Rh7AG413700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000780
Physical Location & Seq
Forward (+)
55528156 .. 55544285
16130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG413700.1

Sequence Viewer

Length: 831 bp
ATGATTGACGGCGTTATAGAACTGCTCCGTCAGATGATCATGTTCAAGGTCCACGTGAGAGATGTGAAACTGACGCTGGAGTGTTTGAAACCAGTAATCCAAGAGATATCAGAGTATAACAAGTTGTTGAATAATCTACCAATGGAGGAGGAGCTAGCACTGCAGGACTTGAAATTGCAAATGGAGGAGGGCGCTAATCTGGTCCGAAAGTGTTCCAAGGTTGGTGCCTGGAGTTTCTGCAAAAAGTATAAGTACAGCAACCAACTCTTTCAACTGGATCAATCGCTTCAAACACTGTTGCATTTGCTCGAAGTTCAGAAAACAAGGGATGTCCAGGAGACCTTAGTTTCCGTAAAGAACATAGAGACTGTGGTCCAGCGAATTGAAGGCAATATTTCTGCCATGCAGAGTAATCAATCCGCAACGTACAACACAGATAAGAGCGCTGCCGCCACAAACTTCTTCGTTTTCTCCAATGACGCCAAGCTCCGAGCTTCCCTCACCGACGACACCATCGTCGGCGGCCCCAGCCTCAACGGCTTGGTTCTAGTCGTCAAAAATCCCGGTGTGTTCATTGTCGACTACAACGTCCCCAAAAAGAGTTCCAAAATTGATCGTCGTTCTCACAAGAAGACCGGTAACAATCTCCAACGCATCGTTGAGTCCTCGAACGGAAATCTCTATATCTTCGCTCTCGCTTGCTCCTCCTCCAACCACGACCACGACGATAGAGACAACGACAGCTCTAGAAAACGCATGAGGCAAGACCACCGTGGCAACACCGTGGTGCCCATCAGGTCCATCATGAAACCTTATGACGCTGGGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

276

Amino Acids

31.23

Weight (kDa)

