Rh7DG077600

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000786
Physical Location & Seq
Forward (+)
6138479 .. 6142739
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG077600.1

Sequence Viewer

Length: 1212 bp
ATGTCATCTGCTCGTTTGTACCTCCTTGTAGCTCTTCTCTTGCTCTTCTTCTTTAACTTCCATGGAAGCAAAGCCAACTTTTTGGCCTCAACAAATTGCCCCATATACAGATGTGATAATGAGCTTGATTTTCACTACCCTTTTTGGAAAATCGAAGACTCCACAGCCCATCAACATTGTGGATACCCTGGTTTTGGTCTAACATGCTCAGATTCTGGAGAGCCTATACTGACTCTACCAAATGATTCATTCATTGTTAAAGACATAAACTTTCCTACATCCACCATCACCCTTGTTGACATTGACGTTGTTAATCAAACATGCCCCAGGGCAAGACACAGTATGAGTGTAGGCACGCTCCCATTGGATTACTCCCCATTGGATGTGAACCTGAGCTTCTACTTCAACTGCACTTCCTTTCCTGATCCTGTTGTGCCTCCCATAACCTGCTTGGGATCGCACGGTACCAAAAAGTCTTATGTGATCACGGAGGGAGAGGAGCCATATGGGTTTGAGTGGTCGGAATATTGTGCGGAGAATGTGGTGGTGACTGTGAAAAAAACAGAGGAGATTACTAGAAGTATTGGTGAGTTGATTGGTGCATTTGGATTGGCCATGAACAATGGGTTTGTGCTTAACTGGACCATGGATAAGGAGTGCGGTTCCTGTGAGACTAATGGTGGGTTCTGTGGCTACAACAACACTGCACCAGAGATTCTGTGCTTTTGCAAAGATGGTAGTATTGGTACCACAAGCAATGGCCTCTGCAAGAAATTAGGCACTAAGTTCAACTGGAGATTGAAGGTTGTCATAGGCGTAGTTGCTACTATTGCAGGAACTGGTATCCTAGTGTTTGTTATTCTCTTCCGTGGTATTTCACTGATTAGTGAAAATGACAATGGAGATGCCGAGGCCTTCATTCAGAACATTGGACCTCTAGCAGTAAAAAGATACATATTTTCGGATGGCAGCAATTTTGGATTGCTCAGTGCCGCGACAGAAGAGGAAAAGGAACTTGCGAAGAAGATGTTCTTAGTGGGATTGTGGTGCATACAGACAAAGCCATCAGATAGGCCATCCATGAGTAAAGTGATTGAAATGTTGGAAGGAAGCACTGAAGCCTTGCAAATACCACCAAAGCCTGTCCTATCTTCTCCTGTAAGATCACCAGCAGAATCTTCCACCTTATCAGTAGTATCTGTTCTAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

403

Amino Acids

44.05

Weight (kDa)

