Rh4BG096100

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000678
Physical Location & Seq
Forward (+)
16413193 .. 16416354
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG096100.1

Sequence Viewer

Length: 822 bp
ATGGCCTACCTCCTCTCAAAAGATTCAAACTTATGCAGCAACCAGCACCGCCACCACCTTGATGATGTCATTTTGCCCATTCTTCCTGTCAAGAAGAGAAATGAGACTAGGGACCAGCCCATTCTCCCTAACCCAACTCCTGCCACCTTCGCCGTCACCACCTATTTGTTGTCGGCGAAGAAGAGGGTGTGGGCACTTCCACCCGATTTGTTCCCCAAGAAGCCAGTTTCGGAGCTGGATTTGAATCTTGATTCAGCTTTGACTAGCTCCATAATATTCTGTATGGTGATGCTAAAGGATGGTTTAAAAGAACCTGTTCTTTTCTCTTGGACAAATTTTTTTATAAACACTCTCACTTTCTTGTTGGTTGGCCATGTTATTGGGTCATGCTGGTACCTCCTTGGCTTACAGAGGGTTAATCAATGTCTTCGAGATGCCTGCCATAACTCTAATCTGCAGTCATGCGGGAGTTTAATAGATTGTTTTAATGCTCAATCTATTCCAGAGTCAACTAAATGGAGAGAAAATGAGGAGGCTGCTGGTTGTTTTAATCCGGAAGGAGATTTCTCCTATGGAGTATATACTCCAGTTGTCAATCTCACTACAAGAGATAGCATGACTAAATATTTATACTCACTGTTTTGGGGATTCCAGCAAATCAGCACTCTGGCAGGAAATCAAACTCCAAGCTATTTTAATGGAGAAATTGTTTTTACCATGGTAATTATTGGACTGGGACTCTTCTTCTTTGCTCTTCTAATTGGAAATATGCAGAATTTTCTTCGGTCTCTTGGACGGAGAGCTGTCATAAGCATGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

273

Amino Acids

30.81

Weight (kDa)

