RLG00000021309

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000678
Physical Location & Seq
Forward (+)
74038153 .. 74045078
6926 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021309

Sequence Viewer

Length: 2265 bp
ATGCGCCGGTTCTGGAGTCAATACTCGGATAACAGTTCAGCTTTGACTAGGCCTCCTAGAAGACAAATGGGTAAAATAAGAAACGATGAATTACCAATGCTACCTTCACAATTACCATCCAGTTACGAGAATACATTTAGTTCCATTTCTGTCAATTCAATGGAATCCAGTGGAACTGGAATTGGCCGTGTAGACCATACAGGTTCCTTACAGATTGATAGCAAAAATCCTTTCACACCTGCATGTGTTCCATTCCAGCAGTTTACAGATTGCAATGATCCTCACTGCACATTTTGCAAACCACGTCATAGTTTGAAATCAGTTCAACACAAAAACTACATAGCTTCAAGTTTGTTTGATCATAAGCTCCATAATATTCTGTATGGTGATGCTAAAGGATGGTTTAAAAGAACCTGTTCTTTTCTGTATCCATATGTTCTCGGAGTTGTGAACCCTGATGCTAAAGCTGTACGAGGATGGAACAAGTTCTTTGTCATATCTTGCATGGTGGCAGTTTTTGTAGACCCGCTATTTTTCTTCTTGTTATCTGCACGAGAGGATTATAAGTGCATAACTATTAACTGGGCCATGACCATAATATTAGTGTTTTTCCGGACACTGACGGACTTCATTTACCTATTGCACATGCTTCTTCAGTTTAGGTTGGCTTACGTGGCTCCTGGGTCTAGAGTGGTTGGTACCGGAGAGATAGTAGACCAACCATATAAAATTGCTCTCAATTATCTCCATGGAAACTTTTTAGTCGACTTAATTATTGTTTTGCCACTTCCTCAGATCATAATGTTGTCGGTTGTTCCTTTGGGATCGTTTCGAGCAAATCAAACAAAGAATCTTCTCCGCCTGGCAGTATTTTTTCAATACTTTCCCAGGTTGTATAGGGTTCTGCCCCTGCTTGCTGGTCAATCTCCAAATGGATTCTTTGTATTTGAGTCAGCTTGGACAAATTTTTTTATAAACACTCTCACTTTCTTGTTGGTTGGCCATGTTATTGGGTCATGCTGGTACCTTCTTGGTTTACAGAGGGTTAACCAATGTCTTCGAGATGCCTGCCATGACTCTAATCTGCAGTCATGCGGGAGTTTAATAGATTGTTGCAATGCTCAATCTATTCCAGAGTCAACTAGATGGAGAGAAAATGAGAAGGCTGCTGGTTGTTTTAATCCGGAAGGAGATTTCTCCTATGGAATATATACTCCAGTTGTCAATCTCACTACAAGAGATAGCATGACTAAATATTTATACTCACTGTTTTGGGGATTCCAGCAAATCAGCACTTTGGCAGGAAATCAAACTCCAAGCTATTTTAATGGGGAAATTGTTTTTACCATGGTAATTATTGGACTGGGACTCTTCTTCTTTGCTCTTCTAATTGGAAACATGCAGAATTTTCTTCAGTCTCTTGGACGGAGGAGGTTTGATATGTTATTGCGGAGTCGTGATGTTGAGCAGTGGATGAGTTATAGGAACTTACCAGAAGAGCTTAGAAGGCAAGTAAGAAATGCTGAACGGTATAATTGGTCTGCCACACAAGGTGTAAATGAAGAACTGATTCTGGAAAATTTGCCCGAACACCTTCAAAGAAATATAAGACGCCATCTCTTCGAATTTGTGAAGAAAGTTCGGATTTTTGCTCTGATGGACGATCCTATCTTAGATGCAATATGTGAGAAACTAAAACAAAAAACATACATTGAAGGAAATGAAATTTTGTATCCTGGAGGTATGGTTGAGAATATGGTTTTTATTGTGCGTGGAAAGATGGAGAGCATTGGGGCGGATGGGACTGTTGATGACACATTAAAGGAAGGTGATGTTTGTGGTGAGGAACTTCTCAGATGGTGTCTTGAGGCTTCTGTAAACACCGATGGGAAAAATGTCAGGATCACTGGTCGGAGATTGCTCAGCAACAGATTGGTAAGGTGCTTAACTAATGTGGAAGCATTTCAACTCCGAGCTGCAGACCTCGAACAGTTCACTGCACGTTTTGCAAGATACTTGCGGAATCCACGTGTACAAGGAGCCATAAGGTATGAATCTCCGTACTGGAGAGGGCTTGCAGCAAAGCGAATTCAAGTAGCTTGGAGGTACAGGCAAAAGCGTCTAAATCGCACAAGCCCACAGAGTTGCCCCCGAAACCCTTACTCAGATCAAAGTGCAGCGGACTTCTTGAGATCAACTTATCGCCCACATAGTTGGGGACTTGGAAATGATGCTCGGGAACGGGAACACTGGGGCCTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

