RchiOBHm_Chr7g0186021

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000786
Physical Location & Seq
Reverse (-)
6033306 .. 6034127
822 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16606

Sequence Viewer

Length: 822 bp
ATGTCATCACCTACCTCTTACCTCCTCATAGCTCTTCCGTTGCTCTTCTTCTTTAACTTCCATGGAAGCAAAGCCAACTTTTTGGCCTCAACAAATTGCCCCATATACACATGTGGTGATAATGAGCTTGATTTTCACTACCCTTTTTGGAAAATCGAAGACTCCACAGCCCATCAACATTGTGGATACCCTGGTTTTGGTCTAACATGCTCAGATTCTGGAGAGCCTATACTGACTCTACCAAATGATTCATGCATTGTTAAAGACATAAACTTTACTACATCCACCATCACCCTTGTTGACATTGACGTTGTTAATCAAACATGCCCCAGGGCCAGACACAGTATGAGTGTAGGCACGCTCCCATTGGATTACTCCCCATTGGATGTGAACCTGAGCTTCTACTTCAACTGCACTTCCTTTCCTGATCCTGTTGTGCCTCCCATAACCTGCTTGGGATCGCACAGTACCAAAAAGTCTTATGTGATCACGGAGGGAGAGGAGCCAGATGGGTTTGAGTGGTCGGAATATTGTGAGGAGAATGTGGTGGTGACTGTGAAAAAAACAGAGGAGATTACTAGAAGTATTGGTGAGTTGATTGGTGCATTTGGATTGGCCATGAACAATGGGTTTGTGCTGAACTGGACCATGGCTAAGGAGTGCGGTTCCTGTGAGACTAATGGTGGGTTCTGTGGCTACAACAACACTGCGCCAGAGATTCTGTGCTTTTGCAAAGATGGTAGTACTGGTACCACAAGCAATGGCCTCTGCAAGAAAGAGAAAGGTGTGGTTTCTTACCTTGATCCTTTTTTTAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

273

Amino Acids

30.01

Weight (kDa)

