MD15G1436400.v1.1

methyltransferase DDB_G0268948

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000258
Physical Location & Seq
Reverse (-)
53645614 .. 53650350
4737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1436400.v1.1.491

Sequence Viewer

Length: 1422 bp
ATGGCAGGTTTATTTGACAAGCAGGCAGAAGATTACTTGGTAGCAAGGCCAACTTACCCCAAGGAATGGTACTCCATGCTGGCGGCTCTCACACCAAAACACTCTTTAGCTTGGGATGTCGGCACCGGCAATGGCCAAGCTGCTTTCTCTCTTGGAGAGCATTATGAGCAAGTAATAGGAAGTGATATAAGTGAATCCCAACTGAAGAGTGCACTCCAACATCCTCGAGTTCGTTACGTTCACACTCCGGTGACCATGTCCGATGACGAGTTGGTTGACCTAATAGGGGGCGAAAACTCAGTTGATTTAGTGACCGTGGCAGAGGCCGTGCACTGGTTTGACCTCCCAAAGTTCTACTCGCTCGTCAAGAGCATTTTGTATGTCTACAAGTTAGACAAATTTGATGGATCAAATTTCACCCGATGGAAGGACAAGATGCGTTTCATGCTTACTGTTCTAAATGTCATATATGTGTTGGACCCGAAATTAGCGCCTATTCGTGAACCAAGTGACAAAGACTCGGACAATATAGTTGCCTATCGAAAGAAGCGTGAAGAAGATGAATTCGTATGTCGAGGACACATCTTGGGCACATTATCTGATCGTTTATATGACCTGTATGCACACATTCAATCTTCAAAGGAAATTTGGGAAGCACTTGAACATAAGTACACAACGGAAAAAAGAGGTACCGATAAATTTTTAATGTTCAAATATTATGAATTCACTATGTTTGATAACAAACCCATCCTAGACCAAGTTCATGAATTATTGGTCTTGGTCTCAAAGCTTCGTGAACTTAAATTAGTTATTCCGGATCCTATGATAGTTGGGGCTATTATGGCAAAATTACCCCAAACATGGAATAACTATAGAAAGAAACTTCTACACATGGTGGAAGATATTAGTTTAGAGAATTTGCAAAAGGGTATTCAAATTGAAGAGGAAACTCGAAATCGTGATAAGAATTTTGCAAATCAAGATTTCTCTAAAGTTCACATTGTCGAAGGAAACAAATATAAAAAGAACTTCAAAGTCAAAATTGACAAAGGGAAGTTTAAGAAGACCAATTCCAACAACAATCAAAAGAATGCAAATGGTCGTTTTTATGAAGCAAGTAAACCGTTTTGGGACCCGAAGAGAGAGTACTTGTGGGAAGGGTATAGGACACTTCCATTTCCATTTGAGAGTGTTGGTTTGGGTTCTGAAGGGCAGCCTGTGGCACTGGAAATACTAAAGGAAGTGTCATTTGAAGGGGTGTTGAGGATGTTAAGGTCCTCCTCTGCAGTCAATACAGCTAAGGATCAAGGCGTGGATTTGTTATCACAAGAGGTGATTGAAGAGATTCAAAGAGCTTGGGGAAGGCCTAACGTGGTCAGAAATGTTACCTTCAAGGCATTTACGCTTGCCGGAAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

474

Amino Acids

54.65

Weight (kDa)

7.72

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 39 - 127 2.3e-08 Methyltransferase domain
Methyltransf_11 PF08241 39 - 118 2.8e-08 Methyltransferase domain
Methyltransf_12 PF08242 39 - 115 1.1e-06 Methyltransferase domain
Retrotran_gag_2 PF14223 146 - 322 7.9e-30 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00980
fragaria_vesca FvH4_1g25682 FvH4_2g13961 FvH4_2g20162 FvH4_2g24753 FvH4_3g12191 FvH4_3g12632 FvH4_3g16171 FvH4_4g05871 FvH4_4g05873 FvH4_4g10121 FvH4_4g15488 FvH4_4g23911 FvH4_4g27782 FvH4_5g08912 FvH4_5g23001 FvH4_5g24552 FvH4_5g32801 FvH4_5g32802 FvH4_6g05093 FvH4_6g19722 FvH4_6g20391 FvH4_6g33792 FvH4_7g01381 FvH4_7g16761 FvH4_7g16761 FvH4_7g16761