755

Amino Acids

86.93

Weight (kDa)

9.16

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 162 - 477 2.6e-19 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17700 AT3G17700
fragaria_vesca FvH4_5g18080 FvH4_5g18080 FvH4_5g18080 FvH4_6g44780
malus_domestica MD06G1236300.v1.1 MD09G1093300.v1.1 MD14G1243200.v1.1
prunus_persica Prupe.1G183800_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.5G240800_v2.0.a1 Prupe.5G240800_v2.0.a1 Prupe.5G240800_v2.0.a1
pyrus_communis pycom06g21110 pycom06g21140 pycom06g21190 pycom14g20450
rosa_chinensis RchiOBHm_Chr2g0161941 RchiOBHm_Chr4g0441651 RchiOBHm_Chr5g0083381 RchiOBHm_Chr6g0281101 RchiOBHm_Chr7g0177531
rosa_laevigata RLG00000005528 RLG00000015203 RLG00000015445 RLG00000021309 RLG00000023742 RLG00000034422 RLG00000036136
rosa_multiflora Rmu_co8432259.1_g000001 Rmu_co8481019.1_g000001 Rmu_sc0003410.1_g000009 Rmu_sc0004618.1_g000030 Rmu_sc0005144.1_g000007 Rmu_sc0008565.1_g000001 Rmu_sc0018381.1_g000003 Rmu_ssc0000330.1_g000004
rosa_roxburghii Rroxscaffold_1G00051700 Rroxscaffold_2G00088450 Rroxscaffold_2G00116130 Rroxscaffold_2G00126580 Rroxscaffold_3G00275780 Rroxscaffold_5G00338690 Rroxscaffold_5G00375080
rosa_rugosa Rorug02G0493400 Rorug04G0058900 Rorug04G0059000 Rorug06G0407100
rosa_samantha Rh2AG220400 Rh2AG282700 Rh2AG329100 Rh2AG559300 Rh2BG572700 Rh2CG542900 Rh2DG582500 Rh4AG135300 Rh4BG096100 Rh4DG026800 Rh4DG329100 Rh5CG271500 Rh6DG096900 Rh7AG006400 Rh7BG006300 Rh7CG006800 Rh7DG006400 Rh7DG278200
rosa_wichuraiana Rw0G014790 Rw1G017310 Rw7G000530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 564, 974
AarI CACCTGC 1 cut(s) 247
Acc36I ACCTGC 1 cut(s) 247
Acc65I GGTACC 2 cut(s) 698, 1023
AccB1I GGYRCC 2 cut(s) 698, 1023
AccI GTMKAC 4 cut(s) 192, 522, 714, 765
AccIII TCCGGA 2 cut(s) 612, 1183
AciI CCGC 7 cut(s) 527, 859, 1095, 1450, 1796, 2020, 2180
AclWI GGATC 4 cut(s) 272, 832, 1658, 1910
AcoI YGGCCR 2 cut(s) 184, 1000
AcsI RAATTY 6 cut(s) 964, 1405, 1579, 1625, 1725, 2088
AcuI CTGAAG 2 cut(s) 638, 1397
AcvI CACGTG 1 cut(s) 2030
AcyI GRCGYC 1 cut(s) 1612
AdeI CACNNNGTG 1 cut(s) 1553
AfaI GTAC 6 cut(s) 471, 700, 1025, 2034, 2063, 2108
