Rroxscaffold_2G00114250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000857
Physical Location & Seq
Reverse (-)
40161978 .. 40162796
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00114250.1

Sequence Viewer

Length: 351 bp
ATGGGGATGATCGGGGGAGGAGGAGATCACCATGGTGGGAAGGATCGGCCCATGCAACGCCGAAATGGTGTTTTCCGCCGCGGGTCGGGTTTCTCGCACACGGGGCTTCTTCGATCCGCGTCCGGGGGCAGCGACGAGCAAAGCAGTTGCACCGGGCGTTGGGCGGCTTGGTCCGAGCACAAGGAGAGCCGGGTCGAGGCCGAGGCCTGGATGGTGGATGGTGTTGGTGAGGGTCTTAAGCTCGACAAGGTTTTGAGAGAGAGAAAGCTCCTCGATCTGGTTTTGTTTCTTCTTCTTCTTACCCGGGTTGCAGGCTTGGGGGTGGTGTGGGAGATTAGGACCGAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.69

Weight (kDa)

9.03

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 81, 119
AciI CCGC 5 cut(s) 76, 79, 81, 117, 164
AclWI GGATC 2 cut(s) 51, 108
AfiI CCNNNNNNNGG 6 cut(s) 85, 123, 159, 196, 207, 277
AflII CTTAAG 1 cut(s) 236
AjnI CCWGG 1 cut(s) 206
AleI CACNNNNGTG 1 cut(s) 33
AluBI AGCT 2 cut(s) 241, 268
AluI AGCT 2 cut(s) 241, 268
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 2 cut(s) 51, 108
Ama87I CYCGRG 1 cut(s) 303
AoxI GGCC 4 cut(s) 47, 198, 204, 345
ApeKI GCWGC 1 cut(s) 129
AspS9I GGNCC 3 cut(s) 48, 171, 339
AsuC2I CCSGG 5 cut(s) 124, 154, 191, 304, 305
AsuHPI GGTGA 2 cut(s) 20, 239
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 2 cut(s) 171, 339
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 141
BccI CCATC 2 cut(s) 205, 212
BciT130I CCWGG 1 cut(s) 208
BcnI CCSGG 5 cut(s) 124, 154, 191, 304, 305
BfaI CTAG 1 cut(s) 349
BfrI CTTAAG 1 cut(s) 236
BisI GCNGC 3 cut(s) 79, 130, 165
BlsI GCNGC 3 cut(s) 80, 131, 166
Bme1390I CCNGG 6 cut(s) 124, 154, 191, 208, 304, 305
Bme18I GGWCC 2 cut(s) 171, 339
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 3 cut(s) 48, 171, 339
BmrFI CCNGG 6 cut(s) 124, 154, 191, 208, 304, 305
BpuMI CCSGG 5 cut(s) 124, 154, 191, 304, 305
BsaJI CCNNGG 6 cut(s) 31, 79, 123, 201, 303, 342
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 123, 159, 196, 207, 277
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 6 cut(s) 31, 79, 123, 201, 303, 342
BseGI GGATG 3 cut(s) 12, 216, 223
BseLI CCNNNNNNNGG 6 cut(s) 85, 123, 159, 196, 207, 277
BseRI GAGGAG 3 cut(s) 33, 36, 260
BseXI GCAGC 1 cut(s) 141
Bsh1236I CGCG 2 cut(s) 81, 119
BshFI GGCC 4 cut(s) 49, 200, 206, 347
BsiHKAI GWGCWC 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 4 cut(s) 123, 153, 190, 304
BslI CCNNNNNNNGG 6 cut(s) 85, 123, 159, 196, 207, 277
BsnI GGCC 4 cut(s) 49, 200, 206, 347
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 5 cut(s) 9, 25, 43, 113, 274
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 5 cut(s) 76, 79, 81, 117, 164
BspANI GGCC 4 cut(s) 49, 200, 206, 347
BspFNI CGCG 2 cut(s) 81, 119
BspPI GGATC 2 cut(s) 51, 108
BspTI CTTAAG 1 cut(s) 236
BssECI CCNNGG 6 cut(s) 31, 79, 123, 201, 303, 342
BssMI GATC 5 cut(s) 9, 25, 43, 113, 274
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 208
BstAFI CTTAAG 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 313
BstDSI CCRYGG 2 cut(s) 31, 79
BstF5I GGATG 3 cut(s) 12, 216, 223
BstFNI CGCG 2 cut(s) 81, 119
BstKTI GATC 5 cut(s) 12, 28, 46, 116, 277
BstMBI GATC 5 cut(s) 9, 25, 43, 113, 274
BstMWI GCNNNNNNNGC 1 cut(s) 103
BstNI CCWGG 1 cut(s) 208
BstSCI CCNGG 6 cut(s) 122, 152, 189, 206, 302, 303
BstUI CGCG 2 cut(s) 81, 119