8.91

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 125 1.5e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 515, 587
AccB1I GGYRCC 2 cut(s) 224, 787
AccI GTMKAC 1 cut(s) 579
AciI CCGC 3 cut(s) 420, 450, 522
AclWI GGATC 1 cut(s) 285
AcvI CACGTG 1 cut(s) 55
AcyI GRCGYC 1 cut(s) 480
AfaI GTAC 2 cut(s) 254, 428
AfeI AGCGCT 1 cut(s) 445
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 7 cut(s) 46, 88, 130, 172, 272, 290, 386
AjnI CCWGG 2 cut(s) 227, 333
AleI CACNNNNGTG 1 cut(s) 785
AluBI AGCT 4 cut(s) 154, 487, 494, 744
AluI AGCT 4 cut(s) 154, 487, 494, 744
Alw26I GTCTC 3 cut(s) 332, 359, 726
AlwI GGATC 1 cut(s) 285
Aor51HI AGCGCT 1 cut(s) 445
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 1 cut(s) 446
AsiGI ACCGGT 1 cut(s) 635
Asp700I GAANNNNTTC 1 cut(s) 211
AspLEI GCGC 2 cut(s) 194, 446
AspS9I GGNCC 5 cut(s) 49, 202, 373, 524, 798
AsuC2I CCSGG 1 cut(s) 564
AsuHPI GGTGA 1 cut(s) 493
AsuNHI GCTAGC 1 cut(s) 154
AvaII GGWCC 4 cut(s) 49, 202, 373, 798
BaeGI GKGCMC 1 cut(s) 792
BanI GGYRCC 2 cut(s) 224, 787
BbrPI CACGTG 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 638
BbvI GCAGC 1 cut(s) 433
BccI CCATC 3 cut(s) 521, 800, 809
BceAI ACGGC 2 cut(s) 25, 553
BciT130I CCWGG 2 cut(s) 229, 335
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 1 cut(s) 564
BcoDI GTCTC 3 cut(s) 332, 359, 726
BfaI CTAG 3 cut(s) 155, 548, 747
BfmI CTRYAG 1 cut(s) 161
BfoI RGCGCY 2 cut(s) 195, 447
BglI GCCNNNNNGGC 1 cut(s) 537
BisI GCNGC 3 cut(s) 447, 450, 523
BlsI GCNGC 3 cut(s) 448, 451, 524
Bme1390I CCNGG 3 cut(s) 229, 335, 564
Bme18I GGWCC 4 cut(s) 49, 202, 373, 798
BmgT120I GGNCC 5 cut(s) 49, 202, 373, 524, 798
BmiI GGNNCC 3 cut(s) 226, 526, 789
BmrFI CCNGG 3 cut(s) 229, 335, 564
BmsI GCATC 1 cut(s) 663
BmtI GCTAGC 1 cut(s) 158
BoxI GACNNNNGTC 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 638
BplI GAGNNNNNCTC 2 cut(s) 483, 515
BpmI CTGGAG 2 cut(s) 98, 250
BpuMI CCSGG 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 3 cut(s) 216, 772, 783
BsaWI WCCGGW 1 cut(s) 635
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 2 cut(s) 92, 279
BseBI CCWGG 2 cut(s) 229, 335
BseDI CCNNGG 3 cut(s) 216, 772, 783
BseGI GGATG 1 cut(s) 334
BseNI ACTGG 2 cut(s) 92, 279
BseRI GAGGAG 5 cut(s) 161, 164, 200, 694, 697
BseSI GKGCMC 1 cut(s) 792
BseXI GCAGC 1 cut(s) 433
BseYI CCCAGC 2 cut(s) 527, 821
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 2 cut(s) 224, 787
BshTI ACCGGT 1 cut(s) 635
BsiSI CCGG 2 cut(s) 564, 636
BslFI GGGAC 1 cut(s) 575
BsmAI GTCTC 3 cut(s) 332, 359, 726
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 1 cut(s) 525
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 792
Bsp143I GATC 4 cut(s) 36, 277, 613, 825
BspACI CCGC 3 cut(s) 420, 450, 522
BspANI GGCC 1 cut(s) 525
BspHI TCATGA 1 cut(s) 804
BspLI GGNNCC 3 cut(s) 226, 526, 789
BspMAI CTGCAG 1 cut(s) 165
BspOI GCTAGC 1 cut(s) 158
BspPI GGATC 1 cut(s) 285
BspT107I GGYRCC 2 cut(s) 224, 787
BspTNI GGTCTC 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 2 cut(s) 92, 279
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 216, 772, 783