AflIII ACRYGT 1 cut(s) 2029
AgsI TTSAA 9 cut(s) 159, 316, 326, 348, 878, 1598, 1715, 1967, 2093
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 4 cut(s) 679, 861, 887, 1735
AjuI GAANNNNNNNTTGG 2 cut(s) 88, 120
AluBI AGCT 9 cut(s) 41, 344, 367, 467, 956, 1320, 1501, 1976, 2099
AluI AGCT 9 cut(s) 41, 344, 367, 467, 956, 1320, 1501, 1976, 2099
Alw26I GTCTC 1 cut(s) 1422
AlwI GGATC 4 cut(s) 272, 832, 1658, 1910
Ama87I CYCGRG 1 cut(s) 2235
Aor13HI TCCGGA 2 cut(s) 612, 1183
AoxI GGCC 5 cut(s) 50, 184, 585, 1000, 2254
ApeKI GCWGC 4 cut(s) 1166, 1976, 2078, 2177
ApoI RAATTY 6 cut(s) 964, 1405, 1579, 1625, 1725, 2088
Asp700I GAANNNNTTC 5 cut(s) 415, 485, 1569, 1593, 1962
Asp718I GGTACC 2 cut(s) 698, 1023
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 2 cut(s) 585, 2254
AsuHPI GGTGA 3 cut(s) 398, 1841, 1853
AsuII TTCGAA 1 cut(s) 1623
AvaI CYCGRG 1 cut(s) 2235
BalI TGGCCA 1 cut(s) 1002
BanI GGYRCC 2 cut(s) 698, 1023
BauI CACGAG 1 cut(s) 552
BbrPI CACGTG 1 cut(s) 2030
BbsI GAAGAC 2 cut(s) 67, 1049
BbvI GCAGC 4 cut(s) 1153, 1963, 2090, 2189
BceAI ACGGC 1 cut(s) 171
BcgI CGANNNNNNTGC 2 cut(s) 1050, 1084
BciT130I CCWGG 4 cut(s) 681, 863, 889, 1737
BciVI GTATCC 2 cut(s) 438, 1743
BclI TGATCA 1 cut(s) 358
BcoDI GTCTC 1 cut(s) 1422
BfaI CTAG 4 cut(s) 48, 57, 687, 1143
BfmI CTRYAG 2 cut(s) 1085, 1977
BfuAI ACCTGC 1 cut(s) 247
BfuI GTATCC 2 cut(s) 438, 1743
BisI GCNGC 4 cut(s) 1167, 1977, 2079, 2178
BlpI GCTNAGC 1 cut(s) 1922
BlsI GCNGC 4 cut(s) 1168, 1978, 2080, 2179
Bme1390I CCNGG 4 cut(s) 681, 863, 889, 1737
BmeT110I CYCGRG 1 cut(s) 2235
BmgBI CACGTC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 585, 2254
BmiI GGNNCC 6 cut(s) 205, 678, 700, 1025, 2041, 2255
BmrFI CCNGG 4 cut(s) 681, 863, 889, 1737
BmrI ACTGGG 3 cut(s) 592, 1373, 2260
BmsI GCATC 5 cut(s) 379, 448, 1054, 1666, 2221
BmuI ACTGGG 3 cut(s) 592, 1373, 2260
BpiI GAAGAC 2 cut(s) 67, 1049
BpmI CTGGAG 4 cut(s) 34, 1200, 1758, 2086
Bpu1102I GCTNAGC 1 cut(s) 1922
Bpu14I TTCGAA 1 cut(s) 1623
BpuEI CTTGAG 2 cut(s) 1886, 2209
BsaAI YACGTR 2 cut(s) 673, 2030
BsaHI GRCGYC 1 cut(s) 1612
BsaJI CCNNGG 4 cut(s) 680, 748, 887, 1347
BsaWI WCCGGW 3 cut(s) 612, 701, 1183
BsaXI ACNNNNNCTCC 6 cut(s) 37, 67, 696, 726, 1731, 1761
Bse118I RCCGGY 1 cut(s) 6
Bse1I ACTGG 9 cut(s) 120, 168, 181, 587, 1217, 1368, 1912, 2069, 2255
