Rh7DG077400

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000786
Physical Location & Seq
Forward (+)
6120148 .. 6120999
852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG077400.1

Sequence Viewer

Length: 852 bp
ATGTCATCTACTCGTTTGTACCTCCTTGCAGCTGTTCTCTTACTCTTGTTCTTCAACTTCCATGGAAGCAATGCTAATGCTAGCTTTTCAGCCTCAACAAATTGCCCCATGTACAGTTGTGGTAATGACGTTTATTTTCACTACCCTTTTTGGAAAATCGAAGACTCCACAGCCCATCAACATTGTGGATACCCTGGTTTTGGTCTAAGATGCTCGGATTCTGGAGAGCCCATGCTGGGTCTCCCAAACAATGTCTCCTACTATGTGAAAGACTTAAACTTTATTGCATCCACCATCACCCTTGTTGACGTTGATGTTGTTCATCAAGCATGCCCTAGGGCAAGACACAATATTAGTTTGGGCACGCTCCCATTGGATTACTCCCCACTGGATGTGAACCTGAGCTTCTACTTCAACTGCACTTTAGATGCTGATCATGTTGTGCCTCCTATAACCTGTTTGGGAATATATGGTATCAAAAAGTCTTACGTTTTCACGGAGGGAGAGGAACCAGATGGGTTTGATTGGTCTGAAAGTTGTGAGGAGAATGTGGTGGTGACTGTGAAAGAAACAGAGATTACTAGTAGTATTGATGAGTTGATTGGTTCATTTGGAGTGGCCATGAACAATGGGTTTGTGCTTAACTGGACTGTGGCTAAGGAGTGCGATTCCTGTGAGGTTAATGGTGGCCTTTGTGGCTACAAGAACCTTACTGTGCCAGAGTTTCTGTGCTTTTGCAGAGATGGTAGTATTGGTACCAGAAACAATGGCCTCTGCAAGAAATTAGGTATGTTTGTCGATGATGCTCTCTCTTTATCTAATTATAAAGCTATGGGTCTGTTTGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

283

Amino Acids

31.18

Weight (kDa)

