Prupe.5G127600_v2.0.a1
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000780
Physical Location & Seq
Forward (+)
12449563 .. 12450126
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G127600.1

Sequence Viewer

Length: 564 bp
ATGGGGCATGACCGCAGTGAAGGGAATGGTGTGGAAGGGGCTACTCTAGGAACATCGTTTGGGGTGCTCTTTGGTGCTGTTATACAAGTGAAGGACAAGACTAAGATGTTTAAGTGCATCCTCGAGTATCTCGAATCCACGATAGACTCTCTAAAACCATTTATAGAAGAGATGGTAGAATATAACAAGGTATTGCATCTCTCAAAAGAGGAACTAGGGAATTTTATAACACTTATGGAGAAGGGTGCACAGCTCATTCACAAGTGCTCAAAGATTCGTAAGTGGGCTAGCTACAAGAAGTATGAATATGCCAACAAACTTCTTAGATTAGATGAATCTCTTCAAGTAATGTTAAATATATTTAGAGTGCAATTAGTAAGGGATGTGAGAGAGAGCTTAGTTTCCTTAAGTAATATAGAGGCTATGATCAAGCGAATGGAAGGGAGTGATTTGATACAAAATGATCAAATTTTAAGTATAGGTGGTTCTGCAGTGCCTGAAGTTTACCTCCATCGGCCTGGATGTATTGAATGTACATGGAACACAAGATGTGCAGTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.22

Weight (kDa)

