Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0000857

Physical Location & Seq

Genomic Coordinates Forward (+)

36230644 .. 36235349

Gene Structure 4706 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0330900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGACTTATCATCATCCTCCCACCCGCAATCCAACCTCTCTTTGGGATTTTTATCCCACGCGCCGTCGTCTCAAATCGCCGATGACTCCATCGCACTCCCCGTAGCCCCATCAATCCCGCTTCAATTGCTCGAGCCACAGCCAGAGAGCCACGCAACAACAACAACAACAACTAATGGTGAAATTGGCGACGACGACGACGACAGAGACGTCGAGGAGTTTCGGATATTGGGGCATTCCATGTGCTTGAAACGCCGCAGGGACAGAGAATCCTCCTCCTGTTCCTCCACTAATTCCAAACGCGCGGCACTCTCACCGGACCTTGAGGCCCGGAGGACTGCTGTCCGGGCCTGGGGTGACCAGCCTCTCCGAGTTGCCGACTCCGATGTGTTTGAGATTATGGACAAGGAGAAGCAGAGGCAGTTCAGAGGGATTGAATTGATTGCCTCGGAGAATTTCGTGTGCCGGGCAGTAATGGAAGCACTTGGAAGCCACTTGACCAATAAGTATTCCGAAGGAATGCCTGGTGCAAGGTACTACGGTGGTAATCAGTACATTGATGAGATCGAAACACTTTGCTGTAAGCGTGCTTTAGCCGCATTTGGTCTCGATGCTGAGAATTGGGGTGTCAATGTGCAGCCTTATTCATGTACATCTGCCAACTTTGCTGTGTACACTGGATTGTTATTGCCCGGTGACAGAATAATGGGGTTGGATACCCCATCTGGAGGGAATACTAGTCATGGATATTATACCAGCAATGGGAGGAAAGTTTCCGGAGCATCAATTTTCTTTGAGACCTTGCCTTATAAGGTCAATTTACAAACTGGGTATATTGATTATGATAAGCTTGAGGAGAGGGCGCTTGATTTTCGCCCCAAGATACTTATTTGTGGTGGGAGTTCGTATCCCCGCGAATGGGATTATGCAAGGTTTAGGCAGATTGCTGATAAATGTGGAGCTGTGCTGCTTTGTGATATGGCGCAAATTAGTGGGCTTATTGCAGCAAAGGAATGTGTAAATCCTTTTGACTACTGTGACATTGTTACTTCAACTACTCATAAAAGCCTTCGGGGTCCCAGGGGAGGTATAATTTTTTATAGGAAGGGTACAAAACCAAGGAAGAGGGGATTTCTTTTAACTCAAGGAAGTGAAAGCGACCACTATGATTTTGAGGAAAAGATCAACTTTGCCGTTTTTCCATCATTGCAAGGGGGACCGCACAATAACCACATTGCTGCTCTTGCCATAGCTTTAAAGCAGGTGGCTACACCTGAATACAAGGCATATATGCAGCAAGTGAAGAAAAATGCACAGGCTTTAGCATCATCTTTGCTGAGAAGAAAGTGCAGGTTAGTAACTGGTGGCACAGACAACCATTTGCTACTTTGGGATCTGAGGCCCCTAGGATTATCAGGCAAGAACTACGAGAAGGTATGTGAAATGTGTCACATTACTGTCAACAAGATCGCCATTTTTGGTGATAATGGAACTATTACTCCTGGAGGAGTGAGGATTGGTACCCCTGCTATGACTTCAAGAGGTTGTTTGGAGATGGATTTTGAGACAATTGCTGATTTTCTCCTAAGAGCTGCACAGATTGCAACTTTAGTGCAAAGGGAGCATGGGAAGTTGCAGAAGCCTTTTCTGAAGGGTCTTGAGAGCAATAAGGATATTGTTGAGCTTAGGACCAGAGTTGAAACCTTTGCTTCTCAGTTTGCAATGCCGGGATATGATATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000105 GO:0000162 GO:0003674 GO:0003824 GO:0003949 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006547 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016853 GO:0016860 GO:0016861 GO:0018130 GO:0019438 GO:0019752 GO:0034641 GO:0042401 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046219 GO:0046394 GO:0046483 GO:0052803 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00340 ko01100 ko01110 ko01230 map00340 map01100 map01110 map01230

