Rh2AG169200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000780
Physical Location & Seq
Forward (+)
16026249 .. 16027262
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG169200.1

Sequence Viewer

Length: 903 bp
ATGGACAACCTTGTTGCAGGGGGTGCTATGGGTGTACCATTTAATGCGCTCTATGATGTCATTAAGCAAGTGGTGGTCAAGACTAAGATGTTTAAGCCTCTCCTCAAAGATCTTGACTCAACGATAAATTCTTTGAAACCACTGATCGATGAGATGAAAAAGTGCAATAAGGTGTTGGATCATCCAGAGAATGAACTTAAAGGATTTGAAGTACAAATGCAGAAGGGTGCGGTGCTCATTCCCAAGTGCTCTAAGGTTAGATGGTGGAATATTTGCAAAAAGTATAAATACACCAACCAACTTGTTGGATTAAACAGCTCTCTTCGGAGTTTGTATGATATATTAGAAGTGCAAGTAGCTAGAGATATGAAGAATACGCTGGTTGAAGTAAGAAATATAGGGACGGCTGTCCAGCATACTGATACAACAATAACGGAAGTTGCAGCGAATGTGCAACAGAACTTGGCTGCGACAAATGATATTGAGATGGTGGTCCATCAGACTGATGTAACAACCTTGCAAGTGGCAAGGCATGTGAGAAATATAGATGTTGTAGTTCAGCGTACTGATGAAACAAGCTTGCAAGTAGCAAGGGATGTGAGAAACATAGCGACAGTGGTACAGCGGATTGATGACAATGTTGTGTTACTGGTACACAATCAGAAAATAGACCAGGTTCGAGGTTGGGGTGCAGTACCTGTAGGTGCACCATTTACAGTTGGAGTGGATATGCCTTTGAGGGAATTGAAGATTAAGCTTCTTAAGGATGAGAAAGTAACAATGCTTGTGCTCACTGCTCCAGGAGGATGTGGGAAAACCACTTTAGCAATAAAGTTTTGTGAAGATTACGAAGTCAAAGGACCGGCAAACGTTCACATCTCTCACTTCTCTCATCTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

300

Amino Acids

33.47

Weight (kDa)

