pycom15g24100

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000780
Physical Location & Seq
Forward (+)
18292718 .. 18293962
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24100.3

Sequence Viewer

Length: 714 bp
ATGGCCTACTTCTCAACCCCGCATGTAAACTGGCGTGAACAAATTGTTGAACTACATGGGTCGTTTTTAAGACCGCTAGATACTGCTCTGGAGGTGCAGCAGGAAGCAAGTGAAATCATGGAGGCCGTGGTTTCAATAAGCAATACAGAGACGGAACTCAAACCAGTTGAAGAGAAGAGTGTGGTACAAGATAATCAATTGACAATGTATGGTGGTGGTGGTGTAATTACGACAGAGACCTCGTTAATCGCAGGGCATACTCTTGGAGCAGCATTCAAAGTGTTGTTTGATGTCCTTATGAATGTGAAGGAAAGGACCACGCTCTTTAAACCTCTGCTTGAAGACATAAAATCCACGCTCGACTCTTTAGAACCACTGATTGAAGAGATAGAAAAGTACAAGAAGGTGTTGGATCGTCCAAAAGAGGAACTACAACATTTTGAAAATCAAATGAAGAATGGGGCGGCGCTTGTTCAGAAATGCACCAAGCTTAGTCCTTGGAAAGGCTACAAAAAGTGTAGACACTCCAAGGAACTTCTGGATTTGGACAAATCTCTACAAAGACTGTTAGCTATATTTAGAGCGCCAGAAATAAGAAATACGGAGACAGTAAGGAATATATCAATGGAGGTCAGCCAAATTGAATATGAGATTGATGCTTGGCATGCAGTACCTCACGTTCCACCGTTTTATTTTGGTTGGATTTGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

238

Amino Acids

27.14

Weight (kDa)

