RchiOBHm_Chr7g0185891

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000786
Physical Location & Seq
Forward (+)
5948612 .. 5950603
1992 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16593

Sequence Viewer

Length: 744 bp
ATGTACAGTTGTGGTAATGACGTTTATTTTCACTACCCTTTTTGGAAAATCGAAGACTCCACAGCCCATCAACATTGTGGATACCCTGGTTTTGGTCTAAGATGCTCGGATTCTGGAGAGCCCATGCTGGGTCTCCCAAACAATGTCTCCTACTATGTGAAAGACTTAAACTTTATTGCATCCACCATCACCCTTGTTGACGTTGATGTTGTTCATCAAGCATGCCCTAGGGCAAGACACAATATTAGTTTGGGCACGCTCCCATTGGATTACTCCCCACTGGATGTGAACCTGAGCTTCTACTTCAACTGCACTTTAGATGCTGATCATGTTGTGCCTCCTATAACCTGTTTGGGAATATATGGTATCAAAAAGTCTTACGTTTTCACGGAGGGAGAGGAACCAGATGGGTTTGATTGGTCTGAAAGTTGTGAGGAGAATGTGGTGGTGACTGTGAAAGAAACAGAGATTACTAGTAGTATTGATGAGTTGATTGGTTCATTTGGAGTGGCCATGAACAATGGGTTTGTGCTTAACTGGACTGTGGCTAAGGAGTGCGATTCCTGTGAGGTTAATGGTGGCCTTTGTGGCTACAAGAACCTTACTGTGCCAGAGTTTCTGTGCTTTTGCAGAGATGGTAGTATTGGTACCAGAAACAATGGCCTCTGCAAGAAATTAGGTATGTTTGTCGATGATGCTCTCTCTTTATCTAATTATAAAGCTATGGGTCTGTTTGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

247

Amino Acids

27.32

Weight (kDa)