5.0

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 100 8.1e-15 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 149 - 246 3e-19 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 455
Acc65I GGTACC 2 cut(s) 464, 746
AccB1I GGYRCC 2 cut(s) 464, 746
AccII CGCG 1 cut(s) 995
AciI CCGC 3 cut(s) 533, 660, 993
AclWI GGATC 2 cut(s) 419, 463
AcoI YGGCCR 1 cut(s) 612
AcuI CTGAAG 1 cut(s) 1137
AfaI GTAC 3 cut(s) 20, 466, 748
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 4 cut(s) 406, 790, 802, 1097
AjnI CCWGG 2 cut(s) 187, 326
AluBI AGCT 3 cut(s) 32, 124, 396
AluI AGCT 3 cut(s) 32, 124, 396
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 2 cut(s) 419, 463
AlwNI CAGNNNCTG 2 cut(s) 215, 839
AoxI GGCC 5 cut(s) 84, 612, 760, 912, 1073
ApeKI GCWGC 1 cut(s) 969
Asp700I GAANNNNTTC 1 cut(s) 1028
Asp718I GGTACC 2 cut(s) 464, 746
AspS9I GGNCC 2 cut(s) 642, 932
AsuHPI GGTGA 4 cut(s) 280, 559, 599, 1158
AvaII GGWCC 2 cut(s) 642, 932
BaeI ACNNNNGTAYC 2 cut(s) 175, 208
BalI TGGCCA 1 cut(s) 614
BanI GGYRCC 2 cut(s) 464, 746
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 981
BccI CCATC 6 cut(s) 177, 293, 728, 959, 1072, 1084
BciT130I CCWGG 2 cut(s) 189, 328
BciVI GTATCC 2 cut(s) 176, 854
BclI TGATCA 1 cut(s) 483
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 4 cut(s) 576, 848, 938, 1205
BfuAI ACCTGC 1 cut(s) 455
BfuI GTATCC 2 cut(s) 176, 854
BisI GCNGC 2 cut(s) 970, 993
BlsI GCNGC 2 cut(s) 971, 994
Bme1390I CCNGG 2 cut(s) 189, 328
Bme18I GGWCC 2 cut(s) 642, 932
BmgT120I GGNCC 2 cut(s) 642, 932
BmiI GGNNCC 4 cut(s) 466, 501, 664, 748
BmrFI CCNGG 2 cut(s) 189, 328
BmsI GCATC 1 cut(s) 895
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 2 cut(s) 237, 814
Bpu10I CCTNAGC 1 cut(s) 392
BsaJI CCNNGG 7 cut(s) 61, 187, 326, 327, 645, 868, 909
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse1I ACTGG 3 cut(s) 644, 797, 844
Bse3DI GCAATG 1 cut(s) 763
BseBI CCWGG 2 cut(s) 189, 328
BseDI CCNNGG 7 cut(s) 61, 187, 326, 327, 645, 868, 909
BseGI GGATG 4 cut(s) 278, 388, 970, 1076
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 763
BseMII CTCAG 3 cut(s) 222, 383, 1000
BseNI ACTGG 3 cut(s) 644, 797, 844
BseRI GAGGAG 2 cut(s) 512, 581
BseXI GCAGC 1 cut(s) 981
BsgI GTGCAG 2 cut(s) 394, 690
Bsh1236I CGCG 1 cut(s) 995
BshFI GGCC 5 cut(s) 86, 614, 762, 914, 1075
BshNI GGYRCC 2 cut(s) 464, 746
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 665
BsnI GGCC 5 cut(s) 86, 614, 762, 914, 1075
Bsp143I GATC 4 cut(s) 424, 455, 483, 1163
Bsp19I CCATGG 2 cut(s) 61, 645
BspACI CCGC 3 cut(s) 533, 660, 993
BspANI GGCC 5 cut(s) 86, 614, 762, 914, 1075
BspCNI CTCAG 3 cut(s) 221, 384, 999
BspFNI CGCG 1 cut(s) 995
BspLI GGNNCC 4 cut(s) 466, 501, 664, 748
BspMI ACCTGC 1 cut(s) 455
BspPI GGATC 2 cut(s) 419, 463
BspQI GCTCTTC 2 cut(s) 39, 50
BspT107I GGYRCC 2 cut(s) 464, 746
BsrDI GCAATG 1 cut(s) 763
BsrI ACTGG 3 cut(s) 644, 797, 844
