pycom06g19580

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000786
Physical Location & Seq
Reverse (-)
22949088 .. 22949876
789 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19580.1

Sequence Viewer

Length: 789 bp
ATGCCACCACTTGATGTTTACCACCTTGTTTCTTTCTCACTCTTCTTCTTGATCGTGAAGTCATATGGAATCAGCAACACTAGTAGTTCTTCATCCTCAACCAATATTTGCGGCACATATGATTGCGGCAACGGCTTAGCCTTTCGCTATCCGTTTTGGCATGTCGAAGTCGAAGCCACCACTGCCGATCAGTACTGTGGCTACCCTGGTTTCGGCCTCACATGCTCCGCCGACGGCGAGCCTATCCTCACCCTACCAACCGACTCATATTATGTCAAACAAATAAACTACACTAATTCCACCGTCCATCTTGTTGACATCGATGTCGTTGGCCATACATGCCCTAGGGCACGACATAATGTAACTCTAGGCACCCTCCCTTTAAATTACTCCCATCCAGATTTGAACCTCAGTTTTTTCTTCAACTGCACTTCCTACCCTCCTCTCGTGCCTCCCATTGGGTGTTTGGGATACGGTAAGATGAAGTCTTACGTGTTTACGGTGGGGAACGAGACGGAGGGCTTTGATTGGTTCGAGAACTGCGAGGAGAATGTGGTGGTTCCGGTGATAAAGACGACGGAGATTACTAGTGACTTTGGTGGTTTGATCGGTGGATTTGGTGGCGCTATGAACGAAGGGTTTGTGCTGGATTGGGGCATGGCTAAGGACTGCGGATCTTGTGAGGCAAATGGTGGTTTGTGTGGCTACAACAACACTGCGCACAATTTTCTGTGCTTTTGCAAAGATGAAACCAGAACCAACGGTGTCTGCAAACAAGGTATGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

263

Amino Acids

28.58

Weight (kDa)