Pfam Domains

Protein Families

His_biosynth

Protein Analysis

580

Amino Acids

64.14

Weight (kDa)

6.68

Isoelectric Point (pI)

44.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	124 - 527	6.2e-170	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36230
fragaria_vesca	FvH4_6g18790 FvH4_6g18790 FvH4_6g18790 FvH4_6g18790 FvH4_6g18790 FvH4_6g18790
malus_domestica	MD12G1036500.v1.1 MD14G1023300.v1.1
prunus_persica	Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1 Prupe.7G109700_v2.0.a1
pyrus_communis	pycom12g02400 pycom14g02220 pycom14g02230
rosa_chinensis	RchiOBHm_Chr1g0324901 RchiOBHm_Chr1g0350151 RchiOBHm_Chr3g0473801 RchiOBHm_Chr4g0393091 RchiOBHm_Chr4g0409701 RchiOBHm_Chr5g0027171
rosa_laevigata	RLG00000014193 RLG00000023983 RLG00000035737
rosa_multiflora	Rmu_sc0001207.1_g000028 Rmu_sc0002986.1_g000013 Rmu_sc0007035.1_g000005 Rmu_sc0016122.1_g000001 Rmu_sc0037919.1_g000001
rosa_roxburghii	Rroxscaffold_1G00029230 Rroxscaffold_1G00029310 Rroxscaffold_2G00114250 Rroxscaffold_3G00260930 Rroxscaffold_3G00260940 Rroxscaffold_4G00311820 Rroxscaffold_4G00332560 Rroxscaffold_5G00338520 Rroxscaffold_5G00355640 Rroxscaffold_6G00407790
rosa_rugosa	Rorug01G0107500 Rorug02G0045900 Rorug03G0135600 Rorug06G0225600 Rorug07G0319400 Rorug07G0330900
rosa_samantha	Rh1AG080400 Rh1AG119900 Rh1AG120000 Rh1AG233100 Rh1CG115000 Rh1CG217700 Rh2BG241500 Rh2BG353000 Rh2DG556200 Rh3AG186100 Rh3AG202300 Rh3BG214400 Rh3CG211200 Rh3DG210400 Rh3DG227900 Rh5AG343700 Rh5DG191000
rosa_wichuraiana	Rw1G018100 Rw3G017000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	810
AarI	CACCTGC	1 cut(s)	1252
AasI	GACNNNNNNGTC	1 cut(s)	209
AatII	GACGTC	1 cut(s)	213
Acc36I	ACCTGC	2 cut(s)	1252, 1341
Acc65I	GGTACC	1 cut(s)	1520
AccB1I	GGYRCC	1 cut(s)	1520
AccII	CGCG	4 cut(s)	62, 303, 305, 915
AccIII	TCCGGA	1 cut(s)	776
AciI	CCGC	7 cut(s)	26, 119, 256, 305, 597, 913, 1220
AclWI	GGATC	1 cut(s)	1401
AcsI	RAATTY	1 cut(s)	454
AcuI	CTGAAG	1 cut(s)	1670
AcyI	GRCGYC	1 cut(s)	210
AfaI	GTAC	6 cut(s)	536, 554, 652, 674, 1111, 1522
AfiI	CCNNNNNNNGG	7 cut(s)	43, 352, 728, 762, 918, 919, 1085
AgsI	TTSAA	6 cut(s)	125, 250, 437, 1053, 1539, 1700
AhlI	ACTAGT	1 cut(s)	737
AjnI	CCWGG	4 cut(s)	350, 523, 1079, 1501
AloI	GAACNNNNNNTCC	2 cut(s)	1483, 1515