4.6

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 101 5.8e-15 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 150 - 247 5.2e-20 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 458
Acc65I GGTACC 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 749
AciI CCGC 1 cut(s) 663
AclWI GGATC 3 cut(s) 422, 466, 797
AcoI YGGCCR 1 cut(s) 615
AfaI GTAC 3 cut(s) 469, 745, 751
AfiI CCNNNNNNNGG 1 cut(s) 197
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 1 cut(s) 409
AjnI CCWGG 2 cut(s) 190, 329
AluBI AGCT 3 cut(s) 32, 127, 399
AluI AGCT 3 cut(s) 32, 127, 399
Alw26I GTCTC 1 cut(s) 668
AlwI GGATC 3 cut(s) 422, 466, 797
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 4 cut(s) 84, 333, 615, 763
Asp718I GGTACC 1 cut(s) 749
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 2 cut(s) 333, 645
AsuHPI GGTGA 4 cut(s) 128, 283, 562, 602
AvaII GGWCC 1 cut(s) 645
BaeI ACNNNNGTAYC 2 cut(s) 178, 211
BalI TGGCCA 1 cut(s) 617
BanI GGYRCC 1 cut(s) 749
BbsI GAAGAC 1 cut(s) 165
BccI CCATC 4 cut(s) 180, 296, 503, 731
BciT130I CCWGG 2 cut(s) 192, 331
BciVI GTATCC 1 cut(s) 179
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 1 cut(s) 668
BfaI CTAG 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 458
BfuI GTATCC 1 cut(s) 179
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 2 cut(s) 192, 331
Bme18I GGWCC 1 cut(s) 645
BmgT120I GGNCC 2 cut(s) 333, 645
BmiI GGNNCC 3 cut(s) 504, 667, 751
BmrFI CCNGG 2 cut(s) 192, 331
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 2 cut(s) 395, 654
BsaJI CCNNGG 5 cut(s) 61, 190, 329, 330, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse1I ACTGG 2 cut(s) 647, 751
Bse3DI GCAATG 1 cut(s) 766
BseBI CCWGG 2 cut(s) 192, 331
BseDI CCNNGG 5 cut(s) 61, 190, 329, 330, 648
BseGI GGATG 2 cut(s) 281, 391
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 1 cut(s) 766
BseMII CTCAG 2 cut(s) 225, 386
BseNI ACTGG 2 cut(s) 647, 751
BseRI GAGGAG 4 cut(s) 14, 515, 551, 584
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 4 cut(s) 86, 335, 617, 765
BshNI GGYRCC 1 cut(s) 749
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 668
BsnI GGCC 4 cut(s) 86, 335, 617, 765
Bsp143I GATC 4 cut(s) 427, 458, 486, 802
Bsp19I CCATGG 2 cut(s) 61, 648
BspACI CCGC 1 cut(s) 663
BspANI GGCC 4 cut(s) 86, 335, 617, 765
BspCNI CTCAG 2 cut(s) 224, 387
BspLI GGNNCC 3 cut(s) 504, 667, 751
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 3 cut(s) 422, 466, 797
BspQI GCTCTTC 2 cut(s) 39, 50
BspT107I GGYRCC 1 cut(s) 749
BsrDI GCAATG 1 cut(s) 766
BsrI ACTGG 2 cut(s) 647, 751
BssECI CCNNGG 5 cut(s) 61, 190, 329, 330, 648
BssMI GATC 4 cut(s) 427, 458, 486, 802
BssT1I CCWWGG 2 cut(s) 61, 648
Bst2UI CCWGG 2 cut(s) 192, 331
Bst4CI ACNGT 3 cut(s) 344, 467, 556
Bst6I CTCTTC 2 cut(s) 39, 50
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 3 cut(s) 211, 395, 654
BstDSI CCRYGG 2 cut(s) 61, 648
BstF5I GGATG 2 cut(s) 281, 391
BstHHI GCGC 1 cut(s) 712
BstKTI GATC 4 cut(s) 430, 461, 489, 805
BstMAI GTCTC 1 cut(s) 668
BstMBI GATC 4 cut(s) 427, 458, 486, 802
BstNI CCWGG 2 cut(s) 192, 331
BstNSI RCATGY 3 cut(s) 114, 210, 327
BstSCI CCNGG 2 cut(s) 190, 329
BstV2I GAAGAC 1 cut(s) 165
BstXI CCANNNNNNTGG 1 cut(s) 82
BsuI GTATCC 1 cut(s) 179
BsuRI GGCC 4 cut(s) 86, 335, 617, 765
BtgI CCRYGG 2 cut(s) 61, 648
BtsCI GGATG 2 cut(s) 281, 391
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 1 cut(s) 705
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 1 cut(s) 359
CaiI CAGNNNCTG 1 cut(s) 218
CfoI GCGC 1 cut(s) 712
Cfr13I GGNCC 2 cut(s) 333, 645
Csp6I GTAC 3 cut(s) 468, 744, 750
CviAII CATG 7 cut(s) 62, 111, 207, 252, 324, 619, 649
CviQI GTAC 3 cut(s) 468, 744, 750
DdeI CTNAG 3 cut(s) 211, 395, 654
DpnI GATC 4 cut(s) 429, 460, 488, 804
