MD09G1093300.v1.1

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000678
Physical Location & Seq
Reverse (-)
6818039 .. 6823135
5097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1093300.v1.1.491

Sequence Viewer

Length: 2103 bp
ATGCTACATTCGCAATTCTCATATCAATATGCCGATTCTCAGTTCCAGAAATTTGAATCCATTTCCCTGGATGTGCCAAGTTCCATTTCTATGAGTTCAATGGGATCCAATGCAACTGAAACTGAACTTGGAGACCATTCAAGTCCATTCATGCATGCATGTGTTCCGTTCCAGCATTTACGAGTGTGCAAAGATTCTTATTGCACAATTTGCTCAACACGTCAGAGTCTGAAATCAGCTAAGCAGGAAAACCGAAAAGCTTCAAGGCTTTTCAATTCTAAGATTCATATCTCTTTCTATGACGATGCTAAAGGGTGGTTTAGAAGATGTTTCTCTTTTCTAAATCAACGTATTCCAGGAGTCATGAGCCCTCACACTAAGGGTGTGCAACAATGGAATAAATTCTTTGTCTTAGCTTGCTTGGTGGCGATTTTTATAGACCCTTTGTTTTTCTTCGTGTTATCTGCATGGAAGGAATATAAGTGCCTCGTTATCAGCAAGCCCCTGACCACAATAATGGTGTTCTTCAGAACAATAACTGATTTGATATACTTTTTGCACATGCTTCTCCAGTTTAGGTTGGCTTATGTTGCTCCTGAGTCTAGAGTAGTTGGTGCTGGAGAGATAGTGGACCAACCAAAGAAAATTGCTCTCCATTATCTCCAGGGAAAATTTCTCGTTGACTTGTTTATCGTTTTACCACTTCCTCAAATCATAATTTTGGCTATTTTACCACAATTTTTCGGATCATCTGGAGCAAATGACGCAAAGAATCTTCTACGCATGGCAGTTCTTTTCCAGTACATTCCCAGATTGTATAGATTTCTACCTCTGCTTGCTGGTCAGTCCCCATATGGATTTGTGCTTGAGTCAGCACGGACAAACTTCGTAATAAACCTCCTCACTTTCATGTTGGCTGGCCACGTTGTTGGGTCCTGCTGGTACCTCTTTGGCTTACAGAGGGTTAATCAATGTCTTCGAAATGCATGTCATAACTCTGATATCCCCAGTGAATTGTGCATGAAATTCATAGACTGTGGGTTTGGTGAGCAAGGATGGAAATGGGCGAACTGGATAGACAATGAGAATGCTACTACTTGTTTTAGTGAAGAGGGTTTTGCCTATGGAATCTATGTTAAAGCTGTCGATCTCACTACTGCAGATAGCGTAATAACTAAATATGTATACTCGTTGTTTTGGGGATTCCAGCAAATCAGCACTCTGGCGGGAAATCAAGTTCCAAGCTATTTTGTTTGGGAAGTCCTGTTTACCATGGCAATTATCGGACTTGGCCTCTTGCTTTTTGCTCTTCTAATTGGAAATATGCAGAATTTTCTTCAGTCTCTTGGACGGAGGAGATTTGAGATGTTATTGAGACGTCGTGATGTAGAATGGTGGATGAGCTATCGCCATCTACCAGAAGAACTGAGAAGGCAAGTAAGGCAAGCTGAACGGTATAACTGGTCTGCCACTAGAGGTGTAAATGAAGAAATGCTTTTGGAGAATCTGCCCGAAGACCTTCAAAGAGATATACGACGCCATCTCTTCAAATTTGTGAAAAAAGTTCGGATTTTTACCCTCCTAGAAGACCCGGTTTTGGATGCAATCTGTGAGAAACTAAAACAAAAGACATACATAAAAGGAAGCAAGATTTTCTATCCTGGAGGTCTAGTTGAGAAGATGGTTTTTATCGTGCGCGGAAGAGTAGAGAGCATTGGGGAGGATGGGATTGTTGTTTCGTTATGCGAAGGAGATGTTTGCGGCGAGGAACTTCTCACATGGTGTCTCGAGCATCCTTCAGTAAACCATGTCAGGATTCCTGGTCGGAAATTGTTGAGCAATAGGATGGTAAGGTGCTTAAGTAACGTTGAAGCATTTTCGCTGCGAGCAGCAGATCTTGAAGAGTTCACCAGCCGTTTTGCAAGATTCCTGCGGAATGCACGCGTGCAAGGAGCCATAAGGTATGAGTCACCGTATTGGAGAGGTCTTGCAGCAAAGAGAATTCAAGTAGCATGGAGATACAGGAAGAAGCGTCTAAGTCGTGCAAGCGTATCCCAGAACTCCTCTCCTCTGCATAGGACTTTTTGGTCGAACATCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

