Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000786

Physical Location & Seq

Genomic Coordinates Reverse (-)

24779453 .. 24784115

Gene Structure 4663 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G021370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGTCATCACGTATCTCTCACCTCCTTGCAGCTCTTCTTTCACTCTTGTTATTCAACTTCCATGGAAGCAAAGCCGACTTTATACCCACAAAAAATTGCCCCATATACAATTGTGGTAATGGCACTGATTTTCACTACCCTTTTTGGAAATTTGGAGACTCCACCGCCGGTCAATATTGTGGTTACCCTGGTTTTGGTCTCAATTGCTCAGAGTCCGGAGAGCCAATACTAAGTCTCCCAAATAATGGCTCCTACTATGTGAAAGCCATAAACTTTATTGATTCCACCATGACCCTTGTTGACCTCGACATCTTCGATCAACAATGCCCTAGGGCAAGACACAGTGTTAGTGTAGGCACGCTCCCATTGGATTATTCCCCACTGGATGTGAACCTGAGCTTCTACTTCAACTGCACTTCCTTTCCTGATCCTGTTGTGCCTCCCATAATCTGCTTAGGAATATGGGGTACCCAAATGTCTTATGTTTTCACGGAGGGAGAGCAATCAGATGGGTTTGATTGGTCTCAAAGTTGTGAGGAGAATGTGGTGGTGACTGTGATGAAGAGTAAGGAGCTTTATATTGATAAGTTGATTGGTGCTTTTGGAGGACTTATGAACCAGGGGTTTATGCTTAACTGGACCACGGCGAAGGAGTGCGCTTCGTGTGAGAGTGGTGGTGGTTTGTGTGGGTACAACCCCACTAAAGCTGATGAGTTTCTCTGCTTCTGCAAATATGGCAATGGCCTATGCAGGAAGAAAGGCCCCAAGTTCAACTGGATATCTAAGGATGTCGTAGGTCTTTGCTCGGTTATTGGAACTATAGCTGTAATGTGCTTCATTTTCTTTATACGGCGACGCCGCAACAGAAATCGATATGGTCCATCATCCTATGCATCTCGAAGCATCTATTCTAAAAGCTATTCTAGAACTGATACTAAGAAGGGAAGTGCATACCCTGGAGTTCATGTCTTCACTTACAAGGAACTTGAACAAGCAACAAACTATTTCGATTCATCTAAAAAACTTGGTGATGGAGGCTTTGGCACTGTTTACTATGGAAGAATCAGTGATGGGCGAGCAGTTGCTGTCAAGCGACTACATGAAACCAATTGCAAGAGAGTTGAGCAGTTCCTGAATGAAATTGAGATTTTAGCTCGCCTACGCCACCAAAACCTTGTCTTACTCTATGGATGCACCTCTCGTCACAGCCGTGAACTCCTCCTTGTCTACGAATATGTTCCCAATGGAACTCTTGCTAATCATCTTCACGGTGGAAAAGCAAAACCCGGTGCACTGCCATGGCATACTCGAATCAACATTGCCGTAGAAACTGCAAGTGCATTGTTCTATCTTCATGCGTCTGAGATCATCCACCGCGATGTGAAAACCACTAACATTCTCCTCGACAACAATTTCTGTGTCAAAGTAGCAGATTTTGGACTATCTCGTCTCTTTCCCATGGATGTCACCCATGTATCAACTGCTCCACAAGGAACTCCAGGTTATGTCGATCCTGATTATAACCAATGCTACCAGCTTACTACAAAGAGTGATGTCTATAGCTTCGGTGTGGTCCTGGTTGAACTCATATCATCCTTGCCTGCTGTTGATGTCACAAGGCATCGACAGGAGATCAATTTGTCGAACATGGCTATCAACAAGATTCAGAAAAATGCATTGCATGAGCTAGTTGATCCATCCCTGGGGTATGAATCAGACTTTAAGGTAAGAAAAATGATCAATGCAGTGGCGGAATTAGCGTTTCGGTGCCTGCAAAGTGAGACGGACATGCGACCTTCTATGGTTGATGTGGAAAATGAACTAAAGAGGATACAGAGTGATGATTTTACCGATGATGTTGTGCTGCTAAAGAGTGGTCCTCTGCCACCTTCACCAGATTCTGTGACATTAAATTGGGTTAGCATGTCCCCAACACCAAATGATAGTACTGGACTACTGATTAAACTTTTCCTTCTAGATTCATCTACGGTACCATTGTCATTCTCCTTTCATCTATATATTAAACATGTGATTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0008150 GO:0008152 