BstV1I GCAGC 1 cut(s) 141
BsuRI GGCC 4 cut(s) 49, 200, 206, 347
BtgI CCRYGG 2 cut(s) 31, 79
BtsCI GGATG 3 cut(s) 12, 216, 223
Cac8I GCNNGC 1 cut(s) 313
Cfr13I GGNCC 3 cut(s) 48, 171, 339
Cfr42I CCGCGG 1 cut(s) 82
Cfr9I CCCGGG 1 cut(s) 303
CseI GACGC 1 cut(s) 108
CviAII CATG 2 cut(s) 32, 52
DpnI GATC 5 cut(s) 11, 27, 45, 115, 276
DpnII GATC 5 cut(s) 9, 25, 43, 113, 274
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 2 cut(s) 206, 347
Eco47I GGWCC 2 cut(s) 171, 339
Eco88I CYCGRG 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 206
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 2 cut(s) 35, 55
FaiI YATR 2 cut(s) 33, 53
FatI CATG 2 cut(s) 31, 51
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 3 cut(s) 79, 130, 165
FokI GGATG 3 cut(s) 19, 223, 230
Fsp4HI GCNGC 3 cut(s) 79, 130, 165
FspBI CTAG 1 cut(s) 349
GluI GCNGC 3 cut(s) 79, 130, 165
HaeIII GGCC 4 cut(s) 49, 200, 206, 347
HapII CCGG 4 cut(s) 123, 153, 190, 304
HgaI GACGC 1 cut(s) 108
Hin1II CATG 2 cut(s) 35, 55
HpaII CCGG 4 cut(s) 123, 153, 190, 304
HphI GGTGA 2 cut(s) 20, 239
Hpy188I TCNGA 1 cut(s) 175
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 34
HpyCH4V TGCA 3 cut(s) 55, 150, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 103
Hsp92II CATG 2 cut(s) 35, 55
KspI CCGCGG 1 cut(s) 82
Kzo9I GATC 5 cut(s) 9, 25, 43, 113, 274
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 8 cut(s) 136, 166, 193, 203, 220, 263, 297, 317
Lsp1109I GCAGC 1 cut(s) 141
MaeI CTAG 1 cut(s) 349
MalI GATC 5 cut(s) 11, 27, 45, 115, 276
MboI GATC 5 cut(s) 9, 25, 43, 113, 274
MboII GAAGA 4 cut(s) 101, 281, 284, 287
MhlI GDGCHC 1 cut(s) 180
MnlI CCTC 7 cut(s) 11, 14, 190, 196, 223, 281, 337
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 81
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 4 cut(s) 123, 153, 190, 304
MspR9I CCNGG 6 cut(s) 124, 154, 191, 208, 304, 305
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 2 cut(s) 81, 119
MwoI GCNNNNNNNGC 1 cut(s) 103
NciI CCSGG 5 cut(s) 124, 154, 191, 304, 305
NcoI CCATGG 1 cut(s) 31
NdeII GATC 5 cut(s) 9, 25, 43, 113, 274
NlaIII CATG 2 cut(s) 35, 55
NmeAIII GCCGAG 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 33
PceI AGGCCT 2 cut(s) 206, 347
PkrI GCNGC 3 cut(s) 80, 131, 166
Psp6I CCWGG 1 cut(s) 206
PspGI CCWGG 1 cut(s) 206
PspPI GGNCC 3 cut(s) 48, 171, 339
RseI CAYNNNNRTG 1 cut(s) 33
SacII CCGCGG 1 cut(s) 82
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 3 cut(s) 79, 130, 165
Sau3AI GATC 5 cut(s) 9, 25, 43, 113, 274
Sau96I GGNCC 3 cut(s) 48, 171, 339
ScrFI CCNGG 6 cut(s) 124, 154, 191, 208, 304, 305
SduI GDGCHC 1 cut(s) 180
SetI ASST 3 cut(s) 243, 252, 270
Sfr303I CCGCGG 1 cut(s) 82
SgrBI CCGCGG 1 cut(s) 82
SinI GGWCC 2 cut(s) 171, 339
SmaI CCCGGG 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
SseBI AGGCCT 2 cut(s) 206, 347
SsiI CCGC 5 cut(s) 76, 79, 81, 117, 164
SspMI CTAG 1 cut(s) 349
StuI AGGCCT 2 cut(s) 206, 347
StyD4I CCNGG 6 cut(s) 122, 152, 189, 206, 302, 303
StyI CCWWGG 1 cut(s) 31
TaqI TCGA 4 cut(s) 112, 195, 243, 273
TauI GCSGC 2 cut(s) 81, 167
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TseI GCWGC 1 cut(s) 129
TspMI CCCGGG 1 cut(s) 303
Vha464I CTTAAG 1 cut(s) 236
VpaK11BI GGWCC 2 cut(s) 171, 339
XmaI CCCGGG 1 cut(s) 303
XspI CTAG 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.