DpnII GATC 4 cut(s) 427, 458, 486, 802
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 2 cut(s) 39, 50
EarI CTCTTC 2 cut(s) 39, 50
Eco130I CCWWGG 2 cut(s) 61, 648
Eco47I GGWCC 1 cut(s) 645
EcoRII CCWGG 2 cut(s) 190, 329
EcoT14I CCWWGG 2 cut(s) 61, 648
EcoT22I ATGCAT 1 cut(s) 257
ErhI CCWWGG 2 cut(s) 61, 648
FaeI CATG 7 cut(s) 65, 114, 210, 255, 327, 622, 652
FatI CATG 7 cut(s) 61, 110, 206, 251, 323, 618, 648
FbaI TGATCA 1 cut(s) 486
FokI GGATG 2 cut(s) 268, 398
FspBI CTAG 1 cut(s) 579
GlaI GCGC 1 cut(s) 711
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 4 cut(s) 86, 335, 617, 765
HhaI GCGC 1 cut(s) 712
Hin1II CATG 7 cut(s) 65, 114, 210, 255, 327, 622, 652
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 5 cut(s) 161, 215, 235, 248, 718
HphI GGTGA 4 cut(s) 128, 283, 562, 602
Hpy166II GTNNAC 2 cut(s) 301, 391
Hpy188I TCNGA 2 cut(s) 214, 526
Hpy188III TCNNGA 2 cut(s) 219, 425
Hpy8I GTNNAC 2 cut(s) 301, 391
HpyCH4III ACNGT 3 cut(s) 344, 467, 556
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 5 cut(s) 255, 414, 605, 732, 771
HpyF3I CTNAG 3 cut(s) 211, 395, 654
HpySE526I ACGT 1 cut(s) 309
Hsp92II CATG 7 cut(s) 65, 114, 210, 255, 327, 622, 652
HspAI GCGC 1 cut(s) 710
KpnI GGTACC 1 cut(s) 753
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 4 cut(s) 427, 458, 486, 802
LguI GCTCTTC 2 cut(s) 39, 50
LmnI GCTCC 2 cut(s) 366, 502
MaeI CTAG 1 cut(s) 579
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 550
MalI GATC 4 cut(s) 429, 460, 488, 804
MboI GATC 4 cut(s) 427, 458, 486, 802
MboII GAAGA 4 cut(s) 26, 37, 40, 170
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 1 cut(s) 94
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 2 cut(s) 155, 229
MmeI TCCRAC 1 cut(s) 504
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 1 cut(s) 257
MscI TGGCCA 1 cut(s) 617
MseI TTAA 4 cut(s) 54, 261, 315, 813
Msp20I TGGCCA 1 cut(s) 617
MspR9I CCNGG 2 cut(s) 192, 331
MvaI CCWGG 2 cut(s) 192, 331
NcoI CCATGG 2 cut(s) 61, 648
NdeII GATC 4 cut(s) 427, 458, 486, 802
NlaIII CATG 7 cut(s) 65, 114, 210, 255, 327, 622, 652
NlaIV GGNNCC 3 cut(s) 504, 667, 751
NmuCI GTSAC 1 cut(s) 550
NsiI ATGCAT 1 cut(s) 257
NspI RCATGY 3 cut(s) 114, 210, 327
PasI CCCWGGG 1 cut(s) 330
PciI ACATGT 1 cut(s) 110
PciSI GCTCTTC 2 cut(s) 39, 50
PfeI GAWTC 3 cut(s) 215, 248, 718
PleI GAGTC 2 cut(s) 155, 229
PpsI GAGTC 2 cut(s) 155, 229
PscI ACATGT 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 190, 329
PspGI CCWGG 2 cut(s) 190, 329
PspN4I GGNNCC 3 cut(s) 504, 667, 751
PspPI GGNCC 2 cut(s) 333, 645
PstNI CAGNNNCTG 1 cut(s) 218
RsaI GTAC 3 cut(s) 469, 745, 751
RsaNI GTAC 3 cut(s) 468, 744, 750
SapI GCTCTTC 2 cut(s) 39, 50
SaqAI TTAA 4 cut(s) 54, 261, 315, 813
Sau3AI GATC 4 cut(s) 427, 458, 486, 802
Sau96I GGNCC 2 cut(s) 333, 645
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 2 cut(s) 155, 229
ScrFI CCNGG 2 cut(s) 192, 331
SinI GGWCC 1 cut(s) 645
Sse9I AATT 1 cut(s) 94
SsiI CCGC 1 cut(s) 663
SspI AATATT 1 cut(s) 530
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 2 cut(s) 190, 329
StyI CCWWGG 2 cut(s) 61, 648
TaaI ACNGT 3 cut(s) 344, 467, 556
TaiI ACGT 1 cut(s) 312
TaqI TCGA 1 cut(s) 156
TasI AATT 1 cut(s) 94
TatI WGTACW 1 cut(s) 743
TfiI GAWTC 3 cut(s) 215, 248, 718
Tru1I TTAA 4 cut(s) 54, 261, 315, 813
Tru9I TTAA 4 cut(s) 54, 261, 315, 813
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 1 cut(s) 550
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 2 cut(s) 240, 635
TspGWI ACGGA 2 cut(s) 27, 506
TspRI CASTG 1 cut(s) 712
VpaK11BI GGWCC 1 cut(s) 645
XceI RCATGY 3 cut(s) 114, 210, 327
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 451
XspI CTAG 1 cut(s) 579
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.