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010228 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0031668 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0033993 GO:0036211 GO:0042221 GO:0042631 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071462 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0104004 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

679

Amino Acids

75.8

Weight (kDa)

6.8

Isoelectric Point (pI)

38.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	33 - 101	3.5e-16	Wall-associated receptor kinase galacturonan-binding
WAK_assoc	PF14380	158 - 244	1.7e-17	Wall-associated receptor kinase C-terminal
Pkinase	PF00069	337 - 604	2.2e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	339 - 608	4.6e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000786)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18390 AT1G18390
fragaria_vesca	FvH4_5g11760 FvH4_5g11760 FvH4_5g11790
malus_domestica	MD06G1218900.v1.1 MD06G1219200.v1.1 MD14G1228700.v1.1
prunus_persica	Prupe.5G223900_v2.0.a1 Prupe.5G223900_v2.0.a1 Prupe.5G223900_v2.0.a1
pyrus_communis	pycom06g19560 pycom06g19580 pycom14g19000
rosa_chinensis	RchiOBHm_Chr7g0185891 RchiOBHm_Chr7g0185941 RchiOBHm_Chr7g0185971 RchiOBHm_Chr7g0186001 RchiOBHm_Chr7g0186021 RchiOBHm_Chr7g0209991 RchiOBHm_Chr7g0210001
rosa_laevigata	RLG00000003071 RLG00000004872 RLG00000004873 RLG00000004875 RLG00000005413
rosa_multiflora	Rmu_co8007212.1_g000001 Rmu_co8076798.1_g000001 Rmu_sc0000194.1_g000013 Rmu_sc0000194.1_g000017 Rmu_sc0000194.1_g000019 Rmu_sc0004509.1_g000017 Rmu_sc0004509.1_g000018 Rmu_sc0005665.1_g000002 Rmu_sc0005665.1_g000004 Rmu_sc0005665.1_g000020 Rmu_sc0005665.1_g000023 Rmu_sc0005665.1_g000035
rosa_roxburghii	Rroxscaffold_1G00039510 Rroxscaffold_3G00248670 Rroxscaffold_3G00257880 Rroxscaffold_3G00268500 Rroxscaffold_3G00268510 Rroxscaffold_3G00268530 Rroxscaffold_3G00268580
rosa_rugosa	Rorug06G0471800 Rorug07G0117900
rosa_samantha	Rh1BG104700 Rh4CG290300 Rh7AG070300 Rh7AG070400 Rh7AG070600 Rh7AG251700 Rh7BG086300 Rh7BG086400 Rh7BG246300 Rh7CG077400 Rh7CG077800 Rh7CG078000 Rh7CG268500 Rh7DG077400 Rh7DG077600 Rh7DG077900 Rh7DG078000 Rh7DG258500
rosa_wichuraiana	Rw7G006440 Rw7G006470 Rw7G006480 Rw7G021370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1521
AasI	GACNNNNNNGTC	1 cut(s)	1446
Acc65I	GGTACC	2 cut(s)	467, 1990
