Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000857

Physical Location & Seq

Genomic Coordinates Forward (+)

3920643 .. 3924116

Gene Structure 3474 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1036500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGAAAGTTGAGACCTTCAATGCCGCTTTCTCTTCCGATGTTCGTACATTTCACAGGCTTCCGAGTTCTTCAATTTTCGGCGCAAAAAGCCCTAAAATTTTGACTGCCAGACCTTCCATGGCCTCCGGATTCACCCGGCGGCTTTCGATCTATTGCGCCGTTCGCTTCCGGCCTTGCATTGACATACACAAGGGTAAAGTGAAACAAATTGTTGGGTCTACCCTTTCGGATTCGAAGGATGATGGGTCGTTTCTTGTTACCAATTTTGTGTCGGATAAGTCCGCGGCGGAGTATGCAAAGATGTATAAAGAAGATGGGCTTAGAGGTGGTCATGTGATCATGCTTGGAGCTGACACTTTTAGTAGAGCTGCAGCCATTGAAGCTTTGCATGCCTATCCTGGTGGCTTGCAAGTAGGAGGTGGGATCAATTCAGAAAATTGTTTGCATTACATAGAAGAAGGGGCTAGCCATGTCATTGTCACCTCCTATGTATTTAACAATGGACAAATGGACCTTCAAAGGCTTAAAGATCTTGTTCTTGTTGTTGGAAAAGAGAGGCTTGTTTTAGACCTTAGCTGCAGAAAGAGGGATGGTAAATATGCAATCGTCACGGATAGATGGCAGAAGTTCAGTGATGTGAATCTTGATGAGGAAATATTGAATTTTCTTGCCAACTTTGCAGATGAATTTCTGGTACATGGTGTTGATGTTGAAGGGAAAAAGCTGGGCATTGATGAGGAGCTTGTGGCGTTGCTTGGAAAGCATTCACCGATTCCTGTAACTTATGCTGGTGGTGTGACTGTAATGGCTGATTTAGAGAGGATTAAAGTGGCAGGACTGGGACACGTAGATGTTACTGTTGGCAGCGCTTTGGATGTTTTTGGAGGCAACTTGTCATACAAAGACGTCGTTGCTTGGCATGCCCAGCAGGAGGCCTTGACTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000105 GO:0000162 GO:0003674 GO:0003824 GO:0003949 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006547 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0018130 GO:0019438 GO:0019752 GO:0034641 GO:0042401 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046219 GO:0046394 GO:0046483 GO:0052803 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230

Pfam Domains

Protein Families

His_biosynth

Protein Analysis

316

Amino Acids

34.31

Weight (kDa)

6.37

Isoelectric Point (pI)

