNGC	4 cut(s)	671, 860, 1077, 1799
HpyF3I	CTNAG	2 cut(s)	322, 1744
HpySE526I	ACGT	4 cut(s)	393, 782, 1422, 1830
HspAI	GCGC	5 cut(s)	895, 1204, 1332, 1380, 1998
KroI	GCCGGC	1 cut(s)	1642
KroNI	GCCGGC	1 cut(s)	1644
Ksp22I	TGATCA	4 cut(s)	60, 103, 775, 1779
LguI	GCTCTTC	1 cut(s)	1312
LmnI	GCTCC	2 cut(s)	682, 1531
Lsp1109I	GCAGC	5 cut(s)	138, 416, 1796, 1944, 2031
LweI	GCATC	5 cut(s)	586, 661, 951, 1409, 1672
MaeI	CTAG	2 cut(s)	1799, 1995
MaeII	ACGT	4 cut(s)	393, 782, 1422, 1830
MaeIII	GTNAC	4 cut(s)	616, 931, 1418, 1507
MbiI	CCGCTC	1 cut(s)	1121
MfeI	CAATTG	1 cut(s)	1150
MflI	RGATCY	1 cut(s)	325
MhlI	GDGCHC	6 cut(s)	222, 633, 687, 788, 1110, 1629
MluCI	AATT	5 cut(s)	255, 541, 1042, 1150, 1678
MlyI	GAGTC	4 cut(s)	455, 509, 1531, 1754
Mph1103I	ATGCAT	1 cut(s)	1402
MroNI	GCCGGC	1 cut(s)	1642
MroXI	GAANNNNTTC	1 cut(s)	1305
MseI	TTAA	7 cut(s)	258, 701, 1464, 1707, 1857, 1890, 1943
MslI	CAYNNNNRTG	3 cut(s)	1101, 1339, 1787
MspA1I	CMGCKG	4 cut(s)	401, 607, 1957, 2022
MspI	CCGG	2 cut(s)	1541, 1643
MspR9I	CCNGG	3 cut(s)	266, 1329, 1542
MunI	CAATTG	1 cut(s)	1150
MvaI	CCWGG	2 cut(s)	266, 1329
MvnI	CGCG	2 cut(s)	207, 2000
MwoI	GCNNNNNNNGC	4 cut(s)	671, 860, 1077, 1799
NaeI	GCCGGC	1 cut(s)	1644
NciI	CCSGG	1 cut(s)	1542
NcoI	CCATGG	2 cut(s)	1340, 1689
NdeI	CATATG	1 cut(s)	913
NgoMIV	GCCGGC	1 cut(s)	1642
NlaIV	GGNNCC	2 cut(s)	410, 715
NmeAIII	GCCGAG	1 cut(s)	254
NmuCI	GTSAC	3 cut(s)	616, 1418, 1507
NotI	GCGGCCGC	1 cut(s)	202
NsiI	ATGCAT	1 cut(s)	1402
PaeR7I	CTCGAG	2 cut(s)	730, 936
PagI	TCATGA	3 cut(s)	249, 772, 1173
PaqCI	CACCTGC	1 cut(s)	233
PciSI	GCTCTTC	1 cut(s)	1312
PcsI	WCGNNNNNNNCGW	2 cut(s)	557, 1249
PdiI	GCCGGC	1 cut(s)	1644
PdmI	GAANNNNTTC	1 cut(s)	1305
PfeI	GAWTC	5 cut(s)	529, 740, 1052, 1754, 1973
PleI	GAGTC	4 cut(s)	454, 509, 1531, 1754
PpsI	GAGTC	4 cut(s)	454, 509, 1531, 1754
Psp124BI	GAGCTC	3 cut(s)	222, 687, 1629
Psp6I	CCWGG	2 cut(s)	264, 1327
PspFI	CCCAGC	1 cut(s)	397
PspGI	CCWGG	2 cut(s)	264, 1327
PspN4I	GGNNCC	2 cut(s)	410, 715
PspPI	GGNCC	4 cut(s)	174, 487, 546, 767
PspXI	VCTCGAGB	2 cut(s)	730, 936
PstI	CTGCAG	1 cut(s)	1818
PstNI	CAGNNNCTG	1 cut(s)	851