AccB1I	GGYRCC	3 cut(s)	467, 1767, 1990
AccB7I	CCANNNNNTGG	1 cut(s)	245
AccI	GTMKAC	1 cut(s)	1227
AccII	CGCG	1 cut(s)	1377
AccIII	TCCGGA	1 cut(s)	215
AciI	CCGC	4 cut(s)	165, 859, 1375, 1751
AclWI	GGATC	3 cut(s)	422, 1505, 1688
AcsI	RAATTY	1 cut(s)	149
AcyI	GRCGYC	1 cut(s)	856
AfaI	GTAC	4 cut(s)	469, 692, 1948, 1992
AfiI	CCNNNNNNNGG	3 cut(s)	194, 245, 1703
AflIII	ACRYGT	1 cut(s)	2026
AgsI	TTSAA	5 cut(s)	55, 409, 772, 989, 1583
AjnI	CCWGG	6 cut(s)	187, 618, 955, 1498, 1575, 1701
AleI	CACNNNNGTG	1 cut(s)	1209
Alw21I	GWGCWC	1 cut(s)	1294
Alw26I	GTCTC	6 cut(s)	150, 203, 239, 528, 1454, 1775
Alw44I	GTGCAC	1 cut(s)	1290
AlwI	GGATC	3 cut(s)	422, 1505, 1688
AlwNI	CAGNNNCTG	3 cut(s)	1085, 1132, 1901
Aor13HI	TCCGGA	1 cut(s)	215
AoxI	GGCC	2 cut(s)	742, 760
ApaLI	GTGCAC	1 cut(s)	1290
ApeKI	GCWGC	2 cut(s)	29, 1864
ApoI	RAATTY	1 cut(s)	149
Asp700I	GAANNNNTTC	1 cut(s)	1236
Asp718I	GGTACC	2 cut(s)	467, 1990
AspA2I	CCTAGG	1 cut(s)	329
AspLEI	GCGC	1 cut(s)	659
AspS9I	GGNCC	5 cut(s)	639, 761, 878, 1573, 1877
AsuC2I	CCSGG	1 cut(s)	1287
AsuHPI	GGTGA	5 cut(s)	11, 562, 1040, 1459, 1884
AvaII	GGWCC	4 cut(s)	639, 878, 1573, 1877
AvrII	CCTAGG	1 cut(s)	329
BaeGI	GKGCMC	1 cut(s)	1294
BaeI	ACNNNNGTAYC	4 cut(s)	1458, 1491, 1982, 2015
BanI	GGYRCC	3 cut(s)	467, 1767, 1990
BbsI	GAAGAC	1 cut(s)	961
Bbv12I	GWGCWC	1 cut(s)	1294
BbvI	GCAGC	2 cut(s)	41, 1851
BccI	CCATC	5 cut(s)	503, 889, 1025, 1064, 1705
BceAI	ACGGC	4 cut(s)	660, 866, 1194, 1307
BciT130I	CCWGG	6 cut(s)	189, 620, 957, 1500, 1577, 1703
BciVI	GTATCC	1 cut(s)	1824
BclI	TGATCA	1 cut(s)	1737
BcnI	CCSGG	1 cut(s)	1287
BcoDI	GTCTC	6 cut(s)	150, 203, 239, 528, 1454, 1775
BfaI	CTAG	4 cut(s)	330, 924, 1688, 1976
BfmI	CTRYAG	2 cut(s)	819, 1558
BfuI	GTATCC	1 cut(s)	1824
BisI	GCNGC	3 cut(s)	30, 859, 1865
BlnI	CCTAGG	1 cut(s)	329
BlsI	GCNGC	3 cut(s)	31, 860, 1866
BmcAI	AGTACT	1 cut(s)	1948
Bme1390I	CCNGG	7 cut(s)	189, 620, 957, 1287, 1500, 1577, 1703
Bme18I	GGWCC	4 cut(s)	639, 878, 1573, 1877
BmgT120I	GGNCC	5 cut(s)	639, 761, 878, 1573, 1877
BmiI	GGNNCC	5 cut(s)	250, 469, 763, 1769, 1992
BmrFI	CCNGG	7 cut(s)	189, 620, 957, 1287, 1500, 1577, 1703
BmsI	GCATC	4 cut(s)	902, 912, 1181, 1630
BpiI	GAAGAC	1 cut(s)	961
BplI	GAGNNNNNCTC	2 cut(s)	1864, 1896
BpmI	CTGGAG	2 cut(s)	978, 1482
Bpu10I	CCTNAGC	2 cut(s)	395, 454
BpuMI	CCSGG	1 cut(s)	1287
Bsa29I	ATCGAT	1 cut(s)	871
BsaAI	YACGTR	1 cut(s)	11
BsaHI	GRCGYC	1 cut(s)	856
BsaI	GGTCTC	2 cut(s)	203, 528