5.46

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 520
AciI CCGC 3 cut(s) 20, 74, 464
AclWI GGATC 1 cut(s) 420
AfaI GTAC 3 cut(s) 186, 398, 672
AfiI CCNNNNNNNGG 1 cut(s) 503
AgsI TTSAA 8 cut(s) 50, 135, 170, 277, 341, 383, 443, 644
AluBI AGCT 2 cut(s) 490, 572
AluI AGCT 2 cut(s) 490, 572
Alw26I GTCTC 3 cut(s) 143, 230, 599
AlwI GGATC 1 cut(s) 420
AoxI GGCC 2 cut(s) 3, 123
ApeKI GCWGC 2 cut(s) 97, 269
AspLEI GCGC 2 cut(s) 469, 586
AspS9I GGNCC 1 cut(s) 315
AvaII GGWCC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 109, 281
BceAI ACGGC 1 cut(s) 110
BcoDI GTCTC 3 cut(s) 143, 230, 599
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 2 cut(s) 470, 587
BisI GCNGC 3 cut(s) 98, 270, 465
BlsI GCNGC 3 cut(s) 99, 271, 466
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 315
BmsI GCATC 1 cut(s) 646
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 1 cut(s) 110
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 3 cut(s) 126, 497, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 35, 164
BseDI CCNNGG 3 cut(s) 126, 497, 528
BseLI CCNNNNNNNGG 1 cut(s) 503
BseNI ACTGG 2 cut(s) 35, 164
BseXI GCAGC 2 cut(s) 109, 281
BsgI GTGCAG 1 cut(s) 116
BshFI GGCC 2 cut(s) 5, 125
BslI CCNNNNNNNGG 1 cut(s) 503
BsmAI GTCTC 3 cut(s) 143, 230, 599
BsmBI CGTCTC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 2 cut(s) 5, 125
Bso31I GGTCTC 1 cut(s) 230
Bsp143I GATC 1 cut(s) 412
BspACI CCGC 3 cut(s) 20, 74, 464
BspANI GGCC 2 cut(s) 5, 125
BspPI GGATC 1 cut(s) 420
BspTNI GGTCTC 1 cut(s) 230
BsrI ACTGG 2 cut(s) 35, 164
BssECI CCNNGG 3 cut(s) 126, 497, 528
BssMI GATC 1 cut(s) 412
BssT1I CCWWGG 2 cut(s) 497, 528
Bst4CI ACNGT 3 cut(s) 567, 610, 687
Bst6I CTCTTC 3 cut(s) 165, 170, 378
BstC8I GCNNGC 1 cut(s) 666
BstDEI CTNAG 1 cut(s) 491
BstDSI CCRYGG 1 cut(s) 126
BstENI CCTNNNNNAGG 1 cut(s) 501
BstH2I RGCGCY 2 cut(s) 470, 587
BstHHI GCGC 2 cut(s) 469, 586
BstKTI GATC 1 cut(s) 415
BstMAI GTCTC 3 cut(s) 143, 230, 599
BstMBI GATC 1 cut(s) 412
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNSI RCATGY 2 cut(s) 26, 668
BstV1I GCAGC 2 cut(s) 109, 281
BstV2I GAAGAC 1 cut(s) 348
BsuRI GGCC 2 cut(s) 5, 125
BtgI CCRYGG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 374
Cac8I GCNNGC 1 cut(s) 666
CfoI GCGC 2 cut(s) 469, 586
Cfr13I GGNCC 1 cut(s) 315
Csp6I GTAC 3 cut(s) 185, 397, 671
CviAII CATG 4 cut(s) 23, 56, 118, 665
CviJI RGCY 6 cut(s) 5, 125, 490, 507, 572, 636
CviKI_1 RGCY 6 cut(s) 5, 125, 490, 507, 572, 636
CviQI GTAC 3 cut(s) 185, 397, 671
DdeI CTNAG 1 cut(s) 491
DpnI GATC 1 cut(s) 414
DpnII GATC 1 cut(s) 412
DraI TTTAAA 1 cut(s) 328
Eam1104I CTCTTC 3 cut(s) 165, 170, 378
EarI CTCTTC 3 cut(s) 165, 170, 378
Eco130I CCWWGG 2 cut(s) 497, 528
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 315
EcoNI CCTNNNNNAGG 1 cut(s) 501
EcoT14I CCWWGG 2 cut(s) 497, 528
ErhI CCWWGG 2 cut(s) 497, 528
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 4 cut(s) 26, 59, 121, 668
FatI CATG 4 cut(s) 22, 55, 117, 664
FauI CCCGC 1 cut(s) 27
FblI GTMKAC 1 cut(s) 520
Fnu4HI GCNGC 3 cut(s) 98, 270, 465
Fsp4HI GCNGC 3 cut(s) 98, 270, 465
FspBI CTAG 1 cut(s) 77
GlaI GCGC 2 cut(s) 468, 585
GluI GCNGC 3 cut(s) 98, 270, 465
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 2 cut(s) 470, 587
HaeIII GGCC 2 cut(s) 5, 125
HhaI GCGC 2 cut(s) 469, 586
Hin1II CATG 4 cut(s) 26, 59, 121, 668
Hin6I GCGC 2 cut(s) 467, 584
HinP1I GCGC 2 cut(s) 467, 584
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 1 cut(s) 362
Hpy166II GTNNAC 3 cut(s) 28, 38, 521
Hpy188I TCNGA 1 cut(s) 477
Hpy188III TCNNGA 2 cut(s) 89, 539
Hpy8I GTNNAC 3 cut(s) 28, 38, 521
HpyAV CCTTC 2 cut(s) 301, 397
HpyCH4III ACNGT 3 cut(s) 567, 610, 687
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 3 cut(s) 97, 483, 668
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 1 cut(s) 491
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 4 cut(s) 26, 59, 121, 668
HspAI GCGC 2 cut(s) 467, 584
Kzo9I GATC 1 cut(s) 412
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 7 cut(s) 16, 74, 86, 177, 237, 524, 600
Lsp1109I GCAGC 2 cut(s) 109, 281
LweI GCATC 1 cut(s) 646
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 678
MalI GATC 1 cut(s) 414
MboI GATC 1 cut(s) 412
MboII GAAGA 5 cut(s) 182, 187, 353, 395, 466
MfeI CAATTG 1 cut(s) 197
MluCI AATT 4 cut(s) 42, 197, 225, 639
MlyI GAGTC 1 cut(s) 356
MmeI TCCRAC 2 cut(s) 390, 680
MnlI CCTC 7 cut(s) 85, 115, 250, 342, 418, 622, 684
MseI TTAA 3 cut(s) 68, 245, 327
MunI CAATTG 1 cut(s) 197
Mva1269I GAATGC 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 665
NdeII GATC 1 cut(s) 412
NlaIII CATG 4 cut(s) 26, 59, 121, 668
NspI RCATGY 2 cut(s) 26, 668
PaeI GCATGC 1 cut(s) 668
PctI GAATGC 1 cut(s) 272
PkrI GCNGC 3 cut(s) 99, 271, 466
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
PspPI GGNCC 1 cut(s) 315
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
RsaI GTAC 3 cut(s) 186, 398, 672
RsaNI GTAC 3 cut(s) 185, 397, 671
SaqAI TTAA 3 cut(s) 68, 245, 327
SatI GCNGC 3 cut(s) 98, 270, 465
Sau3AI GATC 1 cut(s) 412
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 1 cut(s) 356
SetI ASST 9 cut(s) 96, 242, 334, 408, 492, 574, 633, 676, 681
SfaNI GCATC 1 cut(s) 646
SinI GGWCC 1 cut(s) 315
SphI GCATGC 1 cut(s) 668
Sse9I AATT 4 cut(s) 42, 197, 225, 639
SsiI CCGC 3 cut(s) 20, 74, 464
SspMI CTAG 1 cut(s) 77
StyI CCWWGG 2 cut(s) 497, 528
TaaI ACNGT 3 cut(s) 567, 610, 687
TaiI ACGT 1 cut(s) 681
TaqI TCGA 1 cut(s) 360
TasI AATT 4 cut(s) 42, 197, 225, 639
TatI WGTACW 1 cut(s) 396
TauI GCSGC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 68, 245, 327
Tru9I TTAA 3 cut(s) 68, 245, 327
TscAI CASTG 1 cut(s) 381
TseI GCWGC 2 cut(s) 97, 269
TspDTI ATGAA 2 cut(s) 314, 467
TspGWI ACGGA 2 cut(s) 167, 617
TspRI CASTG 1 cut(s) 381
VpaK11BI GGWCC 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 501
XceI RCATGY 2 cut(s) 26, 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 520
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.