Rh7DG408400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000780
Physical Location & Seq
Forward (+)
57307184 .. 57307549
366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG408400.1

Sequence Viewer

Length: 366 bp
ATGGAGGAGATGATCATGTTCAAAGTCCATGTGAGAGATGTGAAATCCACGCTCGACTGTTTGAAACCAGTGATCCAGGAGATAACGGAATACAACGAGGTGTTGAAGAATCAACCAATGGAGGAGGAACAAGCACTGCAGCACTTGAAATCGCAAATAGAAGAAGGCGCAATTCTGGTTCAAAAGTGTTCCAAGGTTGGTGCCTGGAGTTTCCGCAAAAAGTACAAGTACAGCAACCAACTCTTTCAACTCGATCAATCGCTTCGATCACACACTCTTGCAATTGCTCGAACAGCAGAAAGCAAGAGATGTCTGGGAGACCTTGGTTACGGTCAGGAAGATAGAGACCGTCGTCCAGCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

121

Amino Acids

14.09

Weight (kDa)

7.71

Isoelectric Point (pI)

54.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 4 - 90 9.7e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 1 cut(s) 214
AclWI GGATC 1 cut(s) 67
AfaI GTAC 2 cut(s) 224, 230
AgsI TTSAA 6 cut(s) 22, 64, 106, 148, 182, 248
AjnI CCWGG 2 cut(s) 75, 203
Alw26I GTCTC 2 cut(s) 312, 339
AlwI GGATC 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 139
AspLEI GCGC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 151
BciT130I CCWGG 2 cut(s) 77, 205
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 312, 339
BfmI CTRYAG 1 cut(s) 137
BisI GCNGC 1 cut(s) 140
BlsI GCNGC 1 cut(s) 141
Bme1390I CCNGG 2 cut(s) 77, 205
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 2 cut(s) 77, 205
BoxI GACNNNNGTC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 226
BsaI GGTCTC 2 cut(s) 312, 339
BsaJI CCNNGG 2 cut(s) 192, 322
Bse1I ACTGG 1 cut(s) 68
BseBI CCWGG 2 cut(s) 77, 205
BseDI CCNNGG 2 cut(s) 192, 322
BseNI ACTGG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 20, 137
BseXI GCAGC 1 cut(s) 151
BshNI GGYRCC 1 cut(s) 200
BsmAI GTCTC 2 cut(s) 312, 339
Bso31I GGTCTC 2 cut(s) 312, 339
Bsp143I GATC 4 cut(s) 12, 72, 253, 266
BspACI CCGC 1 cut(s) 214
BspLI GGNNCC 1 cut(s) 202
BspMAI CTGCAG 1 cut(s) 141
BspPI GGATC 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 2 cut(s) 312, 339
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 192, 322
BssMI GATC 4 cut(s) 12, 72, 253, 266
BssT1I CCWWGG 2 cut(s) 192, 322
Bst2UI CCWGG 2 cut(s) 77, 205
Bst4CI ACNGT 3 cut(s) 59, 332, 350
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 4 cut(s) 15, 75, 256, 269
BstMAI GTCTC 2 cut(s) 312, 339
BstMBI GATC 4 cut(s) 12, 72, 253, 266
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 2 cut(s) 77, 205
BstPAI GACNNNNGTC 1 cut(s) 351
BstSCI CCNGG 2 cut(s) 75, 203
BstSFI CTRYAG 1 cut(s) 137
BstV1I GCAGC 1 cut(s) 151
BtsI GCAGTG 1 cut(s) 134
BtsIMutI CAGTG 2 cut(s) 75, 134
CfoI GCGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 223, 229
CviAII CATG 2 cut(s) 16, 29
CviQI GTAC 2 cut(s) 223, 229
DpnI GATC 4 cut(s) 14, 74, 255, 268
DpnII GATC 4 cut(s) 12, 72, 253, 266
Eco130I CCWWGG 2 cut(s) 192, 322
Eco31I GGTCTC 2 cut(s) 312, 339
EcoRII CCWGG 2 cut(s) 75, 203
EcoT14I CCWWGG 2 cut(s) 192, 322
ErhI CCWWGG 2 cut(s) 192, 322
FaeI CATG 2 cut(s) 19, 32
FaiI YATR 2 cut(s) 17, 30
FatI CATG 2 cut(s) 15, 28
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 1 cut(s) 140
Fsp4HI GCNGC 1 cut(s) 140
GlaI GCGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 140
GsuI CTGGAG 1 cut(s) 226
HhaI GCGC 1 cut(s) 170
Hin1II CATG 2 cut(s) 19, 32
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HinfI GANTC 1 cut(s) 109
Hpy188III TCNNGA 1 cut(s) 335
Hpy99I CGWCG 1 cut(s) 354
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 3 cut(s) 59, 332, 350
HpyCH4V TGCA 2 cut(s) 139, 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
Hsp92II CATG 2 cut(s) 19, 32
HspAI GCGC 1 cut(s) 168
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 4 cut(s) 12, 72, 253, 266
LpnPI CCDG 8 cut(s) 62, 81, 89, 161, 190, 217, 299, 320
Lsp1109I GCAGC 1 cut(s) 151
MaeIII GTNAC 1 cut(s) 326
MalI GATC 4 cut(s) 14, 74, 255, 268
MboI GATC 4 cut(s) 12, 72, 253, 266
MboII GAAGA 3 cut(s) 118, 173, 350
MfeI CAATTG 1 cut(s) 282
MluCI AATT 3 cut(s) 171, 282, 361
MnlI CCTC 3 cut(s) 91, 115, 118
MspR9I CCNGG 2 cut(s) 77, 205
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 2 cut(s) 77, 205
MwoI GCNNNNNNNGC 1 cut(s) 293
NdeII GATC 4 cut(s) 12, 72, 253, 266
NlaIII CATG 2 cut(s) 19, 32
NlaIV GGNNCC 1 cut(s) 202
PfeI GAWTC 1 cut(s) 109
PfoI TCCNGGA 1 cut(s) 75
PkrI GCNGC 1 cut(s) 141
PshAI GACNNNNGTC 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 75, 203
PspGI CCWGG 2 cut(s) 75, 203
PspN4I GGNNCC 1 cut(s) 202
PstI CTGCAG 1 cut(s) 141
RsaI GTAC 2 cut(s) 224, 230
RsaNI GTAC 2 cut(s) 223, 229
SatI GCNGC 1 cut(s) 140
Sau3AI GATC 4 cut(s) 12, 72, 253, 266
ScrFI CCNGG 2 cut(s) 77, 205
SetI ASST 3 cut(s) 102, 198, 324
SfcI CTRYAG 1 cut(s) 137
Sse9I AATT 3 cut(s) 171, 282, 361
SsiI CCGC 1 cut(s) 214
StyD4I CCNGG 2 cut(s) 75, 203
StyI CCWWGG 2 cut(s) 192, 322
TaaI ACNGT 3 cut(s) 59, 332, 350
TaqI TCGA 4 cut(s) 54, 252, 265, 289
TasI AATT 3 cut(s) 171, 282, 361
TatI WGTACW 2 cut(s) 222, 228
TfiI GAWTC 1 cut(s) 109
TscAI CASTG 2 cut(s) 75, 141
TseI GCWGC 1 cut(s) 139
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 75, 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.