pycom10g17840

At4g26485-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000364
Physical Location & Seq
Forward (+)
21025172 .. 21027327
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 735 bp
ATGGAAGTGTTTGGCTTTGAGAAAAGGATTAAGAATTACAGCAGCTACCAGAAGATACTGTTAGTGGGAGAAGGAGACTTCTCTTTTGCCGTTTGCTTAGCCAGAGCTTTTGGCTTTGCTTTCAACATGGTTGCCACTTCTCTTGACTCCAGAGAGTCGTTAATGGTGAATTATTCAAAAGCTATGAGCAATGTGAAGGAATTGGAGTCCATGGGATGCATTGTATTGCATGAGGTGGATGTCCATAGCATGAGCAGGCACCCTTTCCTGATTAGTGTACGCTTCGATCGCATAATCTATAATTTTCCTCACGCCGGTTACTTGCATGGCTCTGAACGCAACAAGTTACAGATTTGGTTTCATCAGCATTTGGTCAGGGGATTCTTTGAGAATGCAAGGGAAATGCTGACCGGAGTAGGAGAAATTCATGTGACACACAAGACAACATTTCCTTTCAGTGAATGGAAAATAGTGGAGTTAGCTTATATGGCTGGATTATATTTGGTTCATGAAGAACAATTCTTGATATGGGATTATCCAGGTTATGAAAACAAGAGAGGAGCTGGGATGTGTGATCAAACTTTTCCTATTGGAAGGTCTAGCACCTTCAAATTTGCCAGAATGTTATTGTTGTACCGTTCTACCACTTCTGGTTTCCACCTTGGCGCTACTTCTTCGGGTCCAGGGCCTGGATGGGATACGTGTCCTCCCATGCAACTGCCATTTTACGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2431

Protein Analysis

245

Amino Acids

27.95

Weight (kDa)

