Rw7G006440

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000786
Physical Location & Seq
Forward (+)
5498952 .. 5499847
896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006440.1

Sequence Viewer

Length: 861 bp
ATGTCATCTGCTCGTTTGTACCTCCTTGTAGCTGTTCTCTTACCCTTGTTCTTCAACTTCCATGGAAGCAATGCTAATGCTAGCTTTTCAGCCTCAACAAATTGCCCCATGTACAGTTGTGGTAATGAGGTTTATTTTCACTACCCTTTTTGGAAAATCGAAGACTCCACAGCCCATCAACATTGTGGATACCCTGGTTTTGGTCTAAGATGCTCGGATTCTGGAGAGCCCATGCTGGGTCTCCCAAACAATGTCTCCTACTATGTGAAAGACTTAAACTTTATTGCATCCACCATCACCCTTGTTGAGTTGGCAAGACACAATATTAGTTTGGGCACGCTCCCATTGGATTACTCCCCACTGGATGTGAACCTGAGCTTCTACTTCAACTGCACTTTAGATGCTGATCATGTTGTGCCTCCTATAACCTGTTTGGGAATATATGGTACCAAAAAGTCTTACGTTTTCACGGAGGGAGAGGAACCAGATGGGTTTGATTGGTCTGAAAGTTGTGAGGAGAATGTGGTGGTGACTGTGAAAGAAACAGAGATTACTAGTAGTATTGATGAGTTGATTGGTTCATTTGGAGTGGCCATGAACAATGGGTTTGTGCTTAACTGGACCGTGGCTAAGGAGTGCGGTTCCTGTGAGGTTAATGGTGGCCTGTGTGGCTACAACAACCTTACTGTGCCAGAGTTTCTGTGCTTTTGCAGAGATGATAGTATTGGTACCAGAAGCAATGGCCTCTGCAAGAAATTAGGTATGTTTGTCGATGATGCTCTCTCTTTATCTAATTATAAAGCTATGGATCTCTTATTGAATGGTTGCAAAACAAGTAAATGCTATATACCTGCTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

286

Amino Acids

31.4

Weight (kDa)

