RchiOBHm_Chr7g0185941

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000786
Physical Location & Seq
Forward (+)
5993237 .. 5996222
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16598

Sequence Viewer

Length: 1110 bp
ATGTCATCTGCTCGTTTGTACCTCCTTGCAGCTCTTCTCTCACTCTTGTTATTCAACTTTCATGGAAGCAAAGCCCACTTTGTACCCTCAACAAATTGCCCCATATACAATTGCGGTAAGGATCTGAAGTTTCACTACCCTTTTTGGAAAATTGAAGACTCCACCGCCGGTCAATATTGTGGTTACCCTGGTTTTGGTCTCAATTGCTCAGAGTCCGGAGAGCCAATACTCAGTCTCCCAAATAATGACTCCTACCATGTGAAAGACATAAACTTTATTGATTCCACCATCACCCTTGTTGACATCGACGTCTTCGATCAACAATGCCCTAGGGCAAGACACAGTATTAGTGTAGGCACGCTCCCATTGGATTACTCCCCATTGGATGTGAACCTGAGCTTCTACTTTAACTGCACTTCCTTTCCTGATCCTGTTGTGCCTCCCATAATCTGCTTGGGAATATGGGGTACCAAAATGTCTTATGTTTTCACGGAGGGAGAGCAATCAGATGGGTTTGAATGGTCTGAAAGTTGTGAGGAGAATGTGGTGGTGACTGTGATGAAGAGTAAGAAGCTTTATATTGATAAGTTGATTGGTGCTTTTGGAGGACTTATGAACCAGGGGTTTGTGCTTAACTGGACCACGGCGAAGGAGTGCGCTTCGTGTGAGAGTGGTGGTGGTTTGTGTGGGTACAACCCCACTAAAGCTGATGAGTTTCTCTGCTTCTGCAAATATAGTGATGGCCTATGCGAGAAGAAAGGCACTAGGTTCAACTGGAGATTGAAGGTTGTCATAGGCGTAGGAACTGGTATCCTAGTGTTTGTTATTCTCTTCCGTGGGATTTCACTGATTAGGGAAAATGACAATGAAGATGCCGAGGCCTTGACTCAGAACATTGGACCTCTAGCAGTAAAAAGATACAAATTTTCGGATGTAACGAAAATGACCAACTCATTTAAAGATAAAATAGGTCAAGGCGGTTATGGTGACGTGTACGAAGGTAAGCTATTGAATGGTTGTCGTGTGGCTGTGAAGGTTCTGAAAGCATCCAAAGGGAATGGAGAGGTCTTTGTTAATGAGGTTGCAAGCATTAGTAGAACTTCCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

369

Amino Acids

40.73

Weight (kDa)

5.92

Isoelectric Point (pI)