AluBI	AGCT	5 cut(s)	850, 962, 1253, 1592, 1684
AluI	AGCT	5 cut(s)	850, 962, 1253, 1592, 1684
Alw26I	GTCTC	5 cut(s)	75, 201, 611, 791, 1559
AlwI	GGATC	1 cut(s)	1401
Ama87I	CYCGRG	1 cut(s)	131
Aor13HI	TCCGGA	1 cut(s)	776
AoxI	GGCC	3 cut(s)	327, 348, 1400
ApeKI	GCWGC	6 cut(s)	637, 967, 1004, 1238, 1294, 1592
ApoI	RAATTY	1 cut(s)	454
Asp718I	GGTACC	1 cut(s)	1520
AspA2I	CCTAGG	1 cut(s)	1405
AspLEI	GCGC	4 cut(s)	64, 305, 865, 985
AspS9I	GGNCC	7 cut(s)	319, 328, 348, 1076, 1217, 1401, 1689
AsuC2I	CCSGG	5 cut(s)	331, 347, 467, 693, 1728
AsuHPI	GGTGA	5 cut(s)	191, 306, 368, 707, 1493
AvaI	CYCGRG	1 cut(s)	131
AvaII	GGWCC	4 cut(s)	319, 1076, 1217, 1689
AvrII	CCTAGG	1 cut(s)	1405
BaeI	ACNNNNGTAYC	2 cut(s)	526, 559
BanI	GGYRCC	1 cut(s)	1520
BbvI	GCAGC	6 cut(s)	649, 954, 1016, 1225, 1306, 1579
BccI	CCATC	5 cut(s)	98, 118, 730, 1210, 1549
BceAI	ACGGC	2 cut(s)	49, 1178
BciT130I	CCWGG	4 cut(s)	352, 525, 1081, 1503
BciVI	GTATCC	2 cut(s)	709, 918
BcnI	CCSGG	5 cut(s)	331, 347, 467, 693, 1728
BcoDI	GTCTC	5 cut(s)	75, 201, 611, 791, 1559
BcuI	ACTAGT	1 cut(s)	737
BfaI	CTAG	2 cut(s)	738, 1406
BfoI	RGCGCY	1 cut(s)	866
BfuAI	ACCTGC	2 cut(s)	1252, 1341
BfuI	GTATCC	2 cut(s)	709, 918
BisI	GCNGC	9 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295, 1593
BlnI	CCTAGG	1 cut(s)	1405
BlsI	GCNGC	9 cut(s)	257, 307, 598, 639, 969, 1006, 1240, 1296, 1594
Bme1390I	CCNGG	9 cut(s)	331, 347, 352, 467, 525, 693, 1081, 1503, 1728
Bme18I	GGWCC	4 cut(s)	319, 1076, 1217, 1689
BmeT110I	CYCGRG	1 cut(s)	131
BmgT120I	GGNCC	7 cut(s)	319, 328, 348, 1076, 1217, 1401, 1689
BmiI	GGNNCC	5 cut(s)	1077, 1078, 1218, 1403, 1522
BmrFI	CCNGG	9 cut(s)	331, 347, 352, 467, 525, 693, 1081, 1503, 1728
BmrI	ACTGGG	1 cut(s)	837
BmsI	GCATC	3 cut(s)	601, 791, 1334
BmuI	ACTGGG	1 cut(s)	837
BoxI	GACNNNNGTC	1 cut(s)	341
BpmI	CTGGAG	2 cut(s)	747, 1524
Bpu10I	CCTNAGC	1 cut(s)	1685
BpuEI	CTTGAG	4 cut(s)	344, 872, 1128, 1679
BpuMI	CCSGG	5 cut(s)	331, 347, 467, 693, 1728
BsaBI	GATNNNNATC	1 cut(s)	52
BsaHI	GRCGYC	1 cut(s)	210
BsaI	GGTCTC	2 cut(s)	611, 791
BsaJI	CCNNGG	6 cut(s)	351, 447, 1079, 1080, 1118, 1405
BsaWI	WCCGGW	2 cut(s)	316, 776
BsaXI	ACNNNNNCTCC	2 cut(s)	1483, 1513
Bsc4I	CCNNNNNNNGG	7 cut(s)	43, 352, 728, 762, 918, 919, 1085
Bse1I	ACTGG	3 cut(s)	682, 832, 1366
Bse3DI	GCAATG	4 cut(s)	766, 1205, 1233, 1728