Bse3DI GCAATG 2 cut(s) 280, 1123
BseAI TCCGGA 2 cut(s) 612, 1183
BseBI CCWGG 4 cut(s) 681, 863, 889, 1737
BseDI CCNNGG 4 cut(s) 680, 748, 887, 1347
BseGI GGATG 5 cut(s) 116, 404, 482, 1479, 1804
BseMI GCAATG 2 cut(s) 280, 1123
BseMII CTCAG 4 cut(s) 806, 1867, 1936, 2178
BseNI ACTGG 9 cut(s) 120, 168, 181, 587, 1217, 1368, 1912, 2069, 2255
BseRI GAGGAG 1 cut(s) 1444
BseXI GCAGC 4 cut(s) 1153, 1963, 2090, 2189
BsgI GTGCAG 4 cut(s) 271, 534, 1983, 2196
BshFI GGCC 5 cut(s) 52, 186, 587, 1002, 2256
BshNI GGYRCC 2 cut(s) 698, 1023
BsiHKCI CYCGRG 1 cut(s) 2235
BsiSI CCGG 4 cut(s) 7, 613, 702, 1184
BslFI GGGAC 3 cut(s) 1380, 1816, 2232
BsmAI GTCTC 1 cut(s) 1422
BsmFI GGGAC 3 cut(s) 1380, 1816, 2232
BsnI GGCC 5 cut(s) 52, 186, 587, 1002, 2256
BsoBI CYCGRG 1 cut(s) 2235
Bsp119I TTCGAA 1 cut(s) 1623
Bsp13I TCCGGA 2 cut(s) 612, 1183
Bsp1407I TGTACA 1 cut(s) 2032
Bsp143I GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
Bsp1720I GCTNAGC 1 cut(s) 1922
Bsp19I CCATGG 2 cut(s) 748, 1347
BspACI CCGC 7 cut(s) 527, 859, 1095, 1450, 1796, 2020, 2180
BspANI GGCC 5 cut(s) 52, 186, 587, 1002, 2256
BspCNI CTCAG 4 cut(s) 805, 1866, 1935, 2177
BspEI TCCGGA 2 cut(s) 612, 1183
BspLI GGNNCC 6 cut(s) 205, 678, 700, 1025, 2041, 2255
BspMAI CTGCAG 2 cut(s) 1089, 1981
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 4 cut(s) 272, 832, 1658, 1910
BspQI GCTCTTC 2 cut(s) 1389, 1491
BspT104I TTCGAA 1 cut(s) 1623
BspT107I GGYRCC 2 cut(s) 698, 1023
BsrDI GCAATG 2 cut(s) 280, 1123
BsrFI RCCGGY 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 2032
BsrI ACTGG 9 cut(s) 120, 168, 181, 587, 1217, 1368, 1912, 2069, 2255
BssAI RCCGGY 1 cut(s) 6
BssECI CCNNGG 4 cut(s) 680, 748, 887, 1347
BssMI GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
BssNI GRCGYC 1 cut(s) 1612
BssSI CACGAG 1 cut(s) 552
BssT1I CCWWGG 2 cut(s) 748, 1347
Bst2BI CACGAG 1 cut(s) 552
Bst2UI CCWGG 4 cut(s) 681, 863, 889, 1737
Bst4CI ACNGT 5 cut(s) 35, 1269, 1530, 1807, 1992
Bst6I CTCTTC 4 cut(s) 1376, 1389, 1491, 1625
BstACI GRCGYC 1 cut(s) 1612
BstAPI GCANNNNNTGC 2 cut(s) 294, 2006
BstAUI TGTACA 1 cut(s) 2032
BstBAI YACGTR 2 cut(s) 673, 2030
BstBI TTCGAA 1 cut(s) 1623
BstC8I GCNNGC 3 cut(s) 915, 1069, 2076
BstDEI CTNAG 6 cut(s) 792, 1502, 1672, 1853, 1922, 2164
BstDSI CCRYGG 2 cut(s) 748, 1347
BstF5I GGATG 5 cut(s) 116, 404, 482, 1479, 1804
