Rh7DG409300

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000780
Physical Location & Seq
Forward (+)
57412915 .. 57495391
82477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG409300.1

Sequence Viewer

Length: 540 bp
ATGACCTTCAGTTTTTATTGGGTCTGTGTACAAGAAGATAGTAAAGAGAAAGGAAAAAAAAGCTCCAAGCTCCGGCCAGCAGCCCAAGCACGTACTGTTGTTGTTCTTAATATGATTGACGGCGTTATAGAACTGCTCCGTCAGATGATCATGTTCAAGGTCCACGTGAGAGATGTGAAACTGACGCTGGACTGTTTGAAACCAGTAGTCCAAGAGATATCAGAGTATAACAAGTTGTTGAATAATCTACCAATGGAGGAAGAGCTAGCATGGCAGGACTTGAAATTGCAAATGGAGGAGGGCGCTAATCTGGTTCGAAAGTGTTCCAAGGTTGGTGCCTGGAGTTTCTGCAAAAAGTATAGATACACCACCCAACTTCATCGACTCGATAAATCGCTGCACACGCTCTTGCATCTACTGGAACTGCAGAAAACAAGGGATATCAGGGAGACCTTGGTTTCGGTAAGGAACATAGAGACCACCGTCCAGCGAATTGAGGGCAATATTTGTGTCAGGCAAAATCAGTCTGGAACTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

179

Amino Acids

20.88

Weight (kDa)