Bse8I	GATNNNNATC	1 cut(s)	52
BseAI	TCCGGA	1 cut(s)	776
BseBI	CCWGG	4 cut(s)	352, 525, 1081, 1503
BseDI	CCNNGG	6 cut(s)	351, 447, 1079, 1080, 1118, 1405
BseGI	GGATG	1 cut(s)	14
BseJI	GATNNNNATC	1 cut(s)	52
BseLI	CCNNNNNNNGG	7 cut(s)	43, 352, 728, 762, 918, 919, 1085
BseMI	GCAATG	4 cut(s)	766, 1205, 1233, 1728
BseMII	CTCAG	4 cut(s)	606, 1328, 1388, 1727
BseNI	ACTGG	3 cut(s)	682, 832, 1366
BseRI	GAGGAG	4 cut(s)	230, 265, 869, 1521
BseXI	GCAGC	6 cut(s)	649, 954, 1016, 1225, 1306, 1579
BsgI	GTGCAG	3 cut(s)	656, 1369, 1578
Bsh1236I	CGCG	4 cut(s)	62, 303, 305, 915
BshFI	GGCC	3 cut(s)	329, 350, 1402
BshNI	GGYRCC	1 cut(s)	1520
BsiHKCI	CYCGRG	1 cut(s)	131
BsiSI	CCGG	7 cut(s)	317, 331, 346, 466, 693, 777, 1727
BslFI	GGGAC	3 cut(s)	275, 1062, 1230
BslI	CCNNNNNNNGG	7 cut(s)	43, 352, 728, 762, 918, 919, 1085
BsmAI	GTCTC	5 cut(s)	75, 201, 611, 791, 1559
BsmBI	CGTCTC	2 cut(s)	75, 201
BsmFI	GGGAC	3 cut(s)	275, 1062, 1230
BsmI	GAATGC	2 cut(s)	235, 525
BsnI	GGCC	3 cut(s)	329, 350, 1402
Bso31I	GGTCTC	2 cut(s)	611, 791
BsoBI	CYCGRG	1 cut(s)	131
Bsp13I	TCCGGA	1 cut(s)	776
Bsp1407I	TGTACA	2 cut(s)	650, 672
Bsp143I	GATC	4 cut(s)	564, 1182, 1393, 1467
BspACI	CCGC	7 cut(s)	26, 119, 256, 305, 597, 913, 1220
BspANI	GGCC	3 cut(s)	329, 350, 1402
BspCNI	CTCAG	4 cut(s)	607, 1329, 1389, 1726
BspEI	TCCGGA	1 cut(s)	776
BspFNI	CGCG	4 cut(s)	62, 303, 305, 915
BspLI	GGNNCC	5 cut(s)	1077, 1078, 1218, 1403, 1522
BspMI	ACCTGC	2 cut(s)	1252, 1341
BspPI	GGATC	1 cut(s)	1401
BspT107I	GGYRCC	1 cut(s)	1520
BspTNI	GGTCTC	2 cut(s)	611, 791
BsrDI	GCAATG	4 cut(s)	766, 1205, 1233, 1728
BsrGI	TGTACA	2 cut(s)	650, 672
BsrI	ACTGG	3 cut(s)	682, 832, 1366
BssECI	CCNNGG	6 cut(s)	351, 447, 1079, 1080, 1118, 1405
BssMI	GATC	4 cut(s)	564, 1182, 1393, 1467
BssNI	GRCGYC	1 cut(s)	210
BssT1I	CCWWGG	2 cut(s)	1118, 1405
Bst2UI	CCWGG	4 cut(s)	352, 525, 1081, 1503
Bst4CI	ACNGT	3 cut(s)	542, 1037, 1459
Bst6I	CTCTTC	1 cut(s)	1118
BstACI	GRCGYC	1 cut(s)	210
BstAPI	GCANNNNNTGC	1 cut(s)	1601
BstAUI	TGTACA	2 cut(s)	650, 672
BstC8I	GCNNGC	1 cut(s)	588
BstDEI	CTNAG	6 cut(s)	615, 1337, 1397, 1586, 1685, 1713
BstEII	GGTNACC	1 cut(s)	356
BstF5I	GGATG	1 cut(s)	14
BstFNI	CGCG	4 cut(s)	62, 303, 305, 915
BstH2I	RGCGCY	1 cut(s)	866
BstHHI	GCGC	4 cut(s)	64, 305, 865, 985
BstKTI	GATC	4 cut(s)	567, 1185, 1396, 1470