BsaWI	WCCGGW	1 cut(s)	215
BsaXI	ACNNNNNCTCC	4 cut(s)	219, 249, 951, 981
Bsc4I	CCNNNNNNNGG	3 cut(s)	194, 245, 1703
Bse118I	RCCGGY	1 cut(s)	167
Bse1I	ACTGG	4 cut(s)	387, 641, 779, 1954
Bse3DI	GCAATG	3 cut(s)	745, 1317, 1676
BseAI	TCCGGA	1 cut(s)	215
BseBI	CCWGG	6 cut(s)	189, 620, 957, 1500, 1577, 1703
BseCI	ATCGAT	1 cut(s)	871
BseGI	GGATG	8 cut(s)	391, 793, 884, 1196, 1368, 1468, 1592, 1697
BseLI	CCNNNNNNNGG	3 cut(s)	194, 245, 1703
BseMI	GCAATG	3 cut(s)	745, 1317, 1676
BseMII	CTCAG	3 cut(s)	222, 386, 1353
BseNI	ACTGG	4 cut(s)	387, 641, 779, 1954
BseRI	GAGGAG	3 cut(s)	551, 1208, 1391
BseSI	GKGCMC	1 cut(s)	1294
BseXI	GCAGC	2 cut(s)	41, 1851
BsgI	GTGCAG	1 cut(s)	397
Bsh1236I	CGCG	1 cut(s)	1377
BshFI	GGCC	2 cut(s)	744, 762
BshNI	GGYRCC	3 cut(s)	467, 1767, 1990
BshVI	ATCGAT	1 cut(s)	871
BsiHKAI	GWGCWC	1 cut(s)	1294
BsiSI	CCGG	3 cut(s)	168, 216, 1287
BslFI	GGGAC	1 cut(s)	1912
BslI	CCNNNNNNNGG	3 cut(s)	194, 245, 1703
BsmAI	GTCTC	6 cut(s)	150, 203, 239, 528, 1454, 1775
BsmBI	CGTCTC	2 cut(s)	1454, 1775
BsmFI	GGGAC	1 cut(s)	1912
BsnI	GGCC	2 cut(s)	744, 762
Bso31I	GGTCTC	2 cut(s)	203, 528
Bsp1286I	GDGCHC	1 cut(s)	1294
Bsp13I	TCCGGA	1 cut(s)	215
Bsp143I	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
Bsp19I	CCATGG	3 cut(s)	61, 1298, 1458
BspACI	CCGC	4 cut(s)	165, 859, 1375, 1751
BspANI	GGCC	2 cut(s)	744, 762
BspCNI	CTCAG	3 cut(s)	221, 387, 1354
BspDI	ATCGAT	1 cut(s)	871
BspEI	TCCGGA	1 cut(s)	215
BspFNI	CGCG	1 cut(s)	1377
BspLI	GGNNCC	5 cut(s)	250, 469, 763, 1769, 1992
BspPI	GGATC	3 cut(s)	422, 1505, 1688
BspQI	GCTCTTC	1 cut(s)	39
BspT107I	GGYRCC	3 cut(s)	467, 1767, 1990
BspTNI	GGTCTC	2 cut(s)	203, 528
BsrDI	GCAATG	3 cut(s)	745, 1317, 1676
BsrFI	RCCGGY	1 cut(s)	167
BsrI	ACTGG	4 cut(s)	387, 641, 779, 1954
BssAI	RCCGGY	1 cut(s)	167
BssMI	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
BssNI	GRCGYC	1 cut(s)	856
BssT1I	CCWWGG	4 cut(s)	61, 329, 1298, 1458
Bst2UI	CCWGG	6 cut(s)	189, 620, 957, 1500, 1577, 1703
Bst4CI	ACNGT	5 cut(s)	344, 556, 1048, 1271, 1990
Bst6I	CTCTTC	2 cut(s)	39, 557
BstACI	GRCGYC	1 cut(s)	856
BstBAI	YACGTR	1 cut(s)	11
BstC8I	GCNNGC	5 cut(s)	359, 1077, 1156, 1602, 1772
BstDEI	CTNAG	7 cut(s)	208, 230, 395, 454, 783, 936, 1362
BstDSI	CCRYGG	4 cut(s)	61, 642, 1298, 1458
BstEII	GGTNACC	1 cut(s)	182
BstF5I	GGATG	8 cut(s)	391, 793, 884, 1196, 1368, 1468, 1592, 1697
BstFNI	CGCG	1 cut(s)	1377
BstHHI	GCGC	1 cut(s)	659