BssMI GATC 4 cut(s) 36, 277, 613, 825
BssNI GRCGYC 1 cut(s) 480
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 2 cut(s) 229, 335
Bst4CI ACNGT 4 cut(s) 297, 370, 773, 784
BstACI GRCGYC 1 cut(s) 480
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 156, 700
BstDEI CTNAG 1 cut(s) 343
BstDSI CCRYGG 2 cut(s) 772, 783
BstF5I GGATG 1 cut(s) 334
BstH2I RGCGCY 2 cut(s) 195, 447
BstHHI GCGC 2 cut(s) 194, 446
BstKTI GATC 4 cut(s) 39, 280, 616, 828
BstMAI GTCTC 3 cut(s) 332, 359, 726
BstMBI GATC 4 cut(s) 36, 277, 613, 825
BstMWI GCNNNNNNNGC 3 cut(s) 160, 528, 537
BstNI CCWGG 2 cut(s) 229, 335
BstPAI GACNNNNGTC 1 cut(s) 371
BstSCI CCNGG 3 cut(s) 227, 333, 562
BstSFI CTRYAG 1 cut(s) 161
BstSLI GKGCMC 1 cut(s) 792
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 1 cut(s) 825
BstYI RGATCY 1 cut(s) 825
BsuRI GGCC 1 cut(s) 525
BtgI CCRYGG 2 cut(s) 772, 783
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 158
BtsIMutI CAGTG 2 cut(s) 158, 293
Cac8I GCNNGC 2 cut(s) 156, 700
CciI TCATGA 1 cut(s) 804
CfoI GCGC 2 cut(s) 194, 446
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 5 cut(s) 49, 202, 373, 524, 798
CseI GACGC 2 cut(s) 82, 488
Csp6I GTAC 2 cut(s) 253, 427
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 4 cut(s) 40, 403, 757, 805
CviJI RGCY 7 cut(s) 154, 487, 494, 525, 531, 540, 744
CviKI_1 RGCY 7 cut(s) 154, 487, 494, 525, 531, 540, 744
CviQI GTAC 2 cut(s) 253, 427
DdeI CTNAG 1 cut(s) 343
DpnI GATC 4 cut(s) 38, 279, 615, 827
DpnII GATC 4 cut(s) 36, 277, 613, 825
DrdI GACNNNNNNGTC 2 cut(s) 515, 587
DseDI GACNNNNNNGTC 2 cut(s) 515, 587
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 332
Eco32I GATATC 1 cut(s) 108
Eco47I GGWCC 4 cut(s) 49, 202, 373, 798
Eco47III AGCGCT 1 cut(s) 445
Eco72I CACGTG 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 227, 333
EcoRV GATATC 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 4 cut(s) 43, 406, 760, 808
FaqI GGGAC 1 cut(s) 575
FatI CATG 4 cut(s) 39, 402, 756, 804
FbaI TGATCA 1 cut(s) 36
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 3 cut(s) 447, 450, 523
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 3 cut(s) 447, 450, 523
FspBI CTAG 3 cut(s) 155, 548, 747
GlaI GCGC 2 cut(s) 193, 445
GluI GCNGC 3 cut(s) 447, 450, 523
GsaI CCCAGC 2 cut(s) 531, 825
GsuI CTGGAG 2 cut(s) 98, 250
HaeII RGCGCY 2 cut(s) 195, 447
HaeIII GGCC 1 cut(s) 525
HapII CCGG 2 cut(s) 564, 636
HgaI GACGC 2 cut(s) 82, 488
HhaI GCGC 2 cut(s) 194, 446
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 4 cut(s) 43, 406, 760, 808
Hin6I GCGC 2 cut(s) 192, 444
HinP1I GCGC 2 cut(s) 192, 444
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 1 cut(s) 662
HpaII CCGG 2 cut(s) 564, 636
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 2 cut(s) 52, 580
Hpy188I TCNGA 6 cut(s) 33, 112, 206, 318, 491, 830
Hpy188III TCNNGA 2 cut(s) 747, 805
Hpy8I GTNNAC 2 cut(s) 52, 580
Hpy99I CGWCG 4 cut(s) 509, 521, 621, 728
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 4 cut(s) 297, 370, 773, 784
HpyCH4IV ACGT 3 cut(s) 54, 425, 588