8.69

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 137 9.8e-34 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 230, 625
AclI AACGTT 1 cut(s) 870
AclWI GGATC 1 cut(s) 186
AcsI RAATTY 1 cut(s) 127
AfaI GTAC 6 cut(s) 36, 213, 565, 621, 654, 696
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 4 cut(s) 136, 209, 386, 748
AjnI CCWGG 2 cut(s) 672, 799
AluBI AGCT 4 cut(s) 318, 359, 579, 757
AluI AGCT 4 cut(s) 318, 359, 579, 757
Alw21I GWGCWC 4 cut(s) 237, 251, 709, 792
Alw44I GTGCAC 1 cut(s) 705
AlwI GGATC 1 cut(s) 186
AlwNI CAGNNNCTG 1 cut(s) 698
ApaLI GTGCAC 1 cut(s) 705
ApeKI GCWGC 2 cut(s) 443, 467
ApoI RAATTY 1 cut(s) 127
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 493, 860
AvaII GGWCC 2 cut(s) 493, 860
BaeGI GKGCMC 1 cut(s) 709
Bbv12I GWGCWC 4 cut(s) 237, 251, 709, 792
BbvI GCAGC 2 cut(s) 454, 455
BccI CCATC 3 cut(s) 255, 481, 504
BceAI ACGGC 1 cut(s) 420
BciT130I CCWGG 2 cut(s) 674, 801
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 699
BfrI CTTAAG 1 cut(s) 761
BglII AGATCT 1 cut(s) 109
BisI GCNGC 2 cut(s) 444, 468
BlsI GCNGC 2 cut(s) 445, 469
Bme1390I CCNGG 2 cut(s) 674, 801
Bme18I GGWCC 2 cut(s) 493, 860
BmgT120I GGNCC 2 cut(s) 493, 860
BmrFI CCNGG 2 cut(s) 674, 801
BoxI GACNNNNGTC 1 cut(s) 407
BpmI CTGGAG 1 cut(s) 783
Bsa29I ATCGAT 1 cut(s) 147
Bse118I RCCGGY 1 cut(s) 862
Bse1I ACTGG 1 cut(s) 654
BseBI CCWGG 2 cut(s) 674, 801
BseCI ATCGAT 1 cut(s) 147
BseGI GGATG 4 cut(s) 181, 601, 772, 812
BseNI ACTGG 1 cut(s) 654
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 1 cut(s) 709
BseXI GCAGC 2 cut(s) 454, 455
BsgI GTGCAG 1 cut(s) 711
BshVI ATCGAT 1 cut(s) 147
BsiHKAI GWGCWC 4 cut(s) 237, 251, 709, 792
BsiSI CCGG 1 cut(s) 863
BslFI GGGAC 1 cut(s) 415
BsmFI GGGAC 1 cut(s) 415
Bsp1286I GDGCHC 4 cut(s) 237, 251, 709, 792
Bsp143I GATC 3 cut(s) 109, 144, 178
BspACI CCGC 2 cut(s) 230, 625
BspDI ATCGAT 1 cut(s) 147
BspPI GGATC 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 761
BsrFI RCCGGY 1 cut(s) 862
BsrI ACTGG 1 cut(s) 654
BssAI RCCGGY 1 cut(s) 862
BssMI GATC 3 cut(s) 109, 144, 178
Bst2UI CCWGG 2 cut(s) 674, 801
Bst4CI ACNGT 2 cut(s) 616, 718
Bst6I CTCTTC 1 cut(s) 327
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 2 cut(s) 84, 252
BstF5I GGATG 4 cut(s) 181, 601, 772, 812
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 3 cut(s) 112, 147, 181
BstMBI GATC 3 cut(s) 109, 144, 178
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 674, 801
BstNSI RCATGY 1 cut(s) 536
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 2 cut(s) 672, 799
BstSFI CTRYAG 1 cut(s) 699
BstSLI GKGCMC 1 cut(s) 709
BstV1I GCAGC 2 cut(s) 454, 455
BstX2I RGATCY 1 cut(s) 109
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 147
BsuTUI ATCGAT 1 cut(s) 147
BtsCI GGATG 4 cut(s) 181, 601, 772, 812
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 3 cut(s) 140, 621, 792
Cac8I GCNNGC 1 cut(s) 581
CaiI CAGNNNCTG 1 cut(s) 698
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 862
Cfr13I GGNCC 2 cut(s) 493, 860
ClaI ATCGAT 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 672
Csp6I GTAC 6 cut(s) 35, 212, 564, 620, 653, 695
CviAII CATG 1 cut(s) 533
CviJI RGCY 7 cut(s) 97, 318, 359, 407, 467, 579, 757
CviKI_1 RGCY 7 cut(s) 97, 318, 359, 407, 467, 579, 757
CviQI GTAC 6 cut(s) 35, 212, 564, 620, 653, 695
DdeI CTNAG 2 cut(s) 84, 252
DpnI GATC 3 cut(s) 111, 146, 180
DpnII GATC 3 cut(s) 109, 144, 178