malus_domestica MD01G1082500.v1.1 MD15G1436400.v1.1
prunus_persica Prupe.2G021400_v2.0.a1 Prupe.2G052400_v2.0.a1 Prupe.2G065000_v2.0.a1 Prupe.2G187700_v2.0.a1 Prupe.2G187800_v2.0.a1 Prupe.2G189900_v2.0.a1 Prupe.2G191300_v2.0.a1 Prupe.2G191700_v2.0.a1 Prupe.4G265600_v2.0.a1 Prupe.7G014200_v2.0.a1 Prupe.7G039000_v2.0.a1
pyrus_communis pycom01g05590 pycom01g05910 pycom02g12230 pycom02g25840 pycom03g15580 pycom04g04480 pycom05g09230 pycom05g23350 pycom05g23360 pycom07g08380 pycom09g01980 pycom09g01990 pycom11g18190 pycom11g18200 pycom11g21580 pycom13g04160 pycom15g25800 pycom16g16210 pycom16g17230 pycom16g24560 pycom16g25700 pycom17g11020
rosa_chinensis RchiOBHm_Chr1g0359981 RchiOBHm_Chr2g0151961 RchiOBHm_Chr2g0171331 RchiOBHm_Chr5g0081471 RchiOBHm_Chr6g0283031 RchiOBHm_Chr7g0197111 RchiOBHm_Chr7g0222601
rosa_laevigata RLG00000027843 RLG00000027845 RLG00000030065
rosa_multiflora Rmu_sc0000381.1_g000031 Rmu_sc0001179.1_g000035 Rmu_sc0001616.1_g000046 Rmu_sc0001851.1_g000032 Rmu_sc0002152.1_g000013 Rmu_sc0004031.1_g000015 Rmu_sc0004645.1_g000003 Rmu_sc0007393.1_g000001 Rmu_sc0008949.1_g000003 Rmu_sc0009799.1_g000011 Rmu_sc0011614.1_g000001 Rmu_sc0011699.1_g000009 Rmu_sc0019280.1_g000001 Rmu_sc0023984.1_g000001 Rmu_ssc0000050.1_g000036
rosa_roxburghii Rroxscaffold_1G00022650 Rroxscaffold_1G00033480 Rroxscaffold_3G00270250 Rroxscaffold_4G00296600 Rroxscaffold_4G00296760 Rroxscaffold_6G00409840
rosa_rugosa Rorug01G0273800 Rorug01G0273900 Rorug01G0274000
rosa_samantha Rh1AG287300 Rh1AG288100 Rh1BG253000 Rh1BG253500 Rh1BG399800 Rh1CG270400 Rh1CG271000 Rh1DG282000 Rh1DG282500 Rh4DG126500 Rh5DG194600 Rh5DG461100
rosa_wichuraiana Rw0G009130 Rw1G002590 Rw1G008490 Rw1G012210 Rw1G014290 Rw1G014490 Rw1G025510 Rw2G025840 Rw2G026170 Rw2G036510 Rw3G003050 Rw4G009270 Rw5G002350 Rw5G013930 Rw5G019500 Rw5G025890 Rw5G029960 Rw5G032360 Rw6G009890 Rw6G015670 Rw6G015860 Rw7G010030 Rw7G032600 Rw7G032770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 689
AccB1I GGYRCC 2 cut(s) 122, 689
AccB7I CCANNNNNTGG 1 cut(s) 66
AccI GTMKAC 1 cut(s) 384
AccIII TCCGGA 1 cut(s) 814
AciI CCGC 1 cut(s) 83
AclWI GGATC 4 cut(s) 415, 812, 825, 1311
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 8 cut(s) 398, 412, 563, 645, 698, 722, 916, 967
AcuI CTGAAG 2 cut(s) 224, 1227
AdeI CACNNNGTG 1 cut(s) 895
AfaI GTAC 4 cut(s) 71, 671, 691, 1148