7.18

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BMT5-like PF10354 20 - 186 3.7e-51 rRNA (uridine-N3-)-methyltransferase BTM5-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000364)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26485
fragaria_vesca FvH4_3g14860 FvH4_3g14860 FvH4_3g14861 FvH4_3g14862 FvH4_3g14862 FvH4_3g14880 FvH4_3g14880 FvH4_3g14890 FvH4_3g14891 FvH4_3g14892 FvH4_3g14901 FvH4_3g14902
malus_domestica MD05G1233500.v1.1 MD05G1233600.v1.1 MD10G1209900.v1.1 MD10G1210000.v1.1 MD10G1210100.v1.1 MD10G1210300.v1.1 MD10G1210400.v1.1
prunus_persica Prupe.4G132900_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133200_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133300_v2.0.a1 Prupe.4G133400_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133500_v2.0.a1 Prupe.4G133600_v2.0.a1 Prupe.4G133600_v2.0.a1
pyrus_communis pycom05g21110 pycom05g21120 pycom10g17840
rosa_chinensis RchiOBHm_Chr5g0024361 RchiOBHm_Chr5g0024391 RchiOBHm_Chr5g0024411 RchiOBHm_Chr5g0024431 RchiOBHm_Chr5g0024451 RchiOBHm_Chr5g0024461 RchiOBHm_Chr5g0024471 RchiOBHm_Chr5g0024481 RchiOBHm_Chr5g0024501 RchiOBHm_Chr5g0024511
rosa_laevigata RLG00000032799 RLG00000032800 RLG00000032801 RLG00000032802 RLG00000032803
rosa_multiflora Rmu_sc0000761.1_g000010 Rmu_sc0000761.1_g000014 Rmu_sc0000761.1_g000018 Rmu_sc0000761.1_g000025 Rmu_sc0005861.1_g000002 Rmu_sc0005861.1_g000004 Rmu_sc0005861.1_g000007 Rmu_sc0011809.1_g000001 Rmu_sc0011809.1_g000006
rosa_roxburghii Rroxscaffold_1G00054740 Rroxscaffold_1G00054750 Rroxscaffold_1G00054760 Rroxscaffold_1G00054770 Rroxscaffold_1G00054780 Rroxscaffold_1G00054790 Rroxscaffold_1G00054840 Rroxscaffold_1G00055860 Rroxscaffold_3G00237770 Rroxscaffold_6G00389330
rosa_rugosa Rorug05G0083300 Rorug05G0083400 Rorug05G0083400
rosa_samantha Rh5AG174100 Rh5AG174300 Rh5AG174600 Rh5AG174700 Rh5AG175000 Rh5AG175300 Rh5BG171000 Rh5BG171400 Rh5BG171500 Rh5BG171600 Rh5BG171700 Rh5BG172100 Rh5BG172300 Rh5BG172500 Rh5CG187400 Rh5CG187700 Rh5CG187900 Rh5CG188000 Rh5CG188200 Rh5CG188400 Rh5CG188500 Rh5CG188600 Rh5DG173200 Rh5DG173400 Rh5DG173500 Rh5DG173700 Rh5DG173900 Rh5DG174000 Rh5DG174100
rosa_wichuraiana Rw5G015700 Rw5G015730 Rw5G015740 Rw5G015770 Rw5G015790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 258
AccB7I CCANNNNNTGG 1 cut(s) 689
AcsI RAATTY 2 cut(s) 423, 611
AfaI GTAC 2 cut(s) 279, 635
AfiI CCNNNNNNNGG 2 cut(s) 314, 689
AflIII ACRYGT 1 cut(s) 701
AgsI TTSAA 3 cut(s) 124, 177, 610
AjnI CCWGG 3 cut(s) 538, 682, 688
AluBI AGCT 5 cut(s) 45, 107, 182, 482, 563
AluI AGCT 5 cut(s) 45, 107, 182, 482, 563
Alw26I GTCTC 1 cut(s) 69
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 1 cut(s) 686
ApeKI GCWGC 1 cut(s) 42
ApoI RAATTY 2 cut(s) 423, 611
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 2 cut(s) 680, 686
AsuHPI GGTGA 1 cut(s) 178
AvaII GGWCC 1 cut(s) 680
BanI GGYRCC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 54
BccI CCATC 1 cut(s) 687
BceAI ACGGC 1 cut(s) 74
BciT130I CCWGG 3 cut(s) 540, 684, 690
BciVI GTATCC 1 cut(s) 691
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 669
BfuI GTATCC 1 cut(s) 691
BisI GCNGC 1 cut(s) 43
BlpI GCTNAGC 1 cut(s) 97
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 3 cut(s) 540, 684, 690
Bme18I GGWCC 1 cut(s) 680
BmgT120I GGNCC 2 cut(s) 680, 686
BmiI GGNNCC 2 cut(s) 260, 681
BmrFI CCNGG 3 cut(s) 540, 684, 690
BmsI GCATC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 133
Bpu1102I GCTNAGC 1 cut(s) 97
BsaAI YACGTR 1 cut(s) 702
BsaJI CCNNGG 3 cut(s) 210, 661, 683
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 691, 721
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 689
Bse118I RCCGGY 1 cut(s) 314
Bse3DI GCAATG 1 cut(s) 196
BseBI CCWGG 3 cut(s) 540, 684, 690
BseDI CCNNGG 3 cut(s) 210, 661, 683
BseGI GGATG 4 cut(s) 221, 244, 573, 698
BseLI CCNNNNNNNGG 2 cut(s) 314, 689
BseMI GCAATG 1 cut(s) 196
BseRI GAGGAG 1 cut(s) 573
BseXI GCAGC 1 cut(s) 54
BseYI CCCAGC 1 cut(s) 563
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 1 cut(s) 688
BshNI GGYRCC 1 cut(s) 258
BsiEI CGRYCG 1 cut(s) 289
BsiSI CCGG 2 cut(s) 315, 411
BslI CCNNNNNNNGG 2 cut(s) 314, 689
BsmAI GTCTC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 1 cut(s) 688
Bsp143I GATC 2 cut(s) 286, 574
Bsp1720I GCTNAGC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 210
BspANI GGCC 1 cut(s) 688
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 260, 681
BspT107I GGYRCC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 210, 661, 683
BssMI GATC 2 cut(s) 286, 574
BssT1I CCWWGG 2 cut(s) 210, 661
Bst2UI CCWGG 3 cut(s) 540, 684, 690
Bst4CI ACNGT 2 cut(s) 60, 638
BstBAI YACGTR 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 1 cut(s) 97
