Rmu_sc0005665.1_g000004

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005665.1
OG0000786
Physical Location & Seq
Forward (+)
12014 .. 12643
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005665.1_g000004.1.cds

Sequence Viewer

Length: 630 bp
atgtcatctcctatctcttatcttcttgcagctcttctctcactcttgttattcaactttcatggaagcaaagcccactttgaacccacaaaaaattgccccatatacaattgtggtaatggtcctgattttcactaccctttttggaaaattgaagactccaccgccgatcaatattgtggttaccctggttttggtctcaattgctcagagtctggagagcccatactgagtctcccaaataatgatttctactatgtgaaagacataaactttactgattccaccatcacccttgttaacattgacgtcgtcgatcaacaatgccctaggccaagacacagtatgagtgtaggcacgctcccattggattattccccattggatgtgaacctgagcttctacttcaactgcacttcctttcctggtccttttaagcctcccaaaacctgcttgggaatatggggtaccaaaaattcttacgttttcacagagggaaatgaaccagatgggtttgattggtctgaaagttgtgaggagaatgtggtggtgacggtgatgaagactgaggagctttataataatttgagtagtgattttggagggcttatgaagcagtgttggaggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

209

Amino Acids

23.54

Weight (kDa)

4.54

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 1 cut(s) 458
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AciI CCGC 1 cut(s) 165
AcsI RAATTY 1 cut(s) 475
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 4 cut(s) 55, 83, 155, 409
AjnI CCWGG 2 cut(s) 187, 424
AluBI AGCT 3 cut(s) 32, 399, 574
AluI AGCT 3 cut(s) 32, 399, 574
Alw26I GTCTC 2 cut(s) 203, 239
AlwNI CAGNNNCTG 1 cut(s) 215
AoxI GGCC 1 cut(s) 332
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 475
Asp718I GGTACC 1 cut(s) 467
AspA2I CCTAGG 1 cut(s) 329
AspS9I GGNCC 2 cut(s) 122, 428
AsuHPI GGTGA 3 cut(s) 283, 562, 568
AvaII GGWCC 2 cut(s) 122, 428
AvrII CCTAGG 1 cut(s) 329
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 2 cut(s) 162, 569
BbvI GCAGC 1 cut(s) 41
BccI CCATC 2 cut(s) 296, 503
BciT130I CCWGG 2 cut(s) 189, 426
BcoDI GTCTC 2 cut(s) 203, 239
BfaI CTAG 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 458
BisI GCNGC 1 cut(s) 30
BlnI CCTAGG 1 cut(s) 329
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 2 cut(s) 189, 426
Bme18I GGWCC 2 cut(s) 122, 428
BmgT120I GGNCC 2 cut(s) 122, 428
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 189, 426
BpiI GAAGAC 2 cut(s) 162, 569
BpmI CTGGAG 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 2 cut(s) 187, 329
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 1 cut(s) 194
BseBI CCWGG 2 cut(s) 189, 426
BseDI CCNNGG 2 cut(s) 187, 329
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 4 cut(s) 221, 222, 386, 558
BseRI GAGGAG 2 cut(s) 551, 584
BseXI GCAGC 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 1 cut(s) 334
BshNI GGYRCC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 2 cut(s) 203, 239
BsnI GGCC 1 cut(s) 334
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 2 cut(s) 169, 316
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 4 cut(s) 221, 222, 387, 559
BspLI GGNNCC 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 458
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 187, 329
BssMI GATC 2 cut(s) 169, 316
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 329
Bst2UI CCWGG 2 cut(s) 189, 426
Bst4CI ACNGT 2 cut(s) 344, 556
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 4 cut(s) 208, 230, 395, 567
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 2 cut(s) 172, 319
BstMAI GTCTC 2 cut(s) 203, 239
BstMBI GATC 2 cut(s) 169, 316
BstMWI GCNNNNNNNGC 1 cut(s) 613
BstNI CCWGG 2 cut(s) 189, 426
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 187, 424
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 2 cut(s) 162, 569
BsuRI GGCC 1 cut(s) 334
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 1 cut(s) 623
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 1 cut(s) 359
CaiI CAGNNNCTG 1 cut(s) 215
Cfr13I GGNCC 2 cut(s) 122, 428