AfiI CCNNNNNNNGG 5 cut(s) 66, 286, 333, 427, 861
AleI CACNNNNGTG 1 cut(s) 248
AluBI AGCT 5 cut(s) 110, 140, 790, 1298, 1355
AluI AGCT 5 cut(s) 110, 140, 790, 1298, 1355
Alw21I GWGCWC 2 cut(s) 214, 333
Alw26I GTCTC 1 cut(s) 787
Alw44I GTGCAC 2 cut(s) 210, 329
AlwI GGATC 4 cut(s) 415, 812, 825, 1311
Ama87I CYCGRG 1 cut(s) 225
Aor13HI TCCGGA 1 cut(s) 814
AoxI GGCC 4 cut(s) 47, 133, 324, 1364
ApaLI GTGCAC 2 cut(s) 210, 329
ApeKI GCWGC 2 cut(s) 140, 1213
ApoI RAATTY 8 cut(s) 398, 412, 563, 645, 698, 722, 916, 967
Asp700I GAANNNNTTC 1 cut(s) 1344
Asp718I GGTACC 1 cut(s) 689
AspLEI GCGC 1 cut(s) 493
AspS9I GGNCC 3 cut(s) 478, 1132, 1275
AsuHPI GGTGA 3 cut(s) 262, 409, 1345
AvaI CYCGRG 1 cut(s) 225
AvaII GGWCC 3 cut(s) 478, 1132, 1275
BaeGI GKGCMC 3 cut(s) 214, 333, 593
BalI TGGCCA 1 cut(s) 135
BamHI GGATCC 1 cut(s) 817
BanI GGYRCC 2 cut(s) 122, 689
BarI GAAGNNNNNNTAC 2 cut(s) 1130, 1162
BbsI GAAGAC 1 cut(s) 1070
Bbv12I GWGCWC 2 cut(s) 214, 333
BbvI GCAGC 2 cut(s) 127, 1225
BccI CCATC 3 cut(s) 398, 417, 755
BceAI ACGGC 1 cut(s) 311
BcoDI GTCTC 1 cut(s) 787
BfaI CTAG 1 cut(s) 752
BfmI CTRYAG 2 cut(s) 871, 1284
BfoI RGCGCY 1 cut(s) 494
BisI GCNGC 3 cut(s) 84, 141, 1214
BlsI GCNGC 3 cut(s) 85, 142, 1215
BmcAI AGTACT 1 cut(s) 1148
Bme18I GGWCC 3 cut(s) 478, 1132, 1275
BmeT110I CYCGRG 1 cut(s) 225
BmgT120I GGNCC 3 cut(s) 478, 1132, 1275
BmiI GGNNCC 6 cut(s) 124, 480, 691, 819, 1133, 1134
BmsI GCATC 1 cut(s) 426
BpiI GAAGAC 1 cut(s) 1070
Bpu10I CCTNAGC 1 cut(s) 1299
BsaI GGTCTC 1 cut(s) 787
BsaJI CCNNGG 2 cut(s) 60, 315
BsaWI WCCGGW 2 cut(s) 247, 814
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 286, 333, 427, 861
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 2 cut(s) 338, 1230
Bse3DI GCAATG 1 cut(s) 136
BseAI TCCGGA 1 cut(s) 814
BseDI CCNNGG 2 cut(s) 60, 315
BseGI GGATG 4 cut(s) 121, 220, 747, 1272
BseLI CCNNNNNNNGG 5 cut(s) 66, 286, 333, 427, 861
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 2 cut(s) 338, 1230
BseRI GAGGAG 1 cut(s) 1270
BseSI GKGCMC 3 cut(s) 214, 333, 593
BseXI GCAGC 2 cut(s) 127, 1225
BshFI GGCC 4 cut(s) 49, 135, 326, 1366
BshNI GGYRCC 2 cut(s) 122, 689
BsiHKAI GWGCWC 2 cut(s) 214, 333
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 4 cut(s) 126, 248, 815, 1410
BslFI GGGAC 1 cut(s) 1145
BslI CCNNNNNNNGG 5 cut(s) 66, 286, 333, 427, 861
BsmAI GTCTC 1 cut(s) 787
BsmFI GGGAC 1 cut(s) 1145
BsmI GAATGC 1 cut(s) 1096