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco47I GGWCC 2 cut(s) 493, 860
EcoRII CCWGG 2 cut(s) 672, 799
FaeI CATG 1 cut(s) 536
FaqI GGGAC 1 cut(s) 415
FatI CATG 1 cut(s) 532
Fnu4HI GCNGC 2 cut(s) 444, 468
FokI GGATG 4 cut(s) 168, 608, 779, 819
Fsp4HI GCNGC 2 cut(s) 444, 468
FspBI CTAG 1 cut(s) 360
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 444, 468
GsuI CTGGAG 1 cut(s) 783
HapII CCGG 1 cut(s) 863
HhaI GCGC 1 cut(s) 49
Hin1II CATG 1 cut(s) 536
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HindIII AAGCTT 2 cut(s) 577, 755
HinfI GANTC 1 cut(s) 116
HpaII CCGG 1 cut(s) 863
Hpy166II GTNNAC 4 cut(s) 35, 655, 707, 874
Hpy188I TCNGA 3 cut(s) 327, 501, 663
Hpy188III TCNNGA 3 cut(s) 79, 113, 185
Hpy8I GTNNAC 4 cut(s) 35, 655, 707, 874
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 2 cut(s) 616, 718
HpyCH4IV ACGT 1 cut(s) 870
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 2 cut(s) 84, 252
HpySE526I ACGT 1 cut(s) 870
Hsp92II CATG 1 cut(s) 536
HspAI GCGC 1 cut(s) 47
Kzo9I GATC 3 cut(s) 109, 144, 178
LmnI GCTCC 1 cut(s) 802
Lsp1109I GCAGC 2 cut(s) 454, 455
MabI ACCWGGT 1 cut(s) 672
MaeI CTAG 1 cut(s) 360
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 3 cut(s) 508, 645, 775
MalI GATC 3 cut(s) 111, 146, 180
MboI GATC 3 cut(s) 109, 144, 178
MboII GAAGA 4 cut(s) 314, 382, 760, 854
MflI RGATCY 1 cut(s) 109
MhlI GDGCHC 4 cut(s) 237, 251, 709, 792
MluCI AATT 2 cut(s) 127, 743
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 3 cut(s) 156, 286, 700
MnlI CCTC 5 cut(s) 108, 113, 674, 732, 797
MseI TTAA 7 cut(s) 42, 63, 93, 198, 311, 753, 762
MspA1I CMGCKG 1 cut(s) 625
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 1 cut(s) 863
MspR9I CCNGG 2 cut(s) 674, 801
MvaI CCWGG 2 cut(s) 674, 801
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 3 cut(s) 109, 144, 178
NlaIII CATG 1 cut(s) 536
NspI RCATGY 1 cut(s) 536
PfoI TCCNGGA 1 cut(s) 799
PkrI GCNGC 2 cut(s) 445, 469
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PshAI GACNNNNGTC 1 cut(s) 407
Psp1406I AACGTT 1 cut(s) 870
Psp6I CCWGG 2 cut(s) 672, 799
PspGI CCWGG 2 cut(s) 672, 799
PspPI GGNCC 2 cut(s) 493, 860
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 1 cut(s) 109
RsaI GTAC 6 cut(s) 36, 213, 565, 621, 654, 696
RsaNI GTAC 6 cut(s) 35, 212, 564, 620, 653, 695
SaqAI TTAA 7 cut(s) 42, 63, 93, 198, 311, 753, 762
SatI GCNGC 2 cut(s) 444, 468
Sau3AI GATC 3 cut(s) 109, 144, 178
Sau96I GGNCC 2 cut(s) 493, 860
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 674, 801
SduI GDGCHC 4 cut(s) 237, 251, 709, 792
SexAI ACCWGGT 1 cut(s) 672
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 2 cut(s) 493, 860
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 2 cut(s) 127, 743
SsiI CCGC 2 cut(s) 230, 625
SspI AATATT 1 cut(s) 271
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 2 cut(s) 672, 799
TaaI ACNGT 2 cut(s) 616, 718
TaiI ACGT 1 cut(s) 873
TaqI TCGA 2 cut(s) 147, 679
TasI AATT 2 cut(s) 127, 743
TatI WGTACW 1 cut(s) 211
Tru1I TTAA 7 cut(s) 42, 63, 93, 198, 311, 753, 762
Tru9I TTAA 7 cut(s) 42, 63, 93, 198, 311, 753, 762
TscAI CASTG 3 cut(s) 147, 621, 799
TseI GCWGC 2 cut(s) 443, 467
TspDTI ATGAA 4 cut(s) 170, 207, 383, 585
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 3 cut(s) 147, 621, 799
Vha464I CTTAAG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 705
VpaK11BI GGWCC 2 cut(s) 493, 860
XapI RAATTY 1 cut(s) 127
XceI RCATGY 1 cut(s) 536
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.