Rh7AG251700

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000786
Physical Location & Seq
Reverse (-)
25123769 .. 25128579
4811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG251700.1

Sequence Viewer

Length: 780 bp
ATGTCATCACCTATCTCTCACCTCCTTGCAGCTCTTCTTTCACTCTTGTTATTCAACTTCCATGGAAGCAAAGCCGACTTTATACCCACAAAAAATTGCCCCATATACAATTGTGGTAATGGCCCTGATTTTCACTACCCTTTTTGGAAAATTGAAGACTCCACCGCCGATCAATATTGTGGTTACCCTGGTTTTGGTCTCAATTGCTCAGAGTCCGGAGAGCCAATACTAAGTCTCCCAAATAATGACTCCTACTATGTGAAAGCCATAAACTTTATTAATTCCACCATGACCCTTGTTGACCTCGACATCCTCGATCAACAATGCCCTAGGGCAAGACACAGTGTTAGTGTAGGCACGCTCCCATTGGATTATTCCCCACTGGATGTGAACCTGAGCTTCTACTTCAACTGCACTTCCTTTCCTGATCCTATTGTGCCTCCCATAATCTGCTTGGGAATATGGGGTACCAAAATGTCTTATGTTATTACAGAGGGAGAGCAATCAGATGGGTTTGATTGGTCTGAAAGTTGTGAGGAGAATGTGGTGGTGACTGTGATGAAGAATAAGGAGCTTTATATTGATGAGTTGATTGGTGCTTTTGGAGGACTTATGAACCAGGGGTTTGTGCTTAACTGGACCACGGCGAAGGAGTGCGCTTCGTGTGAGAGTGGTGGTGGTTTGTGTGGGTACAACCCCACTAAAGCTGATGAGTTTCTCTGCTTCTGCAAATATGGCAATGGCCTATGCAGGAAGAAAGGTATGCTCCTTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

259

Amino Acids

28.54

Weight (kDa)

