Rh2DG454500

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000780
Physical Location & Seq
Forward (+)
65787860 .. 65789202
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG454500.1

Sequence Viewer

Length: 861 bp
ATGTCACCAAGAAAGCAAGAGAAGTGCACTGAATCTTCCATACTCTTCTGCTTTCTCTTATCTTCAACTCTTCTGGTTTTGGTGCACACATATTCAAATCCAATATCGAAGGGAATGGCCGGGGAGTTTGTCGGAGGGGCTGCTCTGGGAACAGCATTCAGTGCACTGTACGACGGCGTTAAAGAGCTGATTTCCAAGAATCACATATTCAAATCCCTCCTCGTAAACATGAAATCCAGTCTTGAGACTTTAAAACCGCTTATCCAACAAATAGCGCAGTCTAACGAGCTCCTGTCTCGCCCAGAGGAGGAGCTGAAAGGCTTGGAAGCAGTAATGGAGGATGGCAAAGATCTTATCAGCAAGTGCTCGAAGATTCGGCAATGGAACATCTACAAGAAGTACGAGTACGCCAACAAACTTGTCGAGTGGGATCAGTTGCTTCGGAGACAGTTGGATGTACTGAAGGTGCAAGAGGCGAGGGATGTGAAGGAGAACACGGTTTTGTTGAGGACTATAGAGGCGGTGGTCAAACGAATCGAAGAGAATGTTGTGGTAAGAAATTATAGCTCTACAGTTGAACCTCGTGATCTGGAGAGACGTGTTGTTGAGGATCCAGCTAATAAGAGTTTTGGTTCCGAAGAAGACGACGGAGAGGTTTGTTCTCGAAAGACAGAAGTATATGGCACTGCACTTTACAACTTCACTGCCGGTGGAGAGGATGAGTTGAATTTGATTGCTGGAGATGAAATTGAAATTGAGTATGAAGTCGACGACTGGTTTTATGTGAAGAAGAAACGCCCTGGGAAGGATGGGAAAATGGCTGGTCTAGTCCCGGTCCTCTATGTGAGATATTTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

286

Amino Acids

32.46

Weight (kDa)