6.94

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 106 - 260 5.2e-10 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000678)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17700 AT3G17700
fragaria_vesca FvH4_5g18080 FvH4_5g18080 FvH4_5g18080 FvH4_6g44780
malus_domestica MD06G1236300.v1.1 MD09G1093300.v1.1 MD14G1243200.v1.1
prunus_persica Prupe.1G183800_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.3G231900_v2.0.a1 Prupe.5G240800_v2.0.a1 Prupe.5G240800_v2.0.a1 Prupe.5G240800_v2.0.a1
pyrus_communis pycom06g21110 pycom06g21140 pycom06g21190 pycom14g20450
rosa_chinensis RchiOBHm_Chr2g0161941 RchiOBHm_Chr4g0441651 RchiOBHm_Chr5g0083381 RchiOBHm_Chr6g0281101 RchiOBHm_Chr7g0177531
rosa_laevigata RLG00000005528 RLG00000015203 RLG00000015445 RLG00000021309 RLG00000023742 RLG00000034422 RLG00000036136
rosa_multiflora Rmu_co8432259.1_g000001 Rmu_co8481019.1_g000001 Rmu_sc0003410.1_g000009 Rmu_sc0004618.1_g000030 Rmu_sc0005144.1_g000007 Rmu_sc0008565.1_g000001 Rmu_sc0018381.1_g000003 Rmu_ssc0000330.1_g000004
rosa_roxburghii Rroxscaffold_1G00051700 Rroxscaffold_2G00088450 Rroxscaffold_2G00116130 Rroxscaffold_2G00126580 Rroxscaffold_3G00275780 Rroxscaffold_5G00338690 Rroxscaffold_5G00375080
rosa_rugosa Rorug02G0493400 Rorug04G0058900 Rorug04G0059000 Rorug06G0407100
rosa_samantha Rh2AG220400 Rh2AG282700 Rh2AG329100 Rh2AG559300 Rh2BG572700 Rh2CG542900 Rh2DG582500 Rh4AG135300 Rh4BG096100 Rh4DG026800 Rh4DG329100 Rh5CG271500 Rh6DG096900 Rh7AG006400 Rh7BG006300 Rh7CG006800 Rh7DG006400 Rh7DG278200
rosa_wichuraiana Rw0G014790 Rw1G017310 Rw7G000530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 344
Acc65I GGTACC 1 cut(s) 393
AccB1I GGYRCC 1 cut(s) 393
AccIII TCCGGA 1 cut(s) 553
AciI CCGC 2 cut(s) 49, 465
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 2 cut(s) 334, 775
AfaI GTAC 1 cut(s) 395
AgsI TTSAA 2 cut(s) 27, 244
AluBI AGCT 5 cut(s) 235, 257, 267, 690, 803
AluI AGCT 5 cut(s) 235, 257, 267, 690, 803
Alw26I GTCTC 2 cut(s) 98, 792
Aor13HI TCCGGA 1 cut(s) 553
AoxI GGCC 2 cut(s) 3, 370
ApeKI GCWGC 2 cut(s) 36, 536
ApoI RAATTY 2 cut(s) 334, 775
Asp700I GAANNNNTTC 1 cut(s) 315
Asp718I GGTACC 1 cut(s) 393
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 2 cut(s) 148, 298
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 196
BalI TGGCCA 1 cut(s) 372
BanI GGYRCC 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 48, 523
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 137
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BcoDI GTCTC 2 cut(s) 98, 792
BfaI CTAG 2 cut(s) 108, 264
BfmI CTRYAG 1 cut(s) 455
BisI GCNGC 2 cut(s) 37, 537
BlsI GCNGC 2 cut(s) 38, 538
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 113, 395
BmrI ACTGGG 1 cut(s) 743
BmsI GCATC 2 cut(s) 279, 424
BmuI ACTGGG 1 cut(s) 743
BpiI GAAGAC 1 cut(s) 419
BpmI CTGGAG 1 cut(s) 570
BsaBI GATNNNNATC 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 792
BsaJI CCNNGG 2 cut(s) 400, 717
BsaWI WCCGGW 1 cut(s) 553
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bse1I ACTGG 3 cut(s) 224, 587, 738
Bse8I GATNNNNATC 1 cut(s) 243
BseAI TCCGGA 1 cut(s) 553
BseDI CCNNGG 2 cut(s) 400, 717
BseGI GGATG 1 cut(s) 304
BseJI GATNNNNATC 1 cut(s) 243
BseNI ACTGG 3 cut(s) 224, 587, 738
BseRI GAGGAG 1 cut(s) 545
BseSI GKGCMC 1 cut(s) 196
BseXI GCAGC 2 cut(s) 48, 523
BshFI GGCC 2 cut(s) 5, 372
BshNI GGYRCC 1 cut(s) 393
BsiSI CCGG 1 cut(s) 554
BslFI GGGAC 2 cut(s) 125, 750
BsmAI GTCTC 2 cut(s) 98, 792
BsmFI GGGAC 2 cut(s) 125, 750
BsnI GGCC 2 cut(s) 5, 372
Bso31I GGTCTC 1 cut(s) 792
Bsp1286I GDGCHC 1 cut(s) 196
Bsp13I TCCGGA 1 cut(s) 553
Bsp19I CCATGG 1 cut(s) 717
BspACI CCGC 2 cut(s) 49, 465
BspANI GGCC 2 cut(s) 5, 372
BspEI TCCGGA 1 cut(s) 553
BspLI GGNNCC 2 cut(s) 113, 395
BspMAI CTGCAG 1 cut(s) 459
BspQI GCTCTTC 1 cut(s) 759
BspT107I GGYRCC 1 cut(s) 393
BspTNI GGTCTC 1 cut(s) 792
BsrI ACTGG 3 cut(s) 224, 587, 738
BssECI CCNNGG 2 cut(s) 400, 717
BssT1I CCWWGG 2 cut(s) 400, 717
Bst4CI ACNGT 1 cut(s) 639
Bst6I CTCTTC 4 cut(s) 89, 176, 746, 759
BstC8I GCNNGC 1 cut(s) 439
BstDSI CCRYGG 1 cut(s) 717
BstF5I GGATG 1 cut(s) 304
BstMAI GTCTC 2 cut(s) 98, 792