HpyCH4V TGCA 5 cut(s) 163, 178, 240, 301, 406
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 528, 537
HpyF3I CTNAG 1 cut(s) 343
HpySE526I ACGT 3 cut(s) 54, 425, 588
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 4 cut(s) 43, 406, 760, 808
HspAI GCGC 2 cut(s) 192, 444
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 4 cut(s) 36, 277, 613, 825
LmnI GCTCC 4 cut(s) 30, 151, 492, 707
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 1 cut(s) 663
MaeI CTAG 3 cut(s) 155, 548, 747
MaeII ACGT 3 cut(s) 54, 425, 588
MaeIII GTNAC 1 cut(s) 638
MalI GATC 4 cut(s) 38, 279, 615, 827
MboI GATC 4 cut(s) 36, 277, 613, 825
MboII GAAGA 3 cut(s) 454, 643, 679
MflI RGATCY 1 cut(s) 825
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 3 cut(s) 173, 381, 609
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 2 cut(s) 673, 735
MroXI GAANNNNTTC 1 cut(s) 211
MslI CAYNNNNRTG 1 cut(s) 785
MspI CCGG 2 cut(s) 564, 636
MspR9I CCNGG 3 cut(s) 229, 335, 564
MvaI CCWGG 2 cut(s) 229, 335
MwoI GCNNNNNNNGC 3 cut(s) 160, 528, 537
NciI CCSGG 1 cut(s) 564
NdeII GATC 4 cut(s) 36, 277, 613, 825
NheI GCTAGC 1 cut(s) 154
NlaIII CATG 4 cut(s) 43, 406, 760, 808
NlaIV GGNNCC 3 cut(s) 226, 526, 789
OliI CACNNNNGTG 1 cut(s) 785
PagI TCATGA 1 cut(s) 804
PcsI WCGNNNNNNNCGW 3 cut(s) 513, 585, 723
PdmI GAANNNNTTC 1 cut(s) 211
PfoI TCCNGGA 1 cut(s) 333
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 3 cut(s) 448, 451, 524
PleI GAGTC 1 cut(s) 670
PmaCI CACGTG 1 cut(s) 55
PmlI CACGTG 1 cut(s) 55
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 55
PshAI GACNNNNGTC 1 cut(s) 371
Psp6I CCWGG 2 cut(s) 227, 333
PspCI CACGTG 1 cut(s) 55
PspFI CCCAGC 2 cut(s) 527, 821
PspGI CCWGG 2 cut(s) 227, 333
PspN4I GGNNCC 3 cut(s) 226, 526, 789
PspPI GGNCC 5 cut(s) 49, 202, 373, 524, 798
PstI CTGCAG 1 cut(s) 165
PsuI RGATCY 1 cut(s) 825
RsaI GTAC 2 cut(s) 254, 428
RsaNI GTAC 2 cut(s) 253, 427
RseI CAYNNNNRTG 1 cut(s) 785
SalI GTCGAC 1 cut(s) 578
SatI GCNGC 3 cut(s) 447, 450, 523
Sau3AI GATC 4 cut(s) 36, 277, 613, 825
Sau96I GGNCC 5 cut(s) 49, 202, 373, 524, 798
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 3 cut(s) 229, 335, 564
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 1 cut(s) 663
SfcI CTRYAG 1 cut(s) 161
SinI GGWCC 4 cut(s) 49, 202, 373, 798
SmiMI CAYNNNNRTG 1 cut(s) 785
Sse9I AATT 3 cut(s) 173, 381, 609
SsiI CCGC 3 cut(s) 420, 450, 522
SspI AATATT 1 cut(s) 394
SspMI CTAG 3 cut(s) 155, 548, 747
StyD4I CCNGG 3 cut(s) 227, 333, 562
StyI CCWWGG 1 cut(s) 216
TaaI ACNGT 4 cut(s) 297, 370, 773, 784
TaiI ACGT 3 cut(s) 57, 428, 591
TaqI TCGA 3 cut(s) 309, 579, 668
TasI AATT 3 cut(s) 173, 381, 609
TatI WGTACW 1 cut(s) 252
TauI GCSGC 2 cut(s) 452, 525
TscAI CASTG 2 cut(s) 165, 300
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 2 cut(s) 562, 821
TspGWI ACGGA 3 cut(s) 17, 340, 687
TspRI CASTG 2 cut(s) 165, 300
VpaK11BI GGWCC 4 cut(s) 49, 202, 373, 798
XbaI TCTAGA 1 cut(s) 746
XmiI GTMKAC 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 3 cut(s) 155, 548, 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.