BsnI GGCC 4 cut(s) 49, 135, 326, 1366
Bso31I GGTCTC 1 cut(s) 787
BsoBI CYCGRG 1 cut(s) 225
Bsp1286I GDGCHC 3 cut(s) 214, 333, 593
Bsp13I TCCGGA 1 cut(s) 814
Bsp143I GATC 4 cut(s) 407, 601, 817, 1303
BspACI CCGC 1 cut(s) 83
BspANI GGCC 4 cut(s) 49, 135, 326, 1366
BspCNI CTCAG 1 cut(s) 311
BspEI TCCGGA 1 cut(s) 814
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 6 cut(s) 124, 480, 691, 819, 1133, 1134
BspMAI CTGCAG 1 cut(s) 1288
BspPI GGATC 4 cut(s) 415, 812, 825, 1311
BspT107I GGYRCC 2 cut(s) 122, 689
BspTNI GGTCTC 1 cut(s) 787
BsrDI GCAATG 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 2 cut(s) 338, 1230
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 60, 315
BssMI GATC 4 cut(s) 407, 601, 817, 1303
BssT1I CCWWGG 1 cut(s) 60
Bst4CI ACNGT 3 cut(s) 316, 454, 1125
Bst6I CTCTTC 4 cut(s) 200, 936, 1133, 1335
BstC8I GCNNGC 3 cut(s) 24, 81, 1407
BstDEI CTNAG 2 cut(s) 298, 1299
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 250
BstF5I GGATG 4 cut(s) 121, 220, 747, 1272
BstH2I RGCGCY 1 cut(s) 494
BstHHI GCGC 1 cut(s) 493
BstKTI GATC 4 cut(s) 410, 604, 820, 1306
BstMAI GTCTC 1 cut(s) 787
BstMBI GATC 4 cut(s) 407, 601, 817, 1303
BstMWI GCNNNNNNNGC 3 cut(s) 166, 445, 842
BstPI GGTNACC 1 cut(s) 250
BstSFI CTRYAG 2 cut(s) 871, 1284
BstSLI GKGCMC 3 cut(s) 214, 333, 593
BstV1I GCAGC 2 cut(s) 127, 1225
BstV2I GAAGAC 1 cut(s) 1070
BstX2I RGATCY 1 cut(s) 817
BstYI RGATCY 1 cut(s) 817
BsuRI GGCC 4 cut(s) 49, 135, 326, 1366
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 4 cut(s) 121, 220, 747, 1272
BtsIMutI CAGTG 2 cut(s) 331, 1223
Cac8I GCNNGC 3 cut(s) 24, 81, 1407
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 493
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 478, 1132, 1275
Csp6I GTAC 4 cut(s) 70, 670, 690, 1147
CviAII CATG 6 cut(s) 76, 256, 445, 764, 861, 892
CviQI GTAC 4 cut(s) 70, 670, 690, 1147
DdeI CTNAG 2 cut(s) 298, 1299
DpnI GATC 4 cut(s) 409, 603, 819, 1305
DpnII GATC 4 cut(s) 407, 601, 817, 1303
DraIII CACNNNGTG 1 cut(s) 895
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 4 cut(s) 200, 936, 1133, 1335
EarI CTCTTC 4 cut(s) 200, 936, 1133, 1335
Eco130I CCWWGG 1 cut(s) 60
Eco147I AGGCCT 1 cut(s) 1366
Eco31I GGTCTC 1 cut(s) 787
Eco47I GGWCC 3 cut(s) 478, 1132, 1275
Eco57I CTGAAG 2 cut(s) 224, 1227
Eco88I CYCGRG 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 250
EcoO109I RGGNCCY 2 cut(s) 1132, 1275
EcoO65I GGTNACC 1 cut(s) 250
