pycom16g25700

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000258
Physical Location & Seq
Forward (+)
27847240 .. 27848858
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g25700.1

Sequence Viewer

Length: 879 bp
ATGGGAAATGCAACTGCATCTGCCGTTGCAGGGAAGGGAAAAGTGACACTTAAACTCACTTCTGGAAAAGAGCTTTCACTCACCAATGTTCTGCATGTTCCTGATATTCTCAAGAACTTGATTTCTGGATTGCTTCTGAGTAACAAAGGTTTCAAAATAGTGTTTGAATCTAATAAGTTTATAATCACCAAGGGTGGGGTGTATGTGGGAAAGAGCTACCTGGCTGAGGGGCTCTTTAAACTGAATGTACTTCCTACTGATGCTATTAATAATAATAACAAACCTAGTTCTTCTTCTGCTTACTTGATTGAGTCCTCTACATTATGTTCTGGTGCACCACATAATTTATGGGGTGAAGCACTCCTTGCTGCAAACACGATTTTGAACCGAATTCCACATAAGAAGACTAATCAATCTCCTTATGAAATCTGGAAAGTCATTCATGTAAATACAATATTTGAATCTGTCGAAGCTGAGTTCTTTGAGGAAAATTTTCCTTATAAAGATAGGTCATATGTTTTGAAAAAAAGAGTACATGATGATACTACAAGAAATGATGACAATAATCATGCTTCAACTTTTAGAGTTCAAAGTCAAGATTTGGAACCATGGAAGAGTAAAAGAGCAATAATTGCAAAGAATTTGGGTCCTGATTTCCTTACTTTTGTAATTGAAGAAAAGCCTCAAACTTATAAGGCTGCATTAGAATCATCCGAAGCATCTTATTGGAAAGAAGCTATTCAAAGTGAAATGGAATCCATCATGCAAAATAATACATGGGAATTGATTAATTTGCCTCCTGGAAATAAACCAATACGACACAAATGGATTTTTAAGAGGAAATTGAGACTGGGTGGTGCCATAGACAAAAGGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

32.82

Weight (kDa)

9.39

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 1 - 51 1.1e-10 Pol polyprotein, beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000258)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00980
fragaria_vesca FvH4_1g25682 FvH4_2g13961 FvH4_2g20162 FvH4_2g24753 FvH4_3g12191 FvH4_3g12632 FvH4_3g16171 FvH4_4g05871 FvH4_4g05873 FvH4_4g10121 FvH4_4g15488 FvH4_4g23911 FvH4_4g27782 FvH4_5g08912 FvH4_5g23001 FvH4_5g24552 FvH4_5g32801 FvH4_5g32802 FvH4_6g05093 FvH4_6g19722 FvH4_6g20391 FvH4_6g33792 FvH4_7g01381 FvH4_7g16761 FvH4_7g16761 FvH4_7g16761
malus_domestica MD01G1082500.v1.1 MD15G1436400.v1.1
prunus_persica Prupe.2G021400_v2.0.a1 Prupe.2G052400_v2.0.a1 Prupe.2G065000_v2.0.a1 Prupe.2G187700_v2.0.a1 Prupe.2G187800_v2.0.a1 Prupe.2G189900_v2.0.a1 Prupe.2G191300_v2.0.a1 Prupe.2G191700_v2.0.a1 Prupe.4G265600_v2.0.a1 Prupe.7G014200_v2.0.a1 Prupe.7G039000_v2.0.a1
pyrus_communis pycom01g05590 pycom01g05910 pycom02g12230 pycom02g25840 pycom03g15580 pycom04g04480 pycom05g09230 pycom05g23350 pycom05g23360 pycom07g08380 pycom09g01980 pycom09g01990 pycom11g18190 pycom11g18200 pycom11g21580 pycom13g04160 pycom15g25800 pycom16g16210 pycom16g17230 pycom16g24560 pycom16g25700 pycom17g11020
rosa_chinensis RchiOBHm_Chr1g0359981 RchiOBHm_Chr2g0151961 RchiOBHm_Chr2g0171331 RchiOBHm_Chr5g0081471 RchiOBHm_Chr6g0283031 RchiOBHm_Chr7g0197111 RchiOBHm_Chr7g0222601
rosa_laevigata RLG00000027843 RLG00000027845 RLG00000030065
rosa_multiflora Rmu_sc0000381.1_g000031 Rmu_sc0001179.1_g000035 Rmu_sc0001616.1_g000046 Rmu_sc0001851.1_g000032 Rmu_sc0002152.1_g000013 Rmu_sc0004031.1_g000015 Rmu_sc0004645.1_g000003 Rmu_sc0007393.1_g000001 Rmu_sc0008949.1_g000003 Rmu_sc0009799.1_g000011 Rmu_sc0011614.1_g000001 Rmu_sc0011699.1_g000009 Rmu_sc0019280.1_g000001 Rmu_sc0023984.1_g000001 Rmu_ssc0000050.1_g000036
rosa_roxburghii Rroxscaffold_1G00022650 Rroxscaffold_1G00033480 Rroxscaffold_3G00270250 Rroxscaffold_4G00296600 Rroxscaffold_4G00296760 Rroxscaffold_6G00409840
rosa_rugosa Rorug01G0273800 Rorug01G0273900 Rorug01G0274000
rosa_samantha Rh1AG287300 Rh1AG288100 Rh1BG253000 Rh1BG253500 Rh1BG399800 Rh1CG270400 Rh1CG271000 Rh1DG282000 Rh1DG282500 Rh4DG126500 Rh5DG194600 Rh5DG461100
rosa_wichuraiana Rw0G009130 Rw1G002590 Rw1G008490 Rw1G012210 Rw1G014290 Rw1G014490 Rw1G025510 Rw2G025840 Rw2G026170 Rw2G036510 Rw3G003050 Rw4G009270 Rw5G002350 Rw5G013930 Rw5G019500 Rw5G025890 Rw5G029960 Rw5G032360 Rw6G009890 Rw6G015670 Rw6G015860 Rw7G010030 Rw7G032600 Rw7G032770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 182, 501, 693
AccB1I GGYRCC 1 cut(s) 857
AcsI RAATTY 3 cut(s) 390, 490, 640
AfaI GTAC 2 cut(s) 249, 534
AfiI CCNNNNNNNGG 3 cut(s) 30, 195, 226
AgsI TTSAA 9 cut(s) 154, 167, 385, 461, 523, 576, 590, 674, 743
AjnI CCWGG 2 cut(s) 219, 799
AluBI AGCT 4 cut(s) 73, 216, 473, 737
AluI AGCT 4 cut(s) 73, 216, 473, 737
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 841
Alw44I GTGCAC 1 cut(s) 333
ApaLI GTGCAC 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 368, 698
ApoI RAATTY 3 cut(s) 390, 490, 640
AseI ATTAAT 2 cut(s) 267, 789
Asp700I GAANNNNTTC 2 cut(s) 492, 738
AspS9I GGNCC 1 cut(s) 647
AsuHPI GGTGA 3 cut(s) 73, 178, 365
AvaII GGWCC 1 cut(s) 647
BaeGI GKGCMC 1 cut(s) 337
BanI GGYRCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 337
BbvCI CCTCAGC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 355, 685
BccI CCATC 1 cut(s) 767
BceAI ACGGC 1 cut(s) 8
BciT130I CCWGG 2 cut(s) 221, 801
BcoDI GTCTC 1 cut(s) 841
BfaI CTAG 1 cut(s) 285
BisI GCNGC 2 cut(s) 369, 699
BlsI GCNGC 2 cut(s) 370, 700
Bme1390I CCNGG 2 cut(s) 221, 801
Bme18I GGWCC 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 3 cut(s) 606, 648, 859
BmrFI CCNGG 2 cut(s) 221, 801
BmrI ACTGGG 1 cut(s) 860
BmsI GCATC 3 cut(s) 26, 250, 728
BmuI ACTGGG 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 189, 608
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 195, 226
Bse1I ACTGG 1 cut(s) 855
BseBI CCWGG 2 cut(s) 221, 801
BseDI CCNNGG 2 cut(s) 189, 608
BseGI GGATG 1 cut(s) 710
BseLI CCNNNNNNNGG 3 cut(s) 30, 195, 226
BseMII CTCAG 3 cut(s) 128, 216, 465
BseNI ACTGG 1 cut(s) 855
BseSI GKGCMC 1 cut(s) 337
BseXI GCAGC 2 cut(s) 355, 685
BshNI GGYRCC 1 cut(s) 857
BsiHKAI GWGCWC 1 cut(s) 337
BslI CCNNNNNNNGG 3 cut(s) 30, 195, 226
BsmAI GTCTC 1 cut(s) 841
Bsp1286I GDGCHC 2 cut(s) 234, 337
Bsp19I CCATGG 1 cut(s) 608
BspCNI CTCAG 3 cut(s) 129, 217, 466
BspLI GGNNCC 3 cut(s) 606, 648, 859
BspT107I GGYRCC 1 cut(s) 857
BsrI ACTGG 1 cut(s) 855
BssECI CCNNGG 2 cut(s) 189, 608
BssT1I CCWWGG 2 cut(s) 189, 608
Bst2UI CCWGG 2 cut(s) 221, 801
Bst6I CTCTTC 1 cut(s) 608
BstAPI GCANNNNNTGC 2 cut(s) 365, 632
BstDEI CTNAG 3 cut(s) 137, 225, 474
BstDSI CCRYGG 1 cut(s) 608
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 710
BstMAI GTCTC 1 cut(s) 841
BstMWI GCNNNNNNNGC 2 cut(s) 365, 632
BstNI CCWGG 2 cut(s) 221, 801
BstNSI RCATGY 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 219, 799
BstSLI GKGCMC 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 355, 685
BstV2I GAAGAC 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 608
BtsCI GGATG 1 cut(s) 710
Cfr13I GGNCC 1 cut(s) 647
Csp6I GTAC 2 cut(s) 248, 533
CviAII CATG 7 cut(s) 95, 443, 536, 569, 609, 763, 777
CviJI RGCY 8 cut(s) 73, 216, 224, 232, 473, 682, 698, 737
CviKI_1 RGCY 8 cut(s) 73, 216, 224, 232, 473, 682, 698, 737
CviQI GTAC 2 cut(s) 248, 533
DdeI CTNAG 3 cut(s) 137, 225, 474
DraI TTTAAA 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
Eco130I CCWWGG 2 cut(s) 189, 608
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 1 cut(s) 647
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 647
EcoRI GAATTC 1 cut(s) 390
EcoRII CCWGG 2 cut(s) 219, 799
EcoT14I CCWWGG 2 cut(s) 189, 608
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 2 cut(s) 189, 608
FaeI CATG 7 cut(s) 98, 446, 539, 572, 612, 766, 780
FalI AAGNNNNNCTT 4 cut(s) 33, 65, 348, 380
FatI CATG 7 cut(s) 94, 442, 535, 568, 608, 762, 776
FauNDI CATATG 1 cut(s) 514
Fnu4HI GCNGC 2 cut(s) 369, 699
FokI GGATG 1 cut(s) 697
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 2 cut(s) 369, 699
FspBI CTAG 1 cut(s) 285
GluI GCNGC 2 cut(s) 369, 699
Hin1II CATG 7 cut(s) 98, 446, 539, 572, 612, 766, 780
HinfI GANTC 5 cut(s) 167, 311, 461, 707, 755
HphI GGTGA 3 cut(s) 73, 178, 365
Hpy166II GTNNAC 1 cut(s) 335
Hpy188I TCNGA 2 cut(s) 138, 715
Hpy188III TCNNGA 7 cut(s) 63, 101, 112, 126, 430, 596, 650
Hpy8I GTNNAC 1 cut(s) 335
HpyAV CCTTC 1 cut(s) 28
HpyCH4V TGCA 9 cut(s) 11, 17, 29, 94, 335, 371, 635, 701, 766
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 632
HpyF3I CTNAG 3 cut(s) 137, 225, 474
Hsp92II CATG 7 cut(s) 98, 446, 539, 572, 612, 766, 780
Lsp1109I GCAGC 2 cut(s) 355, 685
LweI GCATC 3 cut(s) 26, 250, 728
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 2 cut(s) 43, 140
MboII GAAGA 5 cut(s) 282, 285, 415, 625, 686
MhlI GDGCHC 2 cut(s) 234, 337
MluCI AATT 9 cut(s) 343, 390, 490, 630, 640, 669, 782, 790, 842
MlyI GAGTC 1 cut(s) 320
MnlI CCTC 6 cut(s) 220, 325, 478, 693, 807, 831
MroXI GAANNNNTTC 2 cut(s) 492, 738
MseI TTAA 5 cut(s) 51, 237, 267, 789, 834
MspR9I CCNGG 2 cut(s) 221, 801
MvaI CCWGG 2 cut(s) 221, 801
MwoI GCNNNNNNNGC 2 cut(s) 365, 632
NcoI CCATGG 1 cut(s) 608
NdeI CATATG 1 cut(s) 514
NlaIII CATG 7 cut(s) 98, 446, 539, 572, 612, 766, 780
NlaIV GGNNCC 3 cut(s) 606, 648, 859
NmuCI GTSAC 1 cut(s) 43
NspI RCATGY 1 cut(s) 98
PdmI GAANNNNTTC 2 cut(s) 492, 738
PfeI GAWTC 4 cut(s) 167, 461, 707, 755
PfoI TCCNGGA 1 cut(s) 799
PkrI GCNGC 2 cut(s) 370, 700
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
PpuMI RGGWCCY 1 cut(s) 647
PshBI ATTAAT 2 cut(s) 267, 789
PsiI TTATAA 3 cut(s) 182, 501, 693
Psp5II RGGWCCY 1 cut(s) 647
Psp6I CCWGG 2 cut(s) 219, 799
PspGI CCWGG 2 cut(s) 219, 799
PspN4I GGNNCC 3 cut(s) 606, 648, 859
PspPI GGNCC 1 cut(s) 647
PspPPI RGGWCCY 1 cut(s) 647
RsaI GTAC 2 cut(s) 249, 534
RsaNI GTAC 2 cut(s) 248, 533
SaqAI TTAA 5 cut(s) 51, 237, 267, 789, 834
SatI GCNGC 2 cut(s) 369, 699
Sau96I GGNCC 1 cut(s) 647
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 2 cut(s) 221, 801
SduI GDGCHC 2 cut(s) 234, 337
SetI ASST 8 cut(s) 75, 151, 218, 222, 286, 475, 512, 739
SfaNI GCATC 3 cut(s) 26, 250, 728
SinI GGWCC 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 9 cut(s) 343, 390, 490, 630, 640, 669, 782, 790, 842
SspI AATATT 1 cut(s) 456
SspMI CTAG 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 219, 799
StyI CCWWGG 2 cut(s) 189, 608
TaqI TCGA 1 cut(s) 468
TasI AATT 9 cut(s) 343, 390, 490, 630, 640, 669, 782, 790, 842
TatI WGTACW 2 cut(s) 247, 532
TfiI GAWTC 4 cut(s) 167, 461, 707, 755
Tru1I TTAA 5 cut(s) 51, 237, 267, 789, 834
Tru9I TTAA 5 cut(s) 51, 237, 267, 789, 834
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 2 cut(s) 368, 698
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 431, 438
VneI GTGCAC 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 647
VspI ATTAAT 2 cut(s) 267, 789
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 3 cut(s) 390, 490, 640
XceI RCATGY 1 cut(s) 98
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmnI GAANNNNTTC 2 cut(s) 492, 738
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.