9.22

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 39 - 163 9.8e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 74
AcvI CACGTG 1 cut(s) 166
AfaI GTAC 2 cut(s) 30, 94
AfiI CCNNNNNNNGG 1 cut(s) 72
AgsI TTSAA 4 cut(s) 157, 199, 241, 283
AjnI CCWGG 1 cut(s) 338
AluBI AGCT 3 cut(s) 63, 70, 265
AluI AGCT 3 cut(s) 63, 70, 265
Alw26I GTCTC 2 cut(s) 443, 470
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 2 cut(s) 80, 397
ArsI GACNNNNNNTTYG 2 cut(s) 442, 474
Asp700I GAANNNNTTC 1 cut(s) 322
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 160
AsuII TTCGAA 1 cut(s) 316
AsuNHI GCTAGC 1 cut(s) 265
AvaII GGWCC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 335
BbrPI CACGTG 1 cut(s) 166
BbvI GCAGC 2 cut(s) 92, 384
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 340
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 443, 470
BfaI CTAG 1 cut(s) 266
BfmI CTRYAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 306
BisI GCNGC 2 cut(s) 81, 398
BlsI GCNGC 2 cut(s) 82, 399
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 1 cut(s) 340
BmsI GCATC 1 cut(s) 421
BmtI GCTAGC 1 cut(s) 269
BoxI GACNNNNGTC 1 cut(s) 482
BpmI CTGGAG 1 cut(s) 361
Bpu14I TTCGAA 1 cut(s) 316
BsaAI YACGTR 2 cut(s) 92, 166
BsaI GGTCTC 2 cut(s) 443, 470
BsaJI CCNNGG 2 cut(s) 327, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 2 cut(s) 203, 423
BseBI CCWGG 1 cut(s) 340
BseDI CCNNGG 2 cut(s) 327, 453
BseLI CCNNNNNNNGG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 203, 423
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 2 cut(s) 92, 384
BsgI GTGCAG 1 cut(s) 383
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 335
BsiSI CCGG 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 2 cut(s) 443, 470
BsnI GGCC 1 cut(s) 76
Bso31I GGTCTC 2 cut(s) 443, 470
Bsp119I TTCGAA 1 cut(s) 316
Bsp1407I TGTACA 1 cut(s) 28
Bsp143I GATC 1 cut(s) 147
BspANI GGCC 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 337
BspMAI CTGCAG 1 cut(s) 429
BspOI GCTAGC 1 cut(s) 269
BspQI GCTCTTC 1 cut(s) 255
BspT104I TTCGAA 1 cut(s) 316
BspT107I GGYRCC 1 cut(s) 335
BspTNI GGTCTC 2 cut(s) 443, 470
BsrGI TGTACA 1 cut(s) 28
BsrI ACTGG 2 cut(s) 203, 423
BssECI CCNNGG 2 cut(s) 327, 453
BssMI GATC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 327, 453
Bst2UI CCWGG 1 cut(s) 340
Bst4CI ACNGT 3 cut(s) 97, 194, 484
Bst6I CTCTTC 1 cut(s) 255
BstAUI TGTACA 1 cut(s) 28
BstBAI YACGTR 2 cut(s) 92, 166
BstBI TTCGAA 1 cut(s) 316
BstC8I GCNNGC 2 cut(s) 78, 267
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 2 cut(s) 443, 470
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 3 cut(s) 86, 271, 403
BstNI CCWGG 1 cut(s) 340
BstPAI GACNNNNGTC 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 338
BstSFI CTRYAG 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 92, 384
BsuRI GGCC 1 cut(s) 76
Cac8I GCNNGC 2 cut(s) 78, 267
CfoI GCGC 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 160
CseI GACGC 1 cut(s) 193
Csp6I GTAC 2 cut(s) 29, 93
CviAII CATG 2 cut(s) 151, 270
CviJI RGCY 5 cut(s) 63, 70, 76, 83, 265
CviKI_1 RGCY 5 cut(s) 63, 70, 76, 83, 265
CviQI GTAC 2 cut(s) 29, 93
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
EaeI YGGCCR 1 cut(s) 74
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
Eco130I CCWWGG 2 cut(s) 327, 453
Eco31I GGTCTC 2 cut(s) 443, 470
Eco32I GATATC 2 cut(s) 219, 442
Eco47I GGWCC 1 cut(s) 160
Eco72I CACGTG 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 338
EcoRV GATATC 2 cut(s) 219, 442
EcoT14I CCWWGG 2 cut(s) 327, 453
ErhI CCWWGG 2 cut(s) 327, 453
FaeI CATG 2 cut(s) 154, 273
FaiI YATR 7 cut(s) 113, 128, 152, 228, 271, 360, 473
FatI CATG 2 cut(s) 150, 269
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 2 cut(s) 81, 398
Fsp4HI GCNGC 2 cut(s) 81, 398
FspBI CTAG 1 cut(s) 266
GlaI GCGC 1 cut(s) 304
GluI GCNGC 2 cut(s) 81, 398
GsuI CTGGAG 1 cut(s) 361
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 1 cut(s) 76
HapII CCGG 1 cut(s) 73
HgaI GACGC 1 cut(s) 193
HhaI GCGC 1 cut(s) 305
Hin1II CATG 2 cut(s) 154, 273
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HinfI GANTC 1 cut(s) 384
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 29, 163
Hpy188I TCNGA 2 cut(s) 144, 223
Hpy188III TCNNGA 1 cut(s) 528
Hpy8I GTNNAC 2 cut(s) 29, 163
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 3 cut(s) 97, 194, 484
HpyCH4IV ACGT 2 cut(s) 91, 165
HpyCH4V TGCA 5 cut(s) 289, 351, 400, 412, 427
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 271, 403
HpySE526I ACGT 2 cut(s) 91, 165
Hsp92II CATG 2 cut(s) 154, 273
HspAI GCGC 1 cut(s) 303
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 1 cut(s) 147
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 3 cut(s) 68, 75, 141
Lsp1109I GCAGC 2 cut(s) 92, 384
LweI GCATC 1 cut(s) 421
MaeI CTAG 1 cut(s) 266
MaeII ACGT 2 cut(s) 91, 165
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 2 cut(s) 47, 272
MluCI AATT 3 cut(s) 284, 492, 535
MlyI GAGTC 1 cut(s) 378
MnlI CCTC 4 cut(s) 250, 289, 292, 490
MroXI GAANNNNTTC 1 cut(s) 322
MseI TTAA 1 cut(s) 108
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 1 cut(s) 340
MvaI CCWGG 1 cut(s) 340
MwoI GCNNNNNNNGC 3 cut(s) 86, 271, 403
NdeII GATC 1 cut(s) 147
NheI GCTAGC 1 cut(s) 265
NlaIII CATG 2 cut(s) 154, 273
NlaIV GGNNCC 1 cut(s) 337
NspV TTCGAA 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 255
PdmI GAANNNNTTC 1 cut(s) 322
PkrI GCNGC 2 cut(s) 82, 399
PleI GAGTC 1 cut(s) 378
PmaCI CACGTG 1 cut(s) 166
PmlI CACGTG 1 cut(s) 166
PpsI GAGTC 1 cut(s) 378
Ppu21I YACGTR 2 cut(s) 92, 166
PshAI GACNNNNGTC 1 cut(s) 482
Psp6I CCWGG 1 cut(s) 338
PspCI CACGTG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 160
PstI CTGCAG 1 cut(s) 429
RsaI GTAC 2 cut(s) 30, 94
RsaNI GTAC 2 cut(s) 29, 93
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 2 cut(s) 81, 398
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 1 cut(s) 378
ScrFI CCNGG 1 cut(s) 340
SetI ASST 9 cut(s) 8, 65, 72, 94, 162, 168, 267, 333, 455
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 1 cut(s) 425
SfuI TTCGAA 1 cut(s) 316
SinI GGWCC 1 cut(s) 160
Sse9I AATT 3 cut(s) 284, 492, 535
SspI AATATT 1 cut(s) 505
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 1 cut(s) 338
StyI CCWWGG 2 cut(s) 327, 453
TaaI ACNGT 3 cut(s) 97, 194, 484
TaiI ACGT 2 cut(s) 94, 168
TaqI TCGA 3 cut(s) 316, 382, 387
TasI AATT 3 cut(s) 284, 492, 535
TatI WGTACW 1 cut(s) 28
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseI GCWGC 2 cut(s) 80, 397
TspDTI ATGAA 1 cut(s) 368
TspGWI ACGGA 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 160
XmnI GAANNNNTTC 1 cut(s) 322
XspI CTAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.