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 4 cut(s) 221, 244, 573, 698
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 2 cut(s) 289, 577
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 2 cut(s) 286, 574
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 3 cut(s) 288, 336, 488
BstNI CCWGG 3 cut(s) 540, 684, 690
BstSCI CCNGG 3 cut(s) 538, 682, 688
BstV1I GCAGC 1 cut(s) 54
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 1 cut(s) 688
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 4 cut(s) 221, 244, 573, 698
BtsIMutI CAGTG 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 257
CaiI CAGNNNCTG 1 cut(s) 689
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 668
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 2 cut(s) 680, 686
Csp6I GTAC 2 cut(s) 278, 634
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviAII CATG 8 cut(s) 127, 211, 230, 250, 326, 428, 509, 712
CviQI GTAC 2 cut(s) 278, 634
DdeI CTNAG 1 cut(s) 97
DpnI GATC 2 cut(s) 288, 576
DpnII GATC 2 cut(s) 286, 574
Eco130I CCWWGG 2 cut(s) 210, 661
Eco47I GGWCC 1 cut(s) 680
EcoO109I RGGNCCY 1 cut(s) 686
EcoRII CCWGG 3 cut(s) 538, 682, 688
EcoT14I CCWWGG 2 cut(s) 210, 661
EcoT22I ATGCAT 1 cut(s) 221
ErhI CCWWGG 2 cut(s) 210, 661
FaeI CATG 8 cut(s) 130, 214, 233, 253, 329, 431, 512, 715
FatI CATG 8 cut(s) 126, 210, 229, 249, 325, 427, 508, 711
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 4 cut(s) 228, 251, 580, 705
Fsp4HI GCNGC 1 cut(s) 43
FspBI CTAG 1 cut(s) 600
GlaI GCGC 1 cut(s) 667
GluI GCNGC 1 cut(s) 43
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 1 cut(s) 688
HapII CCGG 2 cut(s) 315, 411
HhaI GCGC 1 cut(s) 668
Hin1II CATG 8 cut(s) 130, 214, 233, 253, 329, 431, 512, 715
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HinfI GANTC 4 cut(s) 146, 155, 206, 381
HpaII CCGG 2 cut(s) 315, 411
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 5 cut(s) 143, 150, 268, 509, 523
Hpy8I GTNNAC 1 cut(s) 278
HpyAV CCTTC 4 cut(s) 65, 190, 588, 616
HpyCH4III ACNGT 2 cut(s) 60, 638
HpyCH4IV ACGT 1 cut(s) 701
HpyCH4V TGCA 5 cut(s) 219, 229, 325, 395, 715
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 336, 488
HpyF3I CTNAG 1 cut(s) 97
HpySE526I ACGT 1 cut(s) 701
Hsp92II CATG 8 cut(s) 130, 214, 233, 253, 329, 431, 512, 715
HspAI GCGC 1 cut(s) 666
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 2 cut(s) 286, 574
LmnI GCTCC 1 cut(s) 560
Lsp1109I GCAGC 1 cut(s) 54
LweI GCATC 1 cut(s) 206
MaeI CTAG 1 cut(s) 600
MaeII ACGT 1 cut(s) 701
MaeIII GTNAC 3 cut(s) 317, 345, 430
MalI GATC 2 cut(s) 288, 576
MboI GATC 2 cut(s) 286, 574
MboII GAAGA 3 cut(s) 64, 524, 666
MluCI AATT 7 cut(s) 34, 169, 200, 301, 423, 518, 611
MlyI GAGTC 3 cut(s) 140, 164, 215
MnlI CCTC 4 cut(s) 226, 318, 551, 717
Mph1103I ATGCAT 1 cut(s) 221
MseI TTAA 2 cut(s) 30, 161
MspI CCGG 2 cut(s) 315, 411
MspR9I CCNGG 3 cut(s) 540, 684, 690
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 3 cut(s) 540, 684, 690
MwoI GCNNNNNNNGC 3 cut(s) 288, 336, 488
NcoI CCATGG 1 cut(s) 210
NdeII GATC 2 cut(s) 286, 574
NlaIII CATG 8 cut(s) 130, 214, 233, 253, 329, 431, 512, 715
NlaIV GGNNCC 2 cut(s) 260, 681
NmuCI GTSAC 1 cut(s) 430
NsiI ATGCAT 1 cut(s) 221
PagI TCATGA 1 cut(s) 508
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 381
PflMI CCANNNNNTGG 1 cut(s) 689
PkrI GCNGC 1 cut(s) 44
Ple19I CGATCG 1 cut(s) 289
PleI GAGTC 3 cut(s) 140, 163, 214
PpsI GAGTC 3 cut(s) 140, 163, 214
Ppu21I YACGTR 1 cut(s) 702
Psp6I CCWGG 3 cut(s) 538, 682, 688
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 3 cut(s) 538, 682, 688
PspN4I GGNNCC 2 cut(s) 260, 681
PspPI GGNCC 2 cut(s) 680, 686
PstNI CAGNNNCTG 1 cut(s) 689
PvuI CGATCG 1 cut(s) 289
RsaI GTAC 2 cut(s) 279, 635
RsaNI GTAC 2 cut(s) 278, 634
SaqAI TTAA 2 cut(s) 30, 161
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 2 cut(s) 286, 574
Sau96I GGNCC 2 cut(s) 680, 686
SchI GAGTC 3 cut(s) 140, 164, 215
ScrFI CCNGG 3 cut(s) 540, 684, 690
SfaNI GCATC 1 cut(s) 206
SinI GGWCC 1 cut(s) 680
Sse9I AATT 7 cut(s) 34, 169, 200, 301, 423, 518, 611
SspMI CTAG 1 cut(s) 600
StyD4I CCNGG 3 cut(s) 538, 682, 688
StyI CCWWGG 2 cut(s) 210, 661
TaaI ACNGT 2 cut(s) 60, 638
TaiI ACGT 1 cut(s) 704
TaqI TCGA 1 cut(s) 285
TasI AATT 7 cut(s) 34, 169, 200, 301, 423, 518, 611
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 2 cut(s) 30, 161
Tru9I TTAA 2 cut(s) 30, 161
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 5 cut(s) 350, 416, 497, 525, 561
TspRI CASTG 1 cut(s) 463
Van91I CCANNNNNTGG 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 2 cut(s) 423, 611
XcmI CCANNNNNNNNNTGG 1 cut(s) 690
XspI CTAG 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.