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 8 cut(s) 280, 361, 798, 827, 1666, 1905, 2170, 2195
BstMAI GTCTC 1 cut(s) 1422
BstMBI GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
BstMWI GCNNNNNNNGC 4 cut(s) 294, 674, 1507, 2006
BstNI CCWGG 4 cut(s) 681, 863, 889, 1737
BstNSI RCATGY 3 cut(s) 246, 649, 1402
BstSCI CCNGG 4 cut(s) 679, 861, 887, 1735
BstSFI CTRYAG 2 cut(s) 1085, 1977
BstV1I GCAGC 4 cut(s) 1153, 1963, 2090, 2189
BstV2I GAAGAC 2 cut(s) 67, 1049
BstXI CCANNNNNNTGG 2 cut(s) 1010, 2214
BsuI GTATCC 2 cut(s) 438, 1743
BsuRI GGCC 5 cut(s) 52, 186, 587, 1002, 2256
BtgI CCRYGG 2 cut(s) 748, 1347
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 5 cut(s) 116, 404, 482, 1479, 1804
BtsI GCAGTG 3 cut(s) 283, 1475, 1995
BtsIMutI CAGTG 8 cut(s) 175, 283, 617, 1265, 1475, 1905, 1995, 2248
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 915, 1069, 2076
CfoI GCGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 6
Cfr13I GGNCC 2 cut(s) 585, 2254
CseI GACGC 2 cut(s) 1620, 2108
Csp6I GTAC 6 cut(s) 470, 699, 1024, 2033, 2062, 2107
CviQI GTAC 6 cut(s) 470, 699, 1024, 2033, 2062, 2107
DdeI CTNAG 6 cut(s) 792, 1502, 1672, 1853, 1922, 2164
DpnI GATC 8 cut(s) 279, 360, 797, 826, 1665, 1904, 2169, 2194
DpnII GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
DraI TTTAAA 1 cut(s) 406
DraIII CACNNNGTG 1 cut(s) 1553
EaeI YGGCCR 2 cut(s) 184, 1000
Eam1104I CTCTTC 4 cut(s) 1376, 1389, 1491, 1625
EarI CTCTTC 4 cut(s) 1376, 1389, 1491, 1625
EciI GGCGGA 2 cut(s) 848, 1811
Eco130I CCWWGG 2 cut(s) 748, 1347
Eco147I AGGCCT 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 638, 1397
Eco72I CACGTG 1 cut(s) 2030
Eco88I CYCGRG 1 cut(s) 2235
EcoO109I RGGNCCY 1 cut(s) 2254
EcoRI GAATTC 1 cut(s) 2088
EcoRII CCWGG 4 cut(s) 679, 861, 887, 1735
EcoT14I CCWWGG 2 cut(s) 748, 1347
ErhI CCWWGG 2 cut(s) 748, 1347
FaqI GGGAC 3 cut(s) 1380, 1816, 2232
FauI CCCGC 2 cut(s) 534, 1088
FauNDI CATATG 1 cut(s) 433
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 4 cut(s) 192, 522, 714, 765
Fnu4HI GCNGC 4 cut(s) 1167, 1977, 2079, 2178
FokI GGATG 5 cut(s) 103, 411, 489, 1486, 1811
Fsp4HI GCNGC 4 cut(s) 1167, 1977, 2079, 2178
FspBI CTAG 4 cut(s) 48, 57, 687, 1143
GlaI GCGC 1 cut(s) 5
GluI GCNGC 4 cut(s) 1167, 1977, 2079, 2178
GsuI CTGGAG 4 cut(s) 34, 1200, 1758, 2086
HaeIII GGCC 5 cut(s) 52, 186, 587, 1002, 2256