BssECI CCNNGG 7 cut(s) 61, 187, 326, 327, 645, 868, 909
BssMI GATC 4 cut(s) 424, 455, 483, 1163
BssT1I CCWWGG 2 cut(s) 61, 645
Bst2UI CCWGG 2 cut(s) 189, 328
Bst4CI ACNGT 3 cut(s) 341, 464, 553
Bst6I CTCTTC 4 cut(s) 39, 50, 869, 996
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 5 cut(s) 208, 392, 783, 986, 1033
BstDSI CCRYGG 3 cut(s) 61, 645, 868
BstF5I GGATG 4 cut(s) 278, 388, 970, 1076
BstFNI CGCG 1 cut(s) 995
BstKTI GATC 4 cut(s) 427, 458, 486, 1166
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 4 cut(s) 424, 455, 483, 1163
BstMWI GCNNNNNNNGC 1 cut(s) 830
BstNI CCWGG 2 cut(s) 189, 328
BstNSI RCATGY 2 cut(s) 207, 324
BstSCI CCNGG 2 cut(s) 187, 326
BstUI CGCG 1 cut(s) 995
BstV1I GCAGC 1 cut(s) 981
BstV2I GAAGAC 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 82
BsuI GTATCC 2 cut(s) 176, 854
BsuRI GGCC 5 cut(s) 86, 614, 762, 914, 1075
BtgI CCRYGG 3 cut(s) 61, 645, 868
BtsCI GGATG 4 cut(s) 278, 388, 970, 1076
BtsI GCAGTG 1 cut(s) 702
BtsIMutI CAGTG 4 cut(s) 702, 878, 994, 1113
BveI ACCTGC 1 cut(s) 455
Cac8I GCNNGC 1 cut(s) 356
CaiI CAGNNNCTG 2 cut(s) 215, 839
Cfr13I GGNCC 2 cut(s) 642, 932
Csp6I GTAC 3 cut(s) 19, 465, 747
CviAII CATG 6 cut(s) 62, 204, 321, 616, 646, 1081
CviQI GTAC 3 cut(s) 19, 465, 747
DdeI CTNAG 5 cut(s) 208, 392, 783, 986, 1033
DpnI GATC 4 cut(s) 426, 457, 485, 1165
DpnII GATC 4 cut(s) 424, 455, 483, 1163
EaeI YGGCCR 1 cut(s) 612
Eam1104I CTCTTC 4 cut(s) 39, 50, 869, 996
EarI CTCTTC 4 cut(s) 39, 50, 869, 996
Eco130I CCWWGG 2 cut(s) 61, 645
Eco147I AGGCCT 1 cut(s) 914
Eco47I GGWCC 2 cut(s) 642, 932
Eco57I CTGAAG 1 cut(s) 1137
EcoRII CCWGG 2 cut(s) 187, 326
EcoT14I CCWWGG 2 cut(s) 61, 645
ErhI CCWWGG 2 cut(s) 61, 645
FaeI CATG 6 cut(s) 65, 207, 324, 619, 649, 1084
FalI AAGNNNNNCTT 2 cut(s) 1016, 1048
FatI CATG 6 cut(s) 61, 203, 320, 615, 645, 1080
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 483
Fnu4HI GCNGC 2 cut(s) 970, 993
FokI GGATG 4 cut(s) 265, 395, 977, 1063
Fsp4HI GCNGC 2 cut(s) 970, 993
FspBI CTAG 4 cut(s) 576, 848, 938, 1205
GluI GCNGC 2 cut(s) 970, 993
GsuI CTGGAG 2 cut(s) 237, 814
HaeIII GGCC 5 cut(s) 86, 614, 762, 914, 1075
Hin1II CATG 6 cut(s) 65, 207, 324, 619, 649, 1084
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HinfI GANTC 6 cut(s) 158, 212, 232, 245, 715, 1175
HphI GGTGA 4 cut(s) 280, 559, 599, 1158
Hpy166II GTNNAC 2 cut(s) 298, 388
Hpy188I TCNGA 5 cut(s) 211, 523, 924, 964, 1069
Hpy188III TCNNGA 3 cut(s) 216, 422, 1205
Hpy8I GTNNAC 2 cut(s) 298, 388
HpyAV CCTTC 3 cut(s) 796, 925, 1100
HpyCH4III ACNGT 3 cut(s) 341, 464, 553
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 8 cut(s) 411, 602, 707, 729, 768, 833, 1050, 1126
HpyF10VI GCNNNNNNNGC 1 cut(s) 830
HpyF3I CTNAG 5 cut(s) 208, 392, 783, 986, 1033
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 6 cut(s) 65, 207, 324, 619, 649, 1084
KpnI GGTACC 2 cut(s) 468, 750