BstMAI	GTCTC	5 cut(s)	75, 201, 611, 791, 1559
BstMBI	GATC	4 cut(s)	564, 1182, 1393, 1467
BstMWI	GCNNNNNNNGC	8 cut(s)	127, 252, 347, 596, 665, 1244, 1601, 1621
BstNI	CCWGG	4 cut(s)	352, 525, 1081, 1503
BstPAI	GACNNNNGTC	1 cut(s)	341
BstPI	GGTNACC	1 cut(s)	356
BstSCI	CCNGG	9 cut(s)	329, 345, 350, 465, 523, 691, 1079, 1501, 1726
BstUI	CGCG	4 cut(s)	62, 303, 305, 915
BstV1I	GCAGC	6 cut(s)	649, 954, 1016, 1225, 1306, 1579
BstX2I	RGATCY	1 cut(s)	1393
BstYI	RGATCY	1 cut(s)	1393
BsuI	GTATCC	2 cut(s)	709, 918
BsuRI	GGCC	3 cut(s)	329, 350, 1402
BtgZI	GCGATG	1 cut(s)	76
BtsCI	GGATG	1 cut(s)	14
BtsIMutI	CAGTG	1 cut(s)	675
BveI	ACCTGC	2 cut(s)	1252, 1341
Cac8I	GCNNGC	1 cut(s)	588
CfoI	GCGC	4 cut(s)	64, 305, 865, 985
Cfr13I	GGNCC	7 cut(s)	319, 328, 348, 1076, 1217, 1401, 1689
Csp6I	GTAC	6 cut(s)	535, 553, 651, 673, 1110, 1521
CviAII	CATG	4 cut(s)	241, 648, 743, 1625
CviQI	GTAC	6 cut(s)	535, 553, 651, 673, 1110, 1521
DdeI	CTNAG	6 cut(s)	615, 1337, 1397, 1586, 1685, 1713
DpnI	GATC	4 cut(s)	566, 1184, 1395, 1469
DpnII	GATC	4 cut(s)	564, 1182, 1393, 1467
DraI	TTTAAA	1 cut(s)	1257
DrdI	GACNNNNNNGTC	1 cut(s)	209
DseDI	GACNNNNNNGTC	1 cut(s)	209
Eam1104I	CTCTTC	1 cut(s)	1118
EarI	CTCTTC	1 cut(s)	1118
Eco130I	CCWWGG	2 cut(s)	1118, 1405
Eco31I	GGTCTC	2 cut(s)	611, 791
Eco47I	GGWCC	4 cut(s)	319, 1076, 1217, 1689
Eco57I	CTGAAG	1 cut(s)	1670
Eco88I	CYCGRG	1 cut(s)	131
Eco91I	GGTNACC	1 cut(s)	356
EcoO109I	RGGNCCY	2 cut(s)	1076, 1401
EcoO65I	GGTNACC	1 cut(s)	356
EcoRII	CCWGG	4 cut(s)	350, 523, 1079, 1501
EcoT14I	CCWWGG	2 cut(s)	1118, 1405
ErhI	CCWWGG	2 cut(s)	1118, 1405
Esp3I	CGTCTC	2 cut(s)	75, 201
FaeI	CATG	4 cut(s)	244, 651, 746, 1628
FalI	AAGNNNNNCTT	2 cut(s)	1172, 1204
FaqI	GGGAC	3 cut(s)	275, 1062, 1230
FatI	CATG	4 cut(s)	240, 647, 742, 1624
FauI	CCCGC	3 cut(s)	33, 126, 920
Fnu4HI	GCNGC	9 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295, 1593
Fsp4HI	GCNGC	9 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295, 1593
FspBI	CTAG	2 cut(s)	738, 1406
GlaI	GCGC	4 cut(s)	63, 304, 864, 984
GluI	GCNGC	9 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295, 1593
GsuI	CTGGAG	2 cut(s)	747, 1524
HaeII	RGCGCY	1 cut(s)	866
HaeIII	GGCC	3 cut(s)	329, 350, 1402
HapII	CCGG	7 cut(s)	317, 331, 346, 466, 693, 777, 1727
HhaI	GCGC	4 cut(s)	64, 305, 865, 985