36.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
His_biosynth	PF00977	58 - 291	4.4e-29	Histidine biosynthesis protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36230
fragaria_vesca	FvH4_6g18790 FvH4_6g18790 FvH4_6g18790 FvH4_6g18790 FvH4_6g18790 FvH4_6g18790
malus_domestica	MD12G1036500.v1.1 MD14G1023300.v1.1
prunus_persica	Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1
pyrus_communis	pycom12g02400 pycom14g02220 pycom14g02230
rosa_chinensis	RchiOBHm_Chr1g0324901 RchiOBHm_Chr1g0350151 RchiOBHm_Chr3g0473801 RchiOBHm_Chr4g0393091 RchiOBHm_Chr4g0409701 RchiOBHm_Chr5g0027171
rosa_laevigata	RLG00000014193 RLG00000023983 RLG00000035737
rosa_multiflora	Rmu_sc0001207.1_g000028 Rmu_sc0002986.1_g000013 Rmu_sc0007035.1_g000005 Rmu_sc0016122.1_g000001 Rmu_sc0037919.1_g000001
rosa_roxburghii	Rroxscaffold_1G00029230 Rroxscaffold_1G00029310 Rroxscaffold_2G00114250 Rroxscaffold_3G00260930 Rroxscaffold_3G00260940 Rroxscaffold_4G00311820 Rroxscaffold_4G00332560 Rroxscaffold_5G00338520 Rroxscaffold_5G00355640 Rroxscaffold_6G00407790
rosa_rugosa	Rorug01G0107500 Rorug02G0045900 Rorug03G0135600 Rorug06G0225600 Rorug07G0319400 Rorug07G0330900
rosa_samantha	Rh1AG080400 Rh1AG119900 Rh1AG120000 Rh1AG233100 Rh1CG115000 Rh1CG217700 Rh2BG241500 Rh2BG353000 Rh2DG556200 Rh3AG186100 Rh3AG202300 Rh3BG214400 Rh3CG211200 Rh3DG210400 Rh3DG227900 Rh5AG343700 Rh5DG191000
rosa_wichuraiana	Rw1G018100 Rw3G017000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	909
AccI	GTMKAC	1 cut(s)	218
AccII	CGCG	1 cut(s)	284
AccIII	TCCGGA	1 cut(s)	125
AciI	CCGC	5 cut(s)	24, 139, 282, 284, 287
AclWI	GGATC	1 cut(s)	431
AcsI	RAATTY	3 cut(s)	96, 661, 686
AcyI	GRCGYC	1 cut(s)	906
AfaI	GTAC	2 cut(s)	46, 696
AfeI	AGCGCT	1 cut(s)	868
AfiI	CCNNNNNNNGG	1 cut(s)	931
AflIII	ACRYGT	1 cut(s)	844
AgsI	TTSAA	6 cut(s)	19, 72, 380, 518, 661, 713
AjnI	CCWGG	1 cut(s)	397
AluBI	AGCT	6 cut(s)	350, 368, 383, 576, 724, 742
AluI	AGCT	6 cut(s)	350, 368, 383, 576, 724, 742
Alw26I	GTCTC	1 cut(s)	5
AlwI	GGATC	1 cut(s)	431
Aor13HI	TCCGGA	1 cut(s)	125
Aor51HI	AGCGCT	1 cut(s)	868
AoxI	GGCC	3 cut(s)	120, 170, 933
ApeKI	GCWGC	4 cut(s)	368, 371, 576, 864
ApoI	RAATTY	3 cut(s)	96, 661, 686
Asp700I	GAANNNNTTC	1 cut(s)	763
AspLEI	GCGC	3 cut(s)	83, 158, 869
AspS9I	GGNCC	1 cut(s)	511
AsuC2I	CCSGG	1 cut(s)	136
AsuHPI	GGTGA	3 cut(s)	124, 472, 759
AsuII	TTCGAA	1 cut(s)	233
AsuNHI	GCTAGC	1 cut(s)	464
AvaII	GGWCC	1 cut(s)	511
BbvI	GCAGC	4 cut(s)	355, 383, 563, 876
BccI	CCATC	4 cut(s)	236, 308, 584, 612
BceAI	ACGGC	1 cut(s)	143
BciT130I	CCWGG	1 cut(s)	399
BclI	TGATCA	1 cut(s)	336
BcnI	CCSGG	1 cut(s)	136
BcoDI	GTCTC	1 cut(s)	5
BfaI	CTAG	1 cut(s)	465
BfmI	CTRYAG	2 cut(s)	369, 577