5.33

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 42 - 171 1.5e-29 Arabidopsis broad-spectrum mildew resistance protein RPW8
SH3_1 PF00018 229 - 271 7.5e-06 SH3 domain
SH3_9 PF14604 230 - 282 4.5e-06 Variant SH3 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 768
AciI CCGC 2 cut(s) 257, 521
AclWI GGATC 3 cut(s) 438, 605, 618
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 1 cut(s) 727
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 4 cut(s) 170, 401, 407, 459
AfiI CCNNNNNNNGG 2 cut(s) 307, 805
AflIII ACRYGT 1 cut(s) 598
AgsI TTSAA 6 cut(s) 66, 96, 211, 578, 727, 752
AjiI CACGTC 1 cut(s) 599
AjnI CCWGG 1 cut(s) 799
AluBI AGCT 5 cut(s) 187, 289, 313, 567, 617
AluI AGCT 5 cut(s) 187, 289, 313, 567, 617
Alw21I GWGCWC 5 cut(s) 29, 87, 166, 291, 368
Alw26I GTCTC 4 cut(s) 239, 300, 439, 589
Alw44I GTGCAC 3 cut(s) 25, 83, 162
AlwI GGATC 3 cut(s) 438, 605, 618
AoxI GGCC 1 cut(s) 117
ApaLI GTGCAC 3 cut(s) 25, 83, 162
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 727
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 835
AsuC2I CCSGG 2 cut(s) 121, 833
AvaII GGWCC 1 cut(s) 835
BaeGI GKGCMC 3 cut(s) 29, 87, 166
BamHI GGATCC 1 cut(s) 610
BanII GRGCYC 1 cut(s) 291
BarI GAAGNNNNNNTAC 2 cut(s) 389, 421
BauI CACGAG 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 648
Bbv12I GWGCWC 5 cut(s) 29, 87, 166, 291, 368
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 335, 803
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 801
BcnI CCSGG 2 cut(s) 121, 833
BcoDI GTCTC 4 cut(s) 239, 300, 439, 589
BfaI CTAG 1 cut(s) 827
BfmI CTRYAG 2 cut(s) 513, 570
BglII AGATCT 1 cut(s) 349
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 3 cut(s) 121, 801, 833
Bme18I GGWCC 1 cut(s) 835
BmgBI CACGTC 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 835
BmiI GGNNCC 2 cut(s) 612, 634
BmrFI CCNGG 3 cut(s) 121, 801, 833
BpiI GAAGAC 1 cut(s) 648
BpmI CTGGAG 2 cut(s) 611, 759
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 121, 833
BsaJI CCNNGG 3 cut(s) 120, 799, 800
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 805
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 2 cut(s) 237, 779
Bse3DI GCAATG 1 cut(s) 386
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 3 cut(s) 120, 799, 800
BseGI GGATG 5 cut(s) 346, 460, 487, 724, 814
BseLI CCNNNNNNNGG 2 cut(s) 307, 805
BseMI GCAATG 1 cut(s) 386
BseNI ACTGG 2 cut(s) 237, 779
BseRI GAGGAG 3 cut(s) 209, 320, 323
BseSI GKGCMC 3 cut(s) 29, 87, 166
BseXI GCAGC 1 cut(s) 127
BsgI GTGCAG 1 cut(s) 672
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 5 cut(s) 29, 87, 166, 291, 368
BsiSI CCGG 3 cut(s) 120, 708, 833
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 2 cut(s) 307, 805
BsmAI GTCTC 4 cut(s) 239, 300, 439, 589
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 815
BsmI GAATGC 1 cut(s) 155
BsnI GGCC 1 cut(s) 119
Bsp1286I GDGCHC 5 cut(s) 29, 87, 166, 291, 368
Bsp143I GATC 4 cut(s) 349, 430, 586, 610
BspACI CCGC 2 cut(s) 257, 521
BspANI GGCC 1 cut(s) 119
BspLI GGNNCC 2 cut(s) 612, 634
BspPI GGATC 3 cut(s) 438, 605, 618
BsrDI GCAATG 1 cut(s) 386
BsrFI RCCGGY 1 cut(s) 707
BsrI ACTGG 2 cut(s) 237, 779
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 3 cut(s) 120, 799, 800
BssMI GATC 4 cut(s) 349, 430, 586, 610
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 4 cut(s) 168, 450, 499, 574
Bst6I CTCTTC 3 cut(s) 50, 75, 534
BstAPI GCANNNNNTGC 1 cut(s) 161
BstF5I GGATG 5 cut(s) 346, 460, 487, 724, 814
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 4 cut(s) 352, 433, 589, 613
BstMAI GTCTC 4 cut(s) 239, 300, 439, 589
BstMBI GATC 4 cut(s) 349, 430, 586, 610
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 1 cut(s) 801
BstSCI CCNGG 3 cut(s) 119, 799, 831
BstSFI CTRYAG 2 cut(s) 513, 570
BstSLI GKGCMC 3 cut(s) 29, 87, 166
BstV1I GCAGC 1 cut(s) 127
BstV2I GAAGAC 1 cut(s) 648
BstX2I RGATCY 2 cut(s) 349, 610
BstYI RGATCY 2 cut(s) 349, 610
BsuRI GGCC 1 cut(s) 119
BtrI CACGTC 1 cut(s) 599
BtsCI GGATG 5 cut(s) 346, 460, 487, 724, 814
BtsI GCAGTG 2 cut(s) 684, 702
BtsIMutI CAGTG 5 cut(s) 27, 164, 166, 684, 702