4.74

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 106 1.3e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 162 - 249 4.5e-21 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 323
Acc16I TGCGCA 1 cut(s) 720
AccB1I GGYRCC 1 cut(s) 371
AciI CCGC 4 cut(s) 111, 126, 228, 672
AclWI GGATC 1 cut(s) 682
AcoI YGGCCR 1 cut(s) 331
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 212, 458
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 2 cut(s) 406, 424
AhlI ACTAGT 2 cut(s) 80, 587
AjnI CCWGG 1 cut(s) 205
Alw26I GTCTC 1 cut(s) 506
AlwI GGATC 1 cut(s) 682
AoxI GGCC 2 cut(s) 214, 331
AspA2I CCTAGG 1 cut(s) 344
AspLEI GCGC 2 cut(s) 626, 721
AsuHPI GGTGA 2 cut(s) 241, 577
AvrII CCTAGG 1 cut(s) 344
BaeGI GKGCMC 1 cut(s) 352
BalI TGGCCA 1 cut(s) 333
BanI GGYRCC 1 cut(s) 371
BauI CACGAG 1 cut(s) 446
BccI CCATC 2 cut(s) 315, 402
BceAI ACGGC 2 cut(s) 148, 250
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 464
BcoDI GTCTC 1 cut(s) 506
BcuI ACTAGT 2 cut(s) 80, 587
BfaI CTAG 4 cut(s) 81, 345, 368, 588
BfoI RGCGCY 1 cut(s) 627
BfuI GTATCC 1 cut(s) 464
BisI GCNGC 2 cut(s) 112, 127
BlnI CCTAGG 1 cut(s) 344
BlpI GCTNAGC 1 cut(s) 136
BlsI GCNGC 2 cut(s) 113, 128
BmcAI AGTACT 1 cut(s) 194
Bme1390I CCNGG 1 cut(s) 207
BmiI GGNNCC 2 cut(s) 373, 561
BmrFI CCNGG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 663
Bpu1102I GCTNAGC 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 321
BsaAI YACGTR 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 205, 344
BsaWI WCCGGW 1 cut(s) 562
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 458
BseBI CCWGG 1 cut(s) 207
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 205, 344
BseGI GGATG 2 cut(s) 92, 394
BseLI CCNNNNNNNGG 2 cut(s) 212, 458
BseMII CTCAG 1 cut(s) 424
BseRI GAGGAG 2 cut(s) 432, 560
BseSI GKGCMC 1 cut(s) 352
BsgI GTGCAG 1 cut(s) 412
BshFI GGCC 2 cut(s) 216, 333
BshNI GGYRCC 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 321
BsiSI CCGG 1 cut(s) 563
BslI CCNNNNNNNGG 2 cut(s) 212, 458
BsmAI GTCTC 1 cut(s) 506
BsmBI CGTCTC 1 cut(s) 506
BsnI GGCC 2 cut(s) 216, 333
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 4 cut(s) 51, 187, 606, 674
Bsp1720I GCTNAGC 1 cut(s) 136
BspACI CCGC 4 cut(s) 111, 126, 228, 672
BspANI GGCC 2 cut(s) 216, 333
BspCNI CTCAG 1 cut(s) 423
BspDI ATCGAT 1 cut(s) 321
BspLI GGNNCC 2 cut(s) 373, 561
BspPI GGATC 1 cut(s) 682
BspT107I GGYRCC 1 cut(s) 371
BssECI CCNNGG 2 cut(s) 205, 344
BssMI GATC 4 cut(s) 51, 187, 606, 674
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 5 cut(s) 197, 304, 476, 502, 764
Bst6I CTCTTC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 493
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 3 cut(s) 136, 410, 663
BstF5I GGATG 2 cut(s) 92, 394
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 2 cut(s) 626, 721
BstKTI GATC 4 cut(s) 54, 190, 609, 677
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 4 cut(s) 51, 187, 606, 674
BstMWI GCNNNNNNNGC 4 cut(s) 132, 182, 222, 339
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 3 cut(s) 164, 225, 342
BstSCI CCNGG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 674
BstYI RGATCY 1 cut(s) 674
Bsu15I ATCGAT 1 cut(s) 321
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 2 cut(s) 216, 333
BsuTUI ATCGAT 1 cut(s) 321
BtsCI GGATG 2 cut(s) 92, 394
BtsI GCAGTG 2 cut(s) 180, 714
BtsIMutI CAGTG 2 cut(s) 180, 714
Cac8I GCNNGC 1 cut(s) 239
CfoI GCGC 2 cut(s) 626, 721
ClaI ATCGAT 1 cut(s) 321
Csp6I GTAC 1 cut(s) 193
CviAII CATG 4 cut(s) 161, 222, 339, 658
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 3 cut(s) 136, 410, 663
DpnI GATC 4 cut(s) 53, 189, 608, 676