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 455
BstSLI GKGCMC 1 cut(s) 196
BstV1I GCAGC 2 cut(s) 48, 523
BstV2I GAAGAC 1 cut(s) 419
BstXI CCANNNNNNTGG 1 cut(s) 380
BsuRI GGCC 2 cut(s) 5, 372
BtgI CCRYGG 1 cut(s) 717
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 635
Cac8I GCNNGC 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 394
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 6 cut(s) 374, 387, 462, 616, 718, 814
CviQI GTAC 1 cut(s) 394
DraI TTTAAA 1 cut(s) 306
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 4 cut(s) 89, 176, 746, 759
EarI CTCTTC 4 cut(s) 89, 176, 746, 759
Eco130I CCWWGG 2 cut(s) 400, 717
Eco31I GGTCTC 1 cut(s) 792
Eco47I GGWCC 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 400, 717
ErhI CCWWGG 2 cut(s) 400, 717
FaeI CATG 6 cut(s) 377, 390, 465, 619, 721, 817
FaqI GGGAC 2 cut(s) 125, 750
FatI CATG 6 cut(s) 373, 386, 461, 615, 717, 813
FauI CCCGC 1 cut(s) 458
Fnu4HI GCNGC 2 cut(s) 37, 537
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 37, 537
FspBI CTAG 2 cut(s) 108, 264
GluI GCNGC 2 cut(s) 37, 537
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 2 cut(s) 5, 372
HapII CCGG 1 cut(s) 554
Hin1II CATG 6 cut(s) 377, 390, 465, 619, 721, 817
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HinfI GANTC 6 cut(s) 23, 244, 251, 506, 648, 738
HpaII CCGG 1 cut(s) 554
HphI GGTGA 2 cut(s) 148, 298
Hpy166II GTNNAC 1 cut(s) 510
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 5 cut(s) 91, 248, 431, 503, 554
Hpy8I GTNNAC 1 cut(s) 510
HpyAV CCTTC 2 cut(s) 157, 551
HpyCH4III ACNGT 1 cut(s) 639
HpyCH4V TGCA 3 cut(s) 36, 457, 772
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
Hsp92II CATG 6 cut(s) 377, 390, 465, 619, 721, 817
Kpn2I TCCGGA 1 cut(s) 553
KpnI GGTACC 1 cut(s) 397
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 2 cut(s) 232, 272
Lsp1109I GCAGC 2 cut(s) 48, 523
LweI GCATC 2 cut(s) 279, 424
MaeI CTAG 2 cut(s) 108, 264
MaeIII GTNAC 1 cut(s) 154
MboII GAAGA 9 cut(s) 74, 106, 190, 193, 419, 733, 736, 746, 773
MhlI GDGCHC 1 cut(s) 196
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 5 cut(s) 334, 705, 723, 759, 775
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 2 cut(s) 515, 732
MnlI CCTC 7 cut(s) 20, 23, 177, 405, 407, 523, 526
Mox20I TGGCCA 1 cut(s) 372
MroI TCCGGA 1 cut(s) 553
MroXI GAANNNNTTC 1 cut(s) 315
MscI TGGCCA 1 cut(s) 372
MseI TTAA 6 cut(s) 305, 417, 473, 486, 549, 696
MslI CAYNNNNRTG 2 cut(s) 60, 812
Msp20I TGGCCA 1 cut(s) 372
MspI CCGG 1 cut(s) 554
MwoI GCNNNNNNNGC 1 cut(s) 149
NcoI CCATGG 1 cut(s) 717
NlaIII CATG 6 cut(s) 377, 390, 465, 619, 721, 817
NlaIV GGNNCC 2 cut(s) 113, 395
NmuCI GTSAC 1 cut(s) 154
PciSI GCTCTTC 1 cut(s) 759
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 4 cut(s) 23, 244, 251, 648
PkrI GCNGC 2 cut(s) 38, 538
PleI GAGTC 2 cut(s) 514, 732
PpsI GAGTC 2 cut(s) 514, 732
PsiI TTATAA 1 cut(s) 344
PspN4I GGNNCC 2 cut(s) 113, 395
PspPI GGNCC 1 cut(s) 112
PstI CTGCAG 1 cut(s) 459
RsaI GTAC 1 cut(s) 395
RsaNI GTAC 1 cut(s) 394
RseI CAYNNNNRTG 2 cut(s) 60, 812
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 6 cut(s) 305, 417, 473, 486, 549, 696
SatI GCNGC 2 cut(s) 37, 537
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 2 cut(s) 515, 732
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 2 cut(s) 279, 424
SfcI CTRYAG 1 cut(s) 455
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 2 cut(s) 60, 812
Sse9I AATT 5 cut(s) 334, 705, 723, 759, 775
SsiI CCGC 2 cut(s) 49, 465
SspI AATATT 2 cut(s) 276, 626
SspMI CTAG 2 cut(s) 108, 264
StyI CCWWGG 2 cut(s) 400, 717
TaaI ACNGT 1 cut(s) 639
TaqI TCGA 1 cut(s) 430
TaqII GACCGA 1 cut(s) 774
TasI AATT 5 cut(s) 334, 705, 723, 759, 775
TfiI GAWTC 4 cut(s) 23, 244, 251, 648
Tru1I TTAA 6 cut(s) 305, 417, 473, 486, 549, 696
Tru9I TTAA 6 cut(s) 305, 417, 473, 486, 549, 696
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 2 cut(s) 36, 536
Tsp45I GTSAC 1 cut(s) 154
TspGWI ACGGA 1 cut(s) 811
TspRI CASTG 1 cut(s) 642
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 2 cut(s) 334, 775
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 2 cut(s) 108, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.