Ksp22I TGATCA 1 cut(s) 483
Kzo9I GATC 4 cut(s) 424, 455, 483, 1163
LguI GCTCTTC 2 cut(s) 39, 50
LmnI GCTCC 2 cut(s) 363, 499
Lsp1109I GCAGC 1 cut(s) 981
LweI GCATC 1 cut(s) 895
MaeI CTAG 4 cut(s) 576, 848, 938, 1205
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 547
MalI GATC 4 cut(s) 426, 457, 485, 1165
MboI GATC 4 cut(s) 424, 455, 483, 1163
MlsI TGGCCA 1 cut(s) 614
MluCI AATT 3 cut(s) 94, 773, 973
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 2 cut(s) 152, 226
MmeI TCCRAC 2 cut(s) 501, 1083
Mox20I TGGCCA 1 cut(s) 614
MroXI GAANNNNTTC 1 cut(s) 1028
MscI TGGCCA 1 cut(s) 614
MseI TTAA 5 cut(s) 54, 258, 312, 636, 1210
Msp20I TGGCCA 1 cut(s) 614
MspR9I CCNGG 2 cut(s) 189, 328
MvaI CCWGG 2 cut(s) 189, 328
MvnI CGCG 1 cut(s) 995
MwoI GCNNNNNNNGC 1 cut(s) 830
NcoI CCATGG 2 cut(s) 61, 645
NdeI CATATG 1 cut(s) 505
NdeII GATC 4 cut(s) 424, 455, 483, 1163
NlaIII CATG 6 cut(s) 65, 207, 324, 619, 649, 1084
NlaIV GGNNCC 4 cut(s) 466, 501, 664, 748
NmeAIII GCCGAG 1 cut(s) 934
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 2 cut(s) 207, 324
PasI CCCWGGG 1 cut(s) 327
PceI AGGCCT 1 cut(s) 914
PciSI GCTCTTC 2 cut(s) 39, 50
PdmI GAANNNNTTC 1 cut(s) 1028
PfeI GAWTC 4 cut(s) 212, 245, 715, 1175
PkrI GCNGC 2 cut(s) 971, 994
PleI GAGTC 2 cut(s) 152, 226
PpsI GAGTC 2 cut(s) 152, 226
Psp6I CCWGG 2 cut(s) 187, 326
PspGI CCWGG 2 cut(s) 187, 326
PspN4I GGNNCC 4 cut(s) 466, 501, 664, 748
PspPI GGNCC 2 cut(s) 642, 932
PsrI GAACNNNNNNTAC 1 cut(s) 1185
PstNI CAGNNNCTG 2 cut(s) 215, 839
RsaI GTAC 3 cut(s) 20, 466, 748
RsaNI GTAC 3 cut(s) 19, 465, 747
SapI GCTCTTC 2 cut(s) 39, 50
SaqAI TTAA 5 cut(s) 54, 258, 312, 636, 1210
SatI GCNGC 2 cut(s) 970, 993
Sau3AI GATC 4 cut(s) 424, 455, 483, 1163
Sau96I GGNCC 2 cut(s) 642, 932
SchI GAGTC 2 cut(s) 152, 226
ScrFI CCNGG 2 cut(s) 189, 328
SfaNI GCATC 1 cut(s) 895
SinI GGWCC 2 cut(s) 642, 932
Sse9I AATT 3 cut(s) 94, 773, 973
SseBI AGGCCT 1 cut(s) 914
SsiI CCGC 3 cut(s) 533, 660, 993
SspI AATATT 1 cut(s) 527
SspMI CTAG 4 cut(s) 576, 848, 938, 1205
StuI AGGCCT 1 cut(s) 914
StyD4I CCNGG 2 cut(s) 187, 326
StyI CCWWGG 2 cut(s) 61, 645
TaaI ACNGT 3 cut(s) 341, 464, 553
TaiI ACGT 1 cut(s) 309
TaqI TCGA 1 cut(s) 153
TasI AATT 3 cut(s) 94, 773, 973
TauI GCSGC 1 cut(s) 995
TfiI GAWTC 4 cut(s) 212, 245, 715, 1175
Tru1I TTAA 5 cut(s) 54, 258, 312, 636, 1210
Tru9I TTAA 5 cut(s) 54, 258, 312, 636, 1210
TscAI CASTG 4 cut(s) 709, 885, 994, 1120
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 969
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 4 cut(s) 237, 241, 632, 907
TspGWI ACGGA 2 cut(s) 503, 857
TspRI CASTG 4 cut(s) 709, 885, 994, 1120
VpaK11BI GGWCC 2 cut(s) 642, 932
XbaI TCTAGA 1 cut(s) 1204
XceI RCATGY 2 cut(s) 207, 324
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 448
XmnI GAANNNNTTC 1 cut(s) 1028
XspI CTAG 4 cut(s) 576, 848, 938, 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.