EcoRI GAATTC 2 cut(s) 563, 722
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 6 cut(s) 79, 259, 448, 767, 864, 895
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FaqI GGGAC 1 cut(s) 1145
FatI CATG 6 cut(s) 75, 255, 444, 763, 860, 891
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 3 cut(s) 84, 141, 1214
FokI GGATG 4 cut(s) 128, 207, 734, 1279
Fsp4HI GCNGC 3 cut(s) 84, 141, 1214
FspBI CTAG 1 cut(s) 752
GlaI GCGC 1 cut(s) 492
GluI GCNGC 3 cut(s) 84, 141, 1214
HaeII RGCGCY 1 cut(s) 494
HaeIII GGCC 4 cut(s) 49, 135, 326, 1366
HapII CCGG 4 cut(s) 126, 248, 815, 1410
HhaI GCGC 1 cut(s) 493
Hin1II CATG 6 cut(s) 79, 259, 448, 767, 864, 895
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 3 cut(s) 194, 518, 1345
HpaII CCGG 4 cut(s) 126, 248, 815, 1410
HphI GGTGA 3 cut(s) 262, 409, 1345
Hpy188I TCNGA 5 cut(s) 262, 523, 601, 1207, 1379
Hpy188III TCNNGA 7 cut(s) 367, 500, 764, 794, 815, 959, 980
HpyAV CCTTC 7 cut(s) 421, 1001, 1151, 1202, 1247, 1356, 1399
HpyCH4III ACNGT 3 cut(s) 316, 454, 1125
HpyCH4IV ACGT 2 cut(s) 237, 1371
HpyCH4V TGCA 7 cut(s) 212, 331, 623, 922, 974, 1094, 1286
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 445, 842
HpyF3I CTNAG 2 cut(s) 298, 1299
HpySE526I ACGT 2 cut(s) 237, 1371
Hsp92II CATG 6 cut(s) 79, 259, 448, 767, 864, 895
HspAI GCGC 1 cut(s) 491
KflI GGGWCCC 1 cut(s) 1132
Kpn2I TCCGGA 1 cut(s) 814
KpnI GGTACC 1 cut(s) 693
Kzo9I GATC 4 cut(s) 407, 601, 817, 1303
LpnPI CCDG 9 cut(s) 8, 65, 139, 261, 319, 629, 828, 1211, 1230
Lsp1109I GCAGC 2 cut(s) 127, 1225
LweI GCATC 1 cut(s) 426
MaeI CTAG 1 cut(s) 752
MaeII ACGT 2 cut(s) 237, 1371
MaeIII GTNAC 5 cut(s) 233, 250, 310, 509, 1384
MalI GATC 4 cut(s) 409, 603, 819, 1305
MboI GATC 4 cut(s) 407, 601, 817, 1303
MflI RGATCY 1 cut(s) 817
MhlI GDGCHC 3 cut(s) 214, 333, 593
MlsI TGGCCA 1 cut(s) 135
MluNI TGGCCA 1 cut(s) 135
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 3 cut(s) 241, 456, 1098
Mox20I TGGCCA 1 cut(s) 135
MroI TCCGGA 1 cut(s) 814
MroXI GAANNNNTTC 1 cut(s) 1344
MscI TGGCCA 1 cut(s) 135
MseI TTAA 4 cut(s) 704, 801, 1059, 1271
MslI CAYNNNNRTG 2 cut(s) 248, 470
Msp20I TGGCCA 1 cut(s) 135
MspI CCGG 4 cut(s) 126, 248, 815, 1410
Mva1269I GAATGC 1 cut(s) 1096
MwoI GCNNNNNNNGC 3 cut(s) 166, 445, 842
NdeII GATC 4 cut(s) 407, 601, 817, 1303
NlaIII CATG 6 cut(s) 79, 259, 448, 767, 864, 895
NlaIV GGNNCC 6 cut(s) 124, 480, 691, 819, 1133, 1134