BfoI	RGCGCY	1 cut(s)	870
BglII	AGATCT	1 cut(s)	529
BisI	GCNGC	7 cut(s)	24, 140, 285, 369, 372, 577, 865
BlsI	GCNGC	7 cut(s)	25, 141, 286, 370, 373, 578, 866
Bme1390I	CCNGG	2 cut(s)	136, 399
Bme18I	GGWCC	1 cut(s)	511
BmgT120I	GGNCC	1 cut(s)	511
BmrFI	CCNGG	2 cut(s)	136, 399
BmrI	ACTGGG	1 cut(s)	848
BmtI	GCTAGC	1 cut(s)	468
BmuI	ACTGGG	1 cut(s)	848
Bpu10I	CCTNAGC	1 cut(s)	572
Bpu14I	TTCGAA	1 cut(s)	233
BpuMI	CCSGG	1 cut(s)	136
BsaAI	YACGTR	1 cut(s)	847
BsaBI	GATNNNNATC	1 cut(s)	639
BsaHI	GRCGYC	1 cut(s)	906
BsaI	GGTCTC	1 cut(s)	5
BsaJI	CCNNGG	2 cut(s)	117, 282
BsaWI	WCCGGW	1 cut(s)	125
Bsc4I	CCNNNNNNNGG	1 cut(s)	931
Bse1I	ACTGG	1 cut(s)	843
Bse8I	GATNNNNATC	1 cut(s)	639
BseAI	TCCGGA	1 cut(s)	125
BseBI	CCWGG	1 cut(s)	399
BseDI	CCNNGG	2 cut(s)	117, 282
BseGI	GGATG	3 cut(s)	244, 595, 880
BseJI	GATNNNNATC	1 cut(s)	639
BseLI	CCNNNNNNNGG	1 cut(s)	931
BseNI	ACTGG	1 cut(s)	843
BseRI	GAGGAG	1 cut(s)	752
BseXI	GCAGC	4 cut(s)	355, 383, 563, 876
BseYI	CCCAGC	2 cut(s)	724, 924
Bsh1236I	CGCG	1 cut(s)	284
BshFI	GGCC	3 cut(s)	122, 172, 935
BsiSI	CCGG	3 cut(s)	126, 136, 169
BslFI	GGGAC	1 cut(s)	855
BslI	CCNNNNNNNGG	1 cut(s)	931
BsmAI	GTCTC	1 cut(s)	5
BsmFI	GGGAC	1 cut(s)	855
BsmI	GAATGC	1 cut(s)	763
BsnI	GGCC	3 cut(s)	122, 172, 935
Bso31I	GGTCTC	1 cut(s)	5
Bsp119I	TTCGAA	1 cut(s)	233
Bsp13I	TCCGGA	1 cut(s)	125
Bsp143I	GATC	4 cut(s)	147, 336, 423, 529
Bsp19I	CCATGG	1 cut(s)	117
BspACI	CCGC	5 cut(s)	24, 139, 282, 284, 287
BspANI	GGCC	3 cut(s)	122, 172, 935
BspEI	TCCGGA	1 cut(s)	125
BspFNI	CGCG	1 cut(s)	284
BspMAI	CTGCAG	2 cut(s)	373, 581
BspOI	GCTAGC	1 cut(s)	468
BspPI	GGATC	1 cut(s)	431
BspT104I	TTCGAA	1 cut(s)	233
BspTNI	GGTCTC	1 cut(s)	5
BsrI	ACTGG	1 cut(s)	843
BssECI	CCNNGG	2 cut(s)	117, 282
BssMI	GATC	4 cut(s)	147, 336, 423, 529
BssNI	GRCGYC	1 cut(s)	906
BssT1I	CCWWGG	1 cut(s)	117
Bst2UI	CCWGG	1 cut(s)	399
Bst4CI	ACNGT	3 cut(s)	802, 859, 943
Bst6I	CTCTTC	1 cut(s)	37
BstACI	GRCGYC	1 cut(s)	906
BstBAI	YACGTR	1 cut(s)	847
BstBI	TTCGAA	1 cut(s)	233
BstC8I	GCNNGC	4 cut(s)	390, 407, 466, 921
BstDEI	CTNAG	2 cut(s)	320, 572
BstDSI	CCRYGG	2 cut(s)	117, 282
BstF5I	GGATG	3 cut(s)	244, 595, 880
BstFNI	CGCG	1 cut(s)	284
BstH2I	RGCGCY	1 cut(s)	870
BstHHI	GCGC	3 cut(s)	83, 158, 869
BstKTI	GATC	4 cut(s)	150, 339, 426, 532
BstMAI	GTCTC	1 cut(s)	5
BstMBI	GATC	4 cut(s)	147, 336, 423, 529
BstMWI	GCNNNNNNNGC	9 cut(s)	87, 162, 293, 380, 389, 677, 760, 920, 925
BstNI	CCWGG	1 cut(s)	399
BstNSI	RCATGY	2 cut(s)	392, 923
BstSCI	CCNGG	2 cut(s)	134, 397
BstSFI	CTRYAG	2 cut(s)	369, 577
BstUI	CGCG	1 cut(s)	284
BstV1I	GCAGC	4 cut(s)	355, 383, 563, 876
BstX2I	RGATCY	1 cut(s)	529
BstYI	RGATCY	1 cut(s)	529
BsuRI	GGCC	3 cut(s)	122, 172, 935