CfoI GCGC 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 707
Cfr13I GGNCC 1 cut(s) 835
Csp6I GTAC 4 cut(s) 169, 400, 406, 458
CviAII CATG 1 cut(s) 229
CviJI RGCY 9 cut(s) 119, 140, 187, 289, 313, 321, 567, 617, 821
CviKI_1 RGCY 9 cut(s) 119, 140, 187, 289, 313, 321, 567, 617, 821
CviQI GTAC 4 cut(s) 169, 400, 406, 458
DpnI GATC 4 cut(s) 351, 432, 588, 612
DpnII GATC 4 cut(s) 349, 430, 586, 610
DraI TTTAAA 1 cut(s) 252
EaeI YGGCCR 1 cut(s) 117
Eam1104I CTCTTC 3 cut(s) 50, 75, 534
EarI CTCTTC 3 cut(s) 50, 75, 534
Ecl136II GAGCTC 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 835
Eco53kI GAGCTC 1 cut(s) 289
Eco57I CTGAAG 1 cut(s) 482
EcoICRI GAGCTC 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 799
EcoT38I GRGCYC 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 1 cut(s) 232
FaqI GGGAC 1 cut(s) 815
FatI CATG 1 cut(s) 228
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 5 cut(s) 353, 467, 494, 731, 821
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 827
GlaI GCGC 1 cut(s) 276
GluI GCNGC 1 cut(s) 141
GsuI CTGGAG 2 cut(s) 611, 759
HaeIII GGCC 1 cut(s) 119
HapII CCGG 3 cut(s) 120, 708, 833
HhaI GCGC 1 cut(s) 277
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 4 cut(s) 32, 199, 373, 534
HpaII CCGG 3 cut(s) 120, 708, 833
Hpy166II GTNNAC 5 cut(s) 27, 85, 164, 226, 769
Hpy188I TCNGA 3 cut(s) 134, 444, 637
Hpy188III TCNNGA 5 cut(s) 242, 584, 590, 663, 858
Hpy8I GTNNAC 5 cut(s) 27, 85, 164, 226, 769
Hpy99I CGWCG 3 cut(s) 176, 650, 773
HpyAV CCTTC 4 cut(s) 103, 457, 481, 799
HpyCH4III ACNGT 4 cut(s) 168, 450, 499, 574
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 5 cut(s) 27, 85, 164, 469, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 598
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 1 cut(s) 275
Kzo9I GATC 4 cut(s) 349, 430, 586, 610
LmnI GCTCC 2 cut(s) 294, 310
Lsp1109I GCAGC 1 cut(s) 127
MaeI CTAG 1 cut(s) 827
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 1 cut(s) 3
MalI GATC 4 cut(s) 351, 432, 588, 612
MboI GATC 4 cut(s) 349, 430, 586, 610
MflI RGATCY 2 cut(s) 349, 610
MhlI GDGCHC 5 cut(s) 29, 87, 166, 291, 368
MluCI AATT 4 cut(s) 559, 727, 747, 753
MmeI TCCRAC 3 cut(s) 112, 289, 432
MseI TTAA 2 cut(s) 180, 251
MspI CCGG 3 cut(s) 120, 708, 833
MspR9I CCNGG 3 cut(s) 121, 801, 833
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 801
MwoI GCNNNNNNNGC 1 cut(s) 161
NciI CCSGG 2 cut(s) 121, 833
NdeII GATC 4 cut(s) 349, 430, 586, 610
NlaIII CATG 1 cut(s) 232
NlaIV GGNNCC 2 cut(s) 612, 634
NmuCI GTSAC 1 cut(s) 3
PasI CCCWGGG 1 cut(s) 800
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 4 cut(s) 32, 199, 373, 534
PkrI GCNGC 1 cut(s) 142
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 799
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 2 cut(s) 612, 634
PspPI GGNCC 1 cut(s) 835
PsuI RGATCY 2 cut(s) 349, 610
RsaI GTAC 4 cut(s) 170, 401, 407, 459
RsaNI GTAC 4 cut(s) 169, 400, 406, 458
SacI GAGCTC 1 cut(s) 291
SalI GTCGAC 1 cut(s) 767
SaqAI TTAA 2 cut(s) 180, 251
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 4 cut(s) 349, 430, 586, 610
Sau96I GGNCC 1 cut(s) 835
ScrFI CCNGG 3 cut(s) 121, 801, 833
SduI GDGCHC 5 cut(s) 29, 87, 166, 291, 368
SetI ASST 9 cut(s) 189, 291, 315, 468, 569, 583, 601, 619, 657
SfcI CTRYAG 2 cut(s) 513, 570
SinI GGWCC 1 cut(s) 835
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 4 cut(s) 559, 727, 747, 753
SsiI CCGC 2 cut(s) 257, 521
SspMI CTAG 1 cut(s) 827
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 119, 799, 831
TaaI ACNGT 4 cut(s) 168, 450, 499, 574
TaiI ACGT 1 cut(s) 601
TaqI TCGA 6 cut(s) 107, 368, 423, 537, 664, 768
TasI AATT 4 cut(s) 559, 727, 747, 753
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 4 cut(s) 32, 199, 373, 534
Tru1I TTAA 2 cut(s) 180, 251
Tru9I TTAA 2 cut(s) 180, 251
TscAI CASTG 5 cut(s) 34, 166, 171, 691, 709
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 3 cut(s) 245, 759, 777
TspGWI ACGGA 1 cut(s) 663
TspRI CASTG 5 cut(s) 34, 166, 171, 691, 709
VneI GTGCAC 3 cut(s) 25, 83, 162
VpaK11BI GGWCC 1 cut(s) 835
XapI RAATTY 1 cut(s) 727
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.