Hin1I	GRCGYC	1 cut(s)	210
Hin1II	CATG	4 cut(s)	244, 651, 746, 1628
Hin6I	GCGC	4 cut(s)	62, 303, 863, 983
HinP1I	GCGC	4 cut(s)	62, 303, 863, 983
HincII	GTYRAC	1 cut(s)	1462
HindII	GTYRAC	1 cut(s)	1462
HindIII	AAGCTT	1 cut(s)	848
HinfI	GANTC	3 cut(s)	86, 269, 380
HpaII	CCGG	7 cut(s)	317, 331, 346, 466, 693, 777, 1727
HphI	GGTGA	5 cut(s)	191, 306, 368, 707, 1493
Hpy166II	GTNNAC	3 cut(s)	673, 675, 1462
Hpy188I	TCNGA	8 cut(s)	225, 371, 385, 428, 451, 514, 1398, 1650
Hpy188III	TCNNGA	5 cut(s)	608, 726, 777, 1539, 1658
Hpy8I	GTNNAC	3 cut(s)	673, 675, 1462
Hpy99I	CGWCG	6 cut(s)	70, 194, 197, 200, 203, 215
HpyAV	CCTTC	5 cut(s)	509, 1079, 1099, 1426, 1645
HpyCH4III	ACNGT	3 cut(s)	542, 1037, 1459
HpyCH4IV	ACGT	1 cut(s)	210
HpyF10VI	GCNNNNNNNGC	8 cut(s)	127, 252, 347, 596, 665, 1244, 1601, 1621
HpyF3I	CTNAG	6 cut(s)	615, 1337, 1397, 1586, 1685, 1713
HpySE526I	ACGT	1 cut(s)	210
Hsp92I	GRCGYC	1 cut(s)	210
Hsp92II	CATG	4 cut(s)	244, 651, 746, 1628
HspAI	GCGC	4 cut(s)	62, 303, 863, 983
KflI	GGGWCCC	1 cut(s)	1076
Kpn2I	TCCGGA	1 cut(s)	776
KpnI	GGTACC	1 cut(s)	1524
Kzo9I	GATC	4 cut(s)	564, 1182, 1393, 1467
LmnI	GCTCC	3 cut(s)	779, 959, 1621
Lsp1109I	GCAGC	6 cut(s)	649, 954, 1016, 1225, 1306, 1579
LweI	GCATC	3 cut(s)	601, 791, 1334
MaeI	CTAG	2 cut(s)	738, 1406
MaeII	ACGT	1 cut(s)	210
MaeIII	GTNAC	6 cut(s)	356, 695, 1037, 1045, 1357, 1448
MalI	GATC	4 cut(s)	566, 1184, 1395, 1469
MboI	GATC	4 cut(s)	564, 1182, 1393, 1467
MboII	GAAGA	3 cut(s)	1135, 1315, 1353
MfeI	CAATTG	2 cut(s)	125, 1569
MflI	RGATCY	1 cut(s)	1393
MlyI	GAGTC	2 cut(s)	80, 374
MmeI	TCCRAC	2 cut(s)	57, 693
MroI	TCCGGA	1 cut(s)	776
MseI	TTAA	2 cut(s)	1139, 1256
MspI	CCGG	7 cut(s)	317, 331, 346, 466, 693, 777, 1727
MspR9I	CCNGG	9 cut(s)	331, 347, 352, 467, 525, 693, 1081, 1503, 1728
MunI	CAATTG	2 cut(s)	125, 1569
Mva1269I	GAATGC	2 cut(s)	235, 525
MvaI	CCWGG	4 cut(s)	352, 525, 1081, 1503
MvnI	CGCG	4 cut(s)	62, 303, 305, 915
MwoI	GCNNNNNNNGC	8 cut(s)	127, 252, 347, 596, 665, 1244, 1601, 1621
NciI	CCSGG	5 cut(s)	331, 347, 467, 693, 1728
NdeII	GATC	4 cut(s)	564, 1182, 1393, 1467
NlaIII	CATG	4 cut(s)	244, 651, 746, 1628
NlaIV	GGNNCC	5 cut(s)	1077, 1078, 1218, 1403, 1522
NmuCI	GTSAC	4 cut(s)	356, 695, 1037, 1448
PaeR7I	CTCGAG	1 cut(s)	131
PaqCI	CACCTGC	1 cut(s)	1252