BtgI	CCRYGG	2 cut(s)	117, 282
BtsCI	GGATG	3 cut(s)	244, 595, 880
BtsIMutI	CAGTG	1 cut(s)	637
Cac8I	GCNNGC	4 cut(s)	390, 407, 466, 921
CfoI	GCGC	3 cut(s)	83, 158, 869
Cfr13I	GGNCC	1 cut(s)	511
Cfr42I	CCGCGG	1 cut(s)	285
Csp6I	GTAC	2 cut(s)	45, 695
CviAII	CATG	7 cut(s)	118, 332, 340, 389, 470, 698, 920
CviQI	GTAC	2 cut(s)	45, 695
DdeI	CTNAG	2 cut(s)	320, 572
DpnI	GATC	4 cut(s)	149, 338, 425, 531
DpnII	GATC	4 cut(s)	147, 336, 423, 529
Eam1104I	CTCTTC	1 cut(s)	37
EarI	CTCTTC	1 cut(s)	37
EciI	GGCGGA	1 cut(s)	302
Eco130I	CCWWGG	1 cut(s)	117
Eco147I	AGGCCT	1 cut(s)	935
Eco31I	GGTCTC	1 cut(s)	5
Eco47I	GGWCC	1 cut(s)	511
Eco47III	AGCGCT	1 cut(s)	868
EcoRII	CCWGG	1 cut(s)	397
EcoT14I	CCWWGG	1 cut(s)	117
ErhI	CCWWGG	1 cut(s)	117
FaeI	CATG	7 cut(s)	121, 335, 343, 392, 473, 701, 923
FalI	AAGNNNNNCTT	4 cut(s)	303, 335, 543, 575
FaqI	GGGAC	1 cut(s)	855
FatI	CATG	7 cut(s)	117, 331, 339, 388, 469, 697, 919
FbaI	TGATCA	1 cut(s)	336
FblI	GTMKAC	1 cut(s)	218
Fnu4HI	GCNGC	7 cut(s)	24, 140, 285, 369, 372, 577, 865
FokI	GGATG	3 cut(s)	251, 602, 887
Fsp4HI	GCNGC	7 cut(s)	24, 140, 285, 369, 372, 577, 865
FspBI	CTAG	1 cut(s)	465
GlaI	GCGC	3 cut(s)	82, 157, 868
GluI	GCNGC	7 cut(s)	24, 140, 285, 369, 372, 577, 865
GsaI	CCCAGC	2 cut(s)	728, 928
HaeII	RGCGCY	1 cut(s)	870
HaeIII	GGCC	3 cut(s)	122, 172, 935
HapII	CCGG	3 cut(s)	126, 136, 169
HhaI	GCGC	3 cut(s)	83, 158, 869
Hin1I	GRCGYC	1 cut(s)	906
Hin1II	CATG	7 cut(s)	121, 335, 343, 392, 473, 701, 923
Hin6I	GCGC	3 cut(s)	81, 156, 867
HinP1I	GCGC	3 cut(s)	81, 156, 867
HindIII	AAGCTT	1 cut(s)	381
HinfI	GANTC	4 cut(s)	129, 230, 640, 772
HpaII	CCGG	3 cut(s)	126, 136, 169
HphI	GGTGA	3 cut(s)	124, 472, 759
Hpy166II	GTNNAC	1 cut(s)	219
Hpy188I	TCNGA	5 cut(s)	37, 63, 229, 274, 433
Hpy188III	TCNNGA	2 cut(s)	126, 644
Hpy8I	GTNNAC	1 cut(s)	219
Hpy99I	CGWCG	1 cut(s)	911
HpyAV	CCTTC	6 cut(s)	25, 123, 229, 452, 524, 707
HpyCH4III	ACNGT	3 cut(s)	802, 859, 943
HpyCH4IV	ACGT	2 cut(s)	846, 906
HpyCH4V	TGCA	9 cut(s)	177, 296, 371, 388, 409, 445, 579, 602, 680
HpyF10VI	GCNNNNNNNGC	9 cut(s)	87, 162, 293, 380, 389, 677, 760, 920, 925
HpyF3I	CTNAG	2 cut(s)	320, 572
HpySE526I	ACGT	2 cut(s)	846, 906
Hsp92I	GRCGYC	1 cut(s)	906
Hsp92II	CATG	7 cut(s)	121, 335, 343, 392, 473, 701, 923
HspAI	GCGC	3 cut(s)	81, 156, 867
Kpn2I	TCCGGA	1 cut(s)	125
Ksp22I	TGATCA	1 cut(s)	336
KspI	CCGCGG	1 cut(s)	285
Kzo9I	GATC	4 cut(s)	147, 336, 423, 529
LmnI	GCTCC	2 cut(s)	347, 739
Lsp1109I	GCAGC	4 cut(s)	355, 383, 563, 876
MaeI	CTAG	1 cut(s)	465
MaeII	ACGT	2 cut(s)	846, 906
MaeIII	GTNAC	6 cut(s)	256, 478, 607, 778, 796, 853
MalI	GATC	4 cut(s)	149, 338, 425, 531
MboI	GATC	4 cut(s)	147, 336, 423, 529
MboII	GAAGA	4 cut(s)	24, 60, 323, 467