NmuCI GTSAC 3 cut(s) 250, 310, 509
OliI CACNNNNGTG 1 cut(s) 248
PaeR7I CTCGAG 1 cut(s) 225
PagI TCATGA 1 cut(s) 763
PceI AGGCCT 1 cut(s) 1366
PcsI WCGNNNNNNNCGW 1 cut(s) 547
PctI GAATGC 1 cut(s) 1096
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 2 cut(s) 194, 1345
PflFI GACNNNGTC 1 cut(s) 256
PflMI CCANNNNNTGG 1 cut(s) 66
PkrI GCNGC 3 cut(s) 85, 142, 1215
PleI GAGTC 1 cut(s) 512
PpsI GAGTC 1 cut(s) 512
PpuMI RGGWCCY 2 cut(s) 1132, 1275
Psp5II RGGWCCY 2 cut(s) 1132, 1275
PspEI GGTNACC 1 cut(s) 250
PspN4I GGNNCC 6 cut(s) 124, 480, 691, 819, 1133, 1134
PspPI GGNCC 3 cut(s) 478, 1132, 1275
PspPPI RGGWCCY 2 cut(s) 1132, 1275
PspXI VCTCGAGB 1 cut(s) 225
PstI CTGCAG 1 cut(s) 1288
PsuI RGATCY 1 cut(s) 817
PsyI GACNNNGTC 1 cut(s) 256
RsaI GTAC 4 cut(s) 71, 671, 691, 1148
RsaNI GTAC 4 cut(s) 70, 670, 690, 1147
RseI CAYNNNNRTG 2 cut(s) 248, 470
SaqAI TTAA 4 cut(s) 704, 801, 1059, 1271
SatI GCNGC 3 cut(s) 84, 141, 1214
Sau3AI GATC 4 cut(s) 407, 601, 817, 1303
Sau96I GGNCC 3 cut(s) 478, 1132, 1275
ScaI AGTACT 1 cut(s) 1148
SchI GAGTC 1 cut(s) 512
SduI GDGCHC 3 cut(s) 214, 333, 593
SfaNI GCATC 1 cut(s) 426
SfcI CTRYAG 2 cut(s) 871, 1284
Sfr274I CTCGAG 1 cut(s) 225
SinI GGWCC 3 cut(s) 478, 1132, 1275
SlaI CTCGAG 1 cut(s) 225
SmiMI CAYNNNNRTG 2 cut(s) 248, 470
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
SseBI AGGCCT 1 cut(s) 1366
SsiI CCGC 1 cut(s) 83
SspI AATATT 1 cut(s) 716
SspMI CTAG 1 cut(s) 752
StuI AGGCCT 1 cut(s) 1366
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 3 cut(s) 316, 454, 1125
TaiI ACGT 2 cut(s) 240, 1374
TaqI TCGA 5 cut(s) 226, 541, 574, 952, 1005
TatI WGTACW 2 cut(s) 669, 1146
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 2 cut(s) 194, 1345
Tru1I TTAA 4 cut(s) 704, 801, 1059, 1271
Tru9I TTAA 4 cut(s) 704, 801, 1059, 1271
TscAI CASTG 2 cut(s) 338, 1230
TseFI GTSAC 3 cut(s) 250, 310, 509
TseI GCWGC 2 cut(s) 140, 1213
Tsp45I GTSAC 3 cut(s) 250, 310, 509
TspDTI ATGAA 6 cut(s) 433, 576, 735, 752, 780, 1125
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 2 cut(s) 338, 1230
Tth111I GACNNNGTC 1 cut(s) 256
Van91I CCANNNNNTGG 1 cut(s) 66
VneI GTGCAC 2 cut(s) 210, 329
VpaK11BI GGWCC 3 cut(s) 478, 1132, 1275
XapI RAATTY 8 cut(s) 398, 412, 563, 645, 698, 722, 916, 967
XhoI CTCGAG 1 cut(s) 225
XmiI GTMKAC 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 1 cut(s) 752
ZrmI AGTACT 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.