4.51

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 3 - 67 1.3e-14 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 123 - 213 5e-19 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
Acc65I GGTACC 1 cut(s) 647
AccB1I GGYRCC 1 cut(s) 647
AcoI YGGCCR 1 cut(s) 510
AfaI GTAC 2 cut(s) 5, 649
AfiI CCNNNNNNNGG 2 cut(s) 92, 128
AgsI TTSAA 1 cut(s) 307
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 2 cut(s) 297, 722
AluI AGCT 2 cut(s) 297, 722
Alw26I GTCTC 2 cut(s) 137, 151
AoxI GGCC 3 cut(s) 510, 580, 661
Asp718I GGTACC 1 cut(s) 647
AspA2I CCTAGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 181, 460
AvrII CCTAGG 1 cut(s) 227
BaeGI GKGCMC 1 cut(s) 257
BaeI ACNNNNGTAYC 2 cut(s) 73, 106
BalI TGGCCA 1 cut(s) 512
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 60
BccI CCATC 4 cut(s) 75, 194, 401, 629
BciT130I CCWGG 1 cut(s) 87
BciVI GTATCC 1 cut(s) 74
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 137, 151
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 2 cut(s) 228, 474
BfuI GTATCC 1 cut(s) 74
BglI GCCNNNNNGGC 1 cut(s) 588
BlnI CCTAGG 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 402, 649
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 4 cut(s) 92, 188, 310, 685
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 1 cut(s) 135
Bpu10I CCTNAGC 2 cut(s) 293, 549
BsaBI GATNNNNATC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 85, 227
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 128
Bse1I ACTGG 2 cut(s) 285, 542
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 85, 227
BseGI GGATG 2 cut(s) 179, 289
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 2 cut(s) 92, 128
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 2 cut(s) 285, 542
BseRI GAGGAG 1 cut(s) 449
BseSI GKGCMC 1 cut(s) 257
BseYI CCCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 295
BshFI GGCC 3 cut(s) 512, 582, 663
BshNI GGYRCC 1 cut(s) 647
BslI CCNNNNNNNGG 2 cut(s) 92, 128
BsmAI GTCTC 2 cut(s) 137, 151
BsnI GGCC 3 cut(s) 512, 582, 663
Bso31I GGTCTC 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 123, 257
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 325
BspANI GGCC 3 cut(s) 512, 582, 663
BspCNI CTCAG 1 cut(s) 285
BspLI GGNNCC 2 cut(s) 402, 649
BspT107I GGYRCC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 285, 542
BssECI CCNNGG 2 cut(s) 85, 227
BssMI GATC 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 4 cut(s) 8, 454, 544, 607
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 223, 257
BstDEI CTNAG 3 cut(s) 98, 293, 549
BstF5I GGATG 2 cut(s) 179, 289
BstKTI GATC 1 cut(s) 328
BstMAI GTCTC 2 cut(s) 137, 151
BstMBI GATC 1 cut(s) 325
BstMWI GCNNNNNNNGC 1 cut(s) 588
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 85
BstSLI GKGCMC 1 cut(s) 257
BstV2I GAAGAC 1 cut(s) 60
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 3 cut(s) 512, 582, 663
BtsCI GGATG 2 cut(s) 179, 289
BtsIMutI CAGTG 1 cut(s) 278
Cac8I GCNNGC 2 cut(s) 223, 257
Csp6I GTAC 2 cut(s) 4, 648
CviAII CATG 4 cut(s) 124, 222, 329, 514
CviJI RGCY 9 cut(s) 65, 121, 297, 512, 548, 582, 591, 663, 722
CviKI_1 RGCY 9 cut(s) 65, 121, 297, 512, 548, 582, 591, 663, 722
CviQI GTAC 2 cut(s) 4, 648
DdeI CTNAG 3 cut(s) 98, 293, 549
DpnI GATC 1 cut(s) 327
DpnII GATC 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 4 cut(s) 127, 225, 332, 517
FatI CATG 4 cut(s) 123, 221, 328, 513
FbaI TGATCA 1 cut(s) 325
FokI GGATG 2 cut(s) 166, 296
FriOI GRGCYC 1 cut(s) 123
FspBI CTAG 2 cut(s) 228, 474
GsaI CCCAGC 1 cut(s) 131
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 3 cut(s) 512, 582, 663
Hin1II CATG 4 cut(s) 127, 225, 332, 517
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 3 cut(s) 56, 110, 560
HphI GGTGA 2 cut(s) 181, 460
Hpy166II GTNNAC 2 cut(s) 199, 289
Hpy188I TCNGA 2 cut(s) 109, 424
Hpy188III TCNNGA 1 cut(s) 114
Hpy8I GTNNAC 2 cut(s) 199, 289
HpyCH4III ACNGT 4 cut(s) 8, 454, 544, 607
HpyCH4IV ACGT 3 cut(s) 21, 201, 381
HpyCH4V TGCA 4 cut(s) 179, 312, 630, 669
HpyF10VI GCNNNNNNNGC 1 cut(s) 588
HpyF3I CTNAG 3 cut(s) 98, 293, 549
HpySE526I ACGT 3 cut(s) 21, 201, 381
Hsp92II CATG 4 cut(s) 127, 225, 332, 517
KpnI GGTACC 1 cut(s) 651
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 1 cut(s) 325
LmnI GCTCC 1 cut(s) 264
LweI GCATC 4 cut(s) 92, 188, 310, 685
MaeI CTAG 2 cut(s) 228, 474
MaeII ACGT 3 cut(s) 21, 201, 381
MaeIII GTNAC 1 cut(s) 448
MalI GATC 1 cut(s) 327
MboI GATC 1 cut(s) 325
MboII GAAGA 1 cut(s) 65
MhlI GDGCHC 2 cut(s) 123, 257
MlsI TGGCCA 1 cut(s) 512
MluCI AATT 2 cut(s) 674, 712
MluNI TGGCCA 1 cut(s) 512
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 7 cut(s) 348, 385, 391, 427, 562, 674, 730
Mox20I TGGCCA 1 cut(s) 512
MscI TGGCCA 1 cut(s) 512
MseI TTAA 3 cut(s) 167, 534, 573
Msp20I TGGCCA 1 cut(s) 512
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 588
NdeII GATC 1 cut(s) 325
NlaIII CATG 4 cut(s) 127, 225, 332, 517
NlaIV GGNNCC 2 cut(s) 402, 649
NmuCI GTSAC 1 cut(s) 448
NspI RCATGY 1 cut(s) 225
PaeI GCATGC 1 cut(s) 225
PfeI GAWTC 2 cut(s) 110, 560
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PsiI TTATAA 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 2 cut(s) 402, 649
RsaI GTAC 2 cut(s) 5, 649
RsaNI GTAC 2 cut(s) 4, 648
SaqAI TTAA 3 cut(s) 167, 534, 573
Sau3AI GATC 1 cut(s) 325
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 2 cut(s) 123, 257
SfaNI GCATC 4 cut(s) 92, 188, 310, 685
SpeI ACTAGT 1 cut(s) 473
SphI GCATGC 1 cut(s) 225
Sse9I AATT 2 cut(s) 674, 712
SspI AATATT 1 cut(s) 244
SspMI CTAG 2 cut(s) 228, 474
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 227
TaaI ACNGT 4 cut(s) 8, 454, 544, 607
TaiI ACGT 3 cut(s) 24, 204, 384
TaqI TCGA 2 cut(s) 51, 690
TasI AATT 2 cut(s) 674, 712
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 2 cut(s) 110, 560
Tru1I TTAA 3 cut(s) 167, 534, 573
Tru9I TTAA 3 cut(s) 167, 534, 573
TscAI CASTG 1 cut(s) 285
TseFI GTSAC 1 cut(s) 448
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 3 cut(s) 203, 489, 530
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 1 cut(s) 285
XceI RCATGY 1 cut(s) 225
XcmI CCANNNNNNNNNTGG 1 cut(s) 74
XmaJI CCTAGG 1 cut(s) 227
XspI CTAG 2 cut(s) 228, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.