701

Amino Acids

80.87

Weight (kDa)

9.35

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 132 - 452 1.4e-17 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17700 AT3G17700
fragaria_vesca FvH4_5g18080 FvH4_5g18080 FvH4_5g18080 FvH4_6g44780
malus_domestica MD06G1236300.v1.1 MD09G1093300.v1.1 MD14G1243200.v1.1
prunus_persica Prupe.1G183800_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.5G240800_v2.0.a1 Prupe.5G240800_v2.0.a1 Prupe.5G240800_v2.0.a1
pyrus_communis pycom06g21110 pycom06g21140 pycom06g21190 pycom14g20450
rosa_chinensis RchiOBHm_Chr2g0161941 RchiOBHm_Chr4g0441651 RchiOBHm_Chr5g0083381 RchiOBHm_Chr6g0281101 RchiOBHm_Chr7g0177531
rosa_laevigata RLG00000005528 RLG00000015203 RLG00000015445 RLG00000021309 RLG00000023742 RLG00000034422 RLG00000036136
rosa_multiflora Rmu_co8432259.1_g000001 Rmu_co8481019.1_g000001 Rmu_sc0003410.1_g000009 Rmu_sc0004618.1_g000030 Rmu_sc0005144.1_g000007 Rmu_sc0008565.1_g000001 Rmu_sc0018381.1_g000003 Rmu_ssc0000330.1_g000004
rosa_roxburghii Rroxscaffold_1G00051700 Rroxscaffold_2G00088450 Rroxscaffold_2G00116130 Rroxscaffold_2G00126580 Rroxscaffold_3G00275780 Rroxscaffold_5G00338690 Rroxscaffold_5G00375080
rosa_rugosa Rorug02G0493400 Rorug04G0058900 Rorug04G0059000 Rorug06G0407100
rosa_samantha Rh2AG220400 Rh2AG282700 Rh2AG329100 Rh2AG559300 Rh2BG572700 Rh2CG542900 Rh2DG582500 Rh4AG135300 Rh4BG096100 Rh4DG026800 Rh4DG329100 Rh5CG271500 Rh6DG096900 Rh7AG006400 Rh7BG006300 Rh7CG006800 Rh7DG006400 Rh7DG278200
rosa_wichuraiana Rw0G014790 Rw1G017310 Rw7G000530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2086
AatII GACGTC 1 cut(s) 1381
Acc65I GGTACC 1 cut(s) 942
AccB1I GGYRCC 1 cut(s) 942
AccI GTMKAC 1 cut(s) 1185
AccII CGCG 2 cut(s) 1698, 1944
AciI CCGC 4 cut(s) 1226, 1698, 1761, 1933
AclI AACGTT 1 cut(s) 1866
AclWI GGATC 3 cut(s) 99, 112, 754
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 7 cut(s) 50, 401, 671, 1025, 1330, 1550, 2001
AcuI CTGAAG 3 cut(s) 511, 1322, 1782
AcyI GRCGYC 2 cut(s) 1378, 1537
AdeI CACNNNGTG 1 cut(s) 1782
AfaI GTAC 2 cut(s) 803, 944
AfiI CCNNNNNNNGG 1 cut(s) 1597
AflII CTTAAG 1 cut(s) 1858
AflIII ACRYGT 2 cut(s) 218, 1942
AjiI CACGTC 1 cut(s) 221
AjnI CCWGG 5 cut(s) 66, 355, 663, 1660, 1819
AjuI GAANNNNNNNTTGG 4 cut(s) 70, 102, 111, 143
