Rh2DG177700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000780
Physical Location & Seq
Forward (+)
15605804 .. 15606310
507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG177700.1

Sequence Viewer

Length: 507 bp
ATGACAGTGGCATCATTAGAAGGACGAGTGGAAGCAGCATTTCTTGCCCTGTTTGGTATTGTTTTGGAAGTGAAGGACAAGAATGTGATGTATAGACCACTTATGGGATCTATCAAATCCAGTCTGGACTGCTTAAAGCCATTGATCGAAGACATGGCACAAGAAAACAGGGTCCTGGATCGTCCAGTTAAGGAACTAGAGTATTTCAGAATGCATATGGAGAAAGGTGCAGAGCTAGTCCGCAAGTGCTCCAAAAATGGTGTGTGCGCCAAATACAAAAAGAAGAAATACACCAACCAACTTCTTGAGTTGGATGCATGTCTTCAGAGGCAGTTCAATGTACTGAGAGCGCAGGTTGCAAGCGATGTGAGGGAGACCACAGCTTCGATAAAGAGTATACAGAAGGTGATCAAGCGAATTGAGGAGAGTGGTGTCATGAAGAATCAAATGGAAATGAATGGTCCGTGTGAAGTAGTTGAGGCTTCATCCCCTGATGAACAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

168

Amino Acids

19.08

Weight (kDa)