BstKTI	GATC	7 cut(s)	319, 430, 1368, 1513, 1635, 1696, 1740
BstMAI	GTCTC	6 cut(s)	150, 203, 239, 528, 1454, 1775
BstMBI	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
BstMWI	GCNNNNNNNGC	2 cut(s)	735, 1757
BstNI	CCWGG	6 cut(s)	189, 620, 957, 1500, 1577, 1703
BstNSI	RCATGY	3 cut(s)	1792, 1927, 2030
BstPI	GGTNACC	1 cut(s)	182
BstSCI	CCNGG	7 cut(s)	187, 618, 955, 1285, 1498, 1575, 1701
BstSFI	CTRYAG	2 cut(s)	819, 1558
BstSLI	GKGCMC	1 cut(s)	1294
BstUI	CGCG	1 cut(s)	1377
BstV1I	GCAGC	2 cut(s)	41, 1851
BstV2I	GAAGAC	1 cut(s)	961
Bsu15I	ATCGAT	1 cut(s)	871
BsuI	GTATCC	1 cut(s)	1824
BsuRI	GGCC	2 cut(s)	744, 762
BsuTUI	ATCGAT	1 cut(s)	871
BtgI	CCRYGG	4 cut(s)	61, 642, 1298, 1458
BtgZI	GCGATG	1 cut(s)	1392
BtsCI	GGATG	8 cut(s)	391, 793, 884, 1196, 1368, 1468, 1592, 1697
BtsI	GCAGTG	2 cut(s)	1292, 1752
BtsIMutI	CAGTG	7 cut(s)	123, 349, 380, 1044, 1072, 1292, 1752
Cac8I	GCNNGC	5 cut(s)	359, 1077, 1156, 1602, 1772
CaiI	CAGNNNCTG	3 cut(s)	1085, 1132, 1901
CfoI	GCGC	1 cut(s)	659
Cfr10I	RCCGGY	1 cut(s)	167
Cfr13I	GGNCC	5 cut(s)	639, 761, 878, 1573, 1877
ClaI	ATCGAT	1 cut(s)	871
CseI	GACGC	2 cut(s)	864, 1347
Csp6I	GTAC	4 cut(s)	468, 691, 1947, 1991
CviQI	GTAC	4 cut(s)	468, 691, 1947, 1991
DdeI	CTNAG	7 cut(s)	208, 230, 395, 454, 783, 936, 1362
DpnI	GATC	7 cut(s)	318, 429, 1367, 1512, 1634, 1695, 1739
DpnII	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
DrdI	GACNNNNNNGTC	1 cut(s)	1446
DseDI	GACNNNNNNGTC	1 cut(s)	1446
Eam1104I	CTCTTC	2 cut(s)	39, 557
EarI	CTCTTC	2 cut(s)	39, 557
EciI	GGCGGA	1 cut(s)	1766
Eco130I	CCWWGG	4 cut(s)	61, 329, 1298, 1458
Eco31I	GGTCTC	2 cut(s)	203, 528
Eco32I	GATATC	1 cut(s)	780
Eco47I	GGWCC	4 cut(s)	639, 878, 1573, 1877
Eco91I	GGTNACC	1 cut(s)	182
EcoO109I	RGGNCCY	1 cut(s)	761
EcoO65I	GGTNACC	1 cut(s)	182
EcoRII	CCWGG	6 cut(s)	187, 618, 955, 1498, 1575, 1701
EcoRV	GATATC	1 cut(s)	780
EcoT14I	CCWWGG	4 cut(s)	61, 329, 1298, 1458
EcoT22I	ATGCAT	2 cut(s)	895, 1678
ErhI	CCWWGG	4 cut(s)	61, 329, 1298, 1458
Esp3I	CGTCTC	2 cut(s)	1454, 1775
FaqI	GGGAC	1 cut(s)	1912
FbaI	TGATCA	1 cut(s)	1737
FblI	GTMKAC	1 cut(s)	1227
Fnu4HI	GCNGC	3 cut(s)	30, 859, 1865
FokI	GGATG	8 cut(s)	398, 800, 871, 1203, 1355, 1475, 1579, 1684
Fsp4HI	GCNGC	3 cut(s)	30, 859, 1865
FspBI	CTAG	4 cut(s)	330, 924, 1688, 1976
GlaI	GCGC	1 cut(s)	658
GluI	GCNGC	3 cut(s)	30, 859, 1865
GsuI	CTGGAG	2 cut(s)	978, 1482
HaeIII	GGCC	2 cut(s)	744, 762
HapII	CCGG	3 cut(s)	168, 216, 1287