AloI GAACNNNNNNTCC 2 cut(s) 1221, 1253
AluBI AGCT 7 cut(s) 239, 260, 416, 1142, 1245, 1404, 1448
AluI AGCT 7 cut(s) 239, 260, 416, 1142, 1245, 1404, 1448
Alw26I GTCTC 4 cut(s) 126, 1347, 1369, 1790
AlwI GGATC 3 cut(s) 99, 112, 754
AlwNI CAGNNNCTG 1 cut(s) 229
Ama87I CYCGRG 1 cut(s) 1787
AoxI GGCC 2 cut(s) 919, 1291
ApeKI GCWGC 3 cut(s) 1882, 1889, 1991
ApoI RAATTY 7 cut(s) 50, 401, 671, 1025, 1330, 1550, 2001
ArsI GACNNNNNNTTYG 4 cut(s) 973, 1005, 1025, 1057
Asp700I GAANNNNTTC 3 cut(s) 259, 401, 1518
Asp718I GGTACC 1 cut(s) 942
AspLEI GCGC 1 cut(s) 1698
AspS9I GGNCC 2 cut(s) 631, 933
AsuC2I CCSGG 1 cut(s) 1592
AsuHPI GGTGA 3 cut(s) 1058, 1900, 1962
AsuII TTCGAA 1 cut(s) 979
AvaI CYCGRG 1 cut(s) 1787
AvaII GGWCC 2 cut(s) 631, 933
BalI TGGCCA 1 cut(s) 921
BamHI GGATCC 1 cut(s) 104
BanI GGYRCC 1 cut(s) 942
BanII GRGCYC 1 cut(s) 371
BbsI GAAGAC 3 cut(s) 968, 1521, 1593
BbvI GCAGC 3 cut(s) 1869, 1901, 2003
BccI CCATC 6 cut(s) 1050, 1419, 1548, 1675, 1718, 1840
BceAI ACGGC 1 cut(s) 1899
BciT130I CCWGG 5 cut(s) 68, 357, 665, 1662, 1821
BciVI GTATCC 1 cut(s) 2062
BcnI CCSGG 1 cut(s) 1592
BcoDI GTCTC 4 cut(s) 126, 1347, 1369, 1790
BfaI CTAG 4 cut(s) 603, 1473, 1583, 1670
BfmI CTRYAG 1 cut(s) 1158
BfrI CTTAAG 1 cut(s) 1858
BfuI GTATCC 1 cut(s) 2062
BglII AGATCT 1 cut(s) 1894
BisI GCNGC 4 cut(s) 1762, 1883, 1890, 1992
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 4 cut(s) 1763, 1884, 1891, 1993
Bme1390I CCNGG 6 cut(s) 68, 357, 665, 1592, 1662, 1821
Bme18I GGWCC 2 cut(s) 631, 933
BmeT110I CYCGRG 1 cut(s) 1787
BmgBI CACGTC 1 cut(s) 221
BmgT120I GGNCC 2 cut(s) 631, 933
BmiI GGNNCC 4 cut(s) 106, 934, 944, 1954
BmrFI CCNGG 6 cut(s) 68, 357, 665, 1592, 1662, 1821
BmrI ACTGGG 1 cut(s) 1002
BmsI GCATC 3 cut(s) 295, 1591, 1801
BmuI ACTGGG 1 cut(s) 1002
BpiI GAAGAC 3 cut(s) 968, 1521, 1593
BpmI CTGGAG 5 cut(s) 554, 639, 647, 774, 1683
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 979
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 1 cut(s) 1592
BsaBI GATNNNNATC 1 cut(s) 287
BsaHI GRCGYC 2 cut(s) 1378, 1537
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 66, 664, 1272
BsaXI ACNNNNNCTCC 4 cut(s) 612, 642, 1656, 1686
Bsc4I CCNNNNNNNGG 1 cut(s) 1597
Bse1I ACTGG 5 cut(s) 571, 799, 1008, 1076, 1466
Bse8I GATNNNNATC 1 cut(s) 287
BseBI CCWGG 5 cut(s) 68, 357, 665, 1662, 1821