8.76

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 11 - 138 1.6e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 397
AciI CCGC 1 cut(s) 241
AclWI GGATC 2 cut(s) 115, 186
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 1 cut(s) 342
AfiI CCNNNNNNNGG 1 cut(s) 104
AgsI TTSAA 1 cut(s) 337
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 2 cut(s) 235, 383
AluI AGCT 2 cut(s) 235, 383
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 2 cut(s) 115, 186
ApeKI GCWGC 1 cut(s) 35
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
AspLEI GCGC 2 cut(s) 269, 352
AspS9I GGNCC 2 cut(s) 172, 461
AsuHPI GGTGA 1 cut(s) 418
AvaII GGWCC 2 cut(s) 172, 461
BbsI GAAGAC 2 cut(s) 156, 314
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 176
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 1 cut(s) 368
BfaI CTAG 2 cut(s) 197, 236
BfuAI ACCTGC 1 cut(s) 343
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
Bme1390I CCNGG 1 cut(s) 176
Bme18I GGWCC 2 cut(s) 172, 461
BmgT120I GGNCC 2 cut(s) 172, 461
BmiI GGNNCC 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 2 cut(s) 20, 304
BpiI GAAGAC 2 cut(s) 156, 314
BpuEI CTTGAG 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 120, 185
BseBI CCWGG 1 cut(s) 176
BseGI GGATG 2 cut(s) 319, 485
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 2 cut(s) 120, 185
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 1 cut(s) 47
BsgI GTGCAG 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 104
BsmAI GTCTC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 216
Bso31I GGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 4 cut(s) 107, 144, 178, 408
BspACI CCGC 1 cut(s) 241
BspCNI CTCAG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 173
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 2 cut(s) 115, 186
BspTNI GGTCTC 1 cut(s) 368
BsrI ACTGG 2 cut(s) 120, 185
BssMI GATC 4 cut(s) 107, 144, 178, 408
BssNAI GTATAC 1 cut(s) 398
Bst1107I GTATAC 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 1 cut(s) 7
BstAPI GCANNNNNTGC 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 1 cut(s) 344
BstF5I GGATG 2 cut(s) 319, 485
BstHHI GCGC 2 cut(s) 269, 352
BstKTI GATC 4 cut(s) 110, 147, 181, 411
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 4 cut(s) 107, 144, 178, 408
BstMWI GCNNNNNNNGC 2 cut(s) 44, 356
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 47
BstV2I GAAGAC 2 cut(s) 156, 314
BstX2I RGATCY 1 cut(s) 107
BstYI RGATCY 1 cut(s) 107
BstZ17I GTATAC 1 cut(s) 398
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 2 cut(s) 319, 485
BtsIMutI CAGTG 1 cut(s) 12
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 1 cut(s) 361
CciI TCATGA 1 cut(s) 435
CfoI GCGC 2 cut(s) 269, 352
Cfr13I GGNCC 2 cut(s) 172, 461
Csp6I GTAC 1 cut(s) 341
CviAII CATG 3 cut(s) 154, 318, 436
CviJI RGCY 4 cut(s) 139, 235, 383, 482
CviKI_1 RGCY 4 cut(s) 139, 235, 383, 482
CviQI GTAC 1 cut(s) 341
DdeI CTNAG 1 cut(s) 344
DpnI GATC 4 cut(s) 109, 146, 180, 410
DpnII GATC 4 cut(s) 107, 144, 178, 408
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 2 cut(s) 172, 461
Eco57I CTGAAG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 174
EcoT22I ATGCAT 2 cut(s) 216, 319
FaeI CATG 3 cut(s) 157, 321, 439
FaiI YATR 8 cut(s) 93, 104, 155, 216, 218, 319, 398, 437
FatI CATG 3 cut(s) 153, 317, 435
FauNDI CATATG 1 cut(s) 216
FbaI TGATCA 1 cut(s) 408
FblI GTMKAC 1 cut(s) 397
Fnu4HI GCNGC 1 cut(s) 36
FokI GGATG 2 cut(s) 326, 472
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 2 cut(s) 197, 236
GlaI GCGC 2 cut(s) 268, 351
GluI GCNGC 1 cut(s) 36
HhaI GCGC 2 cut(s) 269, 352
Hin1II CATG 3 cut(s) 157, 321, 439
Hin6I GCGC 2 cut(s) 267, 350
HinP1I GCGC 2 cut(s) 267, 350
HinfI GANTC 1 cut(s) 442
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 2 cut(s) 209, 327
Hpy188III TCNNGA 3 cut(s) 125, 305, 436
Hpy8I GTNNAC 1 cut(s) 398
HpyAV CCTTC 3 cut(s) 14, 67, 397
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4V TGCA 4 cut(s) 214, 230, 317, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 356
HpyF3I CTNAG 1 cut(s) 344
Hsp92II CATG 3 cut(s) 157, 321, 439
HspAI GCGC 2 cut(s) 267, 350
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 4 cut(s) 107, 144, 178, 408
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 8 cut(s) 62, 110, 133, 154, 161, 188, 198, 338
Lsp1109I GCAGC 1 cut(s) 47
LweI GCATC 2 cut(s) 20, 304
MaeI CTAG 2 cut(s) 197, 236
MalI GATC 4 cut(s) 109, 146, 180, 410
MboI GATC 4 cut(s) 107, 144, 178, 408
MboII GAAGA 4 cut(s) 161, 295, 314, 451
MflI RGATCY 1 cut(s) 107
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 1 cut(s) 417
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 4 cut(s) 321, 363, 415, 472
Mph1103I ATGCAT 2 cut(s) 216, 319
MseI TTAA 2 cut(s) 134, 189
MspR9I CCNGG 1 cut(s) 176
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 44, 356
NdeI CATATG 1 cut(s) 216
NdeII GATC 4 cut(s) 107, 144, 178, 408
NlaIII CATG 3 cut(s) 157, 321, 439
NlaIV GGNNCC 1 cut(s) 173
NsiI ATGCAT 2 cut(s) 216, 319
NspI RCATGY 1 cut(s) 321
PagI TCATGA 1 cut(s) 435
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 174
PkrI GCNGC 1 cut(s) 37
PpuMI RGGWCCY 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 173
PspPI GGNCC 2 cut(s) 172, 461
PspPPI RGGWCCY 1 cut(s) 172
PsuI RGATCY 1 cut(s) 107
RsaI GTAC 1 cut(s) 342
RsaNI GTAC 1 cut(s) 341
SaqAI TTAA 2 cut(s) 134, 189
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 4 cut(s) 107, 144, 178, 408
Sau96I GGNCC 2 cut(s) 172, 461
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 1 cut(s) 251
SetI ASST 5 cut(s) 229, 237, 357, 385, 408
SfaNI GCATC 2 cut(s) 20, 304
SinI GGWCC 2 cut(s) 172, 461
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 1 cut(s) 417
SsiI CCGC 1 cut(s) 241
SspMI CTAG 2 cut(s) 197, 236
StyD4I CCNGG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 7
TaqI TCGA 2 cut(s) 147, 386
TasI AATT 1 cut(s) 417
TatI WGTACW 1 cut(s) 340
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 2 cut(s) 134, 189
Tru9I TTAA 2 cut(s) 134, 189
TscAI CASTG 1 cut(s) 12
TseI GCWGC 1 cut(s) 35
TspDTI ATGAA 3 cut(s) 452, 470, 474
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 2 cut(s) 172, 461
XceI RCATGY 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 397
XspI CTAG 2 cut(s) 197, 236
Zsp2I ATGCAT 2 cut(s) 216, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.