28.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 101 9.9e-16 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 158 - 244 1.2e-17 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AatII GACGTC 1 cut(s) 312
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccIII TCCGGA 1 cut(s) 215
AciI CCGC 3 cut(s) 114, 165, 978
AclWI GGATC 2 cut(s) 129, 422
AcsI RAATTY 1 cut(s) 923
AcuI CTGAAG 1 cut(s) 146
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 5 cut(s) 20, 84, 469, 692, 995
AfiI CCNNNNNNNGG 1 cut(s) 194
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 6 cut(s) 55, 155, 518, 772, 784, 1012
AjiI CACGTC 1 cut(s) 991
AjnI CCWGG 2 cut(s) 187, 618
AluBI AGCT 5 cut(s) 32, 399, 574, 707, 1006
AluI AGCT 5 cut(s) 32, 399, 574, 707, 1006
Alw26I GTCTC 2 cut(s) 203, 239
AlwI GGATC 2 cut(s) 129, 422
Aor13HI TCCGGA 1 cut(s) 215
AoxI GGCC 2 cut(s) 742, 879
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 923
Asp718I GGTACC 1 cut(s) 467
AspA2I CCTAGG 1 cut(s) 329
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 2 cut(s) 639, 899
AsuHPI GGTGA 3 cut(s) 283, 562, 998
AvaII GGWCC 2 cut(s) 639, 899
AvrII CCTAGG 1 cut(s) 329
BanI GGYRCC 1 cut(s) 467
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbsI GAAGAC 2 cut(s) 162, 304
BbvI GCAGC 1 cut(s) 41
BccI CCATC 3 cut(s) 296, 503, 734
BceAI ACGGC 1 cut(s) 660
BciT130I CCWGG 2 cut(s) 189, 620
BciVI GTATCC 1 cut(s) 821
BcoDI GTCTC 2 cut(s) 203, 239
BfaI CTAG 4 cut(s) 330, 765, 815, 905
BfuI GTATCC 1 cut(s) 821
BisI GCNGC 1 cut(s) 30
BlnI CCTAGG 1 cut(s) 329
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 2 cut(s) 189, 620
Bme18I GGWCC 2 cut(s) 639, 899
BmgBI CACGTC 1 cut(s) 991
BmgT120I GGNCC 2 cut(s) 639, 899
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 189, 620
BmsI GCATC 2 cut(s) 862, 1055
BpiI GAAGAC 2 cut(s) 162, 304
BpmI CTGGAG 1 cut(s) 796
Bpu10I CCTNAGC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 6 cut(s) 187, 329, 619, 642, 835, 876
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 219, 249
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 3 cut(s) 641, 779, 811
BseAI TCCGGA 1 cut(s) 215
BseBI CCWGG 2 cut(s) 189, 620
BseDI CCNNGG 6 cut(s) 187, 329, 619, 642, 835, 876
BseGI GGATG 3 cut(s) 391, 937, 1046
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 4 cut(s) 222, 244, 386, 902
BseNI ACTGG 3 cut(s) 641, 779, 811
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 397
BshFI GGCC 2 cut(s) 744, 881
BshNI GGYRCC 1 cut(s) 467
BsiSI CCGG 2 cut(s) 168, 216
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 2 cut(s) 203, 239
BsnI GGCC 2 cut(s) 744, 881
Bso31I GGTCTC 1 cut(s) 203
Bsp13I TCCGGA 1 cut(s) 215
Bsp143I GATC 3 cut(s) 121, 316, 427
BspACI CCGC 3 cut(s) 114, 165, 978
BspANI GGCC 2 cut(s) 744, 881
BspCNI CTCAG 4 cut(s) 221, 243, 387, 901
BspEI TCCGGA 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 469
BspPI GGATC 2 cut(s) 129, 422
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 467
BspTNI GGTCTC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 3 cut(s) 641, 779, 811
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 6 cut(s) 187, 329, 619, 642, 835, 876
BssMI GATC 3 cut(s) 121, 316, 427
BssNI GRCGYC 1 cut(s) 309
BssT1I CCWWGG 1 cut(s) 329
Bst2UI CCWGG 2 cut(s) 189, 620
Bst4CI ACNGT 2 cut(s) 344, 556
Bst6I CTCTTC 3 cut(s) 39, 557, 836
BstACI GRCGYC 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 359, 1087
BstDEI CTNAG 4 cut(s) 208, 230, 395, 888
BstDSI CCRYGG 2 cut(s) 642, 835
BstEII GGTNACC 1 cut(s) 182
BstF5I GGATG 3 cut(s) 391, 937, 1046
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 3 cut(s) 124, 319, 430
BstMAI GTCTC 2 cut(s) 203, 239
BstMBI GATC 3 cut(s) 121, 316, 427
BstNI CCWGG 2 cut(s) 189, 620
BstPI GGTNACC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 187, 618
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 2 cut(s) 162, 304
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuI GTATCC 1 cut(s) 821
BsuRI GGCC 2 cut(s) 744, 881
BtgI CCRYGG 2 cut(s) 642, 835
BtrI CACGTC 1 cut(s) 991
BtsCI GGATG 3 cut(s) 391, 937, 1046
BtsIMutI CAGTG 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 359, 1087
CfoI GCGC 1 cut(s) 659
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 639, 899
Csp6I GTAC 5 cut(s) 19, 83, 468, 691, 994
CviAII CATG 2 cut(s) 62, 257
CviQI GTAC 5 cut(s) 19, 83, 468, 691, 994
DdeI CTNAG 4 cut(s) 208, 230, 395, 888