BseDI CCNNGG 3 cut(s) 66, 664, 1272
BseGI GGATG 7 cut(s) 76, 1061, 1404, 1606, 1729, 1792, 1851
BseJI GATNNNNATC 1 cut(s) 287
BseLI CCNNNNNNNGG 1 cut(s) 1597
BseMII CTCAG 3 cut(s) 53, 588, 1418
BseNI ACTGG 5 cut(s) 571, 799, 1008, 1076, 1466
BseRI GAGGAG 4 cut(s) 890, 1369, 2053, 2058
BseXI GCAGC 3 cut(s) 1869, 1901, 2003
Bsh1236I CGCG 2 cut(s) 1698, 1944
BshFI GGCC 2 cut(s) 921, 1293
BshNI GGYRCC 1 cut(s) 942
BsiHKCI CYCGRG 1 cut(s) 1787
BsiSI CCGG 1 cut(s) 1592
BslFI GGGAC 1 cut(s) 832
BslI CCNNNNNNNGG 1 cut(s) 1597
BsmAI GTCTC 4 cut(s) 126, 1347, 1369, 1790
BsmBI CGTCTC 1 cut(s) 1369
BsmFI GGGAC 1 cut(s) 832
BsmI GAATGC 2 cut(s) 1093, 1942
BsnI GGCC 2 cut(s) 921, 1293
Bso31I GGTCTC 1 cut(s) 126
BsoBI CYCGRG 1 cut(s) 1787
Bsp119I TTCGAA 1 cut(s) 979
Bsp1286I GDGCHC 1 cut(s) 371
Bsp143I GATC 4 cut(s) 104, 746, 1147, 1894
Bsp1720I GCTNAGC 1 cut(s) 240
Bsp19I CCATGG 1 cut(s) 1272
BspACI CCGC 4 cut(s) 1226, 1698, 1761, 1933
BspANI GGCC 2 cut(s) 921, 1293
BspCNI CTCAG 3 cut(s) 52, 589, 1419
BspFNI CGCG 2 cut(s) 1698, 1944
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 4 cut(s) 106, 934, 944, 1954
BspMAI CTGCAG 1 cut(s) 1162
BspPI GGATC 3 cut(s) 99, 112, 754
BspQI GCTCTTC 1 cut(s) 1314
BspT104I TTCGAA 1 cut(s) 979
BspT107I GGYRCC 1 cut(s) 942
BspTI CTTAAG 1 cut(s) 1858
BspTNI GGTCTC 1 cut(s) 126
BsrI ACTGG 5 cut(s) 571, 799, 1008, 1076, 1466
BssECI CCNNGG 3 cut(s) 66, 664, 1272
BssMI GATC 4 cut(s) 104, 746, 1147, 1894
BssNAI GTATAC 1 cut(s) 1186
BssNI GRCGYC 2 cut(s) 1378, 1537
BssT1I CCWWGG 1 cut(s) 1272
Bst1107I GTATAC 1 cut(s) 1186
Bst2UI CCWGG 5 cut(s) 68, 357, 665, 1662, 1821
Bst4CI ACNGT 3 cut(s) 1037, 1455, 1974
Bst6I CTCTTC 5 cut(s) 1104, 1314, 1550, 1696, 1896
BstACI GRCGYC 2 cut(s) 1378, 1537
BstAFI CTTAAG 1 cut(s) 1858
BstAPI GCANNNNNTGC 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 979
BstDEI CTNAG 8 cut(s) 39, 240, 279, 378, 412, 597, 1427, 2036
BstDSI CCRYGG 1 cut(s) 1272
BstF5I GGATG 7 cut(s) 76, 1061, 1404, 1606, 1729, 1792, 1851
BstFNI CGCG 2 cut(s) 1698, 1944
BstHHI GCGC 1 cut(s) 1698
BstKTI GATC 4 cut(s) 107, 749, 1150, 1897
BstMAI GTCTC 4 cut(s) 126, 1347, 1369, 1790
BstMBI GATC 4 cut(s) 104, 746, 1147, 1894
BstMWI GCNNNNNNNGC 5 cut(s) 10, 210, 590, 764, 1441
BstNI CCWGG 5 cut(s) 68, 357, 665, 1662, 1821
BstNSI RCATGY 4 cut(s) 158, 162, 565, 990
BstSCI CCNGG 6 cut(s) 66, 355, 663, 1590, 1660, 1819