6.9

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AciI CCGC 1 cut(s) 13
AcsI RAATTY 2 cut(s) 220, 468
AcuI CTGAAG 1 cut(s) 519
AfaI GTAC 1 cut(s) 535
AflII CTTAAG 1 cut(s) 406
AgsI TTSAA 2 cut(s) 344, 530
AjnI CCWGG 1 cut(s) 517
AluBI AGCT 3 cut(s) 253, 291, 396
AluI AGCT 3 cut(s) 253, 291, 396
Alw21I GWGCWC 3 cut(s) 69, 250, 269
Alw44I GTGCAC 1 cut(s) 246
AlwNI CAGNNNCTG 1 cut(s) 497
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 1 cut(s) 515
ApaLI GTGCAC 1 cut(s) 246
ApoI RAATTY 2 cut(s) 220, 468
Asp700I GAANNNNTTC 1 cut(s) 339
AsuNHI GCTAGC 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 250
Bbv12I GWGCWC 3 cut(s) 69, 250, 269
BccI CCATC 2 cut(s) 166, 519
BciT130I CCWGG 1 cut(s) 519
BclI TGATCA 2 cut(s) 426, 463
BfaI CTAG 3 cut(s) 47, 215, 288
BfmI CTRYAG 1 cut(s) 489
BfrI CTTAAG 1 cut(s) 406
Bme1390I CCNGG 1 cut(s) 519
BmeT110I CYCGRG 1 cut(s) 122
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 2 cut(s) 126, 205
BmtI GCTAGC 1 cut(s) 291
BseBI CCWGG 1 cut(s) 519
BseGI GGATG 3 cut(s) 117, 388, 527
BseSI GKGCMC 1 cut(s) 250
BshFI GGCC 1 cut(s) 517
BsiHKAI GWGCWC 3 cut(s) 69, 250, 269
BsiHKCI CYCGRG 1 cut(s) 122
BsnI GGCC 1 cut(s) 517
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 69, 250, 269
Bsp1407I TGTACA 1 cut(s) 533
Bsp143I GATC 2 cut(s) 426, 463
BspACI CCGC 1 cut(s) 13
BspANI GGCC 1 cut(s) 517
BspMAI CTGCAG 1 cut(s) 493
BspOI GCTAGC 1 cut(s) 291
BspTI CTTAAG 1 cut(s) 406
BsrGI TGTACA 1 cut(s) 533
BssMI GATC 2 cut(s) 426, 463
Bst2UI CCWGG 1 cut(s) 519
Bst6I CTCTTC 2 cut(s) 162, 345
BstAFI CTTAAG 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 533
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 102, 323, 397
BstF5I GGATG 3 cut(s) 117, 388, 527
BstKTI GATC 2 cut(s) 429, 466
BstMBI GATC 2 cut(s) 426, 463
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 517
BstSFI CTRYAG 1 cut(s) 489
BstSLI GKGCMC 1 cut(s) 250
BstXI CCANNNNNNTGG 1 cut(s) 518
BsuRI GGCC 1 cut(s) 517
BtsCI GGATG 3 cut(s) 117, 388, 527
BtsI GCAGTG 3 cut(s) 22, 498, 561
BtsIMutI CAGTG 3 cut(s) 22, 498, 561
Cac8I GCNNGC 1 cut(s) 289
CaiI CAGNNNCTG 1 cut(s) 497
Csp6I GTAC 1 cut(s) 534
CviAII CATG 2 cut(s) 8, 537
CviJI RGCY 7 cut(s) 41, 253, 287, 291, 396, 422, 517
CviKI_1 RGCY 7 cut(s) 41, 253, 287, 291, 396, 422, 517
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 3 cut(s) 102, 323, 397
DpnI GATC 2 cut(s) 428, 465
DpnII GATC 2 cut(s) 426, 463
Eam1104I CTCTTC 2 cut(s) 162, 345
EarI CTCTTC 2 cut(s) 162, 345
Eco57I CTGAAG 1 cut(s) 519
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 517
FaeI CATG 2 cut(s) 11, 540
FatI CATG 2 cut(s) 7, 536
FbaI TGATCA 2 cut(s) 426, 463
FokI GGATG 3 cut(s) 104, 395, 534
FspBI CTAG 3 cut(s) 47, 215, 288
HaeIII GGCC 1 cut(s) 517
Hin1II CATG 2 cut(s) 11, 540
HinfI GANTC 4 cut(s) 134, 146, 274, 335
Hpy166II GTNNAC 2 cut(s) 248, 505
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 2 cut(s) 248, 505
HpyAV CCTTC 5 cut(s) 14, 29, 85, 235, 434
HpyCH4V TGCA 6 cut(s) 117, 196, 248, 370, 491, 554
HpyF3I CTNAG 3 cut(s) 102, 323, 397
Hsp92II CATG 2 cut(s) 11, 540
Ksp22I TGATCA 2 cut(s) 426, 463
Kzo9I GATC 2 cut(s) 426, 463
LpnPI CCDG 3 cut(s) 504, 510, 531
LweI GCATC 2 cut(s) 126, 205
MaeI CTAG 3 cut(s) 47, 215, 288
MalI GATC 2 cut(s) 428, 465
MboI GATC 2 cut(s) 426, 463
MboII GAAGA 2 cut(s) 179, 332
MhlI GDGCHC 3 cut(s) 69, 250, 269
MluCI AATT 3 cut(s) 220, 371, 468
MlyI GAGTC 1 cut(s) 140
MnlI CCTC 4 cut(s) 131, 202, 412, 518
MroXI GAANNNNTTC 1 cut(s) 339
MseI TTAA 5 cut(s) 111, 353, 407, 473, 562
MspCI CTTAAG 1 cut(s) 406
MspR9I CCNGG 1 cut(s) 519
MvaI CCWGG 1 cut(s) 519
NdeII GATC 2 cut(s) 426, 463
NheI GCTAGC 1 cut(s) 287
NlaIII CATG 2 cut(s) 11, 540
PaeR7I CTCGAG 1 cut(s) 122
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 3 cut(s) 134, 274, 335
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PsiI TTATAA 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspXI VCTCGAGB 1 cut(s) 122
PsrI GAACNNNNNNTAC 2 cut(s) 469, 501
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
SaqAI TTAA 5 cut(s) 111, 353, 407, 473, 562
Sau3AI GATC 2 cut(s) 426, 463
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 519
SduI GDGCHC 3 cut(s) 69, 250, 269
SetI ASST 6 cut(s) 192, 255, 293, 398, 484, 510
SfaNI GCATC 2 cut(s) 126, 205
SfcI CTRYAG 1 cut(s) 489
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 122, 406
SmoI CTYRAG 2 cut(s) 122, 406
Sse9I AATT 3 cut(s) 220, 371, 468
SsiI CCGC 1 cut(s) 13
SspMI CTAG 3 cut(s) 47, 215, 288
StyD4I CCNGG 1 cut(s) 517
TaqI TCGA 2 cut(s) 123, 132
TasI AATT 3 cut(s) 220, 371, 468
TatI WGTACW 1 cut(s) 533
TfiI GAWTC 3 cut(s) 134, 274, 335
Tru1I TTAA 5 cut(s) 111, 353, 407, 473, 562
Tru9I TTAA 5 cut(s) 111, 353, 407, 473, 562
TscAI CASTG 3 cut(s) 22, 498, 561
TspDTI ATGAA 2 cut(s) 318, 348
TspRI CASTG 3 cut(s) 22, 498, 561
Vha464I CTTAAG 1 cut(s) 406
VneI GTGCAC 1 cut(s) 246
XapI RAATTY 2 cut(s) 220, 468
XhoI CTCGAG 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 3 cut(s) 47, 215, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.