4.62

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 103 2.1e-15 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 159 - 249 6.6e-19 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 825
Acc65I GGTACC 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 618
AfaI GTAC 3 cut(s) 20, 113, 757
AfiI CCNNNNNNNGG 2 cut(s) 200, 236
AgsI TTSAA 2 cut(s) 55, 415
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 4 cut(s) 32, 84, 405, 830
AluI AGCT 4 cut(s) 32, 84, 405, 830
Alw26I GTCTC 2 cut(s) 245, 259
AoxI GGCC 3 cut(s) 618, 688, 769
ApeKI GCWGC 1 cut(s) 29
Asp718I GGTACC 1 cut(s) 755
AspA2I CCTAGG 1 cut(s) 335
AsuHPI GGTGA 2 cut(s) 289, 568
AsuNHI GCTAGC 1 cut(s) 80
AvrII CCTAGG 1 cut(s) 335
BaeGI GKGCMC 1 cut(s) 365
BaeI ACNNNNGTAYC 2 cut(s) 181, 214
BalI TGGCCA 1 cut(s) 620
BanI GGYRCC 1 cut(s) 755
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 41
BccI CCATC 4 cut(s) 183, 302, 509, 737
BciT130I CCWGG 1 cut(s) 195
BciVI GTATCC 1 cut(s) 182
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 2 cut(s) 245, 259
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 3 cut(s) 81, 336, 582
BfuI GTATCC 1 cut(s) 182
BglI GCCNNNNNGGC 1 cut(s) 696
BisI GCNGC 1 cut(s) 30
BlnI CCTAGG 1 cut(s) 335
BlsI GCNGC 1 cut(s) 31
Bme1390I CCNGG 1 cut(s) 195
BmiI GGNNCC 2 cut(s) 510, 757
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 4 cut(s) 200, 296, 418, 793
BmtI GCTAGC 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 2 cut(s) 401, 657
BsaBI GATNNNNATC 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 61, 193, 335
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 236
Bse1I ACTGG 2 cut(s) 393, 650
Bse3DI GCAATG 1 cut(s) 76
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 3 cut(s) 61, 193, 335
BseGI GGATG 2 cut(s) 287, 397
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 200, 236
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 1 cut(s) 392
BseNI ACTGG 2 cut(s) 393, 650
BseRI GAGGAG 1 cut(s) 557
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 1 cut(s) 41
BseYI CCCAGC 1 cut(s) 235
BsgI GTGCAG 1 cut(s) 403
BshFI GGCC 3 cut(s) 620, 690, 771
BshNI GGYRCC 1 cut(s) 755
BslI CCNNNNNNNGG 2 cut(s) 200, 236
BsmAI GTCTC 2 cut(s) 245, 259
BsnI GGCC 3 cut(s) 620, 690, 771
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 231, 365
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 1 cut(s) 433
Bsp19I CCATGG 1 cut(s) 61
BspANI GGCC 3 cut(s) 620, 690, 771
BspCNI CTCAG 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 510, 757
BspOI GCTAGC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 755
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 2 cut(s) 393, 650
BssECI CCNNGG 3 cut(s) 61, 193, 335
BssMI GATC 1 cut(s) 433
BssT1I CCWWGG 2 cut(s) 61, 335
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 116, 562, 652, 715
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 82, 331, 365
BstDEI CTNAG 3 cut(s) 206, 401, 657
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 2 cut(s) 287, 397
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 2 cut(s) 245, 259
BstMBI GATC 1 cut(s) 433
BstMWI GCNNNNNNNGC 1 cut(s) 696
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 193
BstSLI GKGCMC 1 cut(s) 365
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 168
BsuI GTATCC 1 cut(s) 182
BsuRI GGCC 3 cut(s) 620, 690, 771
BtgI CCRYGG 1 cut(s) 61
BtsCI GGATG 2 cut(s) 287, 397
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 3 cut(s) 82, 331, 365
Csp6I GTAC 3 cut(s) 19, 112, 756
CviAII CATG 6 cut(s) 62, 109, 232, 330, 437, 622
CviQI GTAC 3 cut(s) 19, 112, 756
DdeI CTNAG 3 cut(s) 206, 401, 657
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 618
Eco130I CCWWGG 2 cut(s) 61, 335
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 61, 335
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 2 cut(s) 61, 335
FaeI CATG 6 cut(s) 65, 112, 235, 333, 440, 625
FatI CATG 6 cut(s) 61, 108, 231, 329, 436, 621
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 2 cut(s) 274, 404
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 1 cut(s) 30
FspBI CTAG 3 cut(s) 81, 336, 582
GluI GCNGC 1 cut(s) 30
GsaI CCCAGC 1 cut(s) 239
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 3 cut(s) 620, 690, 771
Hin1II CATG 6 cut(s) 65, 112, 235, 333, 440, 625
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 3 cut(s) 164, 218, 668
HphI GGTGA 2 cut(s) 289, 568
Hpy166II GTNNAC 2 cut(s) 307, 397
Hpy188I TCNGA 2 cut(s) 217, 532
Hpy188III TCNNGA 1 cut(s) 222
Hpy8I GTNNAC 2 cut(s) 307, 397
HpyCH4III ACNGT 4 cut(s) 116, 562, 652, 715
HpyCH4IV ACGT 3 cut(s) 129, 309, 489
HpyCH4V TGCA 5 cut(s) 29, 287, 420, 738, 777
HpyF10VI GCNNNNNNNGC 1 cut(s) 696
HpyF3I CTNAG 3 cut(s) 206, 401, 657
HpySE526I ACGT 3 cut(s) 129, 309, 489
Hsp92II CATG 6 cut(s) 65, 112, 235, 333, 440, 625
KpnI GGTACC 1 cut(s) 759
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 372
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 4 cut(s) 200, 296, 418, 793
MaeI CTAG 3 cut(s) 81, 336, 582
MaeII ACGT 3 cut(s) 129, 309, 489
MaeIII GTNAC 1 cut(s) 556
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 2 cut(s) 43, 173
MhlI GDGCHC 2 cut(s) 231, 365
MlsI TGGCCA 1 cut(s) 620
MluCI AATT 3 cut(s) 100, 782, 820
MluNI TGGCCA 1 cut(s) 620
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 9 cut(s) 32, 103, 456, 493, 499, 535, 670, 782, 838
Mox20I TGGCCA 1 cut(s) 620
MscI TGGCCA 1 cut(s) 620
MseI TTAA 3 cut(s) 275, 642, 681
Msp20I TGGCCA 1 cut(s) 620
MspA1I CMGCKG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 696
NcoI CCATGG 1 cut(s) 61
NdeII GATC 1 cut(s) 433
NheI GCTAGC 1 cut(s) 80
NlaIII CATG 6 cut(s) 65, 112, 235, 333, 440, 625
NlaIV GGNNCC 2 cut(s) 510, 757
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 333
PaeI GCATGC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 218, 668
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PsiI TTATAA 1 cut(s) 825
Psp6I CCWGG 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 235
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 2 cut(s) 510, 757
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 3 cut(s) 20, 113, 757
RsaNI GTAC 3 cut(s) 19, 112, 756
SaqAI TTAA 3 cut(s) 275, 642, 681
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 1 cut(s) 433
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 2 cut(s) 231, 365
SfaNI GCATC 4 cut(s) 200, 296, 418, 793
SpeI ACTAGT 1 cut(s) 581
SphI GCATGC 1 cut(s) 333
Sse9I AATT 3 cut(s) 100, 782, 820
SspI AATATT 1 cut(s) 352
SspMI CTAG 3 cut(s) 81, 336, 582
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 2 cut(s) 61, 335
TaaI ACNGT 4 cut(s) 116, 562, 652, 715
TaiI ACGT 3 cut(s) 132, 312, 492
TaqI TCGA 2 cut(s) 159, 798
TasI AATT 3 cut(s) 100, 782, 820
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 2 cut(s) 218, 668
Tru1I TTAA 3 cut(s) 275, 642, 681
Tru9I TTAA 3 cut(s) 275, 642, 681
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 3 cut(s) 311, 597, 638
TspGWI ACGGA 1 cut(s) 512
TspRI CASTG 1 cut(s) 393
XceI RCATGY 1 cut(s) 333
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmaJI CCTAGG 1 cut(s) 335
XspI CTAG 3 cut(s) 81, 336, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.