HapII CCGG 4 cut(s) 7, 613, 702, 1184
HgaI GACGC 2 cut(s) 1620, 2108
HhaI GCGC 1 cut(s) 6
Hin1I GRCGYC 1 cut(s) 1612
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 3 cut(s) 766, 1048, 1140
HindII GTYRAC 3 cut(s) 766, 1048, 1140
HpaI GTTAAC 1 cut(s) 1048
HpaII CCGG 4 cut(s) 7, 613, 702, 1184
HphI GGTGA 3 cut(s) 398, 1841, 1853
HpyAV CCTTC 8 cut(s) 114, 1037, 1156, 1181, 1500, 1604, 1709, 1820
HpyCH4III ACNGT 5 cut(s) 35, 1269, 1530, 1807, 1992
HpyCH4IV ACGT 4 cut(s) 304, 672, 2002, 2029
HpyF10VI GCNNNNNNNGC 4 cut(s) 294, 674, 1507, 2006
HpyF3I CTNAG 6 cut(s) 792, 1502, 1672, 1853, 1922, 2164
HpySE526I ACGT 4 cut(s) 304, 672, 2002, 2029
Hsp92I GRCGYC 1 cut(s) 1612
HspAI GCGC 1 cut(s) 4
Kpn2I TCCGGA 2 cut(s) 612, 1183
KpnI GGTACC 2 cut(s) 702, 1027
Ksp22I TGATCA 1 cut(s) 358
KspAI GTTAAC 1 cut(s) 1048
Kzo9I GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
LguI GCTCTTC 2 cut(s) 1389, 1491
LmnI GCTCC 3 cut(s) 372, 682, 2039
Lsp1109I GCAGC 4 cut(s) 1153, 1963, 2090, 2189
LweI GCATC 5 cut(s) 379, 448, 1054, 1666, 2221
MaeI CTAG 4 cut(s) 48, 57, 687, 1143
MaeII ACGT 4 cut(s) 304, 672, 2002, 2029
MaeIII GTNAC 1 cut(s) 122
MalI GATC 8 cut(s) 279, 360, 797, 826, 1665, 1904, 2169, 2194
MboI GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
MlsI TGGCCA 1 cut(s) 1002
MluNI TGGCCA 1 cut(s) 1002
MlyI GAGTC 6 cut(s) 25, 959, 1070, 1145, 1362, 1462
MmeI TCCRAC 1 cut(s) 1892
Mox20I TGGCCA 1 cut(s) 1002
MroI TCCGGA 2 cut(s) 612, 1183
MroXI GAANNNNTTC 5 cut(s) 415, 485, 1569, 1593, 1962
MscI TGGCCA 1 cut(s) 1002
MseI TTAA 9 cut(s) 405, 579, 770, 1047, 1103, 1179, 1326, 1820, 1946
MslI CAYNNNNRTG 1 cut(s) 241
Msp20I TGGCCA 1 cut(s) 1002
MspA1I CMGCKG 1 cut(s) 2180
MspI CCGG 4 cut(s) 7, 613, 702, 1184
MspR9I CCNGG 4 cut(s) 681, 863, 889, 1737
MvaI CCWGG 4 cut(s) 681, 863, 889, 1737
MwoI GCNNNNNNNGC 4 cut(s) 294, 674, 1507, 2006
NcoI CCATGG 2 cut(s) 748, 1347
NdeI CATATG 1 cut(s) 433
NdeII GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
NlaIV GGNNCC 6 cut(s) 205, 678, 700, 1025, 2041, 2255
NspI RCATGY 3 cut(s) 246, 649, 1402
NspV TTCGAA 1 cut(s) 1623
PaqCI CACCTGC 1 cut(s) 247
PceI AGGCCT 1 cut(s) 52
PciSI GCTCTTC 2 cut(s) 1389, 1491
PdmI GAANNNNTTC 5 cut(s) 415, 485, 1569, 1593, 1962
PfeI GAWTC 7 cut(s) 164, 850, 936, 1278, 1570, 2023, 2054
PfoI TCCNGGA 1 cut(s) 1735