MflI	RGATCY	1 cut(s)	529
MluCI	AATT	8 cut(s)	72, 96, 207, 262, 427, 436, 661, 686
MmeI	TCCRAC	2 cut(s)	252, 526
MroI	TCCGGA	1 cut(s)	125
MroXI	GAANNNNTTC	1 cut(s)	763
MseI	TTAA	3 cut(s)	495, 525, 825
MslI	CAYNNNNRTG	1 cut(s)	849
MspA1I	CMGCKG	1 cut(s)	284
MspI	CCGG	3 cut(s)	126, 136, 169
MspR9I	CCNGG	2 cut(s)	136, 399
Mva1269I	GAATGC	1 cut(s)	763
MvaI	CCWGG	1 cut(s)	399
MvnI	CGCG	1 cut(s)	284
MwoI	GCNNNNNNNGC	9 cut(s)	87, 162, 293, 380, 389, 677, 760, 920, 925
NciI	CCSGG	1 cut(s)	136
NcoI	CCATGG	1 cut(s)	117
NdeII	GATC	4 cut(s)	147, 336, 423, 529
NheI	GCTAGC	1 cut(s)	464
NlaIII	CATG	7 cut(s)	121, 335, 343, 392, 473, 701, 923
NmuCI	GTSAC	3 cut(s)	478, 607, 796
NspI	RCATGY	2 cut(s)	392, 923
NspV	TTCGAA	1 cut(s)	233
PaeI	GCATGC	2 cut(s)	392, 923
PceI	AGGCCT	1 cut(s)	935
PctI	GAATGC	1 cut(s)	763
PdmI	GAANNNNTTC	1 cut(s)	763
PfeI	GAWTC	4 cut(s)	129, 230, 640, 772
PkrI	GCNGC	7 cut(s)	25, 141, 286, 370, 373, 578, 866
Ppu21I	YACGTR	1 cut(s)	847
Psp6I	CCWGG	1 cut(s)	397
PspFI	CCCAGC	2 cut(s)	724, 924
PspGI	CCWGG	1 cut(s)	397
PspPI	GGNCC	1 cut(s)	511
PstI	CTGCAG	2 cut(s)	373, 581
PsuI	RGATCY	1 cut(s)	529
RsaI	GTAC	2 cut(s)	46, 696
RsaNI	GTAC	2 cut(s)	45, 695
RseI	CAYNNNNRTG	1 cut(s)	849
SacII	CCGCGG	1 cut(s)	285
SaqAI	TTAA	3 cut(s)	495, 525, 825
SatI	GCNGC	7 cut(s)	24, 140, 285, 369, 372, 577, 865
Sau3AI	GATC	4 cut(s)	147, 336, 423, 529
Sau96I	GGNCC	1 cut(s)	511
ScrFI	CCNGG	2 cut(s)	136, 399
SfcI	CTRYAG	2 cut(s)	369, 577
Sfr303I	CCGCGG	1 cut(s)	285
SfuI	TTCGAA	1 cut(s)	233
SgrBI	CCGCGG	1 cut(s)	285
SinI	GGWCC	1 cut(s)	511
SmiMI	CAYNNNNRTG	1 cut(s)	849
SphI	GCATGC	2 cut(s)	392, 923
Sse9I	AATT	8 cut(s)	72, 96, 207, 262, 427, 436, 661, 686
SseBI	AGGCCT	1 cut(s)	935
SsiI	CCGC	5 cut(s)	24, 139, 282, 284, 287
SspI	AATATT	1 cut(s)	657
SspMI	CTAG	1 cut(s)	465
StuI	AGGCCT	1 cut(s)	935
StyD4I	CCNGG	2 cut(s)	134, 397
StyI	CCWWGG	1 cut(s)	117
TaaI	ACNGT	3 cut(s)	802, 859, 943
TaiI	ACGT	2 cut(s)	849, 909
TaqI	TCGA	2 cut(s)	146, 233
TasI	AATT	8 cut(s)	72, 96, 207, 262, 427, 436, 661, 686
TauI	GCSGC	3 cut(s)	26, 142, 287
TfiI	GAWTC	4 cut(s)	129, 230, 640, 772
Tru1I	TTAA	3 cut(s)	495, 525, 825
Tru9I	TTAA	3 cut(s)	495, 525, 825
TscAI	CASTG	1 cut(s)	637
TseFI	GTSAC	3 cut(s)	478, 607, 796
TseI	GCWGC	4 cut(s)	368, 371, 576, 864
Tsp45I	GTSAC	3 cut(s)	478, 607, 796
TspDTI	ATGAA	2 cut(s)	17, 699
TspGWI	ACGGA	1 cut(s)	626
TspRI	CASTG	1 cut(s)	637
VpaK11BI	GGWCC	1 cut(s)	511
XapI	RAATTY	3 cut(s)	96, 661, 686
XceI	RCATGY	2 cut(s)	392, 923
XcmI	CCANNNNNNNNNTGG	1 cut(s)	115
XmiI	GTMKAC	1 cut(s)	218
XmnI	GAANNNNTTC	1 cut(s)	763
XspI	CTAG	1 cut(s)	465
ZraI	GACGTC	1 cut(s)	907

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.