BstSFI CTRYAG 1 cut(s) 1158
BstUI CGCG 2 cut(s) 1698, 1944
BstV1I GCAGC 3 cut(s) 1869, 1901, 2003
BstV2I GAAGAC 3 cut(s) 968, 1521, 1593
BstX2I RGATCY 2 cut(s) 104, 1894
BstXI CCANNNNNNTGG 3 cut(s) 67, 517, 929
BstYI RGATCY 2 cut(s) 104, 1894
BstZ17I GTATAC 1 cut(s) 1186
BsuI GTATCC 1 cut(s) 2062
BsuRI GGCC 2 cut(s) 921, 1293
BtgI CCRYGG 1 cut(s) 1272
BtrI CACGTC 1 cut(s) 221
BtsCI GGATG 7 cut(s) 76, 1061, 1404, 1606, 1729, 1792, 1851
BtsIMutI CAGTG 1 cut(s) 1015
CaiI CAGNNNCTG 1 cut(s) 229
CciI TCATGA 1 cut(s) 363
CfoI GCGC 1 cut(s) 1698
Cfr13I GGNCC 2 cut(s) 631, 933
CseI GACGC 3 cut(s) 773, 1545, 2021
Csp6I GTAC 2 cut(s) 802, 943
CviQI GTAC 2 cut(s) 802, 943
DdeI CTNAG 8 cut(s) 39, 240, 279, 378, 412, 597, 1427, 2036
DpnI GATC 4 cut(s) 106, 748, 1149, 1896
DpnII GATC 4 cut(s) 104, 746, 1147, 1894
DraIII CACNNNGTG 1 cut(s) 1782
DrdI GACNNNNNNGTC 1 cut(s) 2086
DseDI GACNNNNNNGTC 1 cut(s) 2086
EaeI YGGCCR 1 cut(s) 919
Eam1104I CTCTTC 5 cut(s) 1104, 1314, 1550, 1696, 1896
EarI CTCTTC 5 cut(s) 1104, 1314, 1550, 1696, 1896
Eco130I CCWWGG 1 cut(s) 1272
Eco24I GRGCYC 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 126
Eco32I GATATC 1 cut(s) 1003
Eco47I GGWCC 2 cut(s) 631, 933
Eco57I CTGAAG 3 cut(s) 511, 1322, 1782
Eco88I CYCGRG 1 cut(s) 1787
EcoO109I RGGNCCY 1 cut(s) 933
EcoRI GAATTC 1 cut(s) 2001
EcoRII CCWGG 5 cut(s) 66, 355, 663, 1660, 1819
EcoRV GATATC 1 cut(s) 1003
EcoT14I CCWWGG 1 cut(s) 1272
EcoT22I ATGCAT 3 cut(s) 156, 160, 988
EcoT38I GRGCYC 1 cut(s) 371
ErhI CCWWGG 1 cut(s) 1272
Esp3I CGTCTC 1 cut(s) 1369
FalI AAGNNNNNCTT 2 cut(s) 1479, 1511
FaqI GGGAC 1 cut(s) 832
FauI CCCGC 1 cut(s) 1219
FauNDI CATATG 1 cut(s) 853
FblI GTMKAC 1 cut(s) 1185
Fnu4HI GCNGC 4 cut(s) 1762, 1883, 1890, 1992
FokI GGATG 7 cut(s) 83, 1068, 1411, 1613, 1736, 1779, 1858
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 4 cut(s) 1762, 1883, 1890, 1992
FspBI CTAG 4 cut(s) 603, 1473, 1583, 1670
GlaI GCGC 1 cut(s) 1697
GluI GCNGC 4 cut(s) 1762, 1883, 1890, 1992
GsuI CTGGAG 5 cut(s) 554, 639, 647, 774, 1683
HaeIII GGCC 2 cut(s) 921, 1293
HapII CCGG 1 cut(s) 1592
HgaI GACGC 3 cut(s) 773, 1545, 2021
HhaI GCGC 1 cut(s) 1698
Hin1I GRCGYC 2 cut(s) 1378, 1537
Hin6I GCGC 1 cut(s) 1696
HinP1I GCGC 1 cut(s) 1696
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HindIII AAGCTT 1 cut(s) 258