Csp6I GTAC 1 cut(s) 468
CviAII CATG 1 cut(s) 62
CviJI RGCY 8 cut(s) 32, 74, 223, 334, 399, 439, 574, 607
CviKI_1 RGCY 8 cut(s) 32, 74, 223, 334, 399, 439, 574, 607
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 4 cut(s) 208, 230, 395, 567
DpnI GATC 2 cut(s) 171, 318
DpnII GATC 2 cut(s) 169, 316
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 122, 428
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 187, 424
EcoT14I CCWWGG 1 cut(s) 329
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 1 cut(s) 65
FatI CATG 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 398
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 330
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 1 cut(s) 334
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 1 cut(s) 65
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 4 cut(s) 158, 212, 232, 281
HpaI GTTAAC 1 cut(s) 301
HphI GGTGA 3 cut(s) 283, 562, 568
Hpy166II GTNNAC 2 cut(s) 301, 391
Hpy188I TCNGA 2 cut(s) 211, 526
Hpy188III TCNNGA 2 cut(s) 125, 216
Hpy8I GTNNAC 2 cut(s) 301, 391
Hpy99I CGWCG 2 cut(s) 314, 317
HpyCH4III ACNGT 2 cut(s) 344, 556
HpyCH4IV ACGT 2 cut(s) 309, 483
HpyCH4V TGCA 2 cut(s) 29, 414
HpyF10VI GCNNNNNNNGC 1 cut(s) 613
HpyF3I CTNAG 4 cut(s) 208, 230, 395, 567
HpySE526I ACGT 2 cut(s) 309, 483
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 1 cut(s) 65
KpnI GGTACC 1 cut(s) 471
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 169, 316
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 366, 571
LpnPI CCDG 9 cut(s) 138, 174, 201, 201, 407, 411, 438, 463, 519
Lsp1109I GCAGC 1 cut(s) 41
MaeI CTAG 1 cut(s) 330
MaeII ACGT 2 cut(s) 309, 483
MaeIII GTNAC 2 cut(s) 182, 550
MalI GATC 2 cut(s) 171, 318
MboI GATC 2 cut(s) 169, 316
MboII GAAGA 4 cut(s) 14, 26, 167, 574
MfeI CAATTG 2 cut(s) 109, 202
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 6 cut(s) 94, 109, 150, 202, 475, 583
MlyI GAGTC 3 cut(s) 152, 221, 241
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 6 cut(s) 450, 487, 529, 562, 596, 618
MseI TTAA 2 cut(s) 300, 435
MspR9I CCNGG 2 cut(s) 189, 426
MunI CAATTG 2 cut(s) 109, 202
MvaI CCWGG 2 cut(s) 189, 426
MwoI GCNNNNNNNGC 1 cut(s) 613
NdeII GATC 2 cut(s) 169, 316
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 550
PciSI GCTCTTC 1 cut(s) 39
PfeI GAWTC 1 cut(s) 281
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 3 cut(s) 152, 220, 240
PpsI GAGTC 3 cut(s) 152, 220, 240
PsiI TTATAA 1 cut(s) 579
Psp6I CCWGG 2 cut(s) 187, 424
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 187, 424
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 2 cut(s) 122, 428
PstNI CAGNNNCTG 1 cut(s) 215
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 300, 435
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 169, 316
Sau96I GGNCC 2 cut(s) 122, 428
SchI GAGTC 3 cut(s) 152, 221, 241
ScrFI CCNGG 2 cut(s) 189, 426
SduI GDGCHC 1 cut(s) 225
SetI ASST 8 cut(s) 34, 312, 396, 401, 452, 486, 576, 629
SinI GGWCC 2 cut(s) 122, 428
Sse9I AATT 6 cut(s) 94, 109, 150, 202, 475, 583
SsiI CCGC 1 cut(s) 165
SspI AATATT 1 cut(s) 176
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 187, 424
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 2 cut(s) 344, 556
TaiI ACGT 2 cut(s) 312, 486
TaqI TCGA 1 cut(s) 315
TasI AATT 6 cut(s) 94, 109, 150, 202, 475, 583
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 2 cut(s) 300, 435
Tru9I TTAA 2 cut(s) 300, 435
TscAI CASTG 1 cut(s) 623
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 4 cut(s) 50, 516, 575, 626
TspRI CASTG 1 cut(s) 623
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 122, 428
XapI RAATTY 1 cut(s) 475
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmaJI CCTAGG 1 cut(s) 329
XspI CTAG 1 cut(s) 330
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.