DpnII GATC 4 cut(s) 51, 187, 606, 674
DraI TTTAAA 1 cut(s) 384
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 344
ErhI CCWWGG 1 cut(s) 344
Esp3I CGTCTC 1 cut(s) 506
FaeI CATG 4 cut(s) 164, 225, 342, 661
FatI CATG 4 cut(s) 160, 221, 338, 657
FauNDI CATATG 2 cut(s) 64, 118
Fnu4HI GCNGC 2 cut(s) 112, 127
FokI GGATG 2 cut(s) 79, 381
Fsp4HI GCNGC 2 cut(s) 112, 127
FspBI CTAG 4 cut(s) 81, 345, 368, 588
FspI TGCGCA 1 cut(s) 720
GlaI GCGC 2 cut(s) 625, 720
GluI GCNGC 2 cut(s) 112, 127
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 2 cut(s) 216, 333
HapII CCGG 1 cut(s) 563
HhaI GCGC 2 cut(s) 626, 721
Hin1II CATG 4 cut(s) 164, 225, 342, 661
Hin6I GCGC 2 cut(s) 624, 719
HinP1I GCGC 2 cut(s) 624, 719
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 2 cut(s) 69, 263
HpaII CCGG 1 cut(s) 563
HphI GGTGA 2 cut(s) 241, 577
Hpy166II GTNNAC 3 cut(s) 19, 316, 498
Hpy188III TCNNGA 4 cut(s) 49, 55, 398, 535
Hpy8I GTNNAC 3 cut(s) 19, 316, 498
Hpy99I CGWCG 2 cut(s) 236, 580
HpyAV CCTTC 1 cut(s) 629
HpyCH4III ACNGT 5 cut(s) 197, 304, 476, 502, 764
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 3 cut(s) 429, 741, 771
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 182, 222, 339
HpyF3I CTNAG 3 cut(s) 136, 410, 663
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 4 cut(s) 164, 225, 342, 661
HspAI GCGC 2 cut(s) 624, 719
Kzo9I GATC 4 cut(s) 51, 187, 606, 674
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 6 cut(s) 192, 219, 411, 576, 632, 766
MaeI CTAG 4 cut(s) 81, 345, 368, 588
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 2 cut(s) 361, 590
MalI GATC 4 cut(s) 53, 189, 608, 676
MboI GATC 4 cut(s) 51, 187, 606, 674
MboII GAAGA 4 cut(s) 34, 37, 81, 412
MflI RGATCY 1 cut(s) 674
MhlI GDGCHC 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 3 cut(s) 295, 385, 724
MluNI TGGCCA 1 cut(s) 333
MlyI GAGTC 1 cut(s) 257
Mox20I TGGCCA 1 cut(s) 333
MscI TGGCCA 1 cut(s) 333
MseI TTAA 1 cut(s) 383
Msp20I TGGCCA 1 cut(s) 333
MspI CCGG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 4 cut(s) 132, 182, 222, 339
NdeI CATATG 2 cut(s) 64, 118
NdeII GATC 4 cut(s) 51, 187, 606, 674
NlaIII CATG 4 cut(s) 164, 225, 342, 661
NlaIV GGNNCC 2 cut(s) 373, 561
NmuCI GTSAC 1 cut(s) 590
NsbI TGCGCA 1 cut(s) 720
NspI RCATGY 3 cut(s) 164, 225, 342
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PfeI GAWTC 1 cut(s) 69
PkrI GCNGC 2 cut(s) 113, 128
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
Ppu21I YACGTR 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 373, 561
PsuI RGATCY 1 cut(s) 674
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 2 cut(s) 112, 127
Sau3AI GATC 4 cut(s) 51, 187, 606, 674
ScaI AGTACT 1 cut(s) 194
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 352
SetI ASST 4 cut(s) 27, 411, 495, 781
SpeI ACTAGT 2 cut(s) 80, 587
Sse9I AATT 3 cut(s) 295, 385, 724
SsiI CCGC 4 cut(s) 111, 126, 228, 672
SspI AATATT 1 cut(s) 106
SspMI CTAG 4 cut(s) 81, 345, 368, 588
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 5 cut(s) 197, 304, 476, 502, 764
TaiI ACGT 1 cut(s) 495
TaqI TCGA 4 cut(s) 165, 171, 321, 534
TasI AATT 3 cut(s) 295, 385, 724
TatI WGTACW 1 cut(s) 192
TauI GCSGC 2 cut(s) 114, 129
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 2 cut(s) 187, 721
TseFI GTSAC 1 cut(s) 590
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 4 cut(s) 81, 497, 644, 762
TspGWI ACGGA 3 cut(s) 141, 530, 593
TspRI CASTG 2 cut(s) 187, 721
XceI RCATGY 3 cut(s) 164, 225, 342
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmaJI CCTAGG 1 cut(s) 344
XspI CTAG 4 cut(s) 81, 345, 368, 588
ZrmI AGTACT 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.