HpaII CCGG 1 cut(s) 1592
HphI GGTGA 3 cut(s) 1058, 1900, 1962
Hpy166II GTNNAC 6 cut(s) 631, 682, 1186, 1269, 1804, 1908
Hpy188I TCNGA 9 cut(s) 225, 231, 530, 746, 1000, 1286, 1569, 1827, 2099
Hpy188III TCNNGA 9 cut(s) 46, 364, 596, 603, 753, 1382, 1787, 1813, 1898
Hpy8I GTNNAC 6 cut(s) 631, 682, 1186, 1269, 1804, 1908
Hpy99I CGWCG 2 cut(s) 1383, 1539
HpyAV CCTTC 5 cut(s) 466, 1425, 1529, 1742, 1806
HpyCH4III ACNGT 3 cut(s) 1037, 1455, 1974
HpyCH4IV ACGT 5 cut(s) 220, 349, 924, 1378, 1866
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 210, 590, 764, 1441
HpyF3I CTNAG 8 cut(s) 39, 240, 279, 378, 412, 597, 1427, 2036
HpySE526I ACGT 5 cut(s) 220, 349, 924, 1378, 1866
Hsp92I GRCGYC 2 cut(s) 1378, 1537
HspAI GCGC 1 cut(s) 1696
KpnI GGTACC 1 cut(s) 946
Kzo9I GATC 4 cut(s) 104, 746, 1147, 1894
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 3 cut(s) 598, 755, 1952
Lsp1109I GCAGC 3 cut(s) 1869, 1901, 2003
LweI GCATC 3 cut(s) 295, 1591, 1801
MaeI CTAG 4 cut(s) 603, 1473, 1583, 1670
MaeII ACGT 5 cut(s) 220, 349, 924, 1378, 1866
MaeIII GTNAC 2 cut(s) 1862, 1968
MalI GATC 4 cut(s) 106, 748, 1149, 1896
MboI GATC 4 cut(s) 104, 746, 1147, 1894
MflI RGATCY 2 cut(s) 104, 1894
MhlI GDGCHC 1 cut(s) 371
MlsI TGGCCA 1 cut(s) 921
MluI ACGCGT 1 cut(s) 1942
MluNI TGGCCA 1 cut(s) 921
MlyI GAGTC 5 cut(s) 235, 369, 608, 878, 1976
MmeI TCCRAC 1 cut(s) 1805
Mox20I TGGCCA 1 cut(s) 921
Mph1103I ATGCAT 3 cut(s) 156, 160, 988
MroXI GAANNNNTTC 3 cut(s) 259, 401, 1518
MscI TGGCCA 1 cut(s) 921
MseI TTAA 3 cut(s) 966, 1137, 1859
MslI CAYNNNNRTG 4 cut(s) 89, 159, 515, 908
Msp20I TGGCCA 1 cut(s) 921
MspCI CTTAAG 1 cut(s) 1858
MspI CCGG 1 cut(s) 1592
MspR9I CCNGG 6 cut(s) 68, 357, 665, 1592, 1662, 1821
Mva1269I GAATGC 2 cut(s) 1093, 1942
MvaI CCWGG 5 cut(s) 68, 357, 665, 1662, 1821
MvnI CGCG 2 cut(s) 1698, 1944
MwoI GCNNNNNNNGC 5 cut(s) 10, 210, 590, 764, 1441
NciI CCSGG 1 cut(s) 1592
NcoI CCATGG 1 cut(s) 1272
NdeI CATATG 1 cut(s) 853
NdeII GATC 4 cut(s) 104, 746, 1147, 1894
NlaIV GGNNCC 4 cut(s) 106, 934, 944, 1954
NmuCI GTSAC 1 cut(s) 1968
NsiI ATGCAT 3 cut(s) 156, 160, 988
NspI RCATGY 4 cut(s) 158, 162, 565, 990
NspV TTCGAA 1 cut(s) 979
PaeI GCATGC 1 cut(s) 158
PaeR7I CTCGAG 1 cut(s) 1787
PagI TCATGA 1 cut(s) 363
PciSI GCTCTTC 1 cut(s) 1314
PctI GAATGC 2 cut(s) 1093, 1942
PdmI GAANNNNTTC 3 cut(s) 259, 401, 1518