PsuI	RGATCY	1 cut(s)	325
PvuII	CAGCTG	1 cut(s)	401
RsaI	GTAC	8 cut(s)	453, 484, 955, 991, 1005, 1271, 1277, 1617
RsaNI	GTAC	8 cut(s)	452, 483, 954, 990, 1004, 1270, 1276, 1616
RseI	CAYNNNNRTG	3 cut(s)	1101, 1339, 1787
SacI	GAGCTC	3 cut(s)	222, 687, 1629
SalI	GTCGAC	1 cut(s)	509
SapI	GCTCTTC	1 cut(s)	1312
SaqAI	TTAA	7 cut(s)	258, 701, 1464, 1707, 1857, 1890, 1943
Sau96I	GGNCC	4 cut(s)	174, 487, 546, 767
ScaI	AGTACT	1 cut(s)	453
SchI	GAGTC	4 cut(s)	455, 509, 1531, 1754
ScrFI	CCNGG	3 cut(s)	266, 1329, 1542
SduI	GDGCHC	6 cut(s)	222, 633, 687, 788, 1110, 1629
SfaNI	GCATC	5 cut(s)	586, 661, 951, 1409, 1672
SfcI	CTRYAG	2 cut(s)	1600, 1814
Sfr274I	CTCGAG	2 cut(s)	730, 936
SinI	GGWCC	2 cut(s)	487, 767
SlaI	CTCGAG	2 cut(s)	730, 936
SmiMI	CAYNNNNRTG	3 cut(s)	1101, 1339, 1787
SmlI	CTYRAG	2 cut(s)	730, 936
SmoI	CTYRAG	2 cut(s)	730, 936
Sse9I	AATT	5 cut(s)	255, 541, 1042, 1150, 1678
SspI	AATATT	3 cut(s)	72, 565, 1438
SspMI	CTAG	2 cut(s)	1799, 1995
SstI	GAGCTC	3 cut(s)	222, 687, 1629
StyD4I	CCNGG	3 cut(s)	264, 1327, 1540
StyI	CCWWGG	4 cut(s)	806, 997, 1340, 1689
TaaI	ACNGT	8 cut(s)	67, 297, 383, 625, 859, 1090, 1386, 1418
TaiI	ACGT	4 cut(s)	396, 785, 1425, 1833
TasI	AATT	5 cut(s)	255, 541, 1042, 1150, 1678
TatI	WGTACW	3 cut(s)	451, 1003, 1275
TauI	GCSGC	7 cut(s)	89, 126, 144, 189, 204, 207, 607
TfiI	GAWTC	5 cut(s)	529, 740, 1052, 1754, 1973
Tru1I	TTAA	7 cut(s)	258, 701, 1464, 1707, 1857, 1890, 1943
Tru9I	TTAA	7 cut(s)	258, 701, 1464, 1707, 1857, 1890, 1943
TscAI	CASTG	2 cut(s)	203, 1389
TseFI	GTSAC	3 cut(s)	616, 1418, 1507
TseI	GCWGC	5 cut(s)	126, 429, 1809, 1957, 2019
Tsp45I	GTSAC	3 cut(s)	616, 1418, 1507
TspDTI	ATGAA	8 cut(s)	266, 1158, 1162, 1190, 1194, 1385, 1517, 1875
TspGWI	ACGGA	5 cut(s)	500, 780, 1302, 1326, 1966
TspRI	CASTG	2 cut(s)	203, 1389
VneI	GTGCAC	1 cut(s)	1106
VpaK11BI	GGWCC	2 cut(s)	487, 767
XhoI	CTCGAG	2 cut(s)	730, 936
XmiI	GTMKAC	5 cut(s)	510, 888, 1685, 1734, 2003
XmnI	GAANNNNTTC	1 cut(s)	1305
XspI	CTAG	2 cut(s)	1799, 1995
ZrmI	AGTACT	1 cut(s)	453
Zsp2I	ATGCAT	1 cut(s)	1402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.