PasI	CCCWGGG	1 cut(s)	1080
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 198, 207
PctI	GAATGC	2 cut(s)	235, 525
PfeI	GAWTC	1 cut(s)	269
PfoI	TCCNGGA	1 cut(s)	1501
PkrI	GCNGC	9 cut(s)	257, 307, 598, 639, 969, 1006, 1240, 1296, 1594
PleI	GAGTC	2 cut(s)	80, 374
PpsI	GAGTC	2 cut(s)	80, 374
PpuMI	RGGWCCY	1 cut(s)	1076
PshAI	GACNNNNGTC	1 cut(s)	341
PsiI	TTATAA	1 cut(s)	810
Psp5II	RGGWCCY	1 cut(s)	1076
Psp6I	CCWGG	4 cut(s)	350, 523, 1079, 1501
PspEI	GGTNACC	1 cut(s)	356
PspGI	CCWGG	4 cut(s)	350, 523, 1079, 1501
PspN4I	GGNNCC	5 cut(s)	1077, 1078, 1218, 1403, 1522
PspPI	GGNCC	7 cut(s)	319, 328, 348, 1076, 1217, 1401, 1689
PspPPI	RGGWCCY	1 cut(s)	1076
PspXI	VCTCGAGB	1 cut(s)	131
PsuI	RGATCY	1 cut(s)	1393
RsaI	GTAC	6 cut(s)	536, 554, 652, 674, 1111, 1522
RsaNI	GTAC	6 cut(s)	535, 553, 651, 673, 1110, 1521
SaqAI	TTAA	2 cut(s)	1139, 1256
SatI	GCNGC	9 cut(s)	256, 306, 597, 638, 968, 1005, 1239, 1295, 1593
Sau3AI	GATC	4 cut(s)	564, 1182, 1393, 1467
Sau96I	GGNCC	7 cut(s)	319, 328, 348, 1076, 1217, 1401, 1689
SchI	GAGTC	2 cut(s)	80, 374
ScrFI	CCNGG	9 cut(s)	331, 347, 352, 467, 525, 693, 1081, 1503, 1728
SfaNI	GCATC	3 cut(s)	601, 791, 1334
Sfr274I	CTCGAG	1 cut(s)	131
SinI	GGWCC	4 cut(s)	319, 1076, 1217, 1689
SlaI	CTCGAG	1 cut(s)	131
SmlI	CTYRAG	5 cut(s)	131, 323, 851, 1143, 1658
SmoI	CTYRAG	5 cut(s)	131, 323, 851, 1143, 1658
SpeI	ACTAGT	1 cut(s)	737
SsiI	CCGC	7 cut(s)	26, 119, 256, 305, 597, 913, 1220
SspMI	CTAG	2 cut(s)	738, 1406
StyD4I	CCNGG	9 cut(s)	329, 345, 350, 465, 523, 691, 1079, 1501, 1726
StyI	CCWWGG	2 cut(s)	1118, 1405
TaaI	ACNGT	3 cut(s)	542, 1037, 1459
TaiI	ACGT	1 cut(s)	213
TaqI	TCGA	4 cut(s)	132, 213, 567, 609
TatI	WGTACW	3 cut(s)	552, 650, 672
TauI	GCSGC	3 cut(s)	258, 308, 599
TfiI	GAWTC	1 cut(s)	269
Tru1I	TTAA	2 cut(s)	1139, 1256
Tru9I	TTAA	2 cut(s)	1139, 1256
TscAI	CASTG	1 cut(s)	682
TseFI	GTSAC	4 cut(s)	356, 695, 1037, 1448
TseI	GCWGC	6 cut(s)	637, 967, 1004, 1238, 1294, 1592
Tsp45I	GTSAC	4 cut(s)	356, 695, 1037, 1448
TspDTI	ATGAA	1 cut(s)	636
TspRI	CASTG	1 cut(s)	682
VpaK11BI	GGWCC	4 cut(s)	319, 1076, 1217, 1689
XapI	RAATTY	1 cut(s)	454
XcmI	CCANNNNNNNNNTGG	1 cut(s)	40
XhoI	CTCGAG	1 cut(s)	131
XmaJI	CCTAGG	1 cut(s)	1405
XspI	CTAG	2 cut(s)	738, 1406
ZraI	GACGTC	1 cut(s)	211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.