HgaI	GACGC	2 cut(s)	864, 1347
HhaI	GCGC	1 cut(s)	659
Hin1I	GRCGYC	1 cut(s)	856
Hin6I	GCGC	1 cut(s)	657
HinP1I	GCGC	1 cut(s)	657
HincII	GTYRAC	1 cut(s)	301
HindII	GTYRAC	1 cut(s)	301
HpaII	CCGG	3 cut(s)	168, 216, 1287
HphI	GGTGA	5 cut(s)	11, 562, 1040, 1459, 1884
Hpy166II	GTNNAC	6 cut(s)	301, 391, 1051, 1214, 1228, 1292
Hpy188I	TCNGA	5 cut(s)	211, 508, 1363, 1668, 1717
Hpy188III	TCNNGA	7 cut(s)	216, 425, 897, 924, 1132, 1514, 1976
Hpy8I	GTNNAC	6 cut(s)	301, 391, 1051, 1214, 1228, 1292
Hpy99I	CGWCG	1 cut(s)	858
HpyAV	CCTTC	5 cut(s)	643, 934, 1806, 1899, 1982
HpyCH4III	ACNGT	5 cut(s)	344, 556, 1048, 1271, 1990
HpyCH4IV	ACGT	1 cut(s)	10
HpyF10VI	GCNNNNNNNGC	2 cut(s)	735, 1757
HpyF3I	CTNAG	7 cut(s)	208, 230, 395, 454, 783, 936, 1362
HpySE526I	ACGT	1 cut(s)	10
Hsp92I	GRCGYC	1 cut(s)	856
HspAI	GCGC	1 cut(s)	657
Kpn2I	TCCGGA	1 cut(s)	215
KpnI	GGTACC	2 cut(s)	471, 1994
Ksp22I	TGATCA	1 cut(s)	1737
Kzo9I	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
LguI	GCTCTTC	1 cut(s)	39
LmnI	GCTCC	4 cut(s)	254, 366, 571, 1489
Lsp1109I	GCAGC	2 cut(s)	41, 1851
LweI	GCATC	4 cut(s)	902, 912, 1181, 1630
MaeI	CTAG	4 cut(s)	330, 924, 1688, 1976
MaeII	ACGT	1 cut(s)	10
MaeIII	GTNAC	6 cut(s)	182, 550, 1202, 1465, 1612, 1903
MalI	GATC	7 cut(s)	318, 429, 1367, 1512, 1634, 1695, 1739
MboI	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
MboII	GAAGA	8 cut(s)	26, 304, 574, 766, 961, 1071, 1256, 1343
MfeI	CAATTG	3 cut(s)	109, 202, 1108
MhlI	GDGCHC	1 cut(s)	1294
MlyI	GAGTC	2 cut(s)	152, 221
Mph1103I	ATGCAT	2 cut(s)	895, 1678
MroI	TCCGGA	1 cut(s)	215
MroXI	GAANNNNTTC	1 cut(s)	1236
MseI	TTAA	5 cut(s)	633, 1722, 1910, 1962, 2022
MslI	CAYNNNNRTG	3 cut(s)	1209, 1297, 1377
MspI	CCGG	3 cut(s)	168, 216, 1287
MspR9I	CCNGG	7 cut(s)	189, 620, 957, 1287, 1500, 1577, 1703
MunI	CAATTG	3 cut(s)	109, 202, 1108
MvaI	CCWGG	6 cut(s)	189, 620, 957, 1500, 1577, 1703
MvnI	CGCG	1 cut(s)	1377
MwoI	GCNNNNNNNGC	2 cut(s)	735, 1757
NciI	CCSGG	1 cut(s)	1287
NcoI	CCATGG	3 cut(s)	61, 1298, 1458
NdeII	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
NlaIV	GGNNCC	5 cut(s)	250, 469, 763, 1769, 1992
NmuCI	GTSAC	5 cut(s)	550, 1202, 1465, 1612, 1903
NsiI	ATGCAT	2 cut(s)	895, 1678
NspI	RCATGY	3 cut(s)	1792, 1927, 2030
OliI	CACNNNNGTG	1 cut(s)	1209
PasI	CCCWGGG	1 cut(s)	1702
PciI	ACATGT	1 cut(s)	2026
PciSI	GCTCTTC	1 cut(s)	39
PcsI	WCGNNNNNNNCGW	2 cut(s)	312, 1207
PdmI	GAANNNNTTC	1 cut(s)	1236