4.65

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 102 5.1e-15 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 144 - 239 6.8e-19 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
Acc65I GGTACC 2 cut(s) 446, 728
AccB1I GGYRCC 2 cut(s) 446, 728
AciI CCGC 1 cut(s) 639
AclWI GGATC 1 cut(s) 816
AcoI YGGCCR 1 cut(s) 591
AfaI GTAC 4 cut(s) 20, 113, 448, 730
AfiI CCNNNNNNNGG 2 cut(s) 200, 236
AgsI TTSAA 3 cut(s) 55, 388, 820
AhlI ACTAGT 1 cut(s) 554
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 4 cut(s) 32, 84, 378, 803
AluI AGCT 4 cut(s) 32, 84, 378, 803
Alw26I GTCTC 2 cut(s) 245, 259
AlwI GGATC 1 cut(s) 816
AoxI GGCC 3 cut(s) 591, 661, 742
Asp718I GGTACC 2 cut(s) 446, 728
AspS9I GGNCC 1 cut(s) 621
AsuHPI GGTGA 2 cut(s) 289, 541
AsuNHI GCTAGC 1 cut(s) 80
AvaII GGWCC 1 cut(s) 621
BaeGI GKGCMC 1 cut(s) 338
BaeI ACNNNNGTAYC 2 cut(s) 181, 214
BalI TGGCCA 1 cut(s) 593
BanI GGYRCC 2 cut(s) 446, 728
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 168
BccI CCATC 3 cut(s) 183, 302, 482
BciT130I CCWGG 1 cut(s) 195
BciVI GTATCC 1 cut(s) 182
BclI TGATCA 1 cut(s) 406
BcoDI GTCTC 2 cut(s) 245, 259
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 2 cut(s) 81, 555
BfuI GTATCC 1 cut(s) 182
BglI GCCNNNNNGGC 1 cut(s) 669
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 621
BmiI GGNNCC 4 cut(s) 448, 483, 643, 730
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 4 cut(s) 200, 296, 391, 766
BmtI GCTAGC 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 2 cut(s) 374, 630
BsaBI GATNNNNATC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 61, 193, 624
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 236
Bse1I ACTGG 2 cut(s) 366, 623
Bse3DI GCAATG 2 cut(s) 76, 745
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 3 cut(s) 61, 193, 624
BseGI GGATG 2 cut(s) 287, 370
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 2 cut(s) 200, 236
BseMI GCAATG 2 cut(s) 76, 745
BseMII CTCAG 1 cut(s) 365
BseNI ACTGG 2 cut(s) 366, 623
BseRI GAGGAG 1 cut(s) 530
BseSI GKGCMC 1 cut(s) 338
BseYI CCCAGC 1 cut(s) 235
BsgI GTGCAG 1 cut(s) 376
BshFI GGCC 3 cut(s) 593, 663, 744
BshNI GGYRCC 2 cut(s) 446, 728
BslI CCNNNNNNNGG 2 cut(s) 200, 236
BsmAI GTCTC 2 cut(s) 245, 259
BsnI GGCC 3 cut(s) 593, 663, 744
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 231, 338
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 406, 808
Bsp19I CCATGG 1 cut(s) 61
BspACI CCGC 1 cut(s) 639
BspANI GGCC 3 cut(s) 593, 663, 744
BspCNI CTCAG 1 cut(s) 366
BspLI GGNNCC 4 cut(s) 448, 483, 643, 730
BspOI GCTAGC 1 cut(s) 84
BspPI GGATC 1 cut(s) 816
BspT107I GGYRCC 2 cut(s) 446, 728
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 2 cut(s) 76, 745
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 2 cut(s) 366, 623
BssECI CCNNGG 3 cut(s) 61, 193, 624
BssMI GATC 2 cut(s) 406, 808
BssT1I CCWWGG 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 116, 535, 625, 688
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 82, 338
BstDEI CTNAG 3 cut(s) 206, 374, 630
BstDSI CCRYGG 2 cut(s) 61, 624
BstF5I GGATG 2 cut(s) 287, 370
BstKTI GATC 2 cut(s) 409, 811
BstMAI GTCTC 2 cut(s) 245, 259
BstMBI GATC 2 cut(s) 406, 808
BstMWI GCNNNNNNNGC 1 cut(s) 669
BstNI CCWGG 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 193
BstSLI GKGCMC 1 cut(s) 338
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
BsuI GTATCC 1 cut(s) 182
BsuRI GGCC 3 cut(s) 593, 663, 744
BtgI CCRYGG 2 cut(s) 61, 624
BtsCI GGATG 2 cut(s) 287, 370
BtsIMutI CAGTG 1 cut(s) 359
Cac8I GCNNGC 2 cut(s) 82, 338
Cfr13I GGNCC 1 cut(s) 621
Csp6I GTAC 4 cut(s) 19, 112, 447, 729
CviAII CATG 5 cut(s) 62, 109, 232, 410, 595
CviQI GTAC 4 cut(s) 19, 112, 447, 729
DdeI CTNAG 3 cut(s) 206, 374, 630
DpnI GATC 2 cut(s) 408, 810
DpnII GATC 2 cut(s) 406, 808
EaeI YGGCCR 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 1 cut(s) 621
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 61
FaeI CATG 5 cut(s) 65, 112, 235, 413, 598
FatI CATG 5 cut(s) 61, 108, 231, 409, 594
FbaI TGATCA 1 cut(s) 406
FokI GGATG 2 cut(s) 274, 377
FriOI GRGCYC 1 cut(s) 231
FspBI CTAG 2 cut(s) 81, 555
GsaI CCCAGC 1 cut(s) 239
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 3 cut(s) 593, 663, 744
Hin1II CATG 5 cut(s) 65, 112, 235, 413, 598
HinfI GANTC 2 cut(s) 164, 218
HphI GGTGA 2 cut(s) 289, 541
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 2 cut(s) 217, 505
Hpy188III TCNNGA 1 cut(s) 222
Hpy8I GTNNAC 1 cut(s) 370
HpyCH4III ACNGT 4 cut(s) 116, 535, 625, 688
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 5 cut(s) 287, 393, 711, 750, 828
HpyF10VI GCNNNNNNNGC 1 cut(s) 669
HpyF3I CTNAG 3 cut(s) 206, 374, 630
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 5 cut(s) 65, 112, 235, 413, 598
KpnI GGTACC 2 cut(s) 450, 732
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 2 cut(s) 406, 808
LmnI GCTCC 1 cut(s) 345
LweI GCATC 4 cut(s) 200, 296, 391, 766
MaeI CTAG 2 cut(s) 81, 555
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 1 cut(s) 529
MalI GATC 2 cut(s) 408, 810
MboI GATC 2 cut(s) 406, 808
MboII GAAGA 2 cut(s) 43, 173
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 2 cut(s) 231, 338
MlsI TGGCCA 1 cut(s) 593
MluCI AATT 3 cut(s) 100, 755, 793
MluNI TGGCCA 1 cut(s) 593
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 9 cut(s) 32, 103, 121, 429, 466, 472, 508, 643, 755
Mox20I TGGCCA 1 cut(s) 593
MscI TGGCCA 1 cut(s) 593
MseI TTAA 3 cut(s) 275, 615, 654
Msp20I TGGCCA 1 cut(s) 593
MspR9I CCNGG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 669
NcoI CCATGG 1 cut(s) 61
NdeII GATC 2 cut(s) 406, 808
NheI GCTAGC 1 cut(s) 80
NlaIII CATG 5 cut(s) 65, 112, 235, 413, 598
NlaIV GGNNCC 4 cut(s) 448, 483, 643, 730
NmuCI GTSAC 1 cut(s) 529
PfeI GAWTC 1 cut(s) 218
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PsiI TTATAA 1 cut(s) 798
Psp6I CCWGG 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 235
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 4 cut(s) 448, 483, 643, 730
PspPI GGNCC 1 cut(s) 621
PsuI RGATCY 1 cut(s) 808
RsaI GTAC 4 cut(s) 20, 113, 448, 730
RsaNI GTAC 4 cut(s) 19, 112, 447, 729
SaqAI TTAA 3 cut(s) 275, 615, 654
Sau3AI GATC 2 cut(s) 406, 808
Sau96I GGNCC 1 cut(s) 621
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 2 cut(s) 231, 338
SfaNI GCATC 4 cut(s) 200, 296, 391, 766
SinI GGWCC 1 cut(s) 621
SpeI ACTAGT 1 cut(s) 554
Sse9I AATT 3 cut(s) 100, 755, 793
SsiI CCGC 1 cut(s) 639
SspI AATATT 1 cut(s) 325
SspMI CTAG 2 cut(s) 81, 555
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 4 cut(s) 116, 535, 625, 688
TaiI ACGT 1 cut(s) 465
TaqI TCGA 2 cut(s) 159, 771
TasI AATT 3 cut(s) 100, 755, 793
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 3 cut(s) 275, 615, 654
Tru9I TTAA 3 cut(s) 275, 615, 654
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 1 cut(s) 529
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 2 cut(s) 570, 611
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 1 cut(s) 366
VpaK11BI GGWCC 1 cut(s) 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XspI CTAG 2 cut(s) 81, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.