DpnI GATC 3 cut(s) 123, 318, 429
DpnII GATC 3 cut(s) 121, 316, 427
DraI TTTAAA 1 cut(s) 958
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 3 cut(s) 39, 557, 836
EarI CTCTTC 3 cut(s) 39, 557, 836
Eco130I CCWWGG 1 cut(s) 329
Eco147I AGGCCT 1 cut(s) 881
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 639, 899
Eco57I CTGAAG 1 cut(s) 146
Eco91I GGTNACC 1 cut(s) 182
EcoO65I GGTNACC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 187, 618
EcoT14I CCWWGG 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 2 cut(s) 65, 260
FatI CATG 2 cut(s) 61, 256
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 3 cut(s) 398, 944, 1033
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 4 cut(s) 330, 765, 815, 905
GlaI GCGC 1 cut(s) 658
GluI GCNGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 796
HaeIII GGCC 2 cut(s) 744, 881
HapII CCGG 2 cut(s) 168, 216
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 2 cut(s) 65, 260
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 5 cut(s) 158, 212, 248, 281, 886
HpaII CCGG 2 cut(s) 168, 216
HphI GGTGA 3 cut(s) 283, 562, 998
Hpy166II GTNNAC 3 cut(s) 301, 391, 994
Hpy188I TCNGA 7 cut(s) 126, 211, 508, 526, 891, 931, 1041
Hpy188III TCNNGA 2 cut(s) 216, 425
Hpy8I GTNNAC 3 cut(s) 301, 391, 994
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 4 cut(s) 643, 778, 992, 1027
HpyCH4III ACNGT 2 cut(s) 344, 556
HpyCH4IV ACGT 2 cut(s) 309, 990
HpyCH4V TGCA 4 cut(s) 29, 414, 729, 1085
HpyF3I CTNAG 4 cut(s) 208, 230, 395, 888
HpySE526I ACGT 2 cut(s) 309, 990
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 2 cut(s) 65, 260
HspAI GCGC 1 cut(s) 657
Kpn2I TCCGGA 1 cut(s) 215
KpnI GGTACC 1 cut(s) 471
Kzo9I GATC 3 cut(s) 121, 316, 427
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 1 cut(s) 366
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 2 cut(s) 862, 1055
MaeI CTAG 4 cut(s) 330, 765, 815, 905
MaeII ACGT 2 cut(s) 309, 990
MaeIII GTNAC 4 cut(s) 182, 550, 934, 986
MalI GATC 3 cut(s) 123, 318, 429
MboI GATC 3 cut(s) 121, 316, 427
MboII GAAGA 7 cut(s) 26, 167, 304, 574, 766, 823, 881
MfeI CAATTG 2 cut(s) 109, 202
MflI RGATCY 1 cut(s) 121
MluCI AATT 5 cut(s) 94, 109, 150, 202, 923
MlyI GAGTC 4 cut(s) 152, 221, 242, 880
MroI TCCGGA 1 cut(s) 215
MseI TTAA 5 cut(s) 408, 633, 957, 1074, 1108
MspI CCGG 2 cut(s) 168, 216
MspR9I CCNGG 2 cut(s) 189, 620
MunI CAATTG 2 cut(s) 109, 202
MvaI CCWGG 2 cut(s) 189, 620
NdeII GATC 3 cut(s) 121, 316, 427
NlaIII CATG 2 cut(s) 65, 260
NlaIV GGNNCC 1 cut(s) 469
NmeAIII GCCGAG 1 cut(s) 901
NmuCI GTSAC 2 cut(s) 550, 986
PceI AGGCCT 1 cut(s) 881
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 312, 935
PfeI GAWTC 1 cut(s) 281
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 4 cut(s) 152, 220, 242, 880
PpsI GAGTC 4 cut(s) 152, 220, 242, 880
Psp6I CCWGG 2 cut(s) 187, 618
PspEI GGTNACC 1 cut(s) 182
PspGI CCWGG 2 cut(s) 187, 618
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 2 cut(s) 639, 899
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 5 cut(s) 20, 84, 469, 692, 995
RsaNI GTAC 5 cut(s) 19, 83, 468, 691, 994
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 5 cut(s) 408, 633, 957, 1074, 1108
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 3 cut(s) 121, 316, 427
Sau96I GGNCC 2 cut(s) 639, 899
SchI GAGTC 4 cut(s) 152, 221, 242, 880
ScrFI CCNGG 2 cut(s) 189, 620
SfaNI GCATC 2 cut(s) 862, 1055
SinI GGWCC 2 cut(s) 639, 899
Sse9I AATT 5 cut(s) 94, 109, 150, 202, 923
SseBI AGGCCT 1 cut(s) 881
SsiI CCGC 3 cut(s) 114, 165, 978
SspI AATATT 1 cut(s) 176
SspMI CTAG 4 cut(s) 330, 765, 815, 905
StuI AGGCCT 1 cut(s) 881
StyD4I CCNGG 2 cut(s) 187, 618
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 2 cut(s) 344, 556
TaiI ACGT 2 cut(s) 312, 993
TaqI TCGA 2 cut(s) 306, 315
TasI AATT 5 cut(s) 94, 109, 150, 202, 923
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 5 cut(s) 408, 633, 957, 1074, 1108
Tru9I TTAA 5 cut(s) 408, 633, 957, 1074, 1108
TscAI CASTG 1 cut(s) 852
TseFI GTSAC 2 cut(s) 550, 986
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 2 cut(s) 550, 986
TspDTI ATGAA 4 cut(s) 50, 575, 629, 882
TspGWI ACGGA 2 cut(s) 506, 824
TspRI CASTG 1 cut(s) 852
VpaK11BI GGWCC 2 cut(s) 639, 899
XapI RAATTY 1 cut(s) 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XmaJI CCTAGG 1 cut(s) 329
XspI CTAG 4 cut(s) 330, 765, 815, 905
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.