PfeI	GAWTC	8 cut(s)	281, 1010, 1062, 1311, 1663, 1712, 1898, 1979
PflMI	CCANNNNNTGG	1 cut(s)	245
PkrI	GCNGC	3 cut(s)	31, 860, 1866
PleI	GAGTC	2 cut(s)	152, 220
PpsI	GAGTC	2 cut(s)	152, 220
Ppu21I	YACGTR	1 cut(s)	11
PscI	ACATGT	1 cut(s)	2026
PsiI	TTATAA	1 cut(s)	1521
Psp6I	CCWGG	6 cut(s)	187, 618, 955, 1498, 1575, 1701
PspEI	GGTNACC	1 cut(s)	182
PspGI	CCWGG	6 cut(s)	187, 618, 955, 1498, 1575, 1701
PspN4I	GGNNCC	5 cut(s)	250, 469, 763, 1769, 1992
PspPI	GGNCC	5 cut(s)	639, 761, 878, 1573, 1877
PstNI	CAGNNNCTG	3 cut(s)	1085, 1132, 1901
RsaI	GTAC	4 cut(s)	469, 692, 1948, 1992
RsaNI	GTAC	4 cut(s)	468, 691, 1947, 1991
RseI	CAYNNNNRTG	3 cut(s)	1209, 1297, 1377
SapI	GCTCTTC	1 cut(s)	39
SaqAI	TTAA	5 cut(s)	633, 1722, 1910, 1962, 2022
SatI	GCNGC	3 cut(s)	30, 859, 1865
Sau3AI	GATC	7 cut(s)	316, 427, 1365, 1510, 1632, 1693, 1737
Sau96I	GGNCC	5 cut(s)	639, 761, 878, 1573, 1877
ScaI	AGTACT	1 cut(s)	1948
SchI	GAGTC	2 cut(s)	152, 221
ScrFI	CCNGG	7 cut(s)	189, 620, 957, 1287, 1500, 1577, 1703
SduI	GDGCHC	1 cut(s)	1294
SfaNI	GCATC	4 cut(s)	902, 912, 1181, 1630
SfcI	CTRYAG	2 cut(s)	819, 1558
SinI	GGWCC	4 cut(s)	639, 878, 1573, 1877
SmiMI	CAYNNNNRTG	3 cut(s)	1209, 1297, 1377
SsiI	CCGC	4 cut(s)	165, 859, 1375, 1751
SspI	AATATT	1 cut(s)	176
SspMI	CTAG	4 cut(s)	330, 924, 1688, 1976
StyD4I	CCNGG	7 cut(s)	187, 618, 955, 1285, 1498, 1575, 1701
StyI	CCWWGG	4 cut(s)	61, 329, 1298, 1458
TaaI	ACNGT	5 cut(s)	344, 556, 1048, 1271, 1990
TaiI	ACGT	1 cut(s)	13
TatI	WGTACW	1 cut(s)	1946
TauI	GCSGC	1 cut(s)	861
TfiI	GAWTC	8 cut(s)	281, 1010, 1062, 1311, 1663, 1712, 1898, 1979
Tru1I	TTAA	5 cut(s)	633, 1722, 1910, 1962, 2022
Tru9I	TTAA	5 cut(s)	633, 1722, 1910, 1962, 2022
TscAI	CASTG	7 cut(s)	130, 349, 387, 1051, 1072, 1299, 1752
TseFI	GTSAC	5 cut(s)	550, 1202, 1465, 1612, 1903
TseI	GCWGC	2 cut(s)	29, 1864
Tsp45I	GTSAC	5 cut(s)	550, 1202, 1465, 1612, 1903
TspGWI	ACGGA	2 cut(s)	506, 1799
TspRI	CASTG	7 cut(s)	130, 349, 387, 1051, 1072, 1299, 1752
Van91I	CCANNNNNTGG	1 cut(s)	245
VneI	GTGCAC	1 cut(s)	1290
VpaK11BI	GGWCC	4 cut(s)	639, 878, 1573, 1877
XapI	RAATTY	1 cut(s)	149
XbaI	TCTAGA	2 cut(s)	923, 1975
XceI	RCATGY	3 cut(s)	1792, 1927, 2030
XmaJI	CCTAGG	1 cut(s)	329
XmiI	GTMKAC	1 cut(s)	1227
XmnI	GAANNNNTTC	1 cut(s)	1236
XspI	CTAG	4 cut(s)	330, 924, 1688, 1976
ZrmI	AGTACT	1 cut(s)	1948
Zsp2I	ATGCAT	2 cut(s)	895, 1678

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.