4.57

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 101 1.8e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 158 - 244 4.5e-18 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 1 cut(s) 165
AclWI GGATC 1 cut(s) 422
AfaI GTAC 2 cut(s) 469, 692
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 3 cut(s) 55, 155, 409
AjnI CCWGG 2 cut(s) 187, 618
AluBI AGCT 4 cut(s) 32, 399, 574, 707
AluI AGCT 4 cut(s) 32, 399, 574, 707
Alw26I GTCTC 2 cut(s) 203, 239
AlwI GGATC 1 cut(s) 422
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 2 cut(s) 121, 742
ApeKI GCWGC 1 cut(s) 29
AseI ATTAAT 1 cut(s) 279
Asp718I GGTACC 1 cut(s) 467
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 2 cut(s) 122, 639
AsuHPI GGTGA 2 cut(s) 11, 562
AvaII GGWCC 1 cut(s) 639
AvrII CCTAGG 1 cut(s) 329
BanI GGYRCC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 41
BccI CCATC 1 cut(s) 503
BceAI ACGGC 1 cut(s) 660
BciT130I CCWGG 2 cut(s) 189, 620
BcoDI GTCTC 2 cut(s) 203, 239
BfaI CTAG 1 cut(s) 330
BisI GCNGC 1 cut(s) 30
BlnI CCTAGG 1 cut(s) 329
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 2 cut(s) 189, 620
Bme18I GGWCC 1 cut(s) 639
BmgT120I GGNCC 2 cut(s) 122, 639
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 189, 620
BpiI GAAGAC 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 5 cut(s) 61, 187, 329, 619, 642
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 387, 641
Bse3DI GCAATG 1 cut(s) 745
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 2 cut(s) 189, 620
BseDI CCNNGG 5 cut(s) 61, 187, 329, 619, 642
BseGI GGATG 2 cut(s) 309, 391
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 745
BseMII CTCAG 2 cut(s) 222, 386
BseNI ACTGG 2 cut(s) 387, 641
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 2 cut(s) 123, 744
BshNI GGYRCC 1 cut(s) 467
BsiSI CCGG 1 cut(s) 216
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 2 cut(s) 203, 239
BsnI GGCC 2 cut(s) 123, 744
Bso31I GGTCTC 1 cut(s) 203
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 3 cut(s) 169, 316, 427
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 1 cut(s) 165
BspANI GGCC 2 cut(s) 123, 744
BspCNI CTCAG 2 cut(s) 221, 387
BspEI TCCGGA 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 469
BspPI GGATC 1 cut(s) 422
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 745
BsrI ACTGG 2 cut(s) 387, 641
BssECI CCNNGG 5 cut(s) 61, 187, 329, 619, 642
BssMI GATC 3 cut(s) 169, 316, 427
BssT1I CCWWGG 2 cut(s) 61, 329
Bst2UI CCWGG 2 cut(s) 189, 620
Bst4CI ACNGT 2 cut(s) 344, 556
Bst6I CTCTTC 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 3 cut(s) 208, 230, 395
BstDSI CCRYGG 2 cut(s) 61, 642
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 2 cut(s) 309, 391
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 3 cut(s) 172, 319, 430
BstMAI GTCTC 2 cut(s) 203, 239
BstMBI GATC 3 cut(s) 169, 316, 427
BstMWI GCNNNNNNNGC 1 cut(s) 735
BstNI CCWGG 2 cut(s) 189, 620
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 187, 618
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 162
BsuRI GGCC 2 cut(s) 123, 744
BtgI CCRYGG 2 cut(s) 61, 642
BtsCI GGATG 2 cut(s) 309, 391
BtsIMutI CAGTG 2 cut(s) 349, 380
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 659
Cfr13I GGNCC 2 cut(s) 122, 639
Csp6I GTAC 2 cut(s) 468, 691
CviAII CATG 2 cut(s) 62, 289
CviJI RGCY 9 cut(s) 32, 74, 123, 223, 266, 399, 574, 707, 744
CviKI_1 RGCY 9 cut(s) 32, 74, 123, 223, 266, 399, 574, 707, 744
CviQI GTAC 2 cut(s) 468, 691
DdeI CTNAG 3 cut(s) 208, 230, 395
DpnI GATC 3 cut(s) 171, 318, 429
DpnII GATC 3 cut(s) 169, 316, 427
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 2 cut(s) 61, 329
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 639
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 187, 618
EcoT14I CCWWGG 2 cut(s) 61, 329
ErhI CCWWGG 2 cut(s) 61, 329
FaeI CATG 2 cut(s) 65, 292
FatI CATG 2 cut(s) 61, 288
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 296, 398
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 658
GluI GCNGC 1 cut(s) 30
HaeIII GGCC 2 cut(s) 123, 744
HapII CCGG 1 cut(s) 216
HhaI GCGC 1 cut(s) 659
Hin1II CATG 2 cut(s) 65, 292
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 3 cut(s) 158, 212, 248
HpaII CCGG 1 cut(s) 216
HphI GGTGA 2 cut(s) 11, 562
Hpy166II GTNNAC 2 cut(s) 301, 391
Hpy188I TCNGA 3 cut(s) 211, 508, 526
Hpy188III TCNNGA 2 cut(s) 216, 425
Hpy8I GTNNAC 2 cut(s) 301, 391
HpyAV CCTTC 1 cut(s) 643
HpyCH4III ACNGT 2 cut(s) 344, 556
HpyCH4V TGCA 4 cut(s) 29, 414, 729, 750
HpyF10VI GCNNNNNNNGC 1 cut(s) 735
HpyF3I CTNAG 3 cut(s) 208, 230, 395
Hsp92II CATG 2 cut(s) 65, 292
HspAI GCGC 1 cut(s) 657
Kpn2I TCCGGA 1 cut(s) 215
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 3 cut(s) 169, 316, 427
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 366, 571, 771
Lsp1109I GCAGC 1 cut(s) 41
MaeI CTAG 1 cut(s) 330
MaeIII GTNAC 2 cut(s) 182, 550
MalI GATC 3 cut(s) 171, 318, 429
MboI GATC 3 cut(s) 169, 316, 427
MboII GAAGA 4 cut(s) 26, 167, 574, 766
MfeI CAATTG 2 cut(s) 109, 202
MluCI AATT 5 cut(s) 94, 109, 150, 202, 280
MlyI GAGTC 3 cut(s) 152, 221, 242
MnlI CCTC 7 cut(s) 32, 314, 323, 450, 487, 529, 599
MroI TCCGGA 1 cut(s) 215
MseI TTAA 2 cut(s) 279, 633
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 2 cut(s) 189, 620
MunI CAATTG 2 cut(s) 109, 202
MvaI CCWGG 2 cut(s) 189, 620
MwoI GCNNNNNNNGC 1 cut(s) 735
NcoI CCATGG 1 cut(s) 61
NdeII GATC 3 cut(s) 169, 316, 427
NlaIII CATG 2 cut(s) 65, 292
NlaIV GGNNCC 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 550
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 3 cut(s) 152, 220, 242
PpsI GAGTC 3 cut(s) 152, 220, 242
PshBI ATTAAT 1 cut(s) 279
Psp6I CCWGG 2 cut(s) 187, 618
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 187, 618
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 2 cut(s) 122, 639
RsaI GTAC 2 cut(s) 469, 692
RsaNI GTAC 2 cut(s) 468, 691
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 279, 633
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 3 cut(s) 169, 316, 427
Sau96I GGNCC 2 cut(s) 122, 639
SchI GAGTC 3 cut(s) 152, 221, 242
ScrFI CCNGG 2 cut(s) 189, 620
SetI ASST 9 cut(s) 13, 24, 34, 306, 396, 401, 576, 709, 763
SinI GGWCC 1 cut(s) 639
Sse9I AATT 5 cut(s) 94, 109, 150, 202, 280
SsiI CCGC 1 cut(s) 165
SspI AATATT 1 cut(s) 176
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 187, 618
StyI CCWWGG 2 cut(s) 61, 329
TaaI ACNGT 2 cut(s) 344, 556
TaqI TCGA 2 cut(s) 306, 315
TasI AATT 5 cut(s) 94, 109, 150, 202, 280
Tru1I TTAA 2 cut(s) 279, 633
Tru9I TTAA 2 cut(s) 279, 633
TscAI CASTG 2 cut(s) 349, 387
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 2 cut(s) 575, 629
TspRI CASTG 2 cut(s) 349, 387
VpaK11BI GGWCC 1 cut(s) 639
VspI ATTAAT 1 cut(s) 279
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmaJI CCTAGG 1 cut(s) 329
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.