PfoI TCCNGGA 2 cut(s) 355, 1660
PkrI GCNGC 4 cut(s) 1763, 1884, 1891, 1993
PleI GAGTC 5 cut(s) 234, 368, 607, 877, 1975
PpsI GAGTC 5 cut(s) 234, 368, 607, 877, 1975
PpuMI RGGWCCY 1 cut(s) 933
Psp1406I AACGTT 1 cut(s) 1866
Psp5II RGGWCCY 1 cut(s) 933
Psp6I CCWGG 5 cut(s) 66, 355, 663, 1660, 1819
PspGI CCWGG 5 cut(s) 66, 355, 663, 1660, 1819
PspN4I GGNNCC 4 cut(s) 106, 934, 944, 1954
PspPI GGNCC 2 cut(s) 631, 933
PspPPI RGGWCCY 1 cut(s) 933
PstI CTGCAG 1 cut(s) 1162
PstNI CAGNNNCTG 1 cut(s) 229
PsuI RGATCY 2 cut(s) 104, 1894
RsaI GTAC 2 cut(s) 803, 944
RsaNI GTAC 2 cut(s) 802, 943
RseI CAYNNNNRTG 4 cut(s) 89, 159, 515, 908
SapI GCTCTTC 1 cut(s) 1314
SaqAI TTAA 3 cut(s) 966, 1137, 1859
SatI GCNGC 4 cut(s) 1762, 1883, 1890, 1992
Sau3AI GATC 4 cut(s) 104, 746, 1147, 1894
Sau96I GGNCC 2 cut(s) 631, 933
SchI GAGTC 5 cut(s) 235, 369, 608, 878, 1976
ScrFI CCNGG 6 cut(s) 68, 357, 665, 1592, 1662, 1821
SduI GDGCHC 1 cut(s) 371
SfaNI GCATC 3 cut(s) 295, 1591, 1801
SfcI CTRYAG 1 cut(s) 1158
Sfr274I CTCGAG 1 cut(s) 1787
SfuI TTCGAA 1 cut(s) 979
SinI GGWCC 2 cut(s) 631, 933
SlaI CTCGAG 1 cut(s) 1787
SmiMI CAYNNNNRTG 4 cut(s) 89, 159, 515, 908
SmlI CTYRAG 3 cut(s) 866, 1787, 1858
SmoI CTYRAG 3 cut(s) 866, 1787, 1858
SphI GCATGC 1 cut(s) 158
SsiI CCGC 4 cut(s) 1226, 1698, 1761, 1933
SspMI CTAG 4 cut(s) 603, 1473, 1583, 1670
StyD4I CCNGG 6 cut(s) 66, 355, 663, 1590, 1660, 1819
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 3 cut(s) 1037, 1455, 1974
TaiI ACGT 5 cut(s) 223, 352, 927, 1381, 1869
TaqI TCGA 4 cut(s) 979, 1146, 1788, 2090
TatI WGTACW 1 cut(s) 801
TauI GCSGC 1 cut(s) 1764
Tru1I TTAA 3 cut(s) 966, 1137, 1859
Tru9I TTAA 3 cut(s) 966, 1137, 1859
TscAI CASTG 1 cut(s) 1015
TseFI GTSAC 1 cut(s) 1968
TseI GCWGC 3 cut(s) 1882, 1889, 1991
Tsp45I GTSAC 1 cut(s) 1968
TspDTI ATGAA 6 cut(s) 139, 275, 898, 1018, 1037, 1500
TspGWI ACGGA 3 cut(s) 156, 892, 1366
TspRI CASTG 1 cut(s) 1015
Vha464I CTTAAG 1 cut(s) 1858
VpaK11BI GGWCC 2 cut(s) 631, 933
XapI RAATTY 7 cut(s) 50, 401, 671, 1025, 1330, 1550, 2001
XbaI TCTAGA 1 cut(s) 602
XceI RCATGY 4 cut(s) 158, 162, 565, 990
XhoI CTCGAG 1 cut(s) 1787
XmiI GTMKAC 1 cut(s) 1185
XmnI GAANNNNTTC 3 cut(s) 259, 401, 1518
XspI CTAG 4 cut(s) 603, 1473, 1583, 1670
ZraI GACGTC 1 cut(s) 1379
Zsp2I ATGCAT 3 cut(s) 156, 160, 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.