PkrI GCNGC 4 cut(s) 1168, 1978, 2080, 2179
PleI GAGTC 6 cut(s) 24, 958, 1070, 1144, 1362, 1461
PmaCI CACGTG 1 cut(s) 2030
PmlI CACGTG 1 cut(s) 2030
PpsI GAGTC 6 cut(s) 24, 958, 1070, 1144, 1362, 1461
Ppu21I YACGTR 2 cut(s) 673, 2030
PsiI TTATAA 2 cut(s) 564, 974
Psp6I CCWGG 4 cut(s) 679, 861, 887, 1735
PspCI CACGTG 1 cut(s) 2030
PspGI CCWGG 4 cut(s) 679, 861, 887, 1735
PspN4I GGNNCC 6 cut(s) 205, 678, 700, 1025, 2041, 2255
PspPI GGNCC 2 cut(s) 585, 2254
PstI CTGCAG 2 cut(s) 1089, 1981
RsaI GTAC 6 cut(s) 471, 700, 1025, 2034, 2063, 2108
RsaNI GTAC 6 cut(s) 470, 699, 1024, 2033, 2062, 2107
RseI CAYNNNNRTG 1 cut(s) 241
SalI GTCGAC 1 cut(s) 764
SapI GCTCTTC 2 cut(s) 1389, 1491
SaqAI TTAA 9 cut(s) 405, 579, 770, 1047, 1103, 1179, 1326, 1820, 1946
SatI GCNGC 4 cut(s) 1167, 1977, 2079, 2178
Sau3AI GATC 8 cut(s) 277, 358, 795, 824, 1663, 1902, 2167, 2192
Sau96I GGNCC 2 cut(s) 585, 2254
SchI GAGTC 6 cut(s) 25, 959, 1070, 1145, 1362, 1462
ScrFI CCNGG 4 cut(s) 681, 863, 889, 1737
SfaNI GCATC 5 cut(s) 379, 448, 1054, 1666, 2221
SfcI CTRYAG 2 cut(s) 1085, 1977
SfuI TTCGAA 1 cut(s) 1623
SmiMI CAYNNNNRTG 1 cut(s) 241
SmlI CTYRAG 2 cut(s) 1865, 2188
SmoI CTYRAG 2 cut(s) 1865, 2188
SseBI AGGCCT 1 cut(s) 52
SsiI CCGC 7 cut(s) 527, 859, 1095, 1450, 1796, 2020, 2180
SspI AATATT 3 cut(s) 376, 600, 1256
SspMI CTAG 4 cut(s) 48, 57, 687, 1143
StuI AGGCCT 1 cut(s) 52
StyD4I CCNGG 4 cut(s) 679, 861, 887, 1735
StyI CCWWGG 2 cut(s) 748, 1347
TaaI ACNGT 5 cut(s) 35, 1269, 1530, 1807, 1992
TaiI ACGT 4 cut(s) 307, 675, 2005, 2032
TaqI TCGA 5 cut(s) 765, 832, 1060, 1623, 1986
TatI WGTACW 1 cut(s) 2032
TfiI GAWTC 7 cut(s) 164, 850, 936, 1278, 1570, 2023, 2054
Tru1I TTAA 9 cut(s) 405, 579, 770, 1047, 1103, 1179, 1326, 1820, 1946
Tru9I TTAA 9 cut(s) 405, 579, 770, 1047, 1103, 1179, 1326, 1820, 1946
TscAI CASTG 8 cut(s) 175, 290, 624, 1272, 1475, 1912, 2002, 2255
TseI GCWGC 4 cut(s) 1166, 1976, 2078, 2177
TspDTI ATGAA 5 cut(s) 102, 619, 1575, 1737, 2067
TspGWI ACGGA 3 cut(s) 638, 1441, 2049
TspRI CASTG 8 cut(s) 175, 290, 624, 1272, 1475, 1912, 2002, 2255
XapI RAATTY 6 cut(s) 964, 1405, 1579, 1625, 1725, 2088
XbaI TCTAGA 1 cut(s) 686
XceI RCATGY 3 cut(s) 246, 649, 1402
XmiI GTMKAC 4 cut(s) 192, 522, 714, 765
XmnI GAANNNNTTC 5 cut(s) 415, 485, 1569, 1593, 1962
XspI CTAG 4 cut(s) 48, 57, 687, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.