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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 149 |
| AflII | CTTAAG | 1 cut(s) | 52 |
| AgsI | TTSAA | 1 cut(s) | 97 |
| ApoI | RAATTY | 1 cut(s) | 149 |
| BclI | TGATCA | 1 cut(s) | 133 |
| BfaI | CTAG | 1 cut(s) | 128 |
| BfrI | CTTAAG | 1 cut(s) | 52 |
| BmiI | GGNNCC | 1 cut(s) | 76 |
| BseRI | GAGGAG | 1 cut(s) | 23 |
| Bsp143I | GATC | 1 cut(s) | 133 |
| BspLI | GGNNCC | 1 cut(s) | 76 |
| BspTI | CTTAAG | 1 cut(s) | 52 |
| BssMI | GATC | 1 cut(s) | 133 |
| Bst4CI | ACNGT | 1 cut(s) | 19 |
| BstAFI | CTTAAG | 1 cut(s) | 52 |
| BstDEI | CTNAG | 1 cut(s) | 154 |
| BstKTI | GATC | 1 cut(s) | 136 |
| BstMBI | GATC | 1 cut(s) | 133 |
| CviAII | CATG | 1 cut(s) | 35 |
| DdeI | CTNAG | 1 cut(s) | 154 |
| DpnI | GATC | 1 cut(s) | 135 |
| DpnII | GATC | 1 cut(s) | 133 |
| DraI | TTTAAA | 1 cut(s) | 43 |
| FaeI | CATG | 1 cut(s) | 38 |
| FaiI | YATR | 2 cut(s) | 36, 113 |
| FatI | CATG | 1 cut(s) | 34 |
| FbaI | TGATCA | 1 cut(s) | 133 |
| FspBI | CTAG | 1 cut(s) | 128 |
| Hin1II | CATG | 1 cut(s) | 38 |
| HinfI | GANTC | 1 cut(s) | 65 |
| Hpy166II | GTNNAC | 1 cut(s) | 84 |
| Hpy8I | GTNNAC | 1 cut(s) | 84 |
| HpyCH4III | ACNGT | 1 cut(s) | 19 |
| HpyCH4V | TGCA | 1 cut(s) | 28 |
| HpyF3I | CTNAG | 1 cut(s) | 154 |
| Hsp92II | CATG | 1 cut(s) | 38 |
| Ksp22I | TGATCA | 1 cut(s) | 133 |
| Kzo9I | GATC | 1 cut(s) | 133 |
| MaeI | CTAG | 1 cut(s) | 128 |
| MalI | GATC | 1 cut(s) | 135 |
| MboI | GATC | 1 cut(s) | 133 |
| MluCI | AATT | 2 cut(s) | 92, 149 |
| MlyI | GAGTC | 1 cut(s) | 74 |
| MnlI | CCTC | 2 cut(s) | 59, 119 |
| MseI | TTAA | 2 cut(s) | 42, 53 |
| MspCI | CTTAAG | 1 cut(s) | 52 |
| NdeII | GATC | 1 cut(s) | 133 |
| NlaIII | CATG | 1 cut(s) | 38 |
| NlaIV | GGNNCC | 1 cut(s) | 76 |
| PleI | GAGTC | 1 cut(s) | 73 |
| PpsI | GAGTC | 1 cut(s) | 73 |
| PspN4I | GGNNCC | 1 cut(s) | 76 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 67, 99 |
| SaqAI | TTAA | 2 cut(s) | 42, 53 |
| Sau3AI | GATC | 1 cut(s) | 133 |
| SchI | GAGTC | 1 cut(s) | 74 |
| SetI | ASST | 1 cut(s) | 51 |
| SgeI | CNNG | 5 cut(s) | 31, 47, 91, 117, 140 |
| SmlI | CTYRAG | 1 cut(s) | 52 |
| SmoI | CTYRAG | 1 cut(s) | 52 |
| Sse9I | AATT | 2 cut(s) | 92, 149 |
| SspMI | CTAG | 1 cut(s) | 128 |
| TaaI | ACNGT | 1 cut(s) | 19 |
| TasI | AATT | 2 cut(s) | 92, 149 |
| Tru1I | TTAA | 2 cut(s) | 42, 53 |
| Tru9I | TTAA | 2 cut(s) | 42, 53 |
| Vha464I | CTTAAG | 1 cut(s) | 52 |
| XapI | RAATTY | 1 cut(s) | 149 